Clinical variations observed among the main hemoparasitosis caused by Rhipicephalus sanguineus in dogs

Hemoparasitosis are diseases of great importance in the practice of a small animal’s veterinarian, due to their high number of cases in the clinical routine of these species, and the wide occurrence of their vectors. Related to hemoparasitosis in dogs, the tick Rhipicephalus sanguineus has the greatest importance due to parasitizing dogs, perpetuating hemoparasitosis in this host group. The present work describes a literature review about the three main hemoparasitosis in the routine of a veterinarian in Brazil, ehrlichiosis, caused by Ehrlichia canis, babesiosis caused by the protozoa Babesia canis and Babesia gibsoni, and anaplasmosis caused by Anaplasma platys, comparing them regarding the differences between clinical signs and laboratory alterations, such as the different types of anemia caused by these conditions that culminate in clinical signs related to them. It also aims to describe the similarity in the diagnostic techniques used and infection treatments, which in all cases is used tetracycline, the most commonly used is doxycycline, and highlight the need for further studies about this topic.

Download Full-text

HEMOPARASITOSE EM UMA CANINA- RELATO DE CASO

10.51161/rems/1888 ◽

2021 ◽

Author(s):

Bruna Degregori ◽

Geane Consorte ◽

Fernando Zorzi

Keyword(s):

Anaplasma Phagocytophilum ◽

Rhipicephalus Sanguineus ◽

Babesia Canis ◽

Anaplasma Platys ◽

Babesia Gibsoni

Introdução: A hemoparasitose é uma patologia de alta casuística na rotina clínica veterinária, desencadeada por bactérias, protozoários, helmintos, possuindo como principal transmissor o carrapato em períodos do ano de maior incidência de calor e umidade, tendo em vista o potencial zoonótico. A babesiose é causada pelos protozoários Babesia Canis e Babesia gibsoni transmitida pelo carrapato Rhipicephalus sanguineus, esses parasitas causam a hemólise das hemácias. Já a anaplasmose é oriunda de bactérias intracelulares Anaplasma platys ou Anaplasma phagocytophilum transmitidas pelo carrapato Rhipicephalus sanguineus, causando trombocitopenia. A rangeliose é ocasionada por um piroplasma Rangelia vitalli, transmitida por carrapatos Rhipicephalus sanguineus e Amblyomma aerolatum, se replicando nos eritrócitos. Objetivo: Relatar um caso clínico de uma canina. Relato de caso: canina castrada, sem raça definida, com 1 ano e dois meses, pesando 11,4 Kg, sendo atendida em uma clínica veterinária particular em Caxias do Sul/RS, apresentando apatia, hematoquezia, anorexia, prostração, epistaxe, sangramento em pontas de orelhas, mucosas hipocoradas com petéquias e febre. Durante o atendimento foi realizada coleta sanguínea para avaliação hematológica, esfregaço sanguíneo periférico da orelha, bioquímica sérica (creatinina, fosfatase alcalina, ureia, transaminase pirúvica) e urinálise, apresentando alterações, tais como anemia regenerativa, leucocitose por neutrofilia com desvio a esquerda, linfocitose, trombocitopenia, na urinálise houve presença de hemoglobina, leucócitos, proteinúria. No exame de esfregaço de sangue periférico apresentou-se negativo para hemoparasitas. O diagnóstico definitivo foi através da Reação em Cadeia da Polimerase (PCR), na qual evidenciou os agentes patogênicos de babesiose, rangeliose e anaplasmose. O tratamento instituído foi doxiciclina (5 mg/kg/q.12h durante 28 dias), azitromicina (10 mg/Kg/q.24h durante 10 dias), diaceturato de diminazeno (0,5ml/2Kg) repetindo em 14 dias a dose com prévia aplicação de atropina (0,044 mg/Kg), suplemento vitamínico mineral aminoácido (1 ml/q.24h). O animal se manteve bem clinicamente durante o tratamento, obtendo cura da enfermidade. Conclusão: Mesmo com o diagnóstico negativo de esfregaço sanguíneo periférico, com a suspeita clínica do animal foi solicitado PCR para confirmar a patologia, assim foi de suma importância a pesquisa de hemoparasitas pelos sinais clínicos do canino, juntamente do tratamento precoce e assertivo.

