Sources of Resistance to Fusarium solani and Associated Genomic Regions in Common Bean Diversity Panels

Common bean (Phaseolus vulgaris) cultivars with a high degree of resistance to Xanthomonas axonopodis pv. phaseoli (Xap) are not available in Brazil. Despite many studies, a low degree of resistance to Xap continues to exist due to its complex genetic inheritance, which is not well known. The objectives of this research were to complement a common bean genetic map based on the cross between a susceptible genotype 'HAB-52' and a resistant genotype 'BAC-6', and to map and analyze genomic regions (quantitative trait loci – QTLs) related to Xap resistance. Eleven linkage groups were determined using 143 RAPD markers, covering 1,234.5 cM of the genome. This map was used to detect QTLs associated with Xap resistance on leaves and pods. The averages of disease severity on leaves (represented by the transformed disease index – TDI) and pods (represented by the diameter of lesion on pods – DLP) were added to the data of the linkage map. Five TDI QTLs and only one LDP QTL were detected. The TDI QTLs were placed in the A, B, G and J linkage groups, with phenotypic variations ranging from 12.7 to 71.6%. The DLP QTL explained 12.9% of the phenotypic variation and was mapped in a distinct linkage group. These results indicate that there are different genes involved in the control of resistance on leaves and pods.

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Identification of new resistant sources against downy mildew disease from a selected set of cucumber germplasm and its wild relatives

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.4.8 ◽

2020 ◽

Vol 80 (04) ◽

Author(s):

Jameel Akhtar ◽

Dikshant Gautam ◽

Ranjan Nath ◽

A. B. Gaikwad ◽

K. V. Bhat ◽

...

Keyword(s):

Downy Mildew ◽

Plant Disease ◽

Mapping Population ◽

Disease Index ◽

Sources Of Resistance ◽

Resistant Accession ◽

Genomic Regions ◽

Moderately Resistant ◽

Agroclimatic Zones ◽

Downy Mildew Disease

This study was undertaken to identify new sources of resistance against downy mildew of cucumber caused by Pseudoperonospora cubensis [(Berk. and Cart.) Rostow]. Pot experiments were conducted with 16 accessions each of Cucumis sativus and C. hardwickii during the years 2017 and 2018 using 12 isolates of P. cubensis representing different agroclimatic zones of India. The accession, IC331627 from Dehradun, Uttarakhand showed plant disease index (PDI) ranging from 5.55-20.35 per cent with an average PDI of 11.56 and 11.87 per cent during the years, 2017 and 2018, respectively. Hence, IC331627 was identified to be resistant against the five isolates of P. cubesis (Pc12, Pc17, Pc19, Pc21 and Pc24) and moderately resistant against one isolate (Pc22). This resistant accession C. hardwickii (IC331627) could be utilized to develop mapping population to map genomic regions conferring the resistance to downy mildew in cucumber.

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Characterization of Colletotrichum lindemuthianum Races in Zambia and Evaluation of the CIAT Phaseolus Core Collection for Resistance to Anthracnose

Plant Disease ◽

10.1094/pdis-02-21-0363-re ◽

2021 ◽

Author(s):

Kelvin Kamfwa ◽

Paul Gepts ◽

Swivia Hamabwe ◽

Zombe Kapata Nalupya ◽

Chikoti Mukuma ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Core Collection ◽

Durable Resistance ◽

Colletotrichum Lindemuthianum ◽

Sources Of Resistance ◽

Runner Bean ◽

Causal Pathogen ◽

Scarlet Runner Bean

Colletotrichum lindemuthianum, the causal pathogen of common bean (Phaseolus vulgaris) anthracnose, is highly variable. Therefore, understanding its race structure and identification of new sources of resistance is necessary for the development of varieties with durable resistance. The objectives of this study were (i) to characterize isolates of C. lindemuthianum collected from three major bean-growing regions in Zambia, and (ii) evaluate the CIAT Phaseolus core collection for resistance to C. lindemuthianum races 37, 73, and 566, and a blend of 20 races. Isolates collected from three major bean-growing districts in Zambia, namely Mporokoso, Mpika, and Mbala, were characterized as race 37, 73, and 566, respectively. A subset of the CIAT core collection comprised of 885 accessions of common bean, 13 accessions of scarlet runner bean (P. coccineus), and 11 accessions of year bean (P. dumosus) were evaluated for resistance to races 37, 73 and 566, and a blend of 20 races in a greenhouse at University of Zambia, Lusaka, Zambia. A total of 72%, 66%, 48% and 9% of P. vulgaris accessions evaluated were highly resistant to races 37, 73, 566 and a blend of 20 races, respectively. Also, accessions of P. coccineus and P. dumosus, highly resistant to races 37, 73 and 566, were identified. Only eight of the 331 P. vulgaris accessions were highly resistant to all three individual races (37, 73, and 566) and to a blend of 20 races. These eight accessions constitute a valuable breeding resource for developing varieties with durable resistance to C. lindemuthianum.

