Passive transfer of immunity against Cryptosporidium infection in neonatal mice using monoclonal antibodies

1993 ◽  
Vol 31 (3) ◽  
pp. 223
Author(s):  
M H Cho
Keyword(s):  
Monoclonal Antibodies ◽  
Passive Transfer ◽  
Cryptosporidium Infection ◽  
Neonatal Mice

Observations on the Resistance to Schistosome Infection of Neonatal Mice born to Immune Mothers with a Demonstration of Increased Resistance in Lactating Mice

1971 ◽  
Vol 45 (2-3) ◽  
pp. 223-228
Author(s):  
M. G. Taylor ◽  
D. A. Denham ◽  
G. S. Nelson
Keyword(s):  
Immune System ◽  
Incidental Finding ◽  
Virgin Female ◽  
Passive Transfer ◽  
Male Mice ◽  
Schistosome Infection ◽  
Female Mice ◽  
Neonatal Mice ◽  
Enhanced Resistance ◽  
Significant Difference

An attempt was made to demonstrate passive transfer of immunity using neonatal mice born to mothers resistant to schistosome infection. No immunity was demonstrated in the baby mice, suggesting that the classes of antibody which are active in this immune system are not transferrable neonatally.An interesting incidental finding was the apparent enhanced resistance of lactating mice compared to normal females or males and this was confirmed in another experiment. There was no significant difference between the susceptibility of normal females and male mice. More male worms were recovered from male mice than from virgin female mice.

scholarly journals Passive transfer of anti-laminin 5 antibodies induces subepidermal blisters in neonatal mice.

1996 ◽  
Vol 98 (7) ◽  
pp. 1509-1518 ◽  
Author(s):  
Z Lazarova ◽  
C Yee ◽  
T Darling ◽  
R A Briggaman ◽  
K B Yancey
Keyword(s):  
Passive Transfer ◽  
Laminin 5 ◽  
Neonatal Mice

Identification of Platelet Antigens by Monoclonal Antibodies Using Crossed Immunoelectrophoresis with Immunoblotting of the Monoclonal Antibody

1987 ◽  
Vol 57 (02) ◽  
pp. 212-216 ◽  
Author(s):  
L I Thorsen ◽  
G Gaudernack ◽  
F Brosstad ◽  
T M Pedersen ◽  
N O Solum
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Passive Transfer ◽  
Cellular Proteins ◽  
Nitrocellulose Membrane ◽  
Crossed Immunoelectrophoresis ◽  
Antigen Complex ◽  
Triton X ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA method is described for the identification of antigens by monoclonal antibodies. This is applicable whenever precipitating antibodies to the same antigens from a different species are available. The method is based upon: 1) Separation and immunoprécipitation of cellular proteins with a polyspecific antiserum in crossed immunoelectrophoresis in the presence of the non-denaturing detergent Triton X-100 and the monoclonal antibody. 2) Coprecipitation of the monoclonal antibody with its antigen. 3) Subsequent passive transfer of the monoclonal antibody in the antibody-antigen complex onto a nitrocellulose membrane. 4) Visualization of the blotted antibody using an enzyme-linked secondary antibody and a chromogenic substrate. 5) Identification of the corresponding antigen by comparisons to the immunoprecipitate pattern of the original immunoplate. To test this method we have analyzed the detection of the antigens recognized by six previously described monoclonal antibodies against platelet membrane proteins and von Willebrand factor. Specific immunoblots were obtained in each case using small amounts of monoclonal antibodies. Thus, the technique provides an alternative when epitopes are denatured by SDS, and avoids the use of radioactively labelled monoclonal antibodies.

Systemic passive transfer studies using IgM monoclonal antibodies to sulfatide

1995 ◽  
Vol 63 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Ettore Nardelli ◽  
Antonella Bassi ◽  
Giuseppe Mazzi ◽  
Patrizia Anzini ◽  
Nicola Rizzuto
Keyword(s):  
Monoclonal Antibodies ◽  
Passive Transfer

A mosquito AgTRIO monoclonal antibody reduces early Plasmodium infection of mice

2021 ◽  
Author(s):  
Yu-Min Chuang ◽  
Xu-Dong Tang ◽  
Erol Fikrig
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Protective Immunity ◽  
Passive Immunization ◽  
Passive Transfer ◽  
Infected Mosquito ◽  
Carboxyl Terminus ◽  
Plasmodium Infection ◽  
Significant Protection ◽  
Malaria Vaccines

Malaria begins when an infected mosquito injects saliva containing Plasmodium sporozoites into the skin of a vertebrate host. Passive immunization of mice with mosquito AgTRIO antisera offers significant protection against Plasmodium infection of mice. Furthermore, passive transfer of both AgTRIO antisera and an anti-circumsporozoite protein monoclonal antibody provides synergistic protection. In this study, we generated monoclonal antibodies against AgTRIO to delineate the regions of AgTRIO associated with protective immunity. Monoclonal antibody 13F-1 markedly reduced Plasmodium infection in mice and recognized a region, VDDLMAKFN, in the carboxyl terminus of AgTRIO. 13F-1 is an IgG2a isotype monoclonal antibody and the Fc region is required for protection. These data will aid in the generation of future malaria vaccines that may include both pathogen and vector antigens.

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