Download Full-text

Report of candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis canine natural infections in Massambaba restinga, Brazil

10.7287/peerj.preprints.27805v1 ◽

2019 ◽

Author(s):

Marcia Gonçalves Miranda ◽

Bruno Alberigi ◽

Flavya Mendes-de-Almeida ◽

Alexandre José Rodrigues Bendas ◽

Nadia Almosny ◽

...

Keyword(s):

Climate Changes ◽

Global Climate ◽

The Other ◽

Southeastern Brazil ◽

Babesia Canis ◽

Anaplasma Platys ◽

Babesia Gibsoni ◽

Global Climate Changes ◽

History Of ◽

Healthy Dogs

Background: Tick-borne diseases are frequent in the Southeastern section of Brazil. The most prevalent canine parasites diagnosed are Ehrlichia canis, Babesia gibsoni, Babesia canis and Anaplasma platys, although Mycoplasma haemocanis and candidatus Mycoplasma haematoparvum have been registered in the country. Methods: When two clinically healthy dogs from a suburban area of the state of Rio de Janeiro, Brazil with history of being heavily infested with ticks were examined at Idexx Reference Laboratories, California for tick panel check. Results: One dog harbored DNA of candidatus Mycoplasma haematoparvum and the other DNA of Mycoplasma haemocanis. Conclusions: These results suggest that monitoring for tick infestations and tick-borne parasites must be permanent in southeastern Brazil, especially due to global climate changes which may contribute to spread and increase the number of infections in the Country.

Download Full-text

Report of candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis canine natural infections in Massambaba restinga, Brazil

10.7287/peerj.preprints.27805 ◽

2019 ◽

Author(s):

Marcia Gonçalves Miranda ◽

Bruno Alberigi ◽

Flavya Mendes-de-Almeida ◽

Alexandre José Rodrigues Bendas ◽

Nadia Almosny ◽

...

Keyword(s):

Climate Changes ◽

Global Climate ◽

The Other ◽

Southeastern Brazil ◽

Babesia Canis ◽

Anaplasma Platys ◽

Babesia Gibsoni ◽

Global Climate Changes ◽

History Of ◽

Healthy Dogs

Download Full-text

Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612011000400004 ◽

2011 ◽

Vol 20 (4) ◽

pp. 274-280 ◽

Cited By ~ 8

Author(s):

Sabrina Castilho Duarte ◽

Juliana Alves Parente ◽

Maristela Pereira ◽

Célia Maria de Almeida Soares ◽

Guido Fontgalland Coelho Linhares

Keyword(s):

Molecular Similarity ◽

Clinical Signs ◽

Babesia Canis ◽

Babesia Gibsoni ◽

Phylogenetic Characterization ◽

Pcr Products ◽

High Degree ◽

Veterinary Hospital ◽

Babesia Canis Canis

The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of Goiás. Only samples presenting typical intraerythrocytic parasites were used in the study. These were subjected to DNA extraction and amplification of a fragment of the 18S rRNA, by means of PCR. The PCR products were purified and sequenced. Sequences were obtained from 35 samples but only 17 of these were kept after quality assessment. Similarity analysis using BLASTn demonstrated that all 17 sequences corresponded to B. canis vogeli. Analysis using the Mega4 software showed that the isolates of B. canis vogeli from dogs in Goiânia present a high degree of molecular similarity (99.2 to 100%) in comparison with other reference isolates from other regions of Brazil and worldwide, deposited in GenBank.