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Author response for "Identification of sources of resistance to common bacterial blight in common bean in Ethiopia"

10.1111/jph.12951/v3/response1 ◽

2020 ◽

Author(s):

Kidane Tumsa ◽

Hussein Shimelis ◽

Mark Laing ◽

Clare Mukankusi ◽

Isack Mathew

Keyword(s):

Common Bean ◽

Bacterial Blight ◽

Author Response ◽

Common Bacterial Blight ◽

Sources Of Resistance

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Natural Variation in Portuguese Common Bean Germplasm Reveals New Sources of Resistance Against Fusarium oxysporum f. sp. phaseoli and Resistance-Associated Candidate Genes

Phytopathology ◽

10.1094/phyto-06-19-0207-r ◽

2020 ◽

Vol 110 (3) ◽

pp. 633-647 ◽

Cited By ~ 2

Author(s):

Susana T. Leitão ◽

Marcos Malosetti ◽

Qijan Song ◽

Fred van Eeuwijk ◽

Diego Rubiales ◽

...

Keyword(s):

Common Bean ◽

Fusarium Oxysporum ◽

Candidate Genes ◽

Fusarium Wilt ◽

Functional Markers ◽

Gene Pools ◽

Primary Metabolism ◽

Sources Of Resistance ◽

Wilt Resistance ◽

Fusarium Wilt Resistance

Common bean (Phaseolus vulgaris) is one of the most consumed legume crops in the world, and Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli, is one of the major diseases affecting its production. Portugal holds a very promising common bean germplasm with an admixed genetic background that may reveal novel genetic resistance combinations between the original Andean and Mesoamerican gene pools. To identify new sources of Fusarium wilt resistance and detect resistance-associated single-nucleotide polymorphisms (SNPs), we explored, for the first time, a diverse collection of the underused Portuguese common bean germplasm by using genome-wide association analyses. The collection was evaluated for Fusarium wilt resistance under growth chamber conditions, with the highly virulent F. oxysporum f. sp. phaseoli strain FOP-SP1 race 6. Fourteen of the 162 Portuguese accessions evaluated were highly resistant and 71 intermediate. The same collection was genotyped with DNA sequencing arrays, and SNP–resistance associations were tested via a mixed linear model accounting for the genetic relatedness between accessions. The results from the association mapping revealed nine SNPs associated with resistance on chromosomes Pv04, Pv05, Pv07, and Pv08, indicating that Fusarium wilt resistance is under oligogenic control. Putative candidate genes related to phytoalexin biosynthesis, hypersensitive response, and plant primary metabolism were identified. The results reported here highlight the importance of exploring underused germplasm for new sources of resistance and provide new genomic targets for the development of functional markers to support selection in future disease resistance breeding programs.

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Root-rot resistance in common bean

Canadian Journal of Plant Science ◽

10.4141/cjps93-054 ◽

1993 ◽

Vol 73 (1) ◽

pp. 365-367 ◽

Cited By ~ 11

Author(s):

J. C. Tu ◽

S. J. Park

Keyword(s):

Phaseolus Vulgaris ◽

Rhizoctonia Solani ◽

Common Bean ◽

Fusarium Oxysporum ◽

Key Words ◽

Fusarium Solani ◽

Root Rot ◽

Pythium Ultimum ◽

Growth Room

A bean (Phaseolus vulgaris) line, A - 300, resistant to Rhizoctonia solani and Fusarium oxysporum was introduced into Ontario from Colombia. The results of tests conducted in a root-rot nursery, in a greenhouse and in a growth room showed that this bean line is resistant to Fusarium solani f. sp. phaseoli and Pythium ultimum. Key words: Bean, Phaseolus vulgaris, root rot resistance

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A study on common bean cultivars to identify sources of resistance against the black bean aphid,Aphis fabaeScopoli (Hemiptera: Aphididae)

Archives of Phytopathology and Plant Protection ◽

10.1080/03235408.2013.772351 ◽

2013 ◽

Vol 46 (13) ◽

pp. 1598-1608 ◽

Cited By ~ 2

Author(s):

Mojtaba Esmaeili-Vardanjani ◽

Alireza Askarianzadeh ◽

Zarir Saeidi ◽

Gholam Hossein Hasanshahi ◽

Jaber Karimi ◽

...

Keyword(s):

Common Bean ◽

Sources Of Resistance ◽

Black Bean ◽

Black Bean Aphid

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Genome conservation among three legume genera detected with DNA markers

Genome ◽

10.1139/g95-122 ◽

1995 ◽

Vol 38 (5) ◽

pp. 928-937 ◽

Cited By ~ 68

Author(s):

S. R. Boutin ◽

N. D. Young ◽

T. C. Olson ◽

Z.-H. Yu ◽

C. E. Vallejos ◽

...

