Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus on Botrytis spp.

ABSTRACT Bloodstream infections are a leading cause of morbidity and mortality. Early and targeted antimicrobial intervention is lifesaving, yet current diagnostic approaches fail to provide actionable information within a clinically viable time frame due to their reliance on blood culturing. Here, we present a novel pathogen identification (PID) platform that features the use of duplex DNA-invading γ-modified peptide nucleic acids (γPNAs) for the rapid identification of bacterial and fungal pathogens directly from blood, without culturing. The PID platform provides species-level information in under 2.5 hours while reaching single-CFU-per-milliliter sensitivity across the entire 21-pathogen panel. The clinical utility of the PID platform was demonstrated through assessment of 61 clinical specimens, which showed >95% sensitivity and >90% overall correlation to blood culture findings. This rapid γPNA-based platform promises to improve patient care by enabling the administration of a targeted first-line antimicrobial intervention. IMPORTANCE Bloodstream infections continue to be a major cause of death for hospitalized patients, despite significant improvements in both the availability of treatment options as well their application. Since early and targeted antimicrobial intervention is one of the prime determinants of patient outcome, the rapid identification of the pathogen can be lifesaving. Unfortunately, current diagnostic approaches for identifying these infections all rely on time-consuming blood culture, which precludes immediate intervention with a targeted antimicrobial. To address this, we have developed and characterized a new and comprehensive methodology, from patient specimen to result, for the rapid identification of both bacterial and fungal pathogens without the need for culturing. We anticipate broad interest in our work, given the novelty of our technical approach combined with an immense unmet need.

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Linitis Plastica of The Stomach: A Review

Journal of Surgical Sciences ◽

10.3329/jss.v22i2.44231 ◽

2020 ◽

Vol 22 (2) ◽

pp. 125-138

Author(s):

Md Mizanur Rahman

Keyword(s):

Gastric Cancer ◽

Treatment Outcome ◽

Surgical Resection ◽

Linitis Plastica ◽

Diffuse Gastric Cancer ◽

Biological Behaviour ◽

Desmoplastic Reaction ◽

The Poor ◽

Diagnostic Approaches ◽

Poor Outcomes

Linitis plastica (LP) is a particular subtype of diffuse gastric cancer and is thought to have a separate entity in respect with its biological behaviour, pathology, presentation and treatment outcome. The poor prognosis of LP gastric cancer is due primarily to its advanced stage at diagnosis. The characteristic histopathological feature of this entity is cellular spread to the submucosa and stroma with minimal mucosal alterations accompanied by an excessive desmoplastic reaction. Despite recent research on alternative therapies, surgical resection appears the only potentially curative approach. Patient selection and multidisciplinary management are paramount when considering surgical resection in patients with gastric LP. The operative approach in patients with LP has historically been questioned because of the poor outcomes. The aim of this review is to highlight different dimension of linitis plastica stomach in respect to its definition, classification, clinico-pathological characters, diagnostic approaches and treatment outcome. Journal of Surgical Sciences (2018) Vol. 22 (2) : 125-138

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SOOTY MOULD DISEASES OF SOME TREES FROM AURANGABAD DISTRICT, MAHARASHTRA (INDIA)

FLORA AND FAUNA ◽

10.33451/florafauna.v23i2pp351-354 ◽

2017 ◽

Vol 23 (2) ◽

Author(s):

NAVALSINGH J. TODAWAT

Keyword(s):

Surface Area ◽

Fungal Pathogens ◽

Leaf Surface ◽

Severity Of Disease ◽

Sooty Mould ◽

Leaf Surface Area

Sooty mould diseases of Tress from Aurangabad district were surveyed. During the survey of tress, 5 species were found infected by fungal pathogens causing sooty mould diseases. Disease is easily identifiable by the presence of a black, velvety growth covering the leaf surface area. The fungus produces mycelium which is superficial and dark grows on the flowers, leaf, stem and sometime on fruits also. The severity of disease depends on the honeydew secretions by insects. The diseases were found to be caused by 5 species of fungi viz. Capnodium anonae, C. ramosum, Capnodium sp., Meliola bangalorensis and Meliola ranganthii.

