scholarly journals Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products

2021 ◽  
Vol 12 ◽  
Author(s):  
Honghai Zhang ◽  
Heng Zhang ◽  
Xing Qin ◽  
Xiaolu Wang ◽  
Yuan Wang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Animal Feed ◽  
Safety Concern ◽  
Ultra Performance Liquid Chromatography ◽  
Degradation Pathway ◽  
Intermediate Products ◽  
Liquid Chromatography Tandem Mass ◽  
Food And Feed ◽  
Level 3

Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, Nocardioides sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this strain were identified by high-performance liquid chromatography and ultra-performance liquid chromatography tandem mass spectrometry analyses. It was shown that, on an experimental level, 3-keto-DON was a necessary intermediate product during the conversion from DON to 3-epi-DON. Furthermore, the ZHH-013 strain could also utilize 3-epi-DON. This DON degradation pathway is a safety concern for food and feed. The mechanism of DON and 3-epi-DON elimination will be further studied, so that new enzymes for DON degradation can be identified.

Isolation and characterisation of enzymatic zearalenone hydrolysis reaction products

2016 ◽  
Vol 9 (3) ◽  
pp. 353-363 ◽  
Author(s):  
E. Vekiru ◽  
S. Fruhauf ◽  
C. Hametner ◽  
G. Schatzmayr ◽  
R. Krska ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Animal Feed ◽  
Fusarium Species ◽  
Reaction Products ◽  
Extraction Solvent ◽  
His Tag ◽  
Liquid Chromatography Tandem Mass ◽  
Unstable Intermediate ◽  
Primary Reaction Product

Zearalenone (ZEA) is an oestrogenic mycotoxin produced by several Fusarium species, and it frequently contaminates cereals used for food or animal feed. A ZEA-lactonase of Gliocladium roseum was previously described to hydrolyse ZEA to an unstable intermediate, which spontaneously decarboxylates to non-oestrogenic, decarboxylated hydrolysed ZEA (DHZEN). We expressed a codon-optimised version of the ZEA-lactonase (ZHD101) gene of G. roseum MA 918 with a secretion leader in Pichia pastoris and purified the recombinant enzyme from culture supernatant by His-tag mediated affinity chromatography. After incubation of the enzyme with ZEA, we detected the previously elusive primary reaction product hydrolysed ZEA (HZEN) by liquid chromatography tandem mass spectrometry, purified it by preparative high-performance liquid chromatography, and confirmed its postulated structure ((E)-2,4-dihydroxy-6-(10-hydroxy-6-oxo-1-undecen-1-yl)benzoic acid) by nuclear magnetic resonance techniques. Spontaneous decarboxylation to DHZEN ((E)-1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen-6-one), but not to a previously reported isomer, was observed. Biomass resuspensions of G. roseum strains MA 918 and the strains used for previous work, NBRC 7063 and ATCC 8684, all converted ZEA to HZEN, DHZEN, and further unknown metabolites. We studied partitioning of HZEN and DHZEN between aqueous phases and organic solvents, and found that HZEN did not partition into chloroform as extraction solvent, under the conditions used by previous authors. In contrast, extraction with ethyl acetate at pH 2.0 was suitable for simultaneous extraction of HZEN and DHZEN. The detection of HZEN and its availability as an analytical standard may assist further work towards possible application of ZEA-lactonase (e.g. determining kinetic parameters) for detoxification of ZEA.

scholarly journals VALIDATION OF 6-MERCAPTOPURINE AND 6-THIOGUANINE IN DRIED BLOOD SPOT BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

2018 ◽  
Vol 10 (1) ◽  
pp. 412
Author(s):  
Yahdiana Harahap ◽  
Cheputri Rahma Astrini ◽  
Herman Suryadi
Keyword(s):  
Liquid Chromatography ◽  
Formic Acid ◽  
High Performance ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Dried Blood Spot ◽  
European Medicines Agency ◽  
Tandem Mass ◽  
Liquid Chromatography Tandem Mass ◽  
Blood Spot

