scholarly journals Editorial: Progress in Pathogen Identification Based on Mass Spectrometry

2022 ◽  
Vol 11 ◽  
Author(s):  
Junping Peng ◽  
Yi-Wei Tang ◽  
Di Xiao
Keyword(s):  
Mass Spectrometry ◽  
Pathogen Identification

Rapid Pathogen Identification With Direct Application of MALDI-TOF Mass Spectrometry on an Endophthalmitis Vitreous Sample Without Prior Culture

2019 ◽  
Vol 3 (4) ◽  
pp. 255-259 ◽  
Author(s):  
Lindsay Y. Chun ◽  
Laura Dolle Molle ◽  
Olaf Schneewind ◽  
Dominique Missiakas ◽  
Kathleen G. Beavis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Direct Analysis ◽  
Causative Organism ◽  
Pathogen Identification ◽  
Maldi Tof Ms ◽  
Hand Motion ◽  
Maldi Tof ◽  
Microbiological Methods ◽  
Vitreous Sample ◽  
Tof Ms

Purpose:We report a case of a 72-year-old man with bleb-related endophthalmitis (BRE) whose vitreous samples were directly analyzed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapidly identify the causative organism, whereas the results from conventional microbiological techniques were negative.Methods:We analyzed BRE vitreous samples with MALDI-TOF MS (Vitek MS, bioMérieux) for rapid pathogen identification without prior culture. Samples were also analyzed with standard microbiological methods.Results:Within 1 hour of sample acquisition, MALDI-TOF MS identified Gemella sanguinis from the undiluted vitreous sample from vitrectomy without prior culture with a confidence value of 99.7%. Gram stain and cultures from aqueous and vitreous samples were negative for 28 days after acquisition. The patient’s right-eye vision improved from hand motion to 20/50 2 months later.Conclusions:Our findings suggest that the direct analysis of intraocular samples with MALDI-TOF MS could be a novel, promising adjuvant method of rapid endophthalmitis diagnosis.

scholarly journals Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Accelerates Pathogen Identification and May Confer Benefit in the Outcome of Peritoneal Dialysis-Related Peritonitis

2016 ◽  
Vol 54 (5) ◽  
pp. 1381-1383 ◽  
Author(s):  
Wei-Hung Lin ◽  
Jyh-Chang Hwang ◽  
Chin-Chung Tseng ◽  
Yu-Tzu Chang ◽  
An-Bang Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Pathogen Identification ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorganisms earlier (average time saved, 64 h for all pathogens), and patients had a shorter hospital stay (mean ± standard deviation, 5.2 ± 4.8 days versus 8.2 ± 4.5 days,P= 0.001).

scholarly journals 2146. MALDI-TOF Mass Spectrometry Rapid Pathogen Identification and Outcomes of Patients with Bloodstream Infection: A Systematic Review and Meta-analysis

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S727-S727
Author(s):  
Ronan Hsieh ◽  
Rania Mekary ◽  
Raymund Li ◽  
Chi-Yang Lin ◽  
Tzu-Hua Weng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bloodstream Infection ◽  
Clinical Outcomes ◽  
Publication Bias ◽  
Meta Analysis ◽  
Pathogen Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Meta Regression ◽  
Tof Ms