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Multiple diagnostic approaches to palpable breast mass

Journal of the Korean Radiological Society ◽

10.3348/jkrs.1985.21.6.923 ◽

1985 ◽

Vol 21 (6) ◽

pp. 923

Author(s):

S Y Chin ◽

K H Kim ◽

N M Moon ◽

Y K Kim ◽

J J Jang

Keyword(s):

Breast Mass ◽

Diagnostic Approaches ◽

Palpable Breast

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Interspecies Interactions: Effects On Virulence And Antimicrobial Susceptibility Of Bacterial And Fungal Pathogens

10.3389/978-2-88966-097-1 ◽

2020 ◽

Keyword(s):

Antimicrobial Susceptibility ◽

Fungal Pathogens ◽

Interspecies Interactions

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Clinical manifestations and diagnostic approaches to the histamine intolerance syndrome in children

SOVREMENNAYA PEDIATRIYA ◽

10.15574/sp.2017.81.104 ◽

2017 ◽

Vol 81 (1) ◽

pp. 104-109

Author(s):

Y.V. Marushko ◽

◽

E.D. Moskovenko ◽

Keyword(s):

Clinical Manifestations ◽

Histamine Intolerance ◽

Diagnostic Approaches

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Inflammatory disease of the womans reproductive system: modern trends in diagnosis and therapy (literature review)

GYNECOLOGY ◽

10.26442/20795696.2018.6.180072 ◽

2018 ◽

Vol 20 (6) ◽

pp. 35-41

Author(s):

T Yu Pestrikova ◽

I V Yurasov ◽

E A Yurasova

Keyword(s):

Genital Tract ◽

Inflammatory Diseases ◽

Diagnosis And Treatment ◽

Long Term Results ◽

Modern Approach ◽

Pelvic Organs ◽

Results Of Treatment ◽

Number Of Patients ◽

Diagnostic Approaches

Medical, social and economic relevance of inflammatory diseases of the woman's reproductive organs requires a very careful attitude to the diagnosis and treatment of this pathology. The number of patients with genital infections and inflammatory diseases of the pelvic organs can takes the first place in structure of gynecological morbidity, and is 60.4-65.0%, and this fact is not unique to Russia, but all over the world. Incidence rate of inflammatory diseases of the pelvic organs in the first decade of the twenty-first century is increased at 1.4 times in patients who are from 18 to 24 years old and at 1.8 times in patients aged 25-29 years. At the same time, the cost of diagnosis and treatment has increased, reaching 50-60% of the total cost of providing gynecological care for population. The inflammatory diseases of pelvic organs are a collective concept. It includes of various nosological forms. There are numerous contradictions in the views on diagnostic approaches and treatment tactics, the nature of screening and control over the long-term results of treatment, the etiological and pathogenetic significance of various microorganisms found in the genital tract in patients with inflammatory diseases of the pelvic organs. Currently, there are many opinions among specialists about diagnostic approaches and treatment tactics, the type of screening and monitoring the long-term results of treatment, the etiological and pathogenetic role of various microorganisms which can be found in the genital tract in patients with inflammatory diseases. This review presents the results of a modern approach to the diagnosis, management and rehabilitation of patients with inflammatory diseases of the pelvic organs.

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Diseases of the kumara crop

New Zealand Plant Protection ◽

10.30843/nzpp.2009.62.4845 ◽

2009 ◽

Vol 62 ◽

pp. 402-402

Author(s):

S.L. Lewthwaite ◽

P.J. Wright

Keyword(s):

Fungal Pathogens ◽

Ipomoea Batatas ◽

Mechanical Damage ◽

Soft Rot ◽

Economic Loss ◽

Root Surface ◽

Black Rot ◽

Rhizopus Stolonifer ◽

Ceratocystis Fimbriata ◽

Pink Rot

The predominant diseases of the commercial kumara (Ipomoea batatas) or sweetpotato crop are caused by fungal pathogens The field disease pink rot results from infection by the fungus Sclerotinia sclerotiorum Lesions form on vines but may spread down stems to the roots The widespread nature of this disease in sweetpotato appears peculiar to New Zealand Scurf is a disease caused by Monilochaetes infuscans which occurs in the field but may proliferate amongst stored roots The disease causes a superficial discolouration of the root surface which is mainly cosmetic but can also increase root water loss in storage Infection by Ceratocystis fimbriata produces a disease known as black rot The disease can be transmitted amongst plants at propagation but is particularly rampant amongst roots in storage This disease is readily transmitted and can cause severe economic loss Fusarium oxysporum causes surface rots in stored roots characterised by light to dark brown lesions that tend to be firm dry and superficial The lesions may be circular and centred on wounds caused by insects or mechanical damage at harvest Soft rot caused by Rhizopus stolonifer generally occurs in roots after they are washed and prepared for the market Fungal infection occurs through wounds or bruised tissue producing distinctive tufts of white fungal strands and black spores

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