Download Full-text

Investigation of Tickborne Pathogens within Naturally Infected Brown Dog Tick (Ixodidae: Rhipicephalus Sanguineus) in Egypt by Light and Electron Microscopy

International Journal of Veterinary Science ◽

10.37422/ijvs/20.064 ◽

2020 ◽

Keyword(s):

Electron Microscopy ◽

Salivary Gland ◽

Light And Electron Microscopy ◽

Rhipicephalus Sanguineus ◽

Mammalian Host ◽

Babesia Canis ◽

Brown Dog Tick ◽

Transmission Electron ◽

Dog Tick ◽

Theileria Spp

Tick borne pathogens present a significant health challenge to animals and human because a single tick may transmit multiple pathogens to a mammalian host during feeding. The present study detected tick-borne pathogens from pet dogs. A total of 666 ticks were collected from 144 pet and sheltered dogs in Egypt from April to September 2018. For hemolymph, midgut and salivary gland smears 546 ticks were used as well as 360 egg smears from 120 female tick were examined by light microscope. The infected ticks were prepared for transmission electron microscopy (TEM). Ticks were identified; Rhipicephalus sanguineus. Light microscopy showed infection rates of 44.69%, 68.50% & 15.75%, in hemolymph, midgut and salivary gland, respectively. H. canis recorded the highest rates in hemolymph and midgut (35.89% & 49.82%, respectively), but Theileria spp. was the lowest (0.73% & 2.93%, respectively). In salivary gland smears, Babesia canis. was detected in 13.55% and Theileria spp. in 1.83%. Mixed infection in same tick was recorded in 4.76% &0.37% in midgut and salivary gland smears, respectively. Babesia canis stages were recovered from 15% of egg smears. R. sanguineus was natural infected by Babesia, Theileria, Hepatozoon and Anaplasma phagocytophilum as well as mixed infections of protozoa accompanied by a complicated sign of diseases and failure in accurate diagnosis.

Download Full-text

Double, triple or quadruple hits? Exploring the impact of cybercrime on victims in the Netherlands

International Review of Victimology ◽

10.1177/02697580211010692 ◽

2021 ◽

pp. 026975802110106

Author(s):

Raoul Notté ◽

E.R. Leukfeldt ◽

Marijke Malsch

Keyword(s):

Public Opinion ◽

Literature Review ◽

The Netherlands ◽

Public Discourse ◽

Moral Judgments ◽

Victim Blaming ◽

High Prevalence ◽

Different Types ◽

The Impact ◽

Insight Into

This article explores the impact of online crime victimisation. A literature review and 41 interviews – 19 with victims and 22 with experts – were carried out to gain insight into this. The interviews show that most impacts of online offences correspond to the impacts of traditional offline offences. There are also differences with offline crime victimisation. Several forms of impact seem to be specific to victims of online crime: the substantial scale and visibility of victimhood, victimisation that does not stop in time, the interwovenness of online and offline, and victim blaming. Victims suffer from double, triple or even quadruple hits; it is the accumulation of different types of impact, enforced by the limitlessness in time and space, which makes online crime victimisation so extremely invasive. Furthermore, the characteristics of online crime victimisation greatly complicate the fight against and prevention of online crime. Finally, the high prevalence of cybercrime victimisation combined with the severe impact of these crimes seems contradictory with public opinion – and associated moral judgments – on victims. Further research into the dominant public discourse on victimisation and how this affects the functioning of the police and victim support would be valuable.

Download Full-text

Traumatic Dissection of Arterial Cervical Vessels: Report of Two Cases and Literature Review

Arquivos Brasileiros de Neurocirurgia Brazilian Neurosurgery ◽

10.1055/s-0040-1718422 ◽

2020 ◽

Author(s):

Guilherme Finger ◽

Tobias Ludwig do Nascimento ◽

Julia Carolina Lusa Tessaro ◽

Eduarda Tanus Stefani ◽

Maria Eduarda Conte Gripa ◽

...

Keyword(s):

Literature Review ◽

Acute Phase ◽

Clinical Signs ◽

Artery Dissection ◽

Cervical Artery Dissection ◽

Cervical Artery

AbstractEven though traumatic dissection of cervical arterial vessels is the major cause of stroke among adults, it is still an underdiagnosed disease in neurosurgical emergencies, since most patients do not have or present subtle clinical signs in the acute phase. The authors report two interesting cases of cervical artery dissection with different traumatic mechanisms and present a broad literature review about this subject.

