scholarly journals HEMOPARASITOSE EM UMA CANINA- RELATO DE CASO

2021 ◽  
Author(s):  
Bruna Degregori ◽  
Geane Consorte ◽  
Fernando Zorzi
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Rhipicephalus Sanguineus ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Babesia Gibsoni

Introdução: A hemoparasitose é uma patologia de alta casuística na rotina clínica veterinária, desencadeada por bactérias, protozoários, helmintos, possuindo como principal transmissor o carrapato em períodos do ano de maior incidência de calor e umidade, tendo em vista o potencial zoonótico. A babesiose é causada pelos protozoários Babesia Canis e Babesia gibsoni transmitida pelo carrapato Rhipicephalus sanguineus, esses parasitas causam a hemólise das hemácias. Já a anaplasmose é oriunda de bactérias intracelulares Anaplasma platys ou Anaplasma phagocytophilum transmitidas pelo carrapato Rhipicephalus sanguineus, causando trombocitopenia. A rangeliose é ocasionada por um piroplasma Rangelia vitalli, transmitida por carrapatos Rhipicephalus sanguineus e Amblyomma aerolatum, se replicando nos eritrócitos. Objetivo: Relatar um caso clínico de uma canina. Relato de caso: canina castrada, sem raça definida, com 1 ano e dois meses, pesando 11,4 Kg, sendo atendida em uma clínica veterinária particular em Caxias do Sul/RS, apresentando apatia, hematoquezia, anorexia, prostração, epistaxe, sangramento em pontas de orelhas, mucosas hipocoradas com petéquias e febre. Durante o atendimento foi realizada coleta sanguínea para avaliação hematológica, esfregaço sanguíneo periférico da orelha, bioquímica sérica (creatinina, fosfatase alcalina, ureia, transaminase pirúvica) e urinálise, apresentando alterações, tais como anemia regenerativa, leucocitose por neutrofilia com desvio a esquerda, linfocitose, trombocitopenia, na urinálise houve presença de hemoglobina, leucócitos, proteinúria. No exame de esfregaço de sangue periférico apresentou-se negativo para hemoparasitas. O diagnóstico definitivo foi através da Reação em Cadeia da Polimerase (PCR), na qual evidenciou os agentes patogênicos de babesiose, rangeliose e anaplasmose. O tratamento instituído foi doxiciclina (5 mg/kg/q.12h durante 28 dias), azitromicina (10 mg/Kg/q.24h durante 10 dias), diaceturato de diminazeno (0,5ml/2Kg) repetindo em 14 dias a dose com prévia aplicação de atropina (0,044 mg/Kg), suplemento vitamínico mineral aminoácido (1 ml/q.24h). O animal se manteve bem clinicamente durante o tratamento, obtendo cura da enfermidade. Conclusão: Mesmo com o diagnóstico negativo de esfregaço sanguíneo periférico, com a suspeita clínica do animal foi solicitado PCR para confirmar a patologia, assim foi de suma importância a pesquisa de hemoparasitas pelos sinais clínicos do canino, juntamente do tratamento precoce e assertivo.

scholarly journals Clinical variations observed among the main hemoparasitosis caused by Rhipicephalus sanguineus in dogs

2021 ◽  
Vol 10 (12) ◽  
pp. e351101220680
Author(s):  
Felipe Arnaud Sampaio Alencar de Albuquerque ◽  
Thâmara Rossi Martins da Silva ◽  
Micael Siegert Schimmunech ◽  
Jaquelinne Andrade Dias ◽  
Paulo Henrique Gomes ◽  
...  
Keyword(s):  
Literature Review ◽  
Clinical Signs ◽  
Rhipicephalus Sanguineus ◽  
Diagnostic Techniques ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Babesia Gibsoni ◽  
Clinical Routine ◽  
Host Group ◽  
Different Types

Hemoparasitosis are diseases of great importance in the practice of a small animal’s veterinarian, due to their high number of cases in the clinical routine of these species, and the wide occurrence of their vectors. Related to hemoparasitosis in dogs, the tick Rhipicephalus sanguineus has the greatest importance due to parasitizing dogs, perpetuating hemoparasitosis in this host group. The present work describes a literature review about the three main hemoparasitosis in the routine of a veterinarian in Brazil, ehrlichiosis, caused by Ehrlichia canis, babesiosis caused by the protozoa Babesia canis and Babesia gibsoni, and anaplasmosis caused by Anaplasma platys, comparing them regarding the differences between clinical signs and laboratory alterations, such as the different types of anemia caused by these conditions that culminate in clinical signs related to them. It also aims to describe the similarity in the diagnostic techniques used and infection treatments, which in all cases is used tetracycline, the most commonly used is doxycycline, and highlight the need for further studies about this topic.

