Mapping and Validation of Major Quantitative Trait Loci for Resistance to Northern Corn Leaf Blight Along With the Determination of the Relationship Between Resistances to Multiple Foliar Pathogens of Maize (Zea mays L.)

Among various foliar diseases affecting maize yields worldwide, northern corn leaf blight (NCLB) is economically important. The genetics of resistance was worked out to be quantitative in nature thereby suggesting the need for the detection of quantitative trait loci (QTL) to initiate effective marker-aided breeding strategies. From the cross CML153 (susceptible) × SKV50 (resistant), 344 F2:3 progenies were derived and screened for their reaction to NCLB during the rainy season of 2013 and 2014. The identification of QTL affecting resistance to NCLB was carried out using the genetic linkage map constructed with 194 polymorphic SNPs and the disease data recorded on F2:3 progeny families. Three QTL for NCLB resistance were detected on chromosomes 2, 5, and 8 with the QTL qNCLB-8-2 explaining the highest phenotypic variation of 16.34% followed by qNCLB-5 with 10.24%. QTL for resistance to sorghum downy mildew (SDM) and southern corn rust (SCR) were also identified from one season phenotypic data, and the co-location of QTL for resistance to three foliar diseases was investigated. QTL present in chromosome bins 8.03, 5.03, 5.04, and 3.04 for resistance to NCLB, SDM, and SCR were co-localized, indicating their usefulness for the pyramiding of quantitative resistance to multiple foliar pathogens. Marker-assisted selection was practiced in the crosses CM212 × SKV50, HKI162 × SKV50, and CML153 × SKV50 employing markers linked to major QTL on chromosomes 8, 2, and 10 for NCLB, SDM, and SCR resistance, respectively. The populations were advanced to F6 stage to derive multiple disease-resistant inbred lines. Out of the 125 lines developed, 77 lines were tested for their combining ability and 39 inbred lines exhibited high general combining ability with an acceptable level of resistance to major diseases.

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Quantitative Trait Loci in Sweet Corn Associated with Partial Resistance to Stewart's Wilt, Northern Corn Leaf Blight, and Common Rust

Phytopathology ◽

10.1094/phyto.2001.91.3.293 ◽

2001 ◽

Vol 91 (3) ◽

pp. 293-300 ◽

Cited By ~ 34

Author(s):

A. F. Brown ◽

J. A. Juvik ◽

J. K. Pataky

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Partial Resistance ◽

Sweet Corn ◽

Phenotypic Variability ◽

Leaf Blight ◽

Northern Corn Leaf Blight ◽

Trait Loci ◽

Stewart's Wilt ◽

Corn Leaf Blight

Partial resistance to Stewart's wilt (Erwina stewartii, syn. Pantoea stewartii), northern corn leaf blight (NCLB) (Exserohilum turcicum), and common rust (Puccinia sorghi) was observed in an F2:3 population developed from a cross between the inbred sweet corn lines IL731a and W6786. The objective of this study was to identify quantitative trait loci (QTL) associated with partial resistance using restriction fragment length polymorphic markers. Phenotypic data were collected for 2 years for Stewart's wilt, NCLB, and common rust but, due to significant family-environment interaction, analysis was conducted individually on data from each year. In 2 years of evaluation for the three diseases, a total of 33 regions in the maize genome were associated with partial resistance describing from 5.9 to 18% of the total phenotypic variability. Of six regions common in both years, three were associated with partial resistance to Stewart's wilt (chromosomes 4:07, 5:03, and 6:04), one was associated with NCLB (chromosome 9:05), and two were associated with common rust (chromosomes 2:04 and 3:04). The rust QTL on 3S mapped to within 20 cM of the rp3 locus and explained 17.7% of the phenotypic variability. Some of the QTL associated with partial resistance to the three diseases have been reported previously, and some are described here for the first time. Results suggest it may be possible to consolidate QTL from various elite backgrounds in a manner analogous to the pyramiding of major resistance genes. We also report here on two QTL associated with anthocyanin production on chromosomes 10:6 and 5:03 in the general location of the a2 gene.

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Genetic mapping of northern corn leaf blight-resistant quantitative trait loci in maize

Medicine ◽

10.1097/md.0000000000021326 ◽

2020 ◽

Vol 99 (31) ◽

pp. e21326

Author(s):

Haifeng Xia ◽

Wei Gao ◽

Jing Qu ◽

Liqiang Dai ◽

Yan Gao ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Genetic Mapping ◽

Quantitative Trait ◽

Leaf Blight ◽

Northern Corn Leaf Blight ◽

Trait Loci ◽

Corn Leaf Blight

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Genetic Mapping And Genomic Prediction For Northern Corn Leaf Blight (Exserohilum Turcicum (Pass.) Leonard And Suggs) Resistance

10.21203/rs.3.rs-618501/v1 ◽

2021 ◽

Author(s):

D.C. Balasundara ◽

H. C. Lohithaswa ◽

M. Rahul ◽

R. L. Ravikumar ◽

Anand Pandravada ◽

...