Objective: This study aimed to obtain an optimal and validated method of analyzing 6-mercaptopurine (6-MP) and 6-thioguanine (6-TG) simultaneouslyin dried blood spot samples using ultra high-performance liquid chromatography-tandem mass spectrometry.Method: Separation was performed with a 1.7-μm amide column, which had a mobile phase with a flow rate of 0.2 mL/min and comprised 0.2%formic acid in water, 0.1% formic acid in acetonitrile, and methanol with a gradient elution. Detection was performed using Waters Xevo TQD.Result: This method was linear with a range of 25–1000 ng/mL for 6-MP and 6-TG, with consecutive r values of ≥0.996 and ≥0.995, respectively. Theintra- and inter-day % difference value and coefficient of variation for the accuracy and precision were not more than 15% and 20%, respectively, ata concentration lower limit of quantitation.Conclusion: This method fulfilled the requirements of the European Medicines Agency guideline for validation.

scholarly journals Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of Elaeagnusmultiflora Thunb

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 849
Author(s):  
Sabina Lachowicz-Wiśniewska ◽  
Ireneusz Kapusta ◽  
Carla M. Stinco ◽  
Antonio J. Meléndez-Martínez ◽  
Anna Bieniek ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Photodiode Array Detection ◽  
Fruit Skin ◽  
Liquid Chromatography Tandem Mass

The purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of Elaeagnus multiflora Thunb., as well as their in vitro biological potency. The polyphenols and isoprenoids were determined with UPLC-PDA-MS/MS (ultra-performance liquid chromatography coupled to photodiode array detection and electrospray ionization tandem mass spectrometry) and RRLC-MS/MS (rapid resolution liquid chromatography/tandem mass spectrometry) methods, the organic acid with HPLC-RID (high-performance liquid chromatography coupled to a Refractive Index Detector), and the antioxidant capacity using ABTS and FRAP assays. Enzymatic activity was established as the ability to inhibit α-amylase, α-glucosidase, and pancreatic lipase. Owing to such an effective technique, 88 compounds were recorded, with 17 polyphenolic compounds and 3 isoprenoids identified for the first time in the seeds and leaves of cherry silverberry. In total, 55 compounds were identified in the leaves, 36 in the seeds, and 31 in the fruit skin + pulp. The predominant polyphenol was polymeric procyanidin (66–95% of total polyphenolics), whereas the predominant isoprenoids were chlorophyll b and (all-E)-lycopene. The results of our work noted that there are significant differences in the profiles of several secondary metabolites between the analyzed parts of the plant, and depending on the need, the compounds can be used to develop different innovative food or cosmetic products.

scholarly journals Rapid Determination of Fumonisins in Corn-Based Products by Liquid Chromatography/Tandem Mass Spectrometry

2010 ◽  
Vol 93 (5) ◽  
pp. 1472-1481 ◽  
Author(s):  
Wei Li ◽  
Timothy J Herrman ◽  
Susie Y Dai
Keyword(s):  
Liquid Chromatography ◽  
Animal Feed ◽  
Rapid Determination ◽  
Fumonisin B1 ◽  
Ultra Performance Liquid Chromatography ◽  
Sample Volume ◽  
High Throughput Analysis ◽  
Accuracy And Precision ◽  
Liquid Chromatography Tandem Mass

Abstract A simple, fast, and robust method was developed for the determination of fumonisin B1 (FB1), fumonisin B2 (FB2), and fumonisin B3 (FB3) in corn-based human food and animal feed (cornmeal). The method involves a single extraction step followed by centrifugation and filtration before analysis by ultra-performance liquid chromatography/electrospray ionization (UPLC/ESI)-MS/MS. The LC/MS/MS method developed here represents the fastest and simplest procedure (<30 min) among both conventional HPLC methods and other LC/MS methods using SPE cleanup. The potential for high throughput analysis makes the method particularly beneficial for regulatory agencies and analytical laboratories with a high sample volume. A single-laboratory validation was conducted by testing three different spiking levels (200, 500, and 1000 ng/g for FB1 and FB2; 100, 250, and 500 ng/g for FB3) for accuracy and precision. Recoveries of FB1 ranged from 93 to 98% with RSD values of 3-8%. Recoveries of FB2 ranged from 104 to 108, with RSD values of 2-6%. Recoveries of FB3 ranged from 94% to 108%, with RSD values of 2-5%.

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