Abstract Background Several studies showed inconsistent results on the efficiency measures of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology and patients’ clinical outcomes. A meta-analysis was conducted to determine the effectiveness of MALDI-TOF MS-based bacteriology in improving the accuracy of microbiology report and clinical outcomes. Methods PubMed and EMBASE databases were searched from database inception through May 1, 2018 for pre–post and parallel comparative studies that evaluated the use of MALDI-TOF MS for identification of microorganism from blood culture. Pooled effect estimates were derived using the random-effects model. Univariate meta-regression on trial-level covariates was used to assess heterogeneity sources. Funnel plot, Begg’s and Egger’s tests were used to assess publication bias. Results Thirteen studies with 3,534 patients were meta-analyzed. Compared with conventional methods, MALDI-TOF MS was associated with 34% reduction in mortality (RR = 0.66; 95% CI: 0.54; 0.81; I2 = 27.6%; 9 studies); 5.3-hour reduction in time-to-effective antibiotic therapy (95% CI: -6.4; -4.3; I2 = 98.0%), 24.5-hour reduction in time to identify bacteria (95% CI: −25.7; −23.3; I2 = 91.0%); 0.9-day reduction in hospital stay (95% CI: −1.4; −0.3; I2 = 56.6%), and US$4100 saving in direct hospitalization cost (95% CI: $−8,200; $−113; I2 = 66.1%). No significant heterogeneity sources were found (all P-interaction from meta-regression > 0.05) and no statistical evidence for publication bias was found (all P > 0.05). Conclusion Rapid pathogen identification by MALDI-TOF MS with or without antibiotic stewardship was associated with reduced mortality, improved outcomes of bloodstream infection, and may be cost-effective among patients with bloodstream infection. Nevertheless, a multicenter randomized controlled trial is needed to confirm findings of these pre–post comparison studies. Disclosures All authors: No reported disclosures.

scholarly journals Bacillus pumilus severe wound infection in a healthy ten years old child: a rare case report

2019 ◽  
Vol 7 (8) ◽  
pp. 3195
Author(s):  
Jari Intra ◽  
Cecilia Sarto ◽  
Massimo Andreotti ◽  
Achille Marino ◽  
Tiziana Varisco ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Case Report ◽  
Wound Infection ◽  
Rare Case ◽  
Bacillus Pumilus ◽  
Left Knee ◽  
Pathogen Identification ◽  
Environmental Surfaces ◽  
Rare Case Report ◽  
Patient’S Outcome

Bacillus pumilus is commonly isolated from a wide variety of soils, plants and environmental surfaces, but rarely from human specimens. In this report, we describe a case of infection caused by B. pumilus in a healthy 10-years-old child. The microorganism was recovered from a severe wound of the left knee after three days from trauma. Pathogen identification was carried out by mass spectrometry. The patient's outcome was positive following an ampicillin/sulbactam treatment without complications.

scholarly journals Work Flow Analysis of Around-the-clock Processing of Blood Culture Samples and Integrated MALDI-TOF Mass Spectrometry Analysis for the Diagnosis of Bloodstream Infections

2013 ◽  
Vol 59 (11) ◽  
pp. 1649-1656 ◽  
Author(s):  
Wilhelm Schneiderhan ◽  
Alexander Grundt ◽  
Stefan Wörner ◽  
Peter Findeisen ◽  
Michael Neumaier
Keyword(s):  
Mass Spectrometry ◽  
Blood Culture ◽  
Real Time ◽  
Work Flow ◽  
Pathogen Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

BACKGROUND Because sepsis has a high mortality rate, rapid microbiological diagnosis is required to enable efficient therapy. The effectiveness of MALDI-TOF mass spectrometry (MALDI-TOF MS) analysis in reducing turnaround times (TATs) for blood culture (BC) pathogen identification when available in a 24-h hospital setting has not been determined. METHODS On the basis of data from a total number of 912 positive BCs collected within 140 consecutive days and work flow analyses of laboratory diagnostics, we evaluated different models to assess the TATs for batch-wise and for immediate response (real-time) MALDI-TOF MS pathogen identification of positive BC results during the night shifts. The results were compared to TATs from routine BC processing and biochemical identification performed during regular working hours. RESULTS Continuous BC incubation together with batch-wise MALDI-TOF MS analysis enabled significant reductions of up to 58.7 h in the mean TATs for the reporting of the bacterial species. The TAT of batch-wise MALDI-TOF MS analysis was inferior by a mean of 4.9 h when compared to the model of the immediate work flow under ideal conditions with no constraints in staff availability. CONCLUSIONS Together with continuous cultivation of BC, the 24-h availability of MALDI-TOF MS can reduce the TAT for microbial pathogen identification within a routine clinical laboratory setting. Batch-wise testing of positive BC loses a few hours compared to real-time identification but is still far superior to classical BC processing. Larger prospective studies are required to evaluate the contribution of rapid around-the-clock pathogen identification to medical decision-making for septicemic patients.

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