Download Full-text

Ontology Engineering for Gastric Dystemperament in Persian Medicine

Methods of Information in Medicine ◽

10.1055/s-0041-1735168 ◽

2021 ◽

Author(s):

Hassan Shojaee-Mend ◽

Haleh Ayatollahi ◽

Azam Abdolahadi

Keyword(s):

Literature Review ◽

Delphi Study ◽

Evaluation Study ◽

Mixed Methods Study ◽

Case Based Reasoning ◽

Second Stage ◽

New Concepts ◽

Persian Medicine ◽

Different Types ◽

Specification Document

Abstract Objective Developing an ontology can help collecting and sharing information in traditional medicine including Persian medicine in a well-defined format. The present study aimed to develop an ontology for gastric dystemperament in the Persian medicine. Methods This was a mixed-methods study conducted in 2019. The first stage was related to providing an ontology requirements specification document. In the second stage, important terms, concepts, and their relationships were identified via literature review and expert panels. Then, the results derived from the second stage were refined and validated using the Delphi method in three rounds. Finally, in the fourth stage, the ontology was evaluated in terms of consistency and coherence. Results In this study, 241 concepts related to different types of gastric dystemperament, diagnostic criteria, and treatments in the Persian medicine were identified through literature review and expert panels, and 12 new concepts were suggested during the Delphi study. In total, after performing three rounds of the Delphi study, 233 concepts were identified. Finally, an ontology was developed with 71 classes, and the results of the evaluation study revealed that the ontology was consistent and coherent. Conclusion In this study, an ontology was created for gastric dystemperament in the Persian medicine. This ontology can be used for designing future systems, such as case-based reasoning and expert systems. Moreover, the use of other evaluation methods is suggested to construct a more complete and precise ontology.

Download Full-text

Efeitos de diferentes doses de hiclato de doxiciclina nos parâmetros hematológicos de cães com erliquiose

Semina Ciências Biológicas e da Saúde ◽

10.5433/1679-0367.2016v37n1p53 ◽

2016 ◽

Vol 37 (1) ◽

pp. 53

Author(s):

Mariana Cristina Hoeppner Rondelli ◽

Reinaldo Juan Garrido Palacios Junior ◽

Igor Luiz Salardani Senhorello ◽

Áureo Evangelista Santana ◽

Mirela Tinucci-Costa

Keyword(s):

Nested Pcr ◽

Ehrlichia Canis ◽

Babesia Canis ◽

Anaplasma Platys ◽

Leptospira Spp

Resumo: Este estudo objetivou comparar os efeitos de duas doses de hiclato de doxiciclina sobre a contagem de hemácias, concentração de hemoglobina, hematócrito, contagem de leucócitos e plaquetas de cães com erliquiose. Foram estudados os grupos I, composto por cães saudáveis (n=6), negativos na sorologia para Ehrlichia canis e Leptospira spp., na PCR em tempo real para E. canis e Anaplasma platys e na semi–nested PCR para Babesia canis; grupos II (n=6) e III (n=6), compostos por cães com história clínica sugestiva, sorologia e/ou PCR em tempo real positivas para E. canis, e negativos na pesquisa de anticorpos anti-Leptospira spp., na PCR em tempo real para A. platys e na semi–nested PCR para B. canis. Os cães doentes foram tratados com hiclato de doxiciclina via oral a cada 12 horas por 30 dias (5 mg/kg, grupo II; 10 mg/kg, grupo III). Hemogramas foram feitos antes, decorridos 15 dias e aos 10 dias após o término dos tratamentos. Não houve diferenças entre os grupos nos tempos avaliados quanto às variáveis hemácias, hemoglobina, hematócrito e leucócitos. Observou-se diferença quanto aos trombócitos entre o grupo I e os grupos II e III (p<0,0001) no início do estudo. Aos 15 dias de tratamento, a média do grupo III foi menor que dos grupos I (p=0,008) e II (p=0,0007), indicando persistência da trombocitopenia, ausente no grupo II. Não houve diferença entre os grupos no tempo final, sugerindo que ambos os tratamentos promoveram aumento da contagem de plaquetas em cães naturalmente infectados por E. canis.

Download Full-text