scholarly journals Histone Demethylase PHF8 Is Required for the Development of the Zebrafish Inner Ear and Posterior Lateral Line

2020 ◽  
Vol 8 ◽  
Author(s):  
Jing He ◽  
Zhiwei Zheng ◽  
Xianyang Luo ◽  
Yongjun Hong ◽  
Wenling Su ◽  
...  
Keyword(s):  
Cell Migration ◽  
Inner Ear ◽  
Lateral Line ◽  
Target Genes ◽  
Histone Demethylase ◽  
Otic Vesicle ◽  
Lateral Line System ◽  
Potential Candidate ◽  
Line System ◽  
Posterior Lateral Line

Histone demethylase PHF8 is crucial for multiple developmental processes, and hence, the awareness of its function in developing auditory organs needs to be increased. Using in situ hybridization (ISH) labeling, the mRNA expression of PHF8 in the zebrafish lateral line system and otic vesicle was monitored. The knockdown of PHF8 by morpholino significantly disrupted the development of the posterior lateral line system, which impacted cell migration and decreased the number of lateral line neuromasts. The knockdown of PHF8 also resulted in severe malformation of the semicircular canal and otoliths in terms of size, quantity, and position during the inner ear development. The loss of function of PHF8 also induced a defective differentiation in sensory hair cells in both lateral line neuromasts and the inner ear. ISH analysis of embryos that lacked PHF8 showed alterations in the expression of many target genes of several signaling pathways concerning cell migration and deposition, including the Wnt and FGF pathways. In summary, the current findings established PHF8 as a novel epigenetic element in developing auditory organs, rendering it a potential candidate for hearing loss therapy.

scholarly journals Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Akihiro Urasaki ◽  
Seiya Morishita ◽  
Kosuke Naka ◽  
Minato Uozumi ◽  
Kouki Abe ◽  
...  
Keyword(s):  
Cell Migration ◽  
Lateral Line ◽  
Lateral Line System ◽  
Collective Cell Migration ◽  
Line System ◽  
Posterior Lateral Line

scholarly journals Development of the posterior lateral line system in Thunnus thynnus, the atlantic blue-fin tuna, and in its close relative Sarda sarda

2010 ◽  
Vol 54 (8-9) ◽  
pp. 1317-1322 ◽  
Author(s):  
Alain Ghysen ◽  
Kevin Schuster ◽  
Denis Coves ◽  
Fernando de la Gandara ◽  
Nikos Papandroulakis ◽  
...  
Keyword(s):  
Lateral Line ◽  
Close Relative ◽  
Lateral Line System ◽  
Thunnus Thynnus ◽  
Line System ◽  
Posterior Lateral Line ◽  
Sarda Sarda

Electroreceptors and Direction Specific Arrangement in the Lateral Line System of Salamanders?

1981 ◽  
Vol 36 (5-6) ◽  
pp. 493-496 ◽  
Author(s):  
Bernd Fritzsch
Keyword(s):  
Lateral Line ◽  
Fiber Bundles ◽  
Sensory Cells ◽  
Lateral Line System ◽  
Line System ◽  
Lateral Line Organ ◽  
Posterior Lateral Line ◽  
Afferent Fibers ◽  
Lateral Line Afferents ◽  
Line Organ

Abstract The arrangement of the lateral line afferents of salamanders as revealed by transganglionic staining with horse­ radish peroxidase is described. Each lateral line organ is supplied by two fibers only. In the medulla these two afferent fibers run in separate fiber bundles. It is suggested, that only those fibers contacting lateral line sensory cells with the same polarity form together one bundle. Bundles formed by anterior or posterior lateral line afferents are also clearly separated. Beside the lateral line organs smaller pit organs are described. These organs are supplied by one afferent only which reveals an arrangement in the medulla different from that of the lateral line afferents. Based on anatomical facts, these small pit organs are considered to be electroreceptors. Centrifugally projecting neurons, most probably efferents, are described in the medulla.

Labeling Second-Order Sensory Neurons in the Posterior Lateral-Line System of Zebrafish

2013 ◽  
Vol 2013 (12) ◽  
pp. pdb.prot079475-pdb.prot079475
Author(s):  
K. Schuster ◽  
A. Ghysen
Keyword(s):  
Sensory Neurons ◽  
Lateral Line ◽  
Second Order ◽  
Lateral Line System ◽  
Line System ◽  
Posterior Lateral Line

The zebrafish homologs of SET/I2PP2A oncoprotein: expression patterns and insights into their physiological roles during development

2016 ◽  
Vol 473 (24) ◽  
pp. 4609-4627 ◽  
Author(s):  
Iliana Serifi ◽  
Eleni Tzima ◽  
Katerina Soupsana ◽  
Zoe Karetsou ◽  
Dimitris Beis ◽  
...  
Keyword(s):  
Amino Acids ◽  
Lateral Line ◽  
Gene Networks ◽  
Expression Patterns ◽  
Otic Vesicle ◽  
Lateral Line System ◽  
Loss Of Function ◽  
Line System ◽  
Eye Retina

