scholarly journals Eucalyptus Plantation Age and Species Govern Soil Fungal Community Structure and Function Under a Tropical Monsoon Climate in China

2021 ◽  
Vol 2 ◽  
Author(s):  
Bing Liu ◽  
Zhaolei Qu ◽  
Yang Ma ◽  
Jie Xu ◽  
Pei Chen ◽  
...  
Keyword(s):  
Community Structure ◽  
Potential Function ◽  
Fungal Community ◽  
Fungal Communities ◽  
Monsoon Climate ◽  
Fungal Community Structure ◽  
Plantation Age ◽  
Soil Fungal Community ◽  
Different Ages ◽  
Tropical Monsoon

Fungi perform crucial roles in nutrient cycles, but there is limited information on how soil fungal communities vary with stand age and tree species. Eucalyptus has been extensively planted in China, which has caused severe soil erosion and water deficiency due to short rotation management. In this study, the fungal community structure and potential function in Eucalyptus plantations with different ages (1–5+ years) and species (Eucalyptus urophylla × Eucalyptus grandis, Eucalyptus camaldulens, and Eucalyptus pellita) under a tropical monsoon climate in China were characterized by Illumina Miseq coupled with FUNGuild analysis. The results showed that the fungal alpha diversity decreased with an increase in the age of the plantation. Plantations of different ages and species formed distinct fungal communities and potential functional structures, respectively (p < 0.05), in which the age of the plantation contributed more to the variations. At high taxonomic levels, the soil fungal community changed from the dominance of orders belonging to Ascomycota (Pleosporales, Chaetothyriales, and Eurotiales) to orders belonging to Basidiomycota (Agaricales, Sebacinales, Cantharellales, and Russulales) with increasing plantation age. The community potential function shifted from the dominance of plant pathogens to a higher abundance of saprotrophs and symbiotrophs. The organic carbon of the soil was the key environmental driver to both the fungal community and potential functional structure. The results provide useful information on the importance of fungi for the management of Eucalyptus plantations.

scholarly journals Comparison of Soil Fungal Community Structure in Different Peanut Rotation Sequences Using Ribosomal Intergenic Spacer Analysis in Relation to Aflatoxin-Producing Fungi

2011 ◽  
Vol 101 (1) ◽  
pp. 52-57 ◽  
Author(s):  
H. Sudini ◽  
C. R. Arias ◽  
M. R. Liles ◽  
K. L. Bowen ◽  
R. N. Huettel
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
Intergenic Spacer ◽  
Fungal Communities ◽  
Special Focus ◽  
Specific Primers ◽  
Relative Population ◽  
Fungal Community Structure ◽  
Ribosomal Intergenic Spacer ◽  
Soil Fungal Community

The present study focuses on determining soil fungal community structure in different peanut-cropping sequences by using a high-resolution DNA fingerprinting technique: ribosomal intergenic spacer analysis (RISA). This study was initiated to determine fungal community profiles in four peanut-cropping sequences (continuous peanut, 4 years of continuous bahiagrass followed by peanut, peanut-corn-cotton, and peanut-cotton rotations), with a special focus to evaluate whether the profiles under investigation may have also indicated microbial differences that could affect Aspergillus flavus populations. Results indicated 75% similarities among fungal communities from the same cropping sequences as well as with similar times of sampling. Polymerase chain reaction (PCR)-based detection of A. flavus directly from these soils was carried out using A. flavus-specific primers (FLA1 and FLA2) and also through quantitative estimation on A. flavus and A. parasiticus agar medium. Population levels of A. flavus in soil samples ranged from zero to 1.2 × 103 CFU g–1 of soil (based on culturable methods); however, the fungus was not detected with A. flavus-specific primers. The minimum threshold limit at which these aflatoxin-producing fungi could be detected from the total soil genomic DNA was determined through artificial inoculation of samples with 10-fold increases in concentrations. The results indicated that a minimum population density of 2.6 × 106 CFU g–1 of soil is required for PCR detection in our conditions. These results are useful in further determining the relative population levels of these fungi in peanut soils with other soil fungi. This is a new approach to understanding soil fungal communities and how they might change over time and under different rotation systems.

scholarly journals Soil Fungal Communities and Enzyme Activities along Local Tree Species Diversity Gradient in Subtropical Evergreen Forest

