scholarly journals Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber

2021 ◽  
Vol 12 ◽  
Author(s):  
Siyuan Zhang ◽  
Yina Shao ◽  
Chenghua Li
Keyword(s):  
Sea Cucumber ◽  
Apostichopus Japonicus ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Vibrio Splendidus ◽  
Host Interactions ◽  
Competing Endogenous Rnas ◽  
Mirna Sponges ◽  
And Function

Long non-coding RNAs (lncRNAs) have been reported to play critical roles during pathogen infection and innate immune response in mammals. Such observation inspired us to explore the expression profiles and functions of lncRNAs in invertebrates upon bacterial infection. Here, the lncRNAs of sea cucumber (Apostichopus japonicus) involved in Vibrio splendidus infection were characterized. RNA-seq obtained 2897 differentially expressed lncRNAs from Vibrio splendidus infected coelomocytes of sea cucumbers. The potential functions of the significant differentially expressed lncRNAs were related to immunity and metabolic process based on the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Moreover, we identify a lncRNA (XLOC_028509), which is downregulated with Vibrio splendidus challenged, further study indicated that XLOC_028509 adsorb miR-2008 and miR-31 as competing endogenous RNAs (ceRNAs) through base complementarity, which in turn decreased the amount of miRNAs (microRNAs) bound to the 3’UTRs (untranslated regions) of mRNAs to reduce their inhibition of target gene translation. These data demonstrated that the lncRNAs of invertebrates might be important regulators in pathogen-host interactions by sponging miRNAs.

scholarly journals Comparative transcriptome analysis of papilla and skin in the sea cucumber,Apostichopus japonicus

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1779 ◽  
Author(s):  
Xiaoxu Zhou ◽  
Jun Cui ◽  
Shikai Liu ◽  
Derong Kong ◽  
He Sun ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transcriptome Analysis ◽  
Sea Cucumber ◽  
Molecular Mechanisms ◽  
Transforming Growth Factor ◽  
Apostichopus Japonicus ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Comparative Transcriptome ◽  
Comparative Transcriptome Analysis

Papilla and skin are two important organs of the sea cucumber. Both tissues have ectodermic origin, but they are morphologically and functionally very different. In the present study, we performed comparative transcriptome analysis of the papilla and skin from the sea cucumber (Apostichopus japonicus) in order to identify and characterize gene expression profiles by using RNA-Seq technology. We generated 30.6 and 36.4 million clean reads from the papilla and skin and de novo assembled in 156,501 transcripts. The Gene Ontology (GO) analysis indicated that cell part, metabolic process and catalytic activity were the most abundant GO category in cell component, biological process and molecular funcation, respectively. Comparative transcriptome analysis between the papilla and skin allowed the identification of 1,059 differentially expressed genes, of which 739 genes were expressed at higher levels in papilla, while 320 were expressed at higher levels in skin. In addition, 236 differentially expressed unigenes were not annotated with any database, 160 of which were apparently expressed at higher levels in papilla, 76 were expressed at higher levels in skin. We identified a total of 288 papilla-specific genes, 171 skin-specific genes and 600 co-expressed genes. Also, 40 genes in papilla-specific were not annotated with any database, 2 in skin-specific. Development-related genes were also enriched, such asfibroblast growth factor,transforming growth factor-β,collagen-α2andIntegrin-α2, which may be related to the formation of the papilla and skin in sea cucumber. Further pathway analysis identified ten KEGG pathways that were differently enriched between the papilla and skin. The findings on expression profiles between two key organs of the sea cucumber should be valuable to reveal molecular mechanisms involved in the development of organs that are related but with morphological differences in the sea cucumber.

Antioxidant enzymes in sea cucumber Apostichopus japonicus (Selenka) during aestivation

2010 ◽  
Vol 91 (1) ◽  
pp. 209-214 ◽  
Author(s):  
Wang Fangyu ◽  
Yang Hongsheng Yang ◽  
Wang Xiaoyu ◽  
Xing Kun ◽  
Gao Fei
Keyword(s):  
Oxygen Consumption ◽  
Glutathione Peroxidase ◽  
Sea Cucumber ◽  
Apostichopus Japonicus ◽  
Sampling Period ◽  
Coelomic Fluid ◽  
Sharp Change ◽  
Antioxidant Defence ◽  
Antioxidant Defences ◽  
And Function

To evaluate the effect of antioxidant defence in coelomic fluid of sea cucumber, Apostichopus japonicus in aestivation was studied in the field from July to November 2006 in Qingdao. During the sampling period, activities of superoxide dismutase and catalase increased significantly in August and November. Activities of glutathione reductase and glutathione decreased significantly in August and increased significantly in November and activities of Se-glutathione peroxidase increased significantly in August. There were no significant differences in total glutathione peroxidase. In relation to the water temperature in the field, it is known that the oxygen consumption rate dropped and antioxidant defence was enhanced in August. The structure and function of respiratory trees of A. japonicus were completely vivified as normal in November, and it is suggested that antioxidant defence was enhanced because of the sharp change of oxygen consumption. Data indicate that both enzymatic and metabolite antioxidant defences in sea cucumber are adaptable systems that are modulated during pre-aestivating stage and arousing stage.

Effects of dietary β-glucan on the growth, immune responses and resistance of sea cucumber, Apostichopus japonicus against Vibrio splendidus infection

Aquaculture ◽  
2011 ◽  
Vol 315 (3-4) ◽  
pp. 269-274 ◽  
Author(s):  
Yancui Zhao ◽  
Hongming Ma ◽  
Wenbing Zhang ◽  
Qinghui Ai ◽  
Kangsen Mai ◽  
...  
Keyword(s):  
Immune Responses ◽  
Sea Cucumber ◽  
Apostichopus Japonicus ◽  
Vibrio Splendidus

scholarly journals Molecular signatures associated with ZIKV exposure in human cortical neural progenitors

2016 ◽  
Author(s):  
Feiran Zhang ◽  
Christy Hammack ◽  
Sarah C. Ogden ◽  
Yichen Cheng ◽  
Emily M. Lee ◽  
...  
Keyword(s):  
Neural Progenitor Cells ◽  
Expression Profiles ◽  
Molecular Signatures ◽  
Zikv Infection ◽  
Host Interactions ◽  
Expression Of Genes ◽  
Dna Replication And Repair ◽  
Human Neural Progenitor Cells ◽  
Induced Apoptosis ◽  
And Function

AbstractZika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs brain development and function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed to Asian ZIKVC, African ZIKVM, and dengue virus (DENV). In contrast to the robust global transcriptome changes induced by DENV, ZIKV has a more selective and larger impact on expression of genes involved in DNA replication and repair. While overall expression profiles are similar, ZIKVC, but not ZIKVM, induces upregulation of viral response genes and TP53. P53 inhibitors can block the apoptosis induced by both ZIKVC and ZIKVM in hNPCs, with higher potency against ZIKVC-induced apoptosis. Our analyses reveal virus- and strain-specific molecular signatures associated with ZIKV infection. These datasets will help to investigate ZIKV-host interactions and identify neurovirulence determinants of ZIKV.

scholarly journals Development and characterization of 70 novel microsatellite markers for the sea cucumber (Apostichopus japonicus)

2012 ◽  
Vol 11 (1) ◽  
pp. 434-439 ◽  
Author(s):  
W. Peng ◽  
Z.M. Bao ◽  
H.X. Du ◽  
J.J. Yan ◽  
L.L. Zhang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Sea Cucumber ◽  
Apostichopus Japonicus
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