Mycelial compatibility group and genetic variation of sunflower Sclerotinia sclerotiorum in Northeast China

2018 ◽  
Vol 102 ◽  
pp. 185-192
Author(s):  
Jia Liu ◽  
Qinglin Meng ◽  
Yunhua Zhang ◽  
Hongtao Xiang ◽  
Yichu Li ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Sclerotinia Sclerotiorum ◽  
Northeast China ◽  
Mycelial Compatibility ◽  
Compatibility Group

The population of Sclerotinia sclerotiorum in Brazil is structured by mycelial compatibility groups

Plant Disease ◽  
2021 ◽  
Author(s):  
Rhaphael Alves Silva ◽  
Camila Geovana Ferro ◽  
Miller da Silva Lehner ◽  
Trazilbo José Paula Júnior ◽  
Eduardo S. G. Mizubuti
Keyword(s):  
Genetic Variation ◽  
Linkage Disequilibrium ◽  
Genetic Structure ◽  
Sclerotinia Sclerotiorum ◽  
Total Population ◽  
Random Mating ◽  
Genetic Groups ◽  
Close Relationship ◽  
Mycelial Compatibility ◽  
Variable Population

The genetic structure of the population of Sclerotinia sclerotiorum was analyzed using 238 individuals collected from different hosts. Individuals were characterized for microsatellite genotypes and mycelial compatibility groups (MCGs). A total of 22 MCGs and 64 multilocus lineages (MLLs) were identified. There was a close relationship between the MCGs and MLLs, but there was no association between MLLs and hosts or regions. At least 39 MCGs are present in Brazil and 68.5% of the isolates were assigned to either MCG 1 or 2. Eight new MCGs were found. Seven genetic groups were identified and associated with MCGs. Most genetic variation (70.0%) was due to differences among MCGs. High values of estimates of linkage disequilibrium among loci were more frequent in the total population (all MCGs). In contrast, there was evidence of random mating in subpopulations defined by MCGs 1 and 2. Additionally, there was evidence of outcrossing in the population of S. sclerotiorum in Brazil. The population was structured by MCGs, lineages originated from asexual reproduction or selfing prevail and are widely distributed in space, are persistent in time and affect many hosts, but there is evidence of some degree of outcrossing which may lead to a more genetically variable population in the future.

scholarly journals Mycelial Compatibility Groups and Microsatellite Markers Reveal Genetic Diversity Within and Among Populations of Sunflower Sclerotinia Sclerotiorum in China

2014 ◽  
Vol 3 (1) ◽  
pp. 21-31
Author(s):  
Yujie Wang ◽  
Yaguang Hou ◽  
Haoyu Bo ◽  
Hongyou Zhou ◽  
Lan Jing ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Microsatellite Markers ◽  
Sclerotinia Sclerotiorum ◽  
Inner Mongolia ◽  
Test Results ◽  
Marker Data ◽  
Microsatellite Haplotype ◽  
Mycelial Compatibility ◽  
Geographic Populations ◽  
Geographic Regions

The genetic variability and differentiation among 101 sunflower Sclerotinia sclerotiorum isolates collected from four different geographic regions of China were analyzed using mycelial compatibility groupings (MCGs) and microsatellite markers. Twenty three MCGs were identified among all tested isolates. The majority of isolates collected from the same region were grouped in to the same MCGs, indicating less genetic variation of S. sclerotiorum within the same region. But there still have exceptions for some isolates. Also microsatellite marker data revealed that all tested isolates from four geographic populations could be divided into three distinct clusters, isolates from Inner Mongolia and Ninxia regions formed cluster I, isolates from Heilongjiang and Xinjiang formed separate clusters II and III . The percentage of variance within and among different geographic populations was 84.54% and 15.46% respectively and both variances were significantly different from each other (p0.01). Meanwhile, association between the microsatellite haplotype and MCGs was observed but not so significant; majority isolates from the same MCG showed the same haplotype, but certain samples showed different haplotypes, although they belonged to the same MCG. Based on the virulence test results, we also found that MCGs not only represent the genetic variation of tested isolates, but also reflect their pathogenic ability to a certain extent.

scholarly journals Telomere and microsatellite primers reveal diversity among Sclerotinia sclerotiorum isolates from Brazil

2002 ◽  
Vol 27 (2) ◽  
pp. 211-215 ◽  
Author(s):  
LYNDEL W. MEINHARDT ◽  
NELSON A. WULFF ◽  
CLÁUDIA M. BELLATO ◽  
SIU M. TSAI
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Ribosomal Subunit ◽  
Rflp Analysis ◽  
Dna Amplification ◽  
Genotypic Differences ◽  
Microsatellite Primers ◽  
Telomere Sequence ◽  
Center Pivot ◽  
Mycelial Compatibility ◽  
Compatibility Group

Sclerotinia sclerotiorum, the causal agent of white mold, is a problem of winter bean (Phaseolus vulgaris) production in Brazil under center-pivot irrigation. Isolates of S. sclerotiorum were obtained from a center-pivot-irrigated field near Guaíra-SP, Brazil. Mycelial compatibility group (MCG) studies revealed the presence of only two MCG. PCR/RFLP analysis of the ITS1-5.8S-ITS2 ribosomal subunit regions of these field isolates of S. sclerotiorum failed to show any genetic differences between these two MCGs. DNA amplification with a chromosomal telomere sequence-based primer and one microsatellite primer revealed genetic polymorphisms among isolates within the same MCG. Isolates taken from beans and two other crops from another region of Brazil showed the same two MCG and had identical banding patterns for the telomere and microsatellite primers. These findings support the use of telomere sequence-based primers for revealing genotypic differences among S. sclerotiorum isolates.

