scholarly journals Characterization of Tambjamines Pigment from Marine Bacterium Pseudoalteromonas sp. PM2 Indigenous from Alor Island, Indonesia

2021 ◽  
Vol 3 (1) ◽  
pp. 16-23
Author(s):  
Edi Setiyono ◽  
Marcelinus Alfasisurya Setya Adhiwibawa ◽  
Matheus Randy Prabowo ◽  
Tatas H.P. Brotosudarmo
Keyword(s):  
16S Rrna ◽  
Marine Bacteria ◽  
Marine Bacterium ◽  
Rrna Gene ◽  
Accession Number ◽  
16S Rrna Gene Sequences ◽  
Different Types ◽  
New Finding ◽  
Yellow Pigments

Pigments from marine bacteria have attracted the attention for scientists because of their extensive applications and currently exploration of new pigment sources from marine bacteria is still ongoing. Recently, we have successfully isolated six new yellow-pigmented marine bacteria, strain PS2, PM2, SB11, SB13, SB21, and SB23, isolated from seawater from different sampling sites on Alor Island, Indonesia. The UV−Vis and FTIR spectra of the crude pigment extracts of the six strains showed the characteristics of tambjamines, a group of yellow pigments commonly found in nudibranchs and bryozoans. Moreover, separation and characterization of crude tambjamines extract resulted in five different types of tambjamine with maximum absorbance at the wavelength of 374−392 nm. Based on the analysis of 16S rRNA gene sequences, strain PM2 was closely related to several species in genus Pseudoalteromonas with a similarity of more than 99%. Strain PM2 was designed as Pseudoalteromonas sp. PM2 with accession number LC505058. So far, only two marine bacteria have been known to produce tambjamine and they are from genus Pseudoalteromonas. Our new finding indicated that in the group of marine bacteria, tambjamine might be only synthesized by members from genus Pseudoalteromonas. 

scholarly journals Molecular Characterization of anEndozoicomonas-Like Organism Causing Infection in the King Scallop (Pecten maximusL.)

2017 ◽  
Vol 84 (3) ◽  
Author(s):  
Irene Cano ◽  
Ronny van Aerle ◽  
Stuart Ross ◽  
David W. Verner-Jeffreys ◽  
Richard K. Paley ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Mass Mortality ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
The 16S Rrna Gene

ABSTRACTOne of the fastest growing fisheries in the UK is the king scallop (Pecten maximusL.), also currently rated as the second most valuable fishery. Mass mortality events in scallops have been reported worldwide, often with the causative agent(s) remaining uncharacterized. In May 2013 and 2014, two mass mortality events affecting king scallops were recorded in the Lyme Bay marine protected area (MPA) in Southwest England. Histopathological examination showed gill epithelial tissues infected with intracellular microcolonies (IMCs) of bacteria resemblingRickettsia-like organisms (RLOs), often with bacteria released in vascular spaces. Large colonies were associated with cellular and tissue disruption of the gills. Ultrastructural examination confirmed the intracellular location of these organisms in affected epithelial cells. The 16S rRNA gene sequences of the putative IMCs obtained from infected king scallop gill samples, collected from both mortality events, were identical and had a 99.4% identity to 16S rRNA gene sequences obtained from “CandidatusEndonucleobacter bathymodioli” and 95% withEndozoicomonasspecies.In situhybridization assays using 16S rRNA gene probes confirmed the presence of the sequenced IMC gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing, and bioinformatic analysis identified over 1,000 genes with high similarity to protein sequences fromEndozoicomonasspp. (ranging from 77 to 87% identity). Specific PCR assays were developed and applied to screen for the presence of IMC 16S rRNA gene sequences in king scallop gill tissues collected at the Lyme Bay MPA during 2015 and 2016. There was 100% prevalence of the IMCs in these gill tissues, and the 16S rRNA gene sequences identified were identical to the sequence found during the previous mortality event.IMPORTANCEMolluscan mass mortalities associated with IMCs have been reported worldwide for many years; however, apart from histological and ultrastructural characterization, characterization of the etiological agents is limited. In the present work, we provide detailed molecular characterization of anEndozoicomonas-like organism (ELO) associated with an important commercial scallop species.

scholarly journals Characterization of the GenusBifidobacteriumby Automated Ribotyping and 16S rRNA Gene Sequences

2006 ◽  
Vol 50 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Shinji Sakata ◽  
Chun Sun Ryu ◽  
Maki Kitahara ◽  
Mitsuo Sakamoto ◽  
Hidenori Hayashi ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Automated Ribotyping

scholarly journals Gaetbulibacter aestuarii sp. nov., isolated from shallow coastal seawater, and emended description of the genus Gaetbulibacter

2012 ◽  
Vol 62 (1) ◽  
pp. 150-154 ◽  
Author(s):  
Seong Chan Park ◽  
Han Na Choe ◽  
Keun Sik Baik ◽  
Kang Hyun Lee ◽  
Chi Nam Seong
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Bacterium ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Emended Description ◽  
South Sea

A rod-shaped, yellow and strictly aerobic marine bacterium, designated KYW382T, was isolated from seawater collected from the South Sea, Republic of Korea. Cells were Gram-negative and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and anteiso-C15 : 0. The DNA G+C content was 32.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW382T constituted an evolutionary lineage within the radiation enclosing the members of the genus Gaetbulibacter. The closest neighbour was Gaetbulibacter saemankumensis SMK-12T (96.1 % 16S rRNA gene sequence similarity). A number of phenotypic characteristics distinguished strain KYW382T from the described members of the genus Gaetbulibacter. On the basis of the data presented in this study, strain KYW382T represents a novel species, for which the name Gaetbulibacter aestuarii sp. nov. is proposed. The type strain is KYW382T ( = KCTC 23303T  = JCM 17455T). An emended description of the genus Gaetbulibacter is also given.