Keyword(s):

Genomic Prediction ◽

Combining Ability ◽

Inbred Lines ◽

Leaf Blight ◽

Environment Interaction ◽

Exserohilum Turcicum ◽

Northern Corn Leaf Blight ◽

Polymorphic Snps ◽

Corn Leaf Blight ◽

Two Populations

Abstract Background: Northern corn leaf blight (NCLB) of maize caused by Exserohilum turcicum is a serious foliar disease. Resistance to NCLB is complexly inherited and the highly significant genotype x environment interaction effect makes selection of resistant genotypes difficult through conventional breeding methods. Hence an attempt was made to identify the genomic regions associated with NCLB resistance and perform genomic selection (GS) in two F2:3 populations derived from the crosses CM212 × MAI172 (Population-1) and CM202 × SKV50 (Population-2). Results: Two populations, each comprising of 366 progenies, were phenotyped at three different locations in the disease screening nurseries. Linkage analysis using 297 polymorphic SNPs in Population-1 and 290 polymorphic SNPs in Population-2 revealed 10 linkage groups spanning 3623.88cM and 4261.92cM with an average distance of 12.40 cM and 14.9 cM, respectively. Location-wise and pooled data across locations indicated that QTL expression was population and environment specific. The genomic prediction accuracies of 0.83 and 0.79 were achieved for NCLB Population 1 and Population 2, respectively. The resistant progenies from both populations were advanced to derive inbred lines and crossed with four different testers in line x tester mating design to test for their combining ability. High overall general combining ability was exhibited by 21 inbred lines. Among crosses 48 % were assigned high overall specific combining ability status. Out of 136 single crosses, seven recorded significant positive standard heterosis over the best check for grain yield. The clustering pattern of inbred lines developed from the two populations revealed high molecular diversity. Conclusions: In this study, comparatively better genomic prediction accuracies were achieved for NCLB and the worth of F3 progenies with high genomic predictions was proved by advancing them to derive inbred lines and establishing their higher combining ability for yield and yield related traits.

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Heterosis and combining ability of elite maize inbred lines under northern corn leaf blight disease prone environments of the mid-altitude tropics

Euphytica ◽

10.1007/s10681-015-1619-5 ◽

2015 ◽

Vol 208 (2) ◽

pp. 391-400 ◽

Cited By ~ 5

Author(s):

Wende Abera ◽

Shimelis Hussein ◽

John Derera ◽

Mosisa Worku ◽

Mark Laing

Keyword(s):

Combining Ability ◽

Inbred Lines ◽

Leaf Blight ◽

Northern Corn Leaf Blight ◽

Maize Inbred Lines ◽

Blight Disease ◽

Corn Leaf Blight

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Mapping Resistance Quantitative Trait Loci for Three Foliar Diseases in a Maize Recombinant Inbred Line Population—Evidence for Multiple Disease Resistance?

Phytopathology ◽

10.1094/phyto-100-1-0072 ◽

2010 ◽

Vol 100 (1) ◽

pp. 72-79 ◽

Cited By ~ 57

Author(s):

John C. Zwonitzer ◽

Nathan D. Coles ◽

Matthew D. Krakowsky ◽

Consuelo Arellano ◽

James B. Holland ◽

...

Keyword(s):

Disease Resistance ◽

Quantitative Trait Loci ◽

Inbred Line ◽

Recombinant Inbred Line ◽

Quantitative Trait ◽

Recombinant Inbred ◽

Leaf Blight ◽

Foliar Diseases ◽

Multiple Disease Resistance ◽

Trait Loci

Southern leaf blight (SLB), gray leaf spot (GLS), and northern leaf blight (NLB) are all important foliar diseases impacting maize production. The objectives of this study were to identify quantitative trait loci (QTL) for resistance to these diseases in a maize recombinant inbred line (RIL) population derived from a cross between maize lines Ki14 and B73, and to evaluate the evidence for the presence genes or loci conferring multiple disease resistance (MDR). Each disease was scored in multiple separate trials. Highly significant correlations between the resistances and the three diseases were found. The highest correlation was identified between SLB and GLS resistance (r = 0.62). Correlations between resistance to each of the diseases and time to flowering were also highly significant. Nine, eight, and six QTL were identified for SLB, GLS, and NLB resistance, respectively. QTL for all three diseases colocalized in bin 1.06, while QTL colocalizing for two of the three diseases were identified in bins 1.08 to 1.09, 2.02/2.03, 3.04/3.05, 8.05, and 10.05. QTL for time to flowering were also identified at four of these six loci (bins 1.06, 3.04/3.05, 8.05, and 10.05). No disease resistance QTL was identified at the largest-effect QTL for flowering time in bin 10.03.

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Analysis of Quantitative Trait Loci for Resistance to Southern Leaf Blight in Juvenile Maize

Phytopathology ◽

10.1094/phyto-96-0221 ◽

2006 ◽

Vol 96 (3) ◽

pp. 221-225 ◽

Cited By ~ 30

Author(s):

P. J. Balint-Kurti ◽

M. L. Carson

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Quantitative Resistance ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Leaf Blight ◽

Growth Stages ◽

Trait Loci ◽

Southern Leaf Blight ◽

Juvenile Plants

A set of 192 maize recombinant inbred lines (RILs), derived from a cross between the inbred lines Mo17 and B73, were evaluated as 3-week-old seedlings in the greenhouse for resistance to southern leaf blight, caused by Cochliobolus heterostrophus race O. Six significant (LOD >3.1) quantitative trait loci (QTL) were identified for disease resistance, located on chromosomes 1, 2, 3, 6, 7, and 8. Results were compared with a previous study that had used the same RIL population and pathogen isolate, but had examined resistance in mature rather than juvenile plants. There was a very weak but significant correlation between the overall resistance phenotypes of the RILs scored as mature and juvenile plants. Two QTL were found in similar positions on chromosomes 1 and 3 at both growth stages. Other QTL were specific to one growth stage or the other. Twenty-three of these RILs, together with the parental lines, were inoculated in the greenhouse with four C. heterostrophus isolates. Results indicated that the quantitative resistance observed was largely isolate non-specific.

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