scholarly journals Reappraisal of Immotthia in Dictyosporiaceae, Pleosporales: Introducing Immotthia bambusae sp. nov. and Pseudocyclothyriella clematidis comb. et gen. nov. Based on Morphology and Phylogeny

2021 ◽  
Vol 12 ◽  
Author(s):  
Hong-Bo Jiang ◽  
Rajesh Jeewon ◽  
Samantha C. Karunarathna ◽  
Chayanard Phukhamsakda ◽  
Mingkwan Doilom ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Phylogenetic Trees ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Single Species ◽  
Type Specimen ◽  
Morphological Identification ◽  
Ongoing Research ◽  
Dna Sequence Data

Immotthia is a poorly known genus, and currently, no DNA sequence data are available to ascertain its proper phylogenetic placement and evolutionary relationships with other bitunicate fungi. To date, there are only two species accepted in the genus. During our ongoing research study of bambusicolous fungi in southwest China and Thailand, a fungus associated with stromata of Hypoxylon sp. was found on dead bamboo culms in Loei Province, Thailand. Preliminary morphological identification revealed that the fungal collection belongs to Immotthia. A novel species, Immotthia bambusae, is introduced herein based on a comparison of morphological characteristics with the type specimen of I. hypoxylon (≡ Amphisphaeria hypoxylon Ellis and Everh.), a synonym of I. atrograna (Cooke and Ellis) M. E. Barr. Phylogenetic analyses of a concatenated ITS, LSU, SSU, and TEF1-α DNA sequence matrix showed that Immotthia belongs to Dictyosporiaceae, Pleosporales. Despite I. bambusae strains constituting a supported subclade, they are nested with the genus Pseudocoleophoma. Pseudocoleophoma clematidis is morphologically different from all other Pseudocoleophoma species, while its conidial characteristics are similar to Cyclothyriella. Multigene phylogenetic analyses showed that P. clematidis formed a clade basal to Immotthia, separated from Pseudocoleophoma with strong statistical support. Therefore, we introduce a monotypic genus, Pseudocyclothyriella Phukhams. and Phookamsak, gen. nov. to accommodate the single species, Pseudocyclothyriella clematidis (Phukhams. and K. D. Hyde) Phukhams. and Phookamsak, comb. nov. Detailed descriptions, color micrographs, and phylogenetic trees to show the placement of the new taxa are provided. In addition, an updated taxonomic treatment of the genera Immotthia and Pseudocyclothyriella is also provided based on the study of the type materials and phylogeny generated from DNA sequence data.

scholarly journals Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand

MycoKeys ◽  
2018 ◽  
Vol 33 ◽  
pp. 25-67 ◽  
Author(s):  
Saowaluck Tibpromma ◽  
Kevin D. Hyde ◽  
Jayarama D. Bhat ◽  
Peter E. Mortimer ◽  
Jianchu Xu ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Endophytic Fungi ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Taxonomic Status ◽  
Universal Primers ◽  
Specific Gene ◽  
Dna Sequence Data ◽  
Southern Thailand

The authors established the taxonomic status of endophytic fungi associated with leaves of Pandanaceae collected from southern Thailand. Morphotypes were initially identified based on their characteristics in culture and species level identification was done based on both morphological characteristics and phylogenetic analyses of DNA sequence data. Twenty-two isolates from healthy leaves were categorised into eight morphotypes. Appropriate universal primers were used to amplify specific gene regions and phylogenetic analyses were performed to identify these endophytes and established relationships with extant fungi. The authors identified both ascomycete and basidiomycete species, including one new genus, seven new species and nine known species. Morphological descriptions, colour plates and phylogenies are given for each taxon.

scholarly journals Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand

MycoKeys ◽  
2018 ◽  
Vol 33 ◽  
pp. 25-67 ◽  
Author(s):  
Saowaluck Tibpromma ◽  
Kevin D. Hyde ◽  
Jayarama D. Bhat ◽  
Peter E. Mortimer ◽  
Jianchu Xu ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Endophytic Fungi ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Taxonomic Status ◽  
Universal Primers ◽  
Specific Gene ◽  
Dna Sequence Data ◽  
Southern Thailand

The authors established the taxonomic status of endophytic fungi associated with leaves of Pandanaceae collected from southern Thailand. Morphotypes were initially identified based on their characteristics in culture and species level identification was done based on both morphological characteristics and phylogenetic analyses of DNA sequence data. Twenty-two isolates from healthy leaves were categorised into eight morphotypes. Appropriate universal primers were used to amplify specific gene regions and phylogenetic analyses were performed to identify these endophytes and established relationships with extant fungi. The authors identified both ascomycete and basidiomycete species, including one new genus, seven new species and nine known species. Morphological descriptions, colour plates and phylogenies are given for each taxon.