scholarly journals Hemoparasitas e bactérias hemotrópicas observadas por microscopia direta em amostras de sangue periférico de cães em uma clínica particular no município de Lorena, São Paulo, Brasil

2019 ◽  
Vol 17 (3) ◽  
pp. 16-20
Author(s):  
Luan Gavião Prado ◽  
Rafael Vieira Sene ◽  
Elisângela Camargo de Medeiros ◽  
Letícia Maioli do Nascimento ◽  
Ana Beatriz França de Faria
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Sao Paulo ◽  
Ehrlichia Canis ◽  
Babesia Canis ◽  
São Paulo ◽  
Anaplasma Platys

As hemoparasitoses, afecções responsáveis por complicações clínicas e hematológicas, são um grande desafio para o clínico e patologista clínico. Sua alta incidência em todo o território brasileiro e a dificuldade observada para o estabelecimento de um diagnóstico preciso levam muitas vezes ao emprego de tentativas e diagnóstico terapêutico. Os principais agentes etiológicos encontrados no Brasil são Anaplasma platys, Babesia canis vogeli, Ehrlichia canis, Mycoplasma haemocanis e, com menor frequência e importância clínica, Anaplasma phagocytophilum. Os altos índices pluviométricos e temperaturas elevadas, observados no Brasil, país tropical, favorecem os ciclos de vida dos artrópodes vetores de tais agentes, e tornam seu controle um grande desafio para veterinários e proprietários de animais. Os agentes etiológicos determinam manifestações clínicas inespecíficas: febre, apatia, anorexia e alterações hematológicas como trombocitopenia e anemia, o que pode dificultar ainda mais sua detecção. Este trabalho descreve a ocorrência de hematozoários em uma clínica veterinária particular no município de Lorena, São Paulo, e as principais alterações hematológicas encontradas nas avaliações dos animais acometidos pelas enfermidades.

scholarly journals Report of candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis canine natural infections in Massambaba restinga, Brazil

2019 ◽  
Author(s):  
Marcia Gonçalves Miranda ◽  
Bruno Alberigi ◽  
Flavya Mendes-de-Almeida ◽  
Alexandre José Rodrigues Bendas ◽  
Nadia Almosny ◽  
...  
Keyword(s):  
Climate Changes ◽  
Global Climate ◽  
The Other ◽  
Southeastern Brazil ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Babesia Gibsoni ◽  
Global Climate Changes ◽  
History Of ◽  
Healthy Dogs

Background: Tick-borne diseases are frequent in the Southeastern section of Brazil. The most prevalent canine parasites diagnosed are Ehrlichia canis, Babesia gibsoni, Babesia canis and Anaplasma platys, although Mycoplasma haemocanis and candidatus Mycoplasma haematoparvum have been registered in the country. Methods: When two clinically healthy dogs from a suburban area of the state of Rio de Janeiro, Brazil with history of being heavily infested with ticks were examined at Idexx Reference Laboratories, California for tick panel check. Results: One dog harbored DNA of candidatus Mycoplasma haematoparvum and the other DNA of Mycoplasma haemocanis. Conclusions: These results suggest that monitoring for tick infestations and tick-borne parasites must be permanent in southeastern Brazil, especially due to global climate changes which may contribute to spread and increase the number of infections in the Country.

scholarly journals Report of candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis canine natural infections in Massambaba restinga, Brazil

2019 ◽  
Author(s):  
Marcia Gonçalves Miranda ◽  
Bruno Alberigi ◽  
Flavya Mendes-de-Almeida ◽  
Alexandre José Rodrigues Bendas ◽  
Nadia Almosny ◽  
...  
Keyword(s):  
Climate Changes ◽  
Global Climate ◽  
The Other ◽  
Southeastern Brazil ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Babesia Gibsoni ◽  
Global Climate Changes ◽  
History Of ◽  
Healthy Dogs

Background: Tick-borne diseases are frequent in the Southeastern section of Brazil. The most prevalent canine parasites diagnosed are Ehrlichia canis, Babesia gibsoni, Babesia canis and Anaplasma platys, although Mycoplasma haemocanis and candidatus Mycoplasma haematoparvum have been registered in the country. Methods: When two clinically healthy dogs from a suburban area of the state of Rio de Janeiro, Brazil with history of being heavily infested with ticks were examined at Idexx Reference Laboratories, California for tick panel check. Results: One dog harbored DNA of candidatus Mycoplasma haematoparvum and the other DNA of Mycoplasma haemocanis. Conclusions: These results suggest that monitoring for tick infestations and tick-borne parasites must be permanent in southeastern Brazil, especially due to global climate changes which may contribute to spread and increase the number of infections in the Country.

