scholarly journals Natural Variation in Portuguese Common Bean Germplasm Reveals New Sources of Resistance Against Fusarium oxysporum f. sp. phaseoli and Resistance-Associated Candidate Genes

2020 ◽  
Vol 110 (3) ◽  
pp. 633-647 ◽  
Author(s):  
Susana T. Leitão ◽  
Marcos Malosetti ◽  
Qijan Song ◽  
Fred van Eeuwijk ◽  
Diego Rubiales ◽  
...  
Keyword(s):  
Common Bean ◽  
Fusarium Oxysporum ◽  
Candidate Genes ◽  
Fusarium Wilt ◽  
Functional Markers ◽  
Gene Pools ◽  
Primary Metabolism ◽  
Sources Of Resistance ◽  
Wilt Resistance ◽  
Fusarium Wilt Resistance

Common bean (Phaseolus vulgaris) is one of the most consumed legume crops in the world, and Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli, is one of the major diseases affecting its production. Portugal holds a very promising common bean germplasm with an admixed genetic background that may reveal novel genetic resistance combinations between the original Andean and Mesoamerican gene pools. To identify new sources of Fusarium wilt resistance and detect resistance-associated single-nucleotide polymorphisms (SNPs), we explored, for the first time, a diverse collection of the underused Portuguese common bean germplasm by using genome-wide association analyses. The collection was evaluated for Fusarium wilt resistance under growth chamber conditions, with the highly virulent F. oxysporum f. sp. phaseoli strain FOP-SP1 race 6. Fourteen of the 162 Portuguese accessions evaluated were highly resistant and 71 intermediate. The same collection was genotyped with DNA sequencing arrays, and SNP–resistance associations were tested via a mixed linear model accounting for the genetic relatedness between accessions. The results from the association mapping revealed nine SNPs associated with resistance on chromosomes Pv04, Pv05, Pv07, and Pv08, indicating that Fusarium wilt resistance is under oligogenic control. Putative candidate genes related to phytoalexin biosynthesis, hypersensitive response, and plant primary metabolism were identified. The results reported here highlight the importance of exploring underused germplasm for new sources of resistance and provide new genomic targets for the development of functional markers to support selection in future disease resistance breeding programs.

Detection and Mapping of a Major Locus for Fusarium Wilt Resistance in Common Bean

Crop Science ◽  
2001 ◽  
Vol 41 (5) ◽  
pp. 1494-1498 ◽  
Author(s):  
A. L. Fall ◽  
P. F. Byrne ◽  
G. Jung ◽  
D. P. Coyne ◽  
M. A. Brick ◽  
...  
Keyword(s):  
Common Bean ◽  
Fusarium Wilt ◽  
Wilt Resistance ◽  
Major Locus ◽  
Fusarium Wilt Resistance

scholarly journals Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Yujie Chang ◽  
Feifei Sun ◽  
Suli Sun ◽  
Lanfen Wang ◽  
Jing Wu ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Fusarium Wilt ◽  
Transcriptome Analysis ◽  
Stress Responses ◽  
Mung Bean ◽  
Differential Expression Analysis ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Wilt Resistance ◽  
Fusarium Wilt Resistance

Fusarium wilt is a destructive soil-borne disease that threatens the production of mung bean. Mung bean lines Zheng8-4 and Zheng8-20 show high resistance and high susceptibility to Fusarium wilt, respectively. Transcriptome analysis was carried out to identify candidate genes involved in Fusarium wilt resistance using Zheng8-4 and Zheng8-20 at 0, 0.5, 1, 2, and 4 days post inoculation (dpi). Differential expression analysis showed that 3,254 genes responded to pathogen infection and were differentially expressed in the resistant and susceptible lines. Weighted gene co-expression network analysis (WGCNA) was also performed to identify five modules highly correlated with Fusarium wilt resistance, in which 453 differentially expressed genes (DEGs) were considered likely to be involved in Fusarium wilt resistance. Among these DEGs, we found 24 genes encoding resistance (R) proteins, 22 encoding protein kinases, 20 belonging to transcription factor families, 34 encoding proteins with oxidoreductase activity, 17 involved in stimulation/stress responses, and 54 annotated to pathogen resistance-related pathways. Finally, 27 annotated genes were further selected as candidate genes of Fusarium wilt resistance in mung bean. This study identifies novel potential resistance-related genes against Fusarium wilt and provides a theoretical basis for further investigation of Fusarium wilt resistance in mung bean breeding.

Discovery of a new source of resistance to Fusarium oxysporum, cause of Fusarium wilt in Allium fistulosum, located on chromosome 2 of Allium cepa Aggregatum group

Genome ◽  
2012 ◽  
Vol 55 (11) ◽  
pp. 797-807 ◽  
Author(s):  
Hoa Q. Vu ◽  
Magdi A. El-Sayed ◽  
Shin-Ichi Ito ◽  
Naoki Yamauchi ◽  
Masayoshi Shigyo
Keyword(s):  
Fusarium Oxysporum ◽  
Allium Cepa ◽  
Fusarium Wilt ◽  
Root Exudates ◽  
Allium Fistulosum ◽  
Addition Lines ◽  
Wilt Resistance ◽  
Monosomic Addition ◽  
Monosomic Addition Lines ◽  
Fusarium Wilt Resistance