Two novel Acervus species extend their distribution within Yunnan, China

Phytotaxa ◽  
2016 ◽  
Vol 283 (1) ◽  
pp. 74 ◽  
Author(s):  
ANUSHA H. EKANAYAKA ◽  
QI ZHAO ◽  
GARETH E.B. JONES ◽  
EN-DA PU ◽  
KEVIN D. HYDE
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Yunnan Province ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Morphological Features ◽  
Dna Sequence Data ◽  
Two New Species ◽  
Small Genus

Acervus is a small genus in Pyronemataceae. Most of the species in this genus have been recorded from China. In the present study, two species of Acervus from Yunnan Province, in southwestern China, were investigated by using morphology and DNA sequence data. This paper introduces two new species A. stipitatus and A. globulosus, with morphological descriptions, and compares them with morphologically similar taxa. Multi-gene phylogenetic analyses inferred from 28S, TEF1 and RPB2 sequence data strongly support the lineages for taxa of Acervus, corresponding to morphological features. We also provide a summarized comparison of the important morphological characteristics of Acervus species.

Revision of Batrachospermum sections Acarposporophytum and Aristata (Batrachospermales, Rhodophyta) with the establishment of the new genera Acarposporophycos and Visia

Phytotaxa ◽  
2019 ◽  
Vol 395 (2) ◽  
pp. 51 ◽  
Author(s):  
ORLANDO NECCHI JR ◽  
AURO GARCIA FILHO ◽  
MONICA O. PAIANO
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Sequence Divergence ◽  
Morphological Evidence ◽  
Similar Species ◽  
Type Specimens ◽  
Generic Level ◽  
Dna Sequence Data ◽  
Cell Layers

Sections of the paraphyletic genus Batrachospermum have been recently investigated using molecular (DNA sequence data) and morphological evidence. Some sections have been recognized as distinct genera in order to resolve paraphyly. Batrachospermum species of sections Acarposporophytum and Aristata are showed to form well-supported clades and, in this study, we propose to raise them to the generic level, Acarposporophycos gen. nov. and Visia gen. nov., respectively. In addition, we re-evaluated the characters used to circumscribe species by reexamining type specimens as well as new collections. Acarposporophycos, with the sole species A. brasiliensis, is characterized by the lack of carposporophytic stage, with the fertilized carpogonia germinating to form directly the filaments of the ‘Chantransia’ stage on the gametophyte. Of the five previously accepted species in the genus Visia, we recognize four species: V. cayennensis—type species (synonym Batrachospermum beraense), V. cylindrocellularis, V. longiarticulata and V. turgida. The species are circumscribed on the basis of morphological characteristics (shape and cell layers of primary fascicles, occurrence of secondary fascicles, post-fertilization development of carpogonia) and DNA sequence data (rbcL and COI-5P). Specimens described as Visia cayennensis from Australia and Brazil had a high sequence divergence in comparison to those from or near the type locality (French Guiana), and are here referred to as Visia spp. Further studies are recommended to better discriminate these morphologically similar species on a broad geographical scale and define their status at the species level. Descriptions, identification key and photomicrographs are presented for each recognized species.