Investigation of Tickborne Pathogens within Naturally Infected Brown Dog Tick (Ixodidae: Rhipicephalus Sanguineus) in Egypt by Light and Electron Microscopy

2020 ◽  
Keyword(s):  
Electron Microscopy ◽  
Salivary Gland ◽  
Light And Electron Microscopy ◽  
Rhipicephalus Sanguineus ◽  
Mammalian Host ◽  
Babesia Canis ◽  
Brown Dog Tick ◽  
Transmission Electron ◽  
Dog Tick ◽  
Theileria Spp

Tick borne pathogens present a significant health challenge to animals and human because a single tick may transmit multiple pathogens to a mammalian host during feeding. The present study detected tick-borne pathogens from pet dogs. A total of 666 ticks were collected from 144 pet and sheltered dogs in Egypt from April to September 2018. For hemolymph, midgut and salivary gland smears 546 ticks were used as well as 360 egg smears from 120 female tick were examined by light microscope. The infected ticks were prepared for transmission electron microscopy (TEM). Ticks were identified; Rhipicephalus sanguineus. Light microscopy showed infection rates of 44.69%, 68.50% & 15.75%, in hemolymph, midgut and salivary gland, respectively. H. canis recorded the highest rates in hemolymph and midgut (35.89% & 49.82%, respectively), but Theileria spp. was the lowest (0.73% & 2.93%, respectively). In salivary gland smears, Babesia canis. was detected in 13.55% and Theileria spp. in 1.83%. Mixed infection in same tick was recorded in 4.76% &0.37% in midgut and salivary gland smears, respectively. Babesia canis stages were recovered from 15% of egg smears. R. sanguineus was natural infected by Babesia, Theileria, Hepatozoon and Anaplasma phagocytophilum as well as mixed infections of protozoa accompanied by a complicated sign of diseases and failure in accurate diagnosis.

scholarly journals Efeitos de diferentes doses de hiclato de doxiciclina nos parâmetros hematológicos de cães com erliquiose

2016 ◽  
Vol 37 (1) ◽  
pp. 53
Author(s):  
Mariana Cristina Hoeppner Rondelli ◽  
Reinaldo Juan Garrido Palacios Junior ◽  
Igor Luiz Salardani Senhorello ◽  
Áureo Evangelista Santana ◽  
Mirela Tinucci-Costa
Keyword(s):  
Nested Pcr ◽  
Ehrlichia Canis ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Leptospira Spp

Resumo: Este estudo objetivou comparar os efeitos de duas doses de hiclato de doxiciclina sobre a contagem de hemácias, concentração de hemoglobina, hematócrito, contagem de leucócitos e plaquetas de cães com erliquiose. Foram estudados os grupos I, composto por cães saudáveis (n=6), negativos na sorologia para Ehrlichia canis e Leptospira spp., na PCR em tempo real para E. canis e Anaplasma platys e na semi–nested PCR para Babesia canis; grupos II (n=6) e III (n=6), compostos por cães com história clínica sugestiva, sorologia e/ou PCR em tempo real positivas para E. canis, e negativos na pesquisa de anticorpos anti-Leptospira spp., na PCR em tempo real para A. platys e na semi–nested PCR para B. canis. Os cães doentes foram tratados com hiclato de doxiciclina via oral a cada 12 horas por 30 dias (5 mg/kg, grupo II; 10 mg/kg, grupo III). Hemogramas foram feitos antes, decorridos 15 dias e aos 10 dias após o término dos tratamentos. Não houve diferenças entre os grupos nos tempos avaliados quanto às variáveis hemácias, hemoglobina, hematócrito e leucócitos. Observou-se diferença quanto aos trombócitos entre o grupo I e os grupos II e III (p<0,0001) no início do estudo. Aos 15 dias de tratamento, a média do grupo III foi menor que dos grupos I (p=0,008) e II (p=0,0007), indicando persistência da trombocitopenia, ausente no grupo II. Não houve diferença entre os grupos no tempo final, sugerindo que ambos os tratamentos promoveram aumento da contagem de plaquetas em cães naturalmente infectados por E. canis.

scholarly journals First Molecular Detection of Anaplasma platys and Coinfection with Babesia gibsoni in Dogs from Bengaluru, India

2020 ◽  
Vol 9 (3) ◽  
pp. 75-79
Author(s):  
Medha Karnik ◽  
Anjan Kumar ◽  
M. Manjula ◽  
H. D. Lohitha ◽  
R. Narendra ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Anaplasma Platys ◽  
Babesia Gibsoni

scholarly journals First report on molecular prevalence and identification of Anaplasma platys in dogs in Khon Kaen, Thailand

