scholarly journals Utilização de extrato Artemisia vulgaris em hexano no controle in vitro de Rhipicephalus microplus

2021 ◽  
Vol 10 (13) ◽  
pp. e129101320759
Author(s):  
Thiago Luiz Pereira Marques ◽  
Gabriela Santos Freitas ◽  
Gabriela Barbosa Martins ◽  
Letícia Meirelles Ávila ◽  
Karoline Alves Machado ◽  
...  

As infestações por Rhiphicephalus microplus causam perdas significativas na bovinocultura brasileira provocando diversos prejuízos como, perda de peso e baixa conversão alimentar, podendo comprometer a produção de carne e leite. O extrato de plantas é utilizado como uma forma alternativa para o controle de carrapatos, visto que o uso indiscriminado de acaricidas está gerando populações de carrapatos cada vez mais resistentes. Assim, em conformidade com o exposto, o presente trabalho objetivou testar a eficiência reprodutiva in vitro de ativos extraídos em solvente apolar (hexano) da planta Artemisia vulgaris sobre o carrapato Rhipicephalus microplus. Inicialmente, para a avaliação das eficácias in vitro dos extratos da A. vulgaris, foram utilizadas teleóginas da colônia de R. microplus do Laboratório de Quimioterapia Experimental em Parasitologia Veterinária da UFRRJ (LQEPV), sendo separadas em grupos de 10 e submetidas ao teste de imersão em hexano na concentração de 1250 ppm, bem como controle positivo e negativo. A leitura do percentual de eclodibilidade que foi realizada para o cálculo da Eficiência Reprodutiva do Produto, foi feita através de análise de variância. Deste modo, ainda que hajam poucos trabalhos que comprovem a eficácia de A. vulgaris como um biocarrapaticida, pôde-se concluir com o presente estudo que, quando esta foi extraída em hexano demonstrou uma boa eficácia no controle in vitro de R. microplus.

2020 ◽  
Vol 9 (10) ◽  
pp. e5049108788
Author(s):  
Luciane Neris Cazella ◽  
Herika Line de Marko de Oliveira ◽  
Wanessa de Campos Bortolucci ◽  
Isabelle Luiz Rahal ◽  
Irinéia Paulina Baretta ◽  
...  

Baccharis dracunculifolia, native to Brazil and the main source of “green propolis”, has been reported with several biological activities, and may be a source of bovine tick control substituting synthetic acaricides. Objective: to evaluate the in vitro and ex situ acaricidal activity of B. dracunculifolia leaf and flower essential oils against Rhipicephalus microplus. Methodology: the essential oils were extracted by hydrodistillation and analyzed by a gas chromatography coupled to mass spectrometry; the acaricidal activity of the essential oil was evaluated in vitro against adult females and against the egg hatchability; moreover, the acaricidal activity against tick larvae was evaluated in vitro and ex situ. Results: the major class of the essential oils was oxygenated sesquiterpene (55.1% leaves 50.4% flowers) and the main compounds were (21.5% leaves; 20.6% flowers) and spathulenol (21.8% leaves; 20.3% flowers). The essential oil at 500 mg/mL was effective to control egg hatchability with a reduction of egg laying capacity and decrease of number of adult ticks and larvae. The larvicidal activity of the essential oil had LC99.9 from 35 to 37 mg/mL by probit analysis, and the essential oil from 11 to 14 mg/mL presented 85 to 95% of treatment efficiency in the ex situ test. Conclusion: B. dracunculifolia leaf and flower essential oils are stable and have application potential to control bovine ticks.


2019 ◽  
pp. 1951
Author(s):  
Dumar Jaramillo Hernández ◽  
Angélica González Reina ◽  
Natalia Pedraza Castillo ◽  
Jorge Iván Sierra Acevedo ◽  
Gina Lorena García Martínez ◽  
...  