Molecular phylogenetics of the Onobrychis genus (Fabaceae: Papilionoideae) using ITS and trnL–trnF DNA sequence data

2014 ◽  
Vol 62 (3) ◽  
pp. 235 ◽  
Author(s):  
S. Safaei Chaei Kar ◽  
F. Ghanavati ◽  
M. R. Naghavi ◽  
H. Amirabadi-zade ◽  
R. Rabiee
Keyword(s):  
Dna Sequence ◽  
Molecular Phylogenetics ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Dna Sequence Data ◽  
Group I ◽  
Molecular Phylogenetic ◽  
Nuclear Its ◽  
Cultivated Species ◽  
Relationship Of

Onobrychis, comprising more than 130 species, is a genus of the family Fabaceae. At this time, the interspecies relationship of this biologically important genus is still a subject of great discussion and debate. To help resolve this disagreement, we used molecular phylogeny to analyse internal transcribed spacer (ITS) and trnL–trnF sequences of 76 species of Onobrychis. Bayesian interference, maximum parsimony and maximum likelihood analyses of nuclear ITS and plastid trnL–trnF DNA sequence data generated trees with strong posterior probability for two groups: Onobrychis subgen. Sisyrosema (including: Heliobrychis, Hymenobrychis, Afghanicae and Anthyllium sections) along with Laxiflorae section in Group I and Onobrychis subgen. Onobrychis (except Laxiflorae section) in the other (Group II). The Laxiflorae section roots back to the ancestral node for Sisyrosema subgen. O. viciifolia (cultivated species), which is closely associated with O. cyri var. cyri, suggesting that the latter may be a wild progenitor of O. viciifolia. The present study supported the paraphyly of subgenera Onobrychis and Sisyrosema. The study proposed the paraphyletic nature of the sections Onobrychis, Dendrobrychis, Heliobrychis and Hymenobrychis. Together with our molecular phylogenetic analyses we present a review of Onobrychis morphology and discuss and compare our results with those of earlier morphological and molecular phylogenetic analyses.

scholarly journals Veterinary Fusarioses within the United States

2016 ◽  
Vol 54 (11) ◽  
pp. 2813-2819 ◽  
Author(s):  
Kerry O'Donnell ◽  
Deanna A. Sutton ◽  
Nathan Wiederhold ◽  
Vincent A. R. G. Robert ◽  
Pedro W. Crous ◽  
...  
Keyword(s):  
United States ◽  
Dna Sequence ◽  
Species Complex ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Distinct Species ◽  
The United States ◽  
Content Type ◽  
Dna Sequence Data ◽  
Species Complexes

Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67Fusariumstrains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within theFusarium solanispecies complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme,Fusarium keratoplasticum, andFusariumsp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of theF. falciformestrains (12/15) were recovered from equine keratitis infections; however, strains ofF. keratoplasticumandFusariumsp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that theFusarium incarnatum-equisetispecies complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance withinFusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and theFusarium oxysporumspecies complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes.

Senecio imbaburensis, proposed name for Lasiocephalus sodiroi in the genus Senecio (Asteraceae)

Phytotaxa ◽  
2013 ◽  
Vol 152 (1) ◽  
pp. 59
Author(s):  
Karol Marhold ◽  
Petr Sklenář
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Plastid Dna ◽  
Dna Sequence Data ◽  
Number Of Species ◽  
The Andes

Lasiocephalus Willd. ex Schlechtendal (1818: 308), as traditionally circumscribed (e.g., by Cuatrecasas 1978, Dušková et al. 2010), is a neotropical genus of ca 25 species confined to the Andes and distributed from Venezuela to Bolivia. Nevertheless, recent studies by Pelser et al. (2007, 2010) have shown that based on phylogenetic analyses of nrITS and plastid DNA sequence data, species of the genus Lasiocephalus are deeply embedded in Senecio Linnaeus (1753: 866), and, consequently, should be transferred into this latter genus. In fact, a number of species of Lasiocephalus were originally described as Senecio or had been, at some point, transferred into Senecio so only few transfers are necessary.

Is Ronnbergia (Bromeliaceae, Bromelioideae) a geographically disjunct genus? Evidence from morphology and chloroplast DNA sequence data

Phytotaxa ◽  
2015 ◽  
Vol 219 (3) ◽  
pp. 261 ◽  
Author(s):  
Julian Aguirre-Santoro ◽  
Julio Betancur ◽  
Gregory K. Brown ◽  
Timothy M. Evans ◽  
Fabiano Salgueiro ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Dna Sequence ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Phylogenetic Reconstruction ◽  
Morphological Data ◽  
Phylogenetic Study ◽  
Geographic Ranges ◽  
Dna Sequence Data ◽  
Disjunct Genus