Keyword(s):

Glycine Max ◽

Common Bean ◽

Dna Markers ◽

Marker Density ◽

Linkage Maps ◽

Linkage Groups ◽

Comparative Maps ◽

Genomic Regions ◽

High Degree ◽

Linkage Conservation

A set of 219 DNA clones derived from mungbean (Vigna radiata), cowpea (V. unguiculata), common bean (Phaseolus vulgaris), and soybean (Glycine max) were used to generate comparative linkage maps among mungbean, common bean, and soybean. The maps allowed an assessment of linkage conservation and collinearity among the three genomes. Mungbean and common bean, both of the subtribe Phaseolinae, exhibited a high degree of linkage conservation and preservation of marker order. Most linkage groups of mungbean consisted of only one or two linkage blocks from common bean (and vice versa). The situation was significantly different with soybean, a member of the subtribe Glycininae. Mungbean and common bean linkage groups were generally mosaics of short soybean linkage blocks, each only a few centimorgans in length. These results suggest that it would be fruitful to join maps of mungbean and common bean, while knowledge of conserved genomic blocks would be useful in increasing marker density in specific genomic regions for all three genera. These comparative maps may also contribute to enhanced understanding of legume evolution.Key words: RFLP, gene mapping, Phaseolus, Glycine, Vigna.

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Genetic Mapping Reveals Complex Architecture and Candidate Genes Involved in Common Bean Response to Meloidogyne Incognita Infection

10.21203/rs.3.rs-305996/v1 ◽

2021 ◽

Author(s):

Willian Giordani ◽

Henrique Castro Gama ◽

Alisson Fernando Chiorato ◽

João Paulo Rodrigues Marques ◽

Luis Eduardo Aranha Camargo ◽

...

Keyword(s):

Common Bean ◽

Candidate Genes ◽

Resistance Genes ◽

Meloidogyne Incognita ◽

Genetic Architecture ◽

Association Studies ◽

Genome Wide Association Studies ◽

Crop Species ◽

Egg Masses ◽

Genomic Regions

Abstract Root-knot nematodes (RKN), particularly Meloidogyne incognita, are among the most damaging and prevalent agricultural pathogens due to their ability to infect roots of almost all crop species, including common bean. The best strategy for their control is through the use of resistant cultivars. However, laborious phenotyping procedures make it difficult to assess nematode resistance in breeding programs. For common bean, this task is especially challenging since little has been done to discover resistance genes or find markers to assist selection. In this study, we performed genome-wide association studies and QTL mapping to explore the genetic architecture and genomic regions underlying the resistance to M. incognita and to identify candidate resistance genes. Phenotypic data were collected by a high-throughput assay, and the number of egg masses and root-galling index were evaluated 30 days after inoculation. Complex genetic architecture and independent genomic regions were associated with each trait according to the Fixed and random model Circulating Probability Unification. SNPs located on chromosomes Pv06, Pv07, Pv08 and Pv11 were associated with the number of egg masses, and on Pv01, Pv02, Pv05 and Pv10 with root-galling. A total of 215 candidate genes were identified, including 14 resistance gene analogs and five differentially expressed in a previous RNA-seq analysis. The histochemical analysis indicated that the reactive oxygen species might play a role in the resistance response. Our findings open new perspectives to improve selection efficiency for RKN resistance in common bean, and the candidate genes are valuable targets for functional investigation and gene editing approaches.

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Genetic Associations in Four Decades of Multi-Environment Trials Reveal Agronomic Trait Evolution in Common Bean

10.1101/734087 ◽

2019 ◽

Author(s):

Alice H. MacQueen ◽

Jeffrey W. White ◽

Rian Lee ◽

Juan M. Osorno ◽

Jeremy Schmutz ◽

...

Keyword(s):

Common Bean ◽

Crop Improvement ◽

Genetic Research ◽

The United States ◽

Genetic Effects ◽

Data Sets ◽

Genetic Associations ◽

Phenotypic Data ◽

Multiple Phenotypes ◽

Genomic Regions

AbstractMulti-environment trials (METs) are widely used to assess the performance of promising crop germplasm. Though seldom designed to elucidate genetic mechanisms, MET datasets are often much larger than could be duplicated for genetic research and, given proper interpretation, may offer valuable insights into the genetics of adaptation across time and space. The Cooperative Dry Bean Nursery (CDBN) is a MET for common bean (Phaseolus vulgaris) grown for over 70 years in the United States and Canada, consisting of 20 to 50 entries each year at 10 to 20 locations. The CBDN provides a rich source of phenotypic data across entries, years, and locations that is amenable to genetic analysis. To study stable genetic effects segregating in this MET, we conducted genome-wide association (GWAS) using best linear unbiased predictions (BLUPs) derived across years and locations for 21 CDBN phenotypes and genotypic data (1.2M SNPs) for 327 CDBN genotypes. The value of this approach was confirmed by the discovery of three candidate genes and genomic regions previously identified in balanced GWAS. Multivariate adaptive shrinkage (mash) analysis, which increased our power to detect significant correlated effects, found significant effects for all phenotypes. The first use of mash on an agricultural dataset discovered two genomic regions with pleiotropic effects on multiple phenotypes, likely selected on in pursuit of a crop ideotype. Overall, our results demonstrate that by applying multiple statistical genomic approaches on data mined from MET phenotypic data sets, significant genetic effects that define genomic regions associated with crop improvement can be discovered.

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