Forests ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1321
Author(s):  
Ziqi Fu ◽  
Qin Chen ◽  
Pifeng Lei ◽  
Wenhua Xiang ◽  
Shuai Ouyang ◽  
...  
Keyword(s):  
Community Structure ◽  
Acid Phosphatase ◽  
Species Diversity ◽  
Fungal Community ◽  
Tree Species ◽  
Fungal Communities ◽  
Tree Species Diversity ◽  
Fungal Community Structure ◽  
Soil Fungal Community ◽  
Soil Acid Phosphatase

The majority of studies have found that an increase in tree species diversity can increase the productivity of forest stands thanks to complimentary effects with enhanced resource use efficiency or selection effects; however, it is unclear how tree species diversity affects the soil fungal community and enzyme activities in subtropical evergreen forests. In this study, we used soil high-throughput sequencing to investigate the soil fungal community structure and diversity in the central area of tree clusters in the gradient of tree species richness formed by four possible dominant tree species (Pinus massoniana Lamb., Choerospondias axillaris Roxb., Cyclobalanopsis glauca Thunb. and Lithocarpus glaber Thunb.) in subtropical evergreen broad-leaved forest. The results showed that soil organic carbon content and total nitrogen content were significantly higher in mixed tree clusters, and that soil fungal richness and diversity increased with the increase in tree species diversity (1–3 species). Soil acid phosphatase and urease activity were also enhanced with tree species diversity (p < 0.05). The relative abundance of soil symbiotic fungi (ectomycorrhizal fungi) decreased, while the relative abundance of saprotrophic fungi increased. Redundancy analysis (RDA) revealed that soil acid phosphatase activity was the main factor affecting soil fungal communities and functional guilds, and that soil water content was the main driving force behind fungal trophic modes. In subtropical forests, changes in tree species diversity have altered the soil fungal community structure and trophic modes and functions, accelerating the decomposition of organic matter, increasing nutrient cycling, and perhaps also changing the nutrient absorption of trees.

scholarly journals Seasonal variations in soil fungal communities and co-occurrence networks along an altitudinal gradient in the cold temperate zone of China: A case study on Oakley Mountain

2020 ◽  
Author(s):  
Li Ji ◽  
Yan Zhang ◽  
Yuchun Yang ◽  
Lixue Yang
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
Altitudinal Gradient ◽  
Soil Fungi ◽  
Clustering Coefficient ◽  
Fungal Communities ◽  
High Latitudes ◽  
Fungal Community Structure ◽  
Soil Fungal Community ◽  
Relative Abundances

AbstractThe biogeography of soil fungi has attracted much attention in recent years; however, studies on this topic have mainly focused on mid- and low-altitude regions. The seasonal patterns of soil fungal community structure and diversity along altitudinal gradients under the unique climatic conditions at high latitudes remain unclear, which limits our insight into soil microbial interactions and the mechanisms of community assembly. In this study, Illumina MiSeq sequencing was used to investigate the spatiotemporal changes in soil fungal communities along an altitudinal gradient (from 750 m to 1420 m) on Oakley Mountain in the northern Greater Khingan Mountains. Altitude had significant impacts on the relative abundances of the dominant phyla and classes of soil fungi, and the interaction of altitude and season significantly affected the relative abundances of Ascomycota and Basidiomycota. The number of soil fungal taxa and Faith’s phylogenetic diversity (PD) index tended to monotonically decline with increasing elevation. Soil moisture (SM), soil temperature (ST) and pH were the main factors affecting fungal community structure in May, July and September, respectively. The soil dissolved organic carbon (DOC) content significantly shaped the soil fungal community composition along the altitudinal gradient throughout the growing season. Compared to that in May and July, the soil fungal network in September had more nodes and links, a higher average degree and a higher average clustering coefficient. The nine module nodes in the co-occurrence network were all Ascomycota taxa, and the identities of the keystone taxa of soil fungi in the network showed obvious seasonality. Our results demonstrated that altitude has stronger effects than season on soil fungal community structure and diversity at high latitudes. In addition, the co-occurrence network of soil fungi exhibited obvious seasonal succession, which indicated that the keystone taxa of soil fungi exhibit niche differentiation among seasons.

scholarly journals Water-Covered Depth with the Freeze–Thaw Cycle Influences Fungal Communities on Rice Straw Decomposition

