Effect of carbon and nitrogen sources on the production and carbohydrate composition of exopolysaccharide by submerged culture of pleurotus citrinopileatus

2020 ◽  
Vol 16 (2) ◽  
Author(s):  
C.-Y. Wu ◽  
Z.-C. Liang ◽  
C.-P. Lu ◽  
S.-H. Wu
Keyword(s):  
Submerged Culture ◽  
Nitrogen Sources ◽  
Carbohydrate Composition ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Pleurotus Citrinopileatus

The Screening of Optimal Ganoderma lucidum and the Effect of Medium Substrate on Bioactive Metabolite in Submerged Culture

2012 ◽  
Vol 554-556 ◽  
pp. 887-890
Author(s):  
Mei Lin Cui ◽  
Guo Qing He
Keyword(s):  
Yeast Extract ◽  
Ganoderma Lucidum ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
The Other ◽  
Carbon And Nitrogen Sources ◽  
Bioactive Metabolite ◽  
Carbon And Nitrogen ◽  
Other Hand

To determine an optimal G. lucidum strain, we made the production of mycelia biomass, EPS, IPS, IT, ET as indicators, among the five tested strains, the American G. lucidum was screened, and the production of mycelia biomass, EPS, IPS, IT, ET could reach 2.013g/100 ml, 11.2988 mg/100ml, 23.7800 mg/10ml, 45.5412 mg/100ml, 11.1417 mg/100ml, respectively. On the other hand, according to the screening of carbon and nitrogen sources as well as their concentration, the suitable carbon and nitrogen was 3% malt powder, 1.5% yeast extract. So we can use the fermentation culture for the following research, which was as follows：3% malt powder, 1.5%yeast extract, 0.3% KH2PO4, 0.15% MgSO4, 0.005% VB1.

scholarly journals Sıvı Kültürde Lepista Nuda Tarafından Miselyal Biyokütle ve Ekzopolisakkarit Üretimi İçin Karbon ve Azot Kaynaklarının Belirlenmesi

2018 ◽  
Vol 6 (5) ◽  
pp. 581
Author(s):  
Murat Özdal
Keyword(s):  
Biomass Production ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
Edible Mushroom ◽  
Mycelial Biomass ◽  
Carbon And Nitrogen Sources ◽  
Medium Carbon ◽  
Carbon And Nitrogen ◽  
Initial Medium ◽  
Optimized Conditions

In the present study, Lepista nuda, an edible mushroom, was used for the production of exopolysaccharide in the submerged culture. The effects of initial medium carbon and nitrogen sources on exopolysaccharide and mycelial biomass production by L. nuda IT strain were investigated. The highest exopolysaccharide production was obtained when culture parameters were used as initial medium 50 g/L glucose and 5 g/L yeast extract. As a result, 2.12 g/L exopolysaccharide and 12.3 g/L biomass production in optimized conditions at flask scales were achieved. This is the first study showing the production of exopolysaccharide in liquid culture of Lepista nuda.

Mycelial differentiation and spore formation by Streptomyces brasiliensis in submerged culture

2001 ◽  
Vol 47 (11) ◽  
pp. 1042-1047 ◽  
Author(s):  
Begoña Rueda ◽  
Elisa M Miguélez ◽  
Carlos Hardisson ◽  
Manuel B Manzanal
Keyword(s):  
Electron Microscopy ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Ultrastructural Changes ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Solid Media ◽  
Aerial Hyphae

Streptomyces brasiliensis ATCC 23727 showed extensive sporulation when cultured in a liquid medium containing galactose and glutamic acid as carbon and nitrogen sources. Sporogenic hyphae formed under these conditions were morphologically similar and developmentally equivalent to aerial hyphae and metamorphosed into chains of spores by following a sequence of ultrastructural changes similar to that observed during growth on solid media. In addition, our electron microscopy study revealed two previously unrecognized aspects of hyphal development in streptomycetes: the formation of sporogenic hyphae was always preceded by changes in the structure of the nucleoid, and the sheath that characteristically covered these hyphae was not deposited coincidently with wall formation in the apical growing portion of the hypha.Key words: Streptomyces brasiliensis, mycelial differentiation, sporulation.

The production of constitutive invertase and inulinase by the mushroom Panaeolus papillonaceus in submerged culture

1985 ◽  
Vol 31 (9) ◽  
pp. 773-777 ◽  
Author(s):  
Khana Mukherjee (Chakraborty) ◽  
S. Sengupta
Keyword(s):  
Submerged Culture ◽  
Agaricus Bisporus ◽  
Nitrogen Sources ◽  
Invertase Activity ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Inulinase Activity ◽  
Temperature Optima ◽  
Optimum Production ◽  
Starch Medium

Five different mushrooms were screened for extracellular inulinase and invertase activities. The enzymes were found to be produced constitutively by the mushrooms, but the carbon source, glucose, sucrose, and starch, in the medium influenced the production of individual enzymes. The highest producers of inulinase and invertase were Panaeolus papillonaceus growing in starch medium and Agaricus bisporus growing in glucose medium, respectively. A medium for the optimum production of invertase and inulinase by P. papillonaceus was developed by determining the optimum concentrations of suitable carbon and nitrogen sources. Some of the physicochemical properties of the enzymes present in the culture filtrate were studied. Both of the activities have pH optima at 6.5 and temperature optima at 60 °C. Km values for both enzyme activities were found to be identical at 2.5 mg of substrate/mL, whereas the νm for the inulinase activity was more than two times greater than that for invertase activity.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

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