scholarly journals “It Takes Two to Tango”: Role of Neglected Macrophage Manipulators Coronin 1 and Protein Kinase G in Mycobacterial Pathogenesis

2020 ◽  
Vol 10 ◽  
Author(s):  
Saradindu Saha ◽  
Payel Das ◽  
Somdeb BoseDasgupta
Keyword(s):  
Protein Kinase ◽  
Protein Kinase G ◽  
Mycobacterial Pathogenesis

scholarly journals H 2 O 2 -Induced Dilation in Human Coronary Arterioles: Role of Protein Kinase G Dimerization and Large-Conductance Ca 2+ -Activated K + Channel Activation

2012 ◽  
Vol 110 (3) ◽  
pp. 471-480 ◽  
Author(s):  
David X. Zhang ◽  
Lena Borbouse ◽  
Debebe Gebremedhin ◽  
Suelhem A. Mendoza ◽  
Natalya S. Zinkevich ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase G ◽  
K Channel ◽  
Channel Activation ◽  
Coronary Arterioles

scholarly journals Critical role of protein kinase G in the long-term balance between defensive and appetitive behaviors induced by aversive stimuli in Aplysia

2020 ◽  
Vol 383 ◽  
pp. 112504
Author(s):  
Ruma Chatterji ◽  
Sarah Khoury ◽  
Emanuel Salas ◽  
Marcy L. Wainwright ◽  
Riccardo Mozzachiodi
Keyword(s):  
Protein Kinase ◽  
Critical Role ◽  
Protein Kinase G ◽  
Aversive Stimuli ◽  
Appetitive Behaviors

scholarly journals Role of Protein Kinase G in Growth and Glutamine Metabolism of Mycobacterium bovis BCG

2005 ◽  
Vol 187 (16) ◽  
pp. 5852-5856 ◽  
Author(s):  
Liem Nguyen ◽  
Anne Walburger ◽  
Edith Houben ◽  
Anil Koul ◽  
Stefan Muller ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Mycobacterium Bovis ◽  
Glutamine Metabolism ◽  
Protein Kinase G ◽  
In Vitro Growth ◽  
Wild Type ◽  
Putative Operon ◽  
Glutamine Uptake

ABSTRACT The survival of pathogenic mycobacteria in macrophages requires the eukaryotic enzyme-like serine/threonine protein kinase G. This kinase with unknown specificity is secreted into the cytosol of infected macrophages and inhibits phagosome-lysosome fusion. The pknG gene is the terminal gene in a putative operon containing glnH, encoding a protein potentially involved in glutamine uptake. Here, we report that the deletion of pknG did not affect either glutamine uptake or intracellular glutamine concentrations. In vitro growth of Mycobacterium bovis BCG lacking pknG was identical to that of the wild type. We conclude that in M. bovis BCG, glutamine metabolism is not regulated by protein kinase G.

scholarly journals Role of Protein Kinase G in Growth and Glutamine Metabolism of Mycobacterium bovis BCG

2005 ◽  
Vol 187 (20) ◽  
pp. 7165-7165
Author(s):  
Liem Nguyen ◽  
Anne Walburger ◽  
Edith Houben ◽  
Anil Koul ◽  
Stefan Muller ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Mycobacterium Bovis ◽  
Glutamine Metabolism ◽  
Protein Kinase G ◽  
Mycobacterium Bovis Bcg

scholarly journals Role of protein kinase G in nitric oxide- and cGMP-induced relaxation of newborn ovine pulmonary veins

1999 ◽  
Vol 87 (3) ◽  
pp. 993-998 ◽  
Author(s):  
Yuansheng Gao ◽  
Srinivas Dhanakoti ◽  
Jean-Francois Tolsa ◽  
J. Usha Raj
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Biochemical Study ◽  
Critical Role ◽  
Pulmonary Veins ◽  
Protein Kinase G ◽  
Fourth Generation ◽  
Cgmp Analog ◽  
A Cell

In a variety of systemic blood vessels, protein kinase G (PKG) plays a critical role in mediating relaxation induced by agents that elevate cGMP, such as nitric oxide. The role of PKG in nitric oxide- and cGMP-induced relaxation is less certain in the pulmonary circulation. In the present study, we examined the effects of inhibitors of PKG on the responses of isolated fourth-generation pulmonary veins of newborn lambs (10 ± 1 days of age) to nitric oxide and cGMP. In vessels preconstricted with endothelin-1, nitric oxide and 8-bromo-cGMP (a cell-membrane-permeable cGMP analog) induced concentration-dependent relaxation. The relaxation was significantly attenuated by β-phenyl-1, N 2-etheno-8-bromoguanosine-3′,5′-cyclic monophosphorothionate (Rp-8-Br-PET-cGMPS; a PKG inhibitor) and N-[2-(methylamino)ethyl]5-isoquinolinesulfonamide [H-8; an inhibitor of PKG and protein kinase A (PKA)] but was not affected by KT-5720 (a PKA inhibitor). Biochemical study showed that PKG activity in newborn ovine pulmonary veins was inhibited by 8-Br-PET-cGMPS and H-8 but not by KT-5720. PKA activity was not affected by 8-Br-PET-cGMPS but was inhibited by H-8 and KT-5720. These results suggest that PKG is involved in relaxation of pulmonary veins of newborn lambs induced by nitric oxide and cGMP.