This study was carried out to evaluate the antifungal effect of Allium cepa Aggregatum group (shallot) metabolites on Fusarium oxysporum and to determine the shallot chromosome(s) related to Fusarium wilt resistance using a complete set of eight Allium fistulosum – shallot monosomic addition lines. The antifungal effects of hexane, butanol, and water extraction fractions from bulbs of shallot on 35 isolates of F. oxysporum were examined using the disc diffusion method. Only hexane and butanol fractions showed high antifungal activity. Shallot showed no symptom of disease after inoculation with F. oxysporum f. sp. cepae. The phenolic content of the roots and the saponin content of root exudates of inoculated shallot increased to much higher levels than those of the control at 3 days after inoculation. Application of freeze-dried shallot root exudates to seeds of A. fistulosum soaked in a spore suspension of F. oxysporum resulted in protection of seedlings against infection. Among eight monosomic addition lines and A. fistulosum, FF+2A showed the highest resistance to Fusarium wilt. This monosomic addition line also showed a specific saponin band derived from shallot on the thin layer chromatography profile of saponins in the eight monosomic addition lines. The chromosome 2A of shallot might possess some of the genes related to Fusarium wilt resistance.

scholarly journals A Methyl Esterase 1 (PvMES1) Promotes the Salicylic Acid Pathway and Enhances Fusarium Wilt Resistance in Common Beans

2021 ◽  
Author(s):  
Renfeng Xue ◽  
Ming Feng ◽  
Jian Chen ◽  
Weide Ge ◽  
Matthew W. Blair
Keyword(s):  
Salicylic Acid ◽  
Common Bean ◽  
Signaling Pathway ◽  
Fusarium Wilt ◽  
Defense Response ◽  
Sugar Metabolism ◽  
Common Beans ◽  
Wilt Resistance ◽  
Practical Applications ◽  
Fusarium Wilt Resistance

Abstract Common bean (Phaseolus vulgaris L.) is an important food legume. Fusarium wilt caused by Fusarium oxysporum f. sp. phaseoli is one of the most serious soil-born diseases of common bean found throughout the world and affects the yield and quality of the crop. Few sources of Fusarium wilt resistance exist in legumes and most are of quantitative inheritance. In this study, we have identified a methyl salicylate esterase (MES), PvMES1, that contributes to plant defense response by regulating the salicylic acid (SA) mediated signaling pathway in response to Fusarium wilt in common beans. The result showed the role of PvMES1 in regulating SA levels in common bean and thus the SA signaling pathway and defense response mechanism in the plant. Overexpression of the PvMES1 gene enhanced Fusarium wilt resistance; while silencing of the gene caused susceptibility to the diseases. RNA-seq analysis with these transiently modified plants showed that genes related to SA level changes included the following gene ontologies: a) interaction between host and pathogen; b) phenylpropanoid synthesis; and c) sugar metabolism as well as others. These key signal elements activated the defense response pathway in common bean to Fusarium wilt. Collectively, our findings indicate that PvMES1 plays a pivotal role in regulating SA biosynthesis and signaling, and increasing Fusarium wilt resistance in common bean, thus providing novel insight into the practical applications of both SA and MES genes and pathways they contribiute to for developing elite crop varieties with enhanced broad-spectrum resistance to this critical disease.

Cloning and Expression Analysis of Fusarium Wilt Resistance-Related Gene PvCaM1 in Common Bean (Phaseolus vulgaris L.)

2013 ◽  
Vol 38 (4) ◽  
pp. 606-613
Author(s):  
Ren-Feng XUE ◽  
Zhen-Dong ZHU ◽  
Xiao-Ming WANG ◽  
Lan-Fen WANG ◽  
Xiao-Fei WU ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Fusarium Wilt ◽  
Expression Analysis ◽  
Cloning And Expression ◽  
Related Gene ◽  
Wilt Resistance ◽  
Fusarium Wilt Resistance

scholarly journals QTL mapping for Fusarium wilt resistance based on the whole-genome resequencing and their association with functional genes in Raphanus sativus

2021 ◽  
Author(s):  
Yinbo Ma ◽  
Sushil Satish Chhapekar ◽  
Lu Lu ◽  
Xiaona Yu ◽  
Seungho Kim ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Phenotypic Variation ◽  
Pcr Analysis ◽  
Potential Candidate ◽  
Sequencing Data ◽  
Wilt Resistance ◽  
Protein Levels ◽  
Trait Locus ◽  
Fusarium Wilt Resistance

Abstract Fusarium wilt is an important disease of radish, leading to severe decrease in yield and quality. In this study, we used 180 F2 populations derived from a cross between radish inbred lines ‘YR4’ and ‘YR18’ to construct linkage group for the detecting quantitative trait locus (QTLs) related to Fusarium oxysporum resistance. Four QTLs related to Fusarium oxysporum resistance were detected on two linkage groups. Of the two major loci for FW resistance, FoRsR7.1FOR59 was delimited to the 2.18-Mb physical interval on chromosome R07, with a high LOD value (5.17–12.84). Additionally, it explained 9.34%-27.97% of the phenotypic variation in three inoculation tests. The other major QTL, FoRsR9.3FOR59, was also detected in all inoculation tests, but it had a relatively low value (3.38–4.52) and explained 6.24%-8.82% of the phenotypic variation. On the basis of the re-sequencing data for the parental lines, we identified 5 R-related genes and 13 unknown genes with sequence variations at the gene and protein levels. A semi-quantitative RT-PCR analysis revealed Rs382940 (TIR-NBS type) and Rs382200 (RLK type) were expressed only in ‘YR4’ from 0 to 6 days after the inoculation. Moreover, Rs382950 (TIR-NBS-LRR type) was more highly expressed in ‘YR4’ from 3 and 6 days after the inoculation. Therefore, these three genes are likely important for the FW resistance of radish. We identified several markers based on the potential candidate genes. These gene set might be useful for breeding system to introduce the Fusarium wilt resistance loci from ‘YR4’ to improve increasing tolerance to Fusarium oxysporum.

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