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1113
Author(s):  
Xiaolong Lin ◽  
Zongmu Yao ◽  
Xinguang Wang ◽  
Shangqi Xu ◽  
Chunjie Tian ◽  
...  
Keyword(s):  
Community Structure ◽  
Rice Straw ◽  
Fungal Community ◽  
Fungal Communities ◽  
Straw Decomposition ◽  
Freeze Thaw ◽  
Fungal Community Structure ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Rice Straw Decomposition

Rice is a staple food for the world’s population. However, the straw produced by rice cultivation is not used sufficiently. Returning rice straw to the field is an effective way to help reduce labor and protect the soil. This study focused on the effect of water-covered depth with the freeze–thaw cycle on rice straw decomposition and the soil fungal community structure in a field in Northeast China. The field and controlled experiments were designed, and the fungal ITS1 region was tested by high-throughput sequencing for analyzing the fungal communities in this study. The results showed that water coverage with the freeze–thaw cycle promoted the decomposition of rice straw and influenced the fungal community structure; by analyzing the network of the fungal communities, it was found that the potential keystone taxa were Penicillium, Talaromyces, Fusarium, and Aspergillus in straw decomposition; and the strains with high beta-glucosidase, carboxymethyl cellulase, laccase, lignin peroxidase, and manganese peroxidase could also be isolated in the treated experiment. Furthermore, plant pathogenic fungi were found to decrease in the water-covered treatment. We hope that our results can help in rice production and straw return in practice.

scholarly journals Uncovering fungal community composition in natural habitat of Ophiocordyceps sinensis using high-throughput sequencing and culture-dependent approaches

2020 ◽  
Author(s):  
Chuanbo Zhang ◽  
Chao-Hui Ren ◽  
Yan-Li Wang ◽  
Qi-Qi Wang ◽  
Yun-Sheng Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
High Throughput ◽  
Fungal Community ◽  
High Throughput Sequencing ◽  
Fungal Communities ◽  
Bioactive Metabolites ◽  
Fungal Community Structure ◽  
Ophiocordyceps Sinensis ◽  
Culturable Fungi ◽  
Culture Dependent

Abstract Background The fungal communities inhabiting natural Ophiocordyceps sinensis play critical ecological roles in alpine meadow ecosystem, contribute to infect host insect, influence the occurrence of O. sinensis, and are repertoire of potential novel metabolites discovery. However, a comprehensive understanding of fungal communities of O. sinensis remain elusive. Therefore, the present study aimed to unravel fungal communities of natural O. sinensis using combination of high-throughput sequencing and culture-dependent approach. Results A total of 280,519 high-quality sequences, belonging to 5 fungal phyla, 15 classes, 41 orders, 79 families, 112 genera, and 352 putative operational taxonomic units (OTUs) were obtained from natural O. sinensis using high-throughput sequencing. Among of which, 43 genera were identified in external mycelial cortices (EMC), Ophiocordyceps, Sebacinia, Archaeorhizomyces were predominant genera with the abundance of 95.86%, 1.14%, 0.85%, respectively. Total 66 genera were identified from soil microhabitat, Inocybe, Archaeorhizomyces, Unclassified Thelephoraceae, Tomentella, Thelephora, Sebacina, Unclassified Ascomycota, Unclassified Fungi were predominant genera with an average abundance of 53.32%, 8.69%, 8.12%, 8.12%, 7.21%, 4.6%, 3.08% and 3.05%, respectively. The fungal communities in external mycelial cortices (EMC) were significantly distinct from the soil microhabitat (Soil). Meanwhile, seven culture media that benefit for the growth of O. sinensis were used to isolate culturable fungi at 16 °C, resulted in 77 fungal strains isolated for rDNA ITS sequence analysis, belonging to 33 genera, including Ophiocordyceps, Trichoderma, Cytospora, Truncatella, Dactylonectria, Isaria, Cephalosporium, Fusarium, Cosmospora, Paecilomyces, etc.. Among all culturable fungi, Mortierella and Trichoderma were predominant genera of total isolates. Conclusions The significantly distinction and overlap in fungal community structure between two approaches highlight that integration of approaches would generate more information than either of them. Our finding is the first investigation of fungal community structure of natural O. sinensis by two approachs, provide new insight into O. sinensis associated fungi, and support that microbiota of O. sinensis is an untapped source for novel bioactive metabolites discovery.

