scholarly journals The effect of commercial herbal toothpastes on dental wear: a comparative evaluation by Optical coherence tomography

2021 ◽  
Vol 10 (11) ◽  
pp. e161101119583
Author(s):  
Pedro Romeu de Mendonça Vila Nova ◽  
Paulo Cardoso Lins Filho ◽  
Marlon Ferreira Dias ◽  
Hilcia Mezzalira Teixeira ◽  
Silvana Orestes Cardoso ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Tooth Wear ◽  
Positive Control ◽  
Negative Control ◽  
Optical Coherence ◽  
Surface Wear ◽  
Dental Wear ◽  
Dentin Surface ◽  
Nail Polish ◽  
Enamel Wear

The purpose of this study was to compare the tooth wear in function of the use of different commercial herbal toothpastes through the analysis of Optical Coherence Tomography (OCT). Twenty bovine teeth were obtained and distributed in 4 groups (n = 5) according to the dentifrice used: G1: Captive Nature (Chamomile, Xylitol, Juá and Salvia); G2: Suavetex Content (Turmeric); G3: Colgate Triple Action (positive control); G4: distilled water (negative control). The samples were painted in the half of the fragment with nail polish so that only half of the fragment was brushed. The simulated brushing (20,000 cycles) was performed with linear movements, under static axial load of 200g and speed of 4.5 cycles per second. After this step, an analysis was performed through OCT and and the images obtained were evaluated to identify possible changes in the specimen surface. According to the qualitative analysis of the OCT images, enamel wear was not observed, since all measurements were null. Regarding the evaluation of dentin, surface wear was observed in all groups except G4, but G3 had the highest number of samples with surface wear around 21.32%. All dentifrices had abrasive wear on the dentin surface to a greater or lesser extent, but there was no wear on the enamel surface.

scholarly journals Diagnosis of Occlusal Tooth Wear Using 3D Imaging of Optical Coherence Tomography Ex Vivo

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 6016
Author(s):  
Misa Kashiwa ◽  
Yasushi Shimada ◽  
Alireza Sadr ◽  
Masahiro Yoshiyama ◽  
Yasunori Sumi ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Digital Radiography ◽  
3D Imaging ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Tooth Wear ◽  
Laser Scanning Microscopy ◽  
Optical Coherence ◽  
Significant Difference ◽  
Enamel Wear

The aim of this study was to assess the utility of 3D imaging of optical coherence tomography (OCT) for the diagnosis of occlusal tooth wear ex vivo. Sixty-three extracted human molars with or without visible tooth wear were collected to take digital intraoral radiography and 3D OCT images. The degree of tooth wear was evaluated by 12 examiners and scored using 4-rank scale: 1—slight enamel wear; 2—distinct enamel wear; 3—tooth wear with slight dentin exposure; 4—tooth wear with distinct involvement of dentin. The degree of tooth wear was validated by the histological view of confocal laser scanning microscopy (CLSM). The sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic analysis were calculated. Diagnostic accuracy was compared with the agreement with CLSM observation using weighted kappa. The results were statistically analyzed at a significance level of α = 0.05. Three-dimensional OCT showed significantly higher sensitivity (p < 0.05) for all the diagnostic thresholds of enamel wear and dentin exposure than digital radiography (0.82, 0.85, and 0.79 vs. 0.56, 0.52, and 0.57, respectively). Three-dimensional OCT showed higher AUC and kappa coefficients than digital radiography (p < 0.05), where mean AUC and Kappa values were 0.95 and 0.76 for OCT and 0.92 and 0.47 for radiography, respectively. No significant difference of specificity was observed (p > 0.05). Three-dimensional OCT could visualize and estimate the degree of tooth wear and detect the dentin exposure at the tooth wear surface accurately and reproducibly. Consequently, a new guideline for tooth wear assessment can be proposed using OCT.

Evaluating Dentin Surface Treatments for Resin-Modified Glass Ionomer Restorative Materials

2013 ◽  
Vol 38 (4) ◽  
pp. 429-438 ◽  
Author(s):  
TA Imbery ◽  
A Namboodiri ◽  
A Duncan ◽  
R Amos ◽  
AM Best ◽  
...  
Keyword(s):  
Testing Machine ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Surface Treatments ◽  
Band Matrices ◽  
Dentin Surface ◽  
Occlusal Surfaces ◽  
Significant Difference

SUMMARY This in vitro study evaluated the effect of six surface treatments on the shear bond strength of three resin-modified glass ionomers (RMGIs) to dentin. Occlusal surfaces of caries-free third molars were reduced to expose only dentin. Surface treatments were smear layer intact (negative control), Cavity Conditioner, EDTA, Ketac Primer, Self Conditioner, and etching with 35% phosphoric acid followed by the application of Optibond Solo Plus. Filtek Z250 composite resin bonded with Optibond Solo Plus served as a positive control. Conditioning agents were used according to the manufacturers' instructions. After surface treatments, Fuji II LC, Riva LC, Ketac Nano, and Filtek Z250 were placed in copper-band matrices 5 mm in diameter and 2 mm in height and were light-cured for 20 seconds. Specimens were stored in 100% humidity for 24 hours, after which they were placed in deionized water for 24 hours at 37°C. They were then tested under shear forces in an Instron Universal Testing Machine at a crosshead speed of 0.5 mm/min. A two-way analysis of variance and Tukey honestly significant difference statistical analyses (p&lt;0.05) indicated significant interaction between RMGIs and conditioning agents. Acid etching followed by Optibond Solo Plus provided highest bond strengths for all three RMGIs, which were not statistically different from the positive control.

