scholarly journals Mycelial Compatibility Groups and Microsatellite Markers Reveal Genetic Diversity Within and Among Populations of Sunflower Sclerotinia Sclerotiorum in China

2014 ◽  
Vol 3 (1) ◽  
pp. 21-31
Author(s):  
Yujie Wang ◽  
Yaguang Hou ◽  
Haoyu Bo ◽  
Hongyou Zhou ◽  
Lan Jing ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Microsatellite Markers ◽  
Sclerotinia Sclerotiorum ◽  
Inner Mongolia ◽  
Test Results ◽  
Marker Data ◽  
Microsatellite Haplotype ◽  
Mycelial Compatibility ◽  
Geographic Populations ◽  
Geographic Regions

The genetic variability and differentiation among 101 sunflower Sclerotinia sclerotiorum isolates collected from four different geographic regions of China were analyzed using mycelial compatibility groupings (MCGs) and microsatellite markers. Twenty three MCGs were identified among all tested isolates. The majority of isolates collected from the same region were grouped in to the same MCGs, indicating less genetic variation of S. sclerotiorum within the same region. But there still have exceptions for some isolates. Also microsatellite marker data revealed that all tested isolates from four geographic populations could be divided into three distinct clusters, isolates from Inner Mongolia and Ninxia regions formed cluster I, isolates from Heilongjiang and Xinjiang formed separate clusters II and III . The percentage of variance within and among different geographic populations was 84.54% and 15.46% respectively and both variances were significantly different from each other (p0.01). Meanwhile, association between the microsatellite haplotype and MCGs was observed but not so significant; majority isolates from the same MCG showed the same haplotype, but certain samples showed different haplotypes, although they belonged to the same MCG. Based on the virulence test results, we also found that MCGs not only represent the genetic variation of tested isolates, but also reflect their pathogenic ability to a certain extent.

The population of Sclerotinia sclerotiorum in Brazil is structured by mycelial compatibility groups

Plant Disease ◽  
2021 ◽  
Author(s):  
Rhaphael Alves Silva ◽  
Camila Geovana Ferro ◽  
Miller da Silva Lehner ◽  
Trazilbo José Paula Júnior ◽  
Eduardo S. G. Mizubuti
Keyword(s):  
Genetic Variation ◽  
Linkage Disequilibrium ◽  
Genetic Structure ◽  
Sclerotinia Sclerotiorum ◽  
Total Population ◽  
Random Mating ◽  
Genetic Groups ◽  
Close Relationship ◽  
Mycelial Compatibility ◽  
Variable Population

The genetic structure of the population of Sclerotinia sclerotiorum was analyzed using 238 individuals collected from different hosts. Individuals were characterized for microsatellite genotypes and mycelial compatibility groups (MCGs). A total of 22 MCGs and 64 multilocus lineages (MLLs) were identified. There was a close relationship between the MCGs and MLLs, but there was no association between MLLs and hosts or regions. At least 39 MCGs are present in Brazil and 68.5% of the isolates were assigned to either MCG 1 or 2. Eight new MCGs were found. Seven genetic groups were identified and associated with MCGs. Most genetic variation (70.0%) was due to differences among MCGs. High values of estimates of linkage disequilibrium among loci were more frequent in the total population (all MCGs). In contrast, there was evidence of random mating in subpopulations defined by MCGs 1 and 2. Additionally, there was evidence of outcrossing in the population of S. sclerotiorum in Brazil. The population was structured by MCGs, lineages originated from asexual reproduction or selfing prevail and are widely distributed in space, are persistent in time and affect many hosts, but there is evidence of some degree of outcrossing which may lead to a more genetically variable population in the future.

Microsatellite Markers Reveal Genetic Variation within Sclerotinia sclerotiorum Populations in Irrigated Dry Bean Crops in Brazil

2011 ◽  
Vol 159 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Eriston Vieira Gomes ◽  
Lucas Breseguello Do Nascimento ◽  
Marcos Augusto De Freitas ◽  
Luiz Carlos Bhering Nasser ◽  
Silvana Petrofeza
Keyword(s):  
Genetic Variation ◽  
Microsatellite Markers ◽  
Sclerotinia Sclerotiorum ◽  
Dry Bean

Genetic variation in three Chinese peat mosses (Sphagnum) based on microsatellite markers, with primer information and analysis of ascertainment bias