Objetivo. Se evaluó la actividad acaricida de Momordica charantia (Mc), Megaskepasma erythrochlamys (Me) y Gliricidia sepium (Gs) sobre Rhipicephalus microplus (Rm). Materiales y métodos. Se realizó la marcha fitoquímica preliminar de hojas del extracto metanólico de Mc (EMc), del extracto etanólico de Me (EMe) y del extracto acetónico de Gs (EGs) a través de la técnica de colorimetría y cromatografía en capa delgada (CCD).  La actividad acaricida se realizó a través de pruebas in-vitro utilizando la prueba de inmersión de larvas  (LIT) y la prueba de inmersión de adultos (AIT). Para las pruebas in-situ se usaron bovinos en pastoreo infestados naturalmente con garrapatas, utilizando las CL50 obtenidas en las pruebas in-vitro AIT; posteriormente las teleoginas se llevaron  a incubación para evaluar su capacidad reproductiva. Resultados. Se determinó la presencia de varios grupos de metabolitos secundarios de interés acaricida. Se demostró el efecto acaricida de los extractos de las plantas sobre teleoginas; aunque sólo EGs mostró actividad larvicida. Los extractos a 160 mg/mL afectaron el ciclo de vida de Rm inhibiendo la ovoposición en un 46.9%, 66.1% y 84.03% (p<0.05) para EGs, EMc y EMe, respectivamente. Por otro lado, en las pruebas in situ se observó diferencia significativa (p<0.05) entre el tratamiento de EMc y EMe respecto a los grupos controles. Conclusiones. Los resultados obtenidos son prometedores para fortalecer la posibilidad de vinculación de los extractos de estas plantas dentro de planes integrados de control de garrapatas en sistemas de producción de bovinos.


2013 ◽  
Vol 192 (1-3) ◽  
pp. 296-300 ◽  
Author(s):  
Christian Cutullé ◽  
Léonore Lovis ◽  
Beatriz Inés D’Agostino ◽  
Gabriel Gerardo Balbiani ◽  
Gabriel Morici ◽  
...  

2018 ◽  
Vol 260 ◽  
pp. 1-4 ◽  
Author(s):  
Tatiane Novato ◽  
Geovany Amorim Gomes ◽  
Viviane Zeringóta ◽  
Cristiane Teixeira Franco ◽  
Débora Ramos de Oliveira ◽  
...  

2021 ◽  
Vol 12 (1) ◽  
pp. 101603
Author(s):  
O. Escobar-Chavarría ◽  
R. Cossío-Bayúgar ◽  
P. Ramírez-Noguera ◽  
M.G. Prado-Ochoa ◽  
A.M. Velázquez-Sánchez ◽  
...  

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
S. Parveen ◽  
R. Godara ◽  
R. Katoch ◽  
A. Yadav ◽  
P. K. Verma ◽  
...  

In vitro efficacy of ethanolic extracts obtained from the aerial parts ofAgeratum conyzoidesandArtemisia absinthiumwas assessed onRhipicephalus microplususing adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration forA. conyzoidesandA. absinthium, respectively. Acaricidal activity was found to be higher in the extract ofA. absinthiumwith LC50and LC95values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly(P<0.05)lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly(P<0.05). TheA. conyzoidesinhibited 90% hatching of eggs at the 20% concentration, whereasA. absinthiumshowed 100% inhibition at 5%, 10%, and 20% concentrations. The results show thatA. absinthiumhas better acaricidal properties thanA. conyzoidesand could be useful in controllingR. microplus.


2014 ◽  
Vol 113 (10) ◽  
pp. 3697-3701 ◽  
Author(s):  
K. N. C. Castro ◽  
D. F. Lima ◽  
L. C. Vasconcelos ◽  
J. R. S. A. Leite ◽  
R. C. Santos ◽  
...  

Author(s):  
S. Parafiniuk ◽  
E. Czerwiñska

The experiment evaluated the effect of plant extracts on germination and contamination of Vicia fabia seed “Bobas” variety of horse beans. The materials used for the studies were aqueous extracts made of 40 plant species. The studies aimed to indicate those plant species, which extracts limited their contamination, simultaneously having a stimulating impact on seeds germination. The germination capability of seeds stimulated extracts from green parts of Satureja hortensis, Zea mays stigmata, Verbascum thapsiforme flowers. The number of seeds colonised by microorganisms was limited by extracts from Zea mays stigmata, Taraxacum officinale roots, Urtica dioica leaves. The analysis of the germination capability of seeds and their contamination by germs showed that extracts prepared in the form of brew had the most favourable effect. All forms of extracts from green parts of Artemisia vulgaris,Taraxacum officinale roots,Urtica dioica leaves had the most favourable effect on germination of horse bean seeds and at the same time on limitation of their contamination.


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