ARDRA and Identification of Intestinal Aerobic Bacteria from 4th Instar Larvae of Dendrolimu. kikuchii

2012 ◽  
Vol 518-523 ◽  
pp. 5523-5527
Author(s):  
Wu Xian Zhang ◽  
Jin Hua Wang ◽  
You He Sun ◽  
Biao Li ◽  
Zhi Xiong
Keyword(s):  
Genetic Diversity ◽  
Bacillus Subtilis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
16S Rdna ◽  
Enzyme Digestion ◽  
Aerobic Bacteria ◽  
Rrna Gene ◽  
Accession Number ◽  
16S Rrna Gene Sequences

Genetic diversity of 11 intestinal aerobic bacteria isolated from Dendrolimu. kikuchii was analysed, using PCR and ARDRA which used enzyme digestion of cloned 16S rRNA gene sequences. The results showed that 11 strains could be divided into 6 groups on 84% similarity level, it indicated that the intestinal aerobic bacteria genetic diversity was abundant. Sequencing the 6 representative strains’ 16S rDNA and submitting to GenBank, the accession number being JQ308104 to JQ308109 respectively. The 6 strains belonged to Klebsiella sp., Lysinibacillus sp., Brevibacillus sp., Bacillus subtilis, Gamma Proteobacterium and Brevibacillus limnophilus.

scholarly journals Characterization of the microbial community in different types of Daqu samples as revealed by 16S rRNA and 26S rRNA gene clone libraries

2014 ◽  
Vol 31 (1) ◽  
pp. 199-208 ◽  
Author(s):  
Xiao-Wei Zheng ◽  
Zheng Yan ◽  
M. J. Robert Nout ◽  
Teun Boekhout ◽  
Bei-Zhong Han ◽  
...  
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Gene Clone ◽  
Rrna Gene ◽  
Clone Libraries ◽  
Different Types ◽  
26S Rrna

scholarly journals Robiginitalea biformata gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae with a higher G+C content

2004 ◽  
Vol 54 (4) ◽  
pp. 1101-1106 ◽  
Author(s):  
Jang-Cheon Cho ◽  
Stephen J. Giovannoni
Keyword(s):  
Type Strain ◽  
Marine Bacterium ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Sargasso Sea ◽  
16S Rrna Gene Sequences ◽  
Novel Genus ◽  
The Family

Two Gram-negative, chemoheterotrophic, non-motile, rust-coloured, marine strains were isolated from the western Sargasso Sea by high-throughput culturing. Characterization of the two strains by polyphasic approaches indicated that they are members of the same species. Phylogenetic analyses based on 16S rRNA gene sequences using three treeing algorithms revealed that the strains formed a coherent and novel genus-level lineage within the family Flavobacteriaceae. The dominant fatty acids were branched or hydroxy acids, i15 : 0, i15 : 1 and 3-OH i17 : 0 being the most abundant. The higher DNA G+C content of the strains (55–56 mol%) clearly differentiated them from other genera of the family Flavobacteriaceae (27–44 mol%). It is proposed, from the polyphasic evidence, that the strains be placed into a novel genus and a novel species named Robiginitalea biformata gen. nov., sp. nov., with strain HTCC2501T (=ATCC BAA-864T=KCTC 12146T) as the type strain.

scholarly journals Detection and characterization of Pasteuria 16S rRNA gene sequences from nematodes and soils

2003 ◽  
Vol 53 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Y. P. Duan ◽  
H. F. Castro ◽  
T. E. Hewlett ◽  
J. H. White ◽  
A. V. Ogram
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences

scholarly journals Chromium Precipitation Activity and Molecular Characterization of Sulfate-Reducing Bacteria

2019 ◽  
Vol 4 (1) ◽  
pp. 15
Author(s):  
Dwiana Muflihah Yulianti ◽  
Endah Retnaningrum ◽  
Wahyu Wilopo
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sulfate Reducing Bacteria ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Sulfate Reducing ◽  
Sulfate Reducing Bioreactor ◽  
Reducing Bacteria

Chromium is one of the metals used in many areas of industry., However, chromium is toxic to organisms when present in large quantities in the environment. One of the method for treatment of hazardous waste containing chromium in the aquatic environment can be removed by bioremediation using sulfate-reducing bacteria (SRB). Therefore, the purpose of this research were to analyze the chromium precipitation activity of sulfate-reducing bacteria isolated from sulfate reducing bioreactor and its molecular identification using 16S rRNA gene sequences. The result observed that the isolate of sulfate-reducing bacteria (KGP1 strain) has chromium tolerancy ability up to 5 ppm. It also showed that the strain KGP1 could precipitate chromium up to 0.141 ppm (79 %) on 5 days incubation. Based on 16S rRNA gene sequences, this strain identified as Desulfovibrio aerotolerans.

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