scholarly journals Genetic Variation of Sclerotinia sclerotiorum from Multiple Crops in the North Central United States

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0139188 ◽  
Author(s):  
Laura Aldrich-Wolfe ◽  
Steven Travers ◽  
Berlin D. Nelson
Keyword(s):  
United States ◽  
Genetic Variation ◽  
Sclerotinia Sclerotiorum ◽  
North Central ◽  
The North ◽  
Central United States

Genetic variation and structure of Sclerotinia sclerotiorum populations from soybean in Brazil

2018 ◽  
Vol 44 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Anthony Pannullo ◽  
Zhian N. Kamvar ◽  
Thomas J. J. Miorini ◽  
James R. Steadman ◽  
Sydney E. Everhart
Keyword(s):  
Genetic Variation ◽  
Sclerotinia Sclerotiorum

scholarly journals Carpogenic Germinability of Diverse Sclerotinia sclerotiorum Populations Within the Southwestern Australian Grain Belt

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 2891-2897
Author(s):  
Pippa J. Michael ◽  
King Yin Lui ◽  
Linda L. Thomson ◽  
Katia Stefanova ◽  
Sarita J. Bennett
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Temperature Fluctuations ◽  
Sclerotinia Stem Rot ◽  
Dark Cycle ◽  
Diurnal Temperature ◽  
Population Responses ◽  
Carpogenic Germination ◽  
Southwestern Australia ◽  
Temperature Regimes ◽  
Mycelial Compatibility

Sclerotinia stem rot, caused by the necrotrophic plant pathogen Sclerotinia sclerotiorum (Lib.) de Bary, is a major disease of canola and pulses in Australia. Current disease management relies greatly on cultural and chemical means of control. Timing of fungicide applications remains a challenge, because efficacy is dependent on accurate prediction of ascospore release and presence on the plant. The aims of this study were to determine the optimal temperature for carpogenic germination of S. sclerotiorum populations sampled from canola and lupin fields in southwestern Australia and characterize diversity using mycelial compatibility groupings (MCGs). Sclerotia were collected from four diseased canola and one diseased lupin field from across southwestern Australia. Forty sclerotia from each population were incubated at four alternating temperatures of 30/15, 20/15, 20/4, and 15/4°C (12-h/12-h light/dark cycle) and assessed every 2 to 3 days for a 180-day period. MCG groupings for populations were characterized using 12 reference isolates. Results indicated the time to initial carpogenic germination decreased as diurnal temperature fluctuations decreased, with a fluctuation of 5°C (20/15°C) having the most rapid initial germination followed by 11°C (15/4°C) followed by 16°C (20/4°C). Optimal germination temperature for all five populations was 20/15°C; however, population responses to other diurnal temperature regimes varied considerably. No germination was observed at 30/15°C. MCG results indicate extensive diversity within and between populations, with at least 40% of sclerotia within each population unable to be characterized. We suggest that this diversity has enabled S. sclerotiorum populations to adapt to varying environmental conditions within southwestern Australia.

scholarly journals Effect of some fungicides against the growth inhibition of Sclerotinia sclerotiorum mycelial compatibility groups

2015 ◽  
Vol 55 (4) ◽  
pp. 354-361 ◽  
Author(s):  
Alireza Dalili ◽  
Saeed Bakhtiari ◽  
Hossein Barari ◽  
Majid Aldaghi
Keyword(s):  
Growth Inhibition ◽  
Sclerotinia Sclerotiorum ◽  
Integrated Management ◽  
In Vitro Tests ◽  
Sclerotinia Stem Rot ◽  
Mazandaran Province ◽  
Brassica Napus L ◽  
Northern Iran ◽  
Mycelial Compatibility

Abstract Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of Sclerotinia stem rot, is one of the most important pathogens of Brassica napus L. in northern Iran. In this study, 13 mycelial compatibility groups (MCGs) of the fungus were identified among 31 isolates sampled from four regions of Mazandaran province, Iran. Effective fungicides are useful in the integrated management of the disease. So, the effect of tebuconazole, propiconazole, cyproconazole, and Rovral-TS at five doses (0.0001, 0.001, 0.01, 0.1, and 1 ppm) was studied on the growth inhibition of S. sclerotiorum as in vitro tests. Maximum inhibition (100%) of S. sclerotiorum mycelial growth was obtained by the highest dose (1 ppm) of all tested fungicides, as well as by the doses of 0.1 and 0.01 ppm of propiconazole, cyproconazole, and tebuconazole. In this investigation, the reaction of S. sclerotiorum isolates belonging to different MCGs was evaluated against tebuconazole, propiconazole, cyproconazole, and Rovral-TS at their EC50 ranges. The results revealed that there was high variation of S. sclerotiorum MCGs against different fungicides. The inhibition percentage varied between 4.29% and 71.72%.

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