scholarly journals Soil Physicochemical Properties and the Rhizosphere Soil Fungal Community in a Mulberry (Morus alba L.)/Alfalfa (Medicago sativa L.) Intercropping System

Forests ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 167 ◽  
Author(s):  
Mengmeng Zhang ◽  
Ning Wang ◽  
Jingyun Zhang ◽  
Yanbo Hu ◽  
Dunjiang Cai ◽  
...  
Keyword(s):  
Community Structure ◽  
Environmental Factors ◽  
Physicochemical Properties ◽  
Fungal Community ◽  
Total Carbon ◽  
Soil Physicochemical Properties ◽  
Planting Pattern ◽  
Fungal Community Structure ◽  
Soil Fungal Community ◽  
Soil Environmental Factors

A better understanding of soil fungal communities is very useful in revealing the effects of an agroforestry system and would also help us to understand the fungi-mediated effects of agricultural practices on the processes of soil nutrient cycling and crop productivity. Compared to conventional monoculture farming, agroforestry systems have obvious advantages in improving land use efficiency and maintaining soil physicochemical properties, reducing losses of water, soil material, organic matter, and nutrients, as well as ensuring the stability of yields. In this study, we attempted to investigate the impact of a mulberry/alfalfa intercropping system on the soil physicochemical properties and the rhizosphere fungal characteristics (such as the diversity and structure of the fungal community), and to analyze possible correlations among the planting pattern, the soil physicochemical factors, and the fungal community structure. In the intercropping and monoculture systems, we determined the soil physicochemical properties using chemical analysis and the fungal community structure with MiSeq sequencing of the fungal ITS1 region. The results showed that intercropping significantly improved the soil physicochemical properties of alfalfa (total nitrogen, alkaline hydrolysable nitrogen, available potassium, and total carbon contents). Sequencing results showed that the dominant taxonomic groups were Ascomycota, Basidiomycota, and Mucoromycota. Intercropping increased the fungal richness of mulberry and alfalfa rhizosphere soils and improved the fungal diversity of mulberry. The diversity and structure of the fungal community were predominantly influenced by both the planting pattern and soil environmental factors (total nitrogen, total phosphate, and total carbon). Variance partitioning analysis showed that the planting pattern explained 25.9% of the variation of the fungal community structure, and soil environmental factors explained 63.1% of the variation. Planting patterns and soil physicochemical properties conjointly resulted in changes of the soil fungal community structure in proportion.

Warming and disturbance alter soil microbiome diversity and function in a northern forest ecotone

2020 ◽  
Vol 96 (7) ◽  
Author(s):  
Michael E Van Nuland ◽  
Dylan P Smith ◽  
Jennifer M Bhatnagar ◽  
Artur Stefanski ◽  
Sarah E Hobbie ◽  
...  
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
Fungal Communities ◽  
Soil Microbiome ◽  
Northern Forest ◽  
Fungal Community Structure ◽  
Open Canopy ◽  
Ground Warming ◽  
And Function ◽  
Forest Ecotone

ABSTRACT The response to global change by soil microbes is set to affect important ecosystem processes. These impacts could be most immediate in transitional zones, such as the temperate-boreal forest ecotone, yet previous work in these forests has primarily focused on specific subsets of microbial taxa. Here, we examined how bacterial and fungal communities respond to simulated above- and below-ground warming under realistic field conditions in closed and open canopy treatments in Minnesota, USA. Our results show that warming and canopy disturbance shifted bacterial and fungal community structure as dominant bacterial and fungal groups differed in the direction and intensity of their responses. Ectomycorrhizal and saprotrophic fungal communities with greater connectivity (higher prevalence of strongly interconnected taxa based on pairwise co-occurrence relationships) were more resistant to compositional change. Warming effects on soil enzymes involved in the hydrolytic and oxidative liberation of carbon from plant cell walls and nutrients from organic matter were most strongly linked to fungal community responses, although community structure–function relationships differed between fungal guilds. Collectively, these findings indicate that warming and disturbance will influence the composition and function of microbial communities in the temperate-boreal ecotone, and fungal responses are particularly important to understand for predicting future ecosystem functioning.

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