Role of Protein Kinase G in Nitric Oxide Deficiency-induced Supersensitivity to Nitrovasodilator in Rat Pulmonary Artery

2008 ◽  
Vol 51 (5) ◽  
pp. 450-456 ◽  
Author(s):  
Praveen K Gupta ◽  
Jaganathan Subramani ◽  
Thakur Uttam Singh ◽  
Marie Dennis Marcus Leo ◽  
Anurag S Sikarwar ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Pulmonary Artery ◽  
Protein Kinase G ◽  
Nitric Oxide Deficiency

scholarly journals Role of phosphodiesterase and protein kinase G on nitric oxide-induced inhibition of prolactin release from the rat anterior pituitary

2000 ◽  
pp. 279-284 ◽  
Author(s):  
MO Velardez ◽  
A De Laurentiis ◽  
M del Carmen Diaz ◽  
M Lasaga ◽  
D Pisera ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Anterior Pituitary ◽  
Inhibitory Effect ◽  
Male Rats ◽  
Protein Kinase G ◽  
Camp Concentration ◽  
Prolactin Release ◽  
Pituitary Glands

OBJECTIVE: In order to determine the mechanism by which nitric oxide (NO) inhibits prolactin release, we investigated the participation of cGMP-dependent cAMP-phosphodiesterases (PDEs) and protein kinase G (PKG) in this effect of NO. METHODS: Anterior pituitary glands of male rats were incubated with inhibitors of PDE and PKG with or without sodium nitroprusside (NP). Prolactin release, and cAMP and cGMP concentrations were determined by RIA. RESULTS AND CONCLUSIONS: The inhibitory effect of NP (0.5 mmol/l) on prolactin release and cAMP concentration was blocked by EHNA (10(-4)mol/l) and HL-725 (10(-4)mol/l), inhibitors of cGMP-stimulated cAMP-PDE (PDE2). 8-Br-cGMP (10(-4) and 10(-3)mol/l), which mimics cGMP as a mediator of NP effects on prolactin release, also decreased cAMP concentration. Zaprinast (10(-4)mol/l), a selective inhibitor of specific cGMP-PDE (PDE5), potentiated the NP effect on cAMP concentration. Rp-8-[(4-chlorophenyl)thio]-cGMP triethylamine (Rp-8-cGMP, 10(-7)-10(-6)mol/l), an inhibitor of PKG, reversed the effect of NP on prolactin release. The present study suggests that several mechanisms are involved in the inhibitory effect of NO on prolactin release. The activation of PDE2 by cGMP may mediate the inhibitory effect of NO on cAMP concentration and therefore on prolactin release. NO-activated PKG may also be participating in the inhibitory effect of NO on prolactin release.

Endothelial cell protein kinase G inhibits release of EDHF through a PKG-sensitive cation channel

2001 ◽  
Vol 280 (3) ◽  
pp. H1272-H1277 ◽  
Author(s):  
K. A. Dora ◽  
C. J. Garland ◽  
H. Y. Kwan ◽  
X. Yao
Keyword(s):  
Endothelial Cells ◽  
Protein Kinase ◽  
Vascular Endothelial Cells ◽  
Cation Channel ◽  
Mesenteric Arteries ◽  
Protein Kinase G ◽  
Cell Protein ◽  
Vascular Endothelial ◽  
Inside Out

The release of dilator agents from vascular endothelial cells is modulated by changes in cytosolic Ca2+ concentration ([Ca2+]i). In this study, we demonstrate the presence of a Ca2+-permeable cation channel in inside-out membrane patches of endothelial cells isolated from small mesenteric arteries. The activity of the channel is increased by KT-5823, a highly selective inhibitor of protein kinase G (PKG), while it is decreased by direct application of active PKG. Application of KT-5823 induces Ca2+ influx in the endothelial cells isolated from small mesenteric arteries, and it also causes endothelium-dependent relaxations in isolated small mesenteric arteries. KT-5823-induced relaxations in small mesenteric arteries are greatly reduced by 35 mM K+ or 50 nM charybdotoxin + 50 nM apamin, suggesting that endothelium-derived hyperpolarizing factor (EDHF) is the participating dilator. The involvement of EDHF is further supported by experiments in which the relaxations of small mesenteric arteries are shown to be accompanied by membrane repolarization. These data strongly argue for a major role of a PKG-sensitive cation channel in modulating the release of EDHF from endothelial cells in rat small mesenteric arteries.

scholarly journals Capacitation and Ca2+influx in spermatozoa: role of CNG channels and protein kinase G

Andrology ◽  
2013 ◽  
Vol 2 (1) ◽  
pp. 145-154 ◽  
Author(s):  
A. Cisneros-Mejorado ◽  
L. Hernández-Soberanis ◽  
M. C. Islas-Carbajal ◽  
D. Sánchez
Keyword(s):  
Protein Kinase ◽  
Protein Kinase G
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