2021 ◽  
pp. 2613-2619
Author(s):  
Biethee Rani Sarker ◽  
Thongphet Mitpasa ◽  
Arayaporn Macotpet ◽  
Pattara-Anong Bupata ◽  
Somboon Sangmaneedet ◽  
...  
Keyword(s):  
Risk Factors ◽  
Platelet Count ◽  
Dna Sequencing ◽  
Similarity Index ◽  
Rhipicephalus Sanguineus ◽  
Severe Thrombocytopenia ◽  
Anaplasma Platys ◽  
Related Factors ◽  
Nested Polymerase Chain Reaction ◽  
Khon Kaen

Background and Aim: Anaplasma platys is a blood parasite that infects platelets, causing thrombocytopenia. Rhipicephalus sanguineus ticks are believed to transmit A. platys. To identify A. platys, nested polymerase chain reaction (PCR) has proven to be an effective diagnostic tool. In this study, the molecular prevalence of A. platys infection in dogs was investigated for the 1st time in the Khon Kaen region of Thailand. The association between risk factors and A. platys infection was also evaluated. Materials and Methods: A total of 130 blood samples were collected from dogs in Khon Kaen, Thailand. DNA from the samples was extracted and nested PCR was applied for molecular analysis. Platelet count and packed cell volume (PCV) levels were measured. Platelet counts were categorized into four grades: Non-thrombocytopenia (platelets >200,000 cells/μL), mild thrombocytopenia (platelets 150,000-200,000 cells/μL), moderate thrombocytopenia (platelets 100,000-150,000 cells/μL), and severe thrombocytopenia (platelets <100,000 cells/μL). Four categories for PCV levels of >37%, 30-37%, 20-29%, and <20% were defined as no anemia, mild anemia, moderate anemia, and severe anemia, respectively. DNA sequencing was analyzed using BTSeq™ (Barcode-Tagged Sequencing; CELEMICS, Seoul, South Korea) for similarity index. Results: Among the 130 samples, 9 (6.9%) were positive for A. platys infection. There was an association between low platelet count and infection (p<0.05). PCV level was also associated with A. platys infection (p<0.05). DNA sequencing results of the nine positive samples showed similarity to known sequences of A. platys with 99.36-100% nucleotide identity. These results suggested low genetic diversity in A. platys infecting dogs in the Khon Kaen area. Conclusion: By amplifying 16S rRNA, A. platys infection was detected in the blood of Thai dogs. Further work should be performed to identify risk factors potentially associated with A. platys infection in dogs in Khon Kaen. Other related factors should also be considered, such as location and breeding, as well as the environmental characteristics of each locality. In addition, sampling a larger number of animals may reveal predictors for the positivity of A. platys in dogs in this region.

scholarly journals Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

2015 ◽  
Vol 35 (5) ◽  
pp. 456-461 ◽  
Author(s):  
Andreina C. Araujo ◽  
Júlia A.G. Silveira ◽  
Sérgio S. Azevedo ◽  
Fernanda A. Nieri-Bastos ◽  
Múcio F.B. Ribeiro ◽  
...  
Keyword(s):  
Rural Areas ◽  
Direct Detection ◽  
Dna Amplification ◽  
Rhipicephalus Sanguineus ◽  
Immunofluorescence Assay ◽  
Semiarid Region ◽  
Babesia Canis ◽  
Logistic Regression Models ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Urban And Rural Areas

Abstract:This study aimed to report the prevalence of Babesia canis vogeli in dogs and ticks in the urban and rural areas of Petrolina, Pernambuco. Serum and peripheral blood samples of 404 dogs were tested by indirect immunofluorescence assay (IFA) and by blood smears, respectively. The presence of tick infestation was evaluated, and some specimens were submitted to DNA amplification by polymerase chain reaction (PCR). The presence of antibodies anti-B. canis vogeli was determinate in 57.9% (234/404) of dogs. The direct detection of Babesia spp was obtained in 0.5% (2/404) dogs by visualization of intraerythrocytic forms. Infestation by Rhipicephalus sanguineus sensu lato was observed in 54.5% (220/404) of dogs in both urban and rural areas. DNA of Babesia canis vogeli were obtained by PCR in 6% individual (3/50) and 8.7% of pool of ticks (7/80). The risk factors for the presence of anti-B. canis vogeli antibodies, as determined through the application of logistic regression models (P<0.05), were the following: medium breed size variables (P<0.001); contact with areas of forest (P=0.021); and access on the street (P=0.046). This study describes, for the first time, the confirmation of infection of B. canis vogeli in dogs and ticks in the semiarid region of Pernambuco, Brazil.

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