A phylogenetic study testing the monophyly of the geographically disjunct genus Ronnbergia (Bromeliaceae, Bromelioideae) is presented. The phylogenetic analyses were based on taxon sampling that included all but one species of Ronnbergia, and representative lineages across the subfamily Bromelioideae. Three chloroplast DNA sequence markers (matK, psbA-trnH, and trnL-trnF) and morphological data were used for the phylogenetic reconstruction. Both the molecular and morphological datasets supported the polyphyly of Ronnbergia, either independently or in combination. These findings suggest that the geographic disjunction of this genus is most likely a product of taxonomic misinterpretation. The results also indicate that the species currently circumscribed in Ronnbergia are closely related to species in the genus Aechmea with similar geographic ranges. The datasets do not have enough resolution power to reconstruct a deep phylogenetic history that involves all the species of Ronnbergia. Nevertheless, this study provides clues for future approaches that should focus on a larger species sampling and the use of multi-locus DNA sequence data.

A new and endangered species of Iochroma (Solanaceae) from the cloud forests of central Peru and its phylogenetic position in Iochrominae

Phytotaxa ◽  
2015 ◽  
Vol 227 (2) ◽  
pp. 147 ◽  
Author(s):  
Marco Antonio Cueva Manchego ◽  
STACEY DEWITT SMITH ◽  
SEGUNDO LEIVA GONZÁLES
Keyword(s):  
New Species ◽  
Endangered Species ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Phylogenetic Position ◽  
Cloud Forests ◽  
Dna Sequence Data ◽  
A New Species ◽  
Iucn Criteria

Iochroma amicorum M. Cueva, S. D. Sm. & S. Leiva (Solanaceae), a new species from cloud forests of Central Peru, is described and illustrated. Phylogenetic analyses of DNA sequence data suggest that Iochroma amicorum is closely related to I. nitidum, I. umbellatum, I. grandiflorum and I. tingoanum, which collectively form the “U” clade of Iochrominae. Certain morphological characteristics of calyx and corolla as well as the type of habitat appear to be common in species of this clade. Iochroma amicorum is easily distinguished from other members of the “U” clade by its campanulate or funnel-shaped corolla with short and slightly reflexed lobes, the presence of an expanded stapet at the point of filament insertion with short lateral extensions united to the corolla, and its globose usually white fruits with tinges of purple at the base. Distribution, phenology and are discussed together with the position of the new species in Iochrominae. Iochroma amicorum is assessed as Endangered according to IUCN criteria B1ab (i, iii)

scholarly journals Deep phylogeographic splits but no taxonomic structure in the disjointly distributed Draba pacheri (Brassicaceae), a subendemic of the Eastern Alps

2021 ◽  
Author(s):  
Erich Kucs ◽  
Peter Schönswetter ◽  
Gerald M. Schneeweiss
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Single Species ◽  
Eastern Alps ◽  
Phylogenetic Position ◽  
Taxonomic Structure ◽  
European Alps ◽  
Aflp Data ◽  
Dna Sequence Data ◽  
Mountain Habitats

AbstractDraba (Brassicaeae), a model group for diversification and evolution in Arctic and mountain habitats, is taxonomically challenging and many of its species are insufficiently investigated. One such species is D. pacheri, an endemic of the eastern European Alps and the western Carpathians (here presumably extinct). Several hypotheses exist with respect to the phylogenetic position and the taxonomy of this species, but none of these has ever been tested using molecular data. In this article we examine (i) DNA sequence data to assess the phylogenetic position of D. pacheri within the genus and (ii) AFLP fingerprint data as well as morphometric data to address whether this species can be divided taxonomically into species or subspecies. DNA sequence data firmly place D. pacheri within the Core Draba Group III, whose internal relationships are, however, insufficiently resolved to precisely identify the closest relative of D. pacheri. AFLP data identify several genetically divergent lineages corresponding to geographically distinct regions. Although these lineages are congruent with hypotheses distinguishing either two species (D. pacheri s. str., D. norica) or one species with several subspecies, the lack of clear morphological separation, both with respect to the entire set of traits and single presumably diagnostic characters such as trichome morphology, renders recognition of a single species D. pacheri, as suggested previously, the best taxonomic solution. The deep and geographically strongly structured splits of D. pacheri likely are the result of isolation in several Pleistocene refugia and warrant that conservation efforts should involve populations from each of the main geographic subgroups.

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