Enamel wear evolution: Evaluation using statistical mixed models for 2D profilometry data

2018 ◽  
Vol 233 (8) ◽  
pp. 1500-1509
Author(s):  
Agnes Batista Meireles ◽  
Janaína Luciana Alvernaz Marques Ferreira ◽  
Flávia de Souza Bastos ◽  
Letícia Bonato ◽  
Estevam Barbosa de Las Casas
Keyword(s):  
Mixed Models ◽  
Tooth Wear ◽  
Mixed Effects ◽  
Time Dependent ◽  
Two Dimensional ◽  
Data Correlation ◽  
Dental Wear ◽  
Constant Rate ◽  
Enamel Wear ◽  
Established Technique

Two-dimensional profilometry is an inexpensive well-established technique to identify indicators of tooth wear. Four surface parameters ( Ra, Rq, Rsk, and Rku) were selected in this work to evaluate worn dental surfaces using two-dimensional profilometry. Eight subjects with dental wear were molded four times with a 15 days interval. To verify data correlation, a mixed-effects model was adjusted. The results indicated that Ra, Rq, Rsk are time dependent, while Rku remained constant. Rsk and Rku values were useful to identify surfaces. Skewness had oscillatory variations and Rku was tending toward a constant rate for a longer period. Two regimens were suggested: (1) transient and (2) self-limiting. Final variation rates indicated that, although enamel wear transiently modifies the surface, it maintains its isotropic characteristic: symmetrical and approximately normal asperities distribution.

Effect of Phosphorylated Chitosan on Dentin Erosion: An in vitro Study

2018 ◽  
Vol 52 (5) ◽  
pp. 378-386 ◽  
Author(s):  
Ana Paula C.A. Beltrame ◽  
Dakota Suchyta ◽  
Islam Abd Alraheam ◽  
Anas Mohammed ◽  
Mark Schoenfisch ◽  
...  
Keyword(s):  
Modulus Of Elasticity ◽  
In Vitro Study ◽  
Chitosan Solution ◽  
Dental Erosion ◽  
Positive Control ◽  
Negative Control ◽  
Favorable Effect ◽  
Dentin Surface ◽  
Phosphate Buffered Saline

The aim of this study was to evaluate the antierosive effect of phosphorylated chitosan in dentin. Bovine dentin specimens were randomly distributed into the following groups: (1) no treatment (NoTx/negative control), (2) phosphate-buffered saline solution (PBS), (3) AmF/NaF/SnCl2 (positive control), (4) 0.5% chitosan solution (Chi), (5) 0.5% neutral phosphorylated (NP)-Chi, and (6) 0.5% alkaline phosphorylated (AP)-Chi. The specimens were submitted to de-remineralization treatment cycles for 5 days: 0.5% citric acid (2 min), remineralizing solution (30 min), and surface treatment according to assigned groups (2 min, 6×/day). The loss of dentin surface was measured by profilometry. Hardness and modulus of elasticity were measured using a nanoindenter equipped with a Berkovich diamond tip. The dentin surface was analyzed by scanning electron microscopy (SEM). The largest loss of dentin was observed in the No Tx and PBS groups (approx. 25 µm). The group treated with AmF/NaF/SnCl2 showed less loss of dentin (67% reduction vs. NoTx and PBS), followed by the groups treated with NP-Chi and AP-Chi (33% reduction), and Chi (18% reduction). Nanohardness and modulus of elasticity were similar in the NoTx and PBS groups, with a small increase in stiffness in all other groups. SEM revealed that the experimental solution of AP-Chi had a favorable effect on maintaining the integrity of collagen fibrils. AmF/NaF/SnCl2 showed a preserved mineralized collagen surface. Further studies are warranted to explore this nontoxic phosphorylated chitosan polymer as an effective agent in the prevention and treatment of dental erosion.

