The developmental changes in mitochondrial DNA content per cell in human cord blood leukocytes during gestation

The mitochondrial DNA (mtDNA) amount in cells as the basis for mitochondrial energy generating system, which produces ATP, plays an important role in the fetal development and postnatal morbidity. Isolated human cord blood leukocytes (HCBL) contribute very little to the overall metabolic turnover, but they may serve as easily available marker cells for the study of the mtDNA amount changes in cord blood during fetal development. The aim of our study was to analyze the mtDNA amount in HCBL. HCBL were isolated from cord blood samples of 107 neonates born between the 25th and 41st week of gestation. The mtDNA amount was analyzed by the real-time PCR method. The significant negative correlations were found between the relative mtDNA amount in HCBL and gestational age (r = -0.54, p<0.01) and birth weight (r = -0.43, p<0.01), respectively. The results revealed that the mtDNA content per cell decreases in HCBL with progressing fetal development. This may be explained by gradual shift of the hematopoiesis from fetal liver to bone marrow during the second half of pregnancy presumably accompanied by decreasing cell volume of HCBL as it was shown similarly in red blood cells.

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Developmental changes of mitochondrial DNA content and expression of genes involved in mtDNA transcription and maintenance in human fetal liver and muscle tissues

Biochimica et Biophysica Acta (BBA) - Bioenergetics ◽

10.1016/j.bbabio.2010.04.328 ◽

2010 ◽

Vol 1797 ◽

pp. 108-109 ◽

Cited By ~ 2

Author(s):

Martina Pejznochova ◽

Marketa Tesarova ◽

Hana Hansikova ◽

Martin Magner ◽

Tomas Honzik ◽

...

Keyword(s):

Mitochondrial Dna ◽

Dna Content ◽

Fetal Liver ◽

Developmental Changes ◽

Human Fetal Liver ◽

Expression Of Genes ◽

Muscle Tissues ◽

Mitochondrial Dna Content ◽

Mtdna Transcription

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Developmental variation in stem-cell markers from human fetal liver and umbilical cord blood leukocytes

Transfusion Medicine ◽

10.1046/j.1365-3148.1998.00134.x ◽

1998 ◽

Vol 8 (2) ◽

pp. 103-109 ◽

Cited By ~ 9

Author(s):

Kilpatrick ◽

Atkinson ◽

Palmer ◽

Murphy ◽

Turner

Keyword(s):

Stem Cell ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Fetal Liver ◽

Stem Cell Markers ◽

Blood Leukocytes ◽

Cell Markers ◽

Human Fetal Liver ◽

Developmental Variation

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Unique Differentiation Programs of Human Fetal Liver Stem Cells Shown Both In Vitro and In Vivo in NOD/SCID Mice

Blood ◽

10.1182/blood.v94.8.2686.420k15_2686_2695 ◽

1999 ◽

Vol 94 (8) ◽

pp. 2686-2695 ◽

Cited By ~ 42

Author(s):

Franck E. Nicolini ◽

Tessa L. Holyoake ◽

Johanne D. Cashman ◽

Pat P.Y. Chu ◽

Karen Lambie ◽

...

Keyword(s):

Cord Blood ◽

Fetal Liver ◽

Scid Mice ◽

Human Cord Blood ◽

Human Fetal Liver ◽

Human Erythropoietin ◽

Erythroid Precursors ◽

Lower Ratio

Comparative measurements of different types of hematopoietic progenitors present in human fetal liver, cord blood, and adult marrow showed a large (up to 250-fold), stage-specific, but lineage-unrestricted, amplification of the colony-forming cell (CFC) compartment in the fetal liver, with a higher ratio of all types of CFC to long-term culture-initiating cells (LTC-IC) and a lower ratio of total (mature) cells to CFC. Human fetal liver LTC-IC were also found to produce more CFC in LTC than cord blood or adult marrow LTC-IC, and more of the fetal liver LTC-IC–derived CFC were erythroid. Human fetal liver cells regenerated human multilineage hematopoiesis in NOD/SCID mice with the same kinetics as human cord blood and adult marrow cells, but sustained a high level of terminal erythropoiesis not seen in adult marrow-engrafted mice unless exogenous human erythropoietin (Epo) was injected. This may be due to a demonstrated 10-fold lower activity of murine versus human Epo on human cells, sufficient to distinguish between a differential Epo sensitivity of fetal and adult erythroid precursors. Examination of human LTC-IC, CFC, and erythroblasts generated either in NOD/SCID mice and/or in LTC showed the types of cells and hemoglobins produced also to reflect their ontological origin, regardless of the environment in which the erythroid precursors were generated. We suggest that ontogeny may affect the behavior of cells at many stages of hematopoietic cell differentiation through key changes in shared signaling pathways.

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