2008 ◽  
Vol 111 (2) ◽  
pp. 271-281 ◽  
Author(s):  
A. Jonathan Shaw ◽  
Tong Cao ◽  
Li-song Wang ◽  
Kjell Ivar Flatberg ◽  
Bergfrid Flatberg ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Microsatellite Markers ◽  
Ascertainment Bias

scholarly journals Genetic Variation of Sclerotinia sclerotiorum from Multiple Crops in the North Central United States

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0139188 ◽  
Author(s):  
Laura Aldrich-Wolfe ◽  
Steven Travers ◽  
Berlin D. Nelson
Keyword(s):  
United States ◽  
Genetic Variation ◽  
Sclerotinia Sclerotiorum ◽  
North Central ◽  
The North ◽  
Central United States

Genetic variation and structure of Sclerotinia sclerotiorum populations from soybean in Brazil

2018 ◽  
Vol 44 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Anthony Pannullo ◽  
Zhian N. Kamvar ◽  
Thomas J. J. Miorini ◽  
James R. Steadman ◽  
Sydney E. Everhart
Keyword(s):  
Genetic Variation ◽  
Sclerotinia Sclerotiorum

scholarly journals Genetic variation analysis of superior cotton varieties of Gossypium hirsutum through microsatellite markers

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Dede Nuraida ◽  
Yusuf Abdurrajak ◽  
Moh Amin ◽  
Utami S. Hastutik
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Gossypium Hirsutum ◽  
Microsatellite Markers ◽  
Size Range ◽  
Allele Frequencies ◽  
Variation Analysis ◽  
Ctab Method

This study was conducted in order to obtain information on genetic variation in populations rated as superior cotton (<em>Gossypium</em> <em>hirsutum</em> L.) varieties in Balittas Malang, Indonesia. The samples used 10 varieties of cotton Kanesia series and 2 other superior varieties that are LRA 5166 and ISA 205A. Indicators of genetic diversity are the number of alleles per <em>locus</em>, allele frequencies, and heterozygosity values. DNA was isolated from the leaves of 3- week-old seedlings using the CTAB method. Amplification was performed using 5 SSRs primer pairs of the JESPR series. The results showed five microsatellite <em>loci</em>, yielding 12 alleles with a size range of 80–500 bp, with an average number of alleles per <em>locus</em> of 4.60. The average values of heterozygosity of the five loci was high, at 0.71. Based on the number of alleles, allele frequencies and heterozygosity values, the genetic variation sampled in the superior cotton varieties studied here is quite high.

scholarly journals Carpogenic Germinability of Diverse Sclerotinia sclerotiorum Populations Within the Southwestern Australian Grain Belt

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 2891-2897
Author(s):  
Pippa J. Michael ◽  
King Yin Lui ◽  
Linda L. Thomson ◽  
Katia Stefanova ◽  
Sarita J. Bennett
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Temperature Fluctuations ◽  
Sclerotinia Stem Rot ◽  
Dark Cycle ◽  
Diurnal Temperature ◽  
Population Responses ◽  
Carpogenic Germination ◽  
Southwestern Australia ◽  
Temperature Regimes ◽  
Mycelial Compatibility

Sclerotinia stem rot, caused by the necrotrophic plant pathogen Sclerotinia sclerotiorum (Lib.) de Bary, is a major disease of canola and pulses in Australia. Current disease management relies greatly on cultural and chemical means of control. Timing of fungicide applications remains a challenge, because efficacy is dependent on accurate prediction of ascospore release and presence on the plant. The aims of this study were to determine the optimal temperature for carpogenic germination of S. sclerotiorum populations sampled from canola and lupin fields in southwestern Australia and characterize diversity using mycelial compatibility groupings (MCGs). Sclerotia were collected from four diseased canola and one diseased lupin field from across southwestern Australia. Forty sclerotia from each population were incubated at four alternating temperatures of 30/15, 20/15, 20/4, and 15/4°C (12-h/12-h light/dark cycle) and assessed every 2 to 3 days for a 180-day period. MCG groupings for populations were characterized using 12 reference isolates. Results indicated the time to initial carpogenic germination decreased as diurnal temperature fluctuations decreased, with a fluctuation of 5°C (20/15°C) having the most rapid initial germination followed by 11°C (15/4°C) followed by 16°C (20/4°C). Optimal germination temperature for all five populations was 20/15°C; however, population responses to other diurnal temperature regimes varied considerably. No germination was observed at 30/15°C. MCG results indicate extensive diversity within and between populations, with at least 40% of sclerotia within each population unable to be characterized. We suggest that this diversity has enabled S. sclerotiorum populations to adapt to varying environmental conditions within southwestern Australia.

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