In vitro longitudinal evaluation of enamel wear by cross-polarization optical coherence tomography

2019 ◽  
Vol 35 (10) ◽  
pp. 1464-1470 ◽  
Author(s):  
Maryam A. Alghilan ◽  
Frank Lippert ◽  
Jeffrey A. Platt ◽  
George J. Eckert ◽  
Carlos González-Cabezas ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Cross Polarization ◽  
Longitudinal Evaluation ◽  
Enamel Wear

Early detection of tooth wear by en-face optical coherence tomography

2009 ◽  
Author(s):  
Corina Mărcăuteanu ◽  
Meda Negrutiu ◽  
Cosmin Sinescu ◽  
Eniko Demjan ◽  
Mike Hughes ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Early Detection ◽  
Tooth Wear ◽  
Optical Coherence ◽  
En Face

Towards using spectral domain optical coherence tomography for dental wear monitoring

2014 ◽  
Author(s):  
Corina Mărcăuţeanu ◽  
Adrian Bradu ◽  
Cosmin Sinescu ◽  
Florin I. Topală ◽  
Meda Lavinia Negruţiu ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Dental Wear ◽  
Wear Monitoring

Safety and In Situ Antierosive Effect of CaneCPI-5 on Dental Enamel

2021 ◽  
pp. 002203452110115
Author(s):  
V.T. Pelá ◽  
J.G.Q. Lunardelli ◽  
C.K. Tokuhara ◽  
C.C. Gironda ◽  
N.D.G. Silva ◽  
...  
Keyword(s):  
Dental Enamel ◽  
Erosive Wear ◽  
Tooth Wear ◽  
Positive Control ◽  
Negative Control ◽  
Mitochondrial Activity ◽  
Double Blind ◽  
Enamel Erosion ◽  
Bovine Enamel

The sugarcane cystatin (CaneCPI-5) was recently cloned and showed strong binding force to dental enamel and protection against initial erosion. However, evaluations on its safety and efficacy in a situation closer to the clinical condition are necessary. In the present study we analyzed 1) the cytotoxicity of CaneCPI-5 on human gingival fibroblasts (HGFs); 2) the ability of CaneCPI-5 to reduce enamel erosion and erosion+abrasion in situ. In part 1, HGFs were treated with CaneCPI-5 (0.025, 0.05, 0.1, 0.5 or 1.0 mg/mL) or no treatment (control). The cytotoxicity was assessed after 60 s and 24 h by mitochondrial activity (MTT), confocal microscopy, and hematoxylin/eosin staining. In part 2, 15 volunteers participated in a double-blind crossover protocol consisting of 3 phases, according to the following treatments: 1) 0.1 mg/mL CaneCPI-5; 2) SnCl2/NaF/AmF (Elmex; positive control); 3) water (negative control). The volunteers wore an appliance containing 4 bovine enamel specimens for 5 d. Each day, the specimens were individually treated with 50 µL of the tested solutions per 60 s and then subjected to erosive challenges (0.1% citric acid, pH 2.5, for 90 s, 4 times per day). After the first and last erosive challenge each day, 2 samples were abraded (toothbrushing, 15 s). Enamel wear was measured by contact profilometry. One or two-way analysis of variance (ANOVA)/Tukey’s or Sidak’s tests ( P < 0.05) were applied. Regardless of the concentration and the experimental time, CaneCPI-5 did not decrease the cell viability compared to the negative control ( P < 0.05). Erosion+abrasion led to significantly greater wear compared to erosion only. For both conditions, the lowest wear was found for SnCl2 and CaneCPI-5, which did not differ significantly from each other, but showed significant protection when compared to the negative control. In conclusion, CaneCPI-5 is safe on HGFs and reduces enamel erosive wear to the same extent as a commercial solution used to control erosive tooth wear (ETW).

scholarly journals Erosive tooth wear inhibition by hybrid coatings with encapsulated fluoride and stannous ions

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Sávio José Cardoso Bezerra ◽  
Ítallo Emídio Lira Viana ◽  
Idalina Vieira Aoki ◽  
Maria Angela Pita Sobral ◽  
Alessandra Buhler Borges ◽  
...  
Keyword(s):  
Tooth Wear ◽  
Dental Erosion ◽  
Positive Control ◽  
Negative Control ◽  
Hybrid Coating ◽  
Deionized Water ◽  
Hybrid Coatings ◽  
Control Surface ◽  
Upward Trend ◽  
Optical Profilometer

AbstractThis study aimed to formulate a hybrid coating material (HC) and to modify this HC with fluoride (NaF) and stannous (SnCl2) ions, directly or encapsulated in nano containers, testing the effects of these materials against dental erosion and erosion–abrasion. Enamel and dentin specimens were treated with the HCs, and then tested in erosion or erosion–abrasion cycling models of 5 days (n = 10 for each substrate, for each model). Deionized water was the negative control, and a fluoride varnish, the positive control. Surface loss (SL, in µm) was evaluated with an optical profilometer, and data were statistically analyzed (α = 0.05). For enamel, in erosion, the positive control and HC without additives showed significantly lower SL than the negative control (p = 0.003 and p = 0.001). In erosion–abrasion, none of the groups differed from the negative control (p > 0.05). For dentin, in erosion, the positive control, HC without additives, HC with non-encapsulated F, and HC with encapsulated F + Sn showed lower SL than the negative control (p < 0.05). In erosion–abrasion, none of the groups differed significantly from the negative control (p < 0.05). HC without additives showed a promising potential for protecting the teeth against dental erosion (with upward trend for improved protection on dentin), but not against erosion–abrasion. The presence of additives did not improve the protective effect of the HC, on both substrates.

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