The oncoprotein SET/I2PP2A (protein phosphatase 2A inhibitor 2) participates in various cellular mechanisms such as transcription, cell cycle regulation and cell migration. SET is also an inhibitor of the serine/threonine phosphatase PP2A, which is involved in the regulation of cell homeostasis. In zebrafish, there are two paralogous set genes that encode Seta (269 amino acids) and Setb (275 amino acids) proteins which share 94% identity. We show here that seta and setb are similarly expressed in the eye, the otic vesicle, the brain and the lateral line system, as indicated by in situ hybridization labeling. Whole-mount immunofluorescence analysis revealed the expression of Seta/b proteins in the eye retina, the olfactory pit and the lateral line neuromasts. Loss-of-function studies using antisense morpholino oligonucleotides targeting both seta and setb genes (MOab) resulted in increased apoptosis, reduced cell proliferation and morphological defects. The morphant phenotypes were partially rescued when MOab was co-injected with human SET mRNA. Knockdown of setb with a transcription-blocking morpholino oligonucleotide (MOb) resulted in phenotypic defects comparable with those induced by setb gRNA (guide RNA)/Cas9 [CRISPR (clustered regularly interspaced short palindromic repeats)-associated 9] injections. In vivo labeling of hair cells showed a significantly decreased number of neuromasts in MOab-, MOb- and gRNA/Cas9-injected embryos. Microarray analysis of MOab morphant transcriptome revealed differential expression in gene networks controlling transcription in the sensory organs, including the eye retina, the ear and the lateral line. Collectively, our results suggest that seta and setb are required during embryogenesis and play roles in the zebrafish sensory system development.

scholarly journals Cell Proliferation and Collective Cell Migration During Zebrafish Lateral Line System Development Are Regulated by Ncam/Fgf-Receptor Interactions

2021 ◽  
Vol 8 ◽  
Author(s):  
Ramona Dries ◽  
Annemarie Lange ◽  
Sebastian Heiny ◽  
Katja I. Berghaus ◽  
Martin Bastmeyer ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Lateral Line ◽  
System Development ◽  
Lateral Line System ◽  
Collective Cell Migration ◽  
Fgf Receptor ◽  
Line System ◽  
Posterior Lateral Line ◽  
Neural Cell Adhesion ◽  
Reduce Cell Proliferation

The posterior lateral line system (pLLS) of aquatic animals comprises small clustered mechanosensory organs along the side of the animal. They develop from proneuromasts, which are deposited from a migratory primordium on its way to the tip of the tail. We here show, that the Neural Cell Adhesion Molecule Ncam1b is an integral part of the pathways initiating and regulating the development of the pLLS in zebrafish. We find that morpholino-knockdowns of ncam1b (i) reduce cell proliferation within the primordium, (ii) reduce the expression of Fgf target gene erm, (iii) severely affect proneuromast formation, and (iv) affect primordium migration. Ncam1b directly interacts with Fgf receptor Fgfr1a, and a knockdown of fgfr1a causes similar phenotypic changes as observed in ncam1b-morphants. We conclude that Ncam1b is involved in activating proliferation by triggering the expression of erm. In addition, we demonstrate that Ncam1b is required for the expression of chemokine receptor Cxcr7b, which is crucial for directed primordial migration. Finally, we show that the knockdown of ncam1b destabilizes proneuromasts, suggesting a further function of Ncam1b in strengthening the cohesion of proneuromast cells.

scholarly journals The Inner Ear is Responsible for Detection of Infrasound in the Perch (Perca Fluviatilis)

1992 ◽  
Vol 171 (1) ◽  
pp. 163-172 ◽  
Author(s):  
HANSERIK KARLSEN
Keyword(s):  
Inner Ear ◽  
Lateral Line ◽  
Low Frequency ◽  
Perca Fluviatilis ◽  
Lateral Line System ◽  
Threshold Values ◽  
Line System ◽  
Frequency Detection ◽  
Perch Perca Fluviatilis ◽  
Cardiac Conditioning

In a previous study of infrasound detection in the cod, the inner ear was suggested to be the sensory organ responsible for the responses. However, a possible involvement of the lateral-line system in the observed low-frequency detection could not be ruled out. The infrasound sensitivity was therefore studied in perch (Perca fluviatilis) with normal and blocked lateral-line organs. The experiments were performed using a standing wave acoustic tube and the cardiac conditioning technique. All perch readily responded to infrasound frequencies down to 0.3 Hz with threshold values of approximately 2×10−4 ms−2. These thresholds were not affected by complete blocking of the lateral-line system with Co2+, which suggests that the inner ear is responsible for the observed infrasound detection by the perch.

Ultrastructural effects of cisplatin on the inner ear and lateral line system of zebrafish (Danio rerio) larvae

2011 ◽  
Vol 32 (4) ◽  
pp. 293-299 ◽  
Author(s):  
Luisa Giari ◽  
Bahram Sayyaf Dezfuli ◽  
Laura Astolfi ◽  
Alessandro Martini
Keyword(s):  
Inner Ear ◽  
Lateral Line ◽  
Danio Rerio ◽  
Lateral Line System ◽  
Line System
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