scholarly journals Isolation, Characterization, and Evaluation of Native Rhizobacterial Consortia Developed From the Rhizosphere of Rice Grown in Organic State Sikkim, India, and Their Effect on Plant Growth

2021 ◽  
Vol 12 ◽  
Author(s):  
Mingma Thundu Sherpa ◽  
Laxuman Sharma ◽  
Niladri Bag ◽  
Sayak Das
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Burkholderia Cenocepacia ◽  
Growth And Yield ◽  
Root Weight ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Large Cardamom

Eight rhizospheric bacteria were isolated from the organic paddy fields of Sikkim, India, and identified as Pseudomonas kribbensis KSB, Burkholderia cenocepacia SRD, Kosakonia oryzendophytica YMA7, Pseudomonas rhodesiae SRB, Bacillus sp. ARA, Paenibacillus polymyxa COW3, Bacillus aryabhattai PSB2, and Bacillus megaterium PSB1. They showed plant growth-promoting attributes in rice and have bio-control potential against phytopathogen Colletotrichum gloeosporioides of large cardamom (Amomum subulatum). Burkholderia cenocepacia SRD showed production of indole acetic acid and ammonia and solubilization of phosphate and potassium and also possessed nitrogen fixation potential. It showed antagonistic activity against two other plant pathogens of large cardamom, viz., Curvularia eragrostidis and Pestalotiopsis sp., under in vitro conditions. The liquid bacterial consortium was prepared using the bacterial strains SRB, PSB1, and COW3 (Consortia-1); PSB2, SRD, and COW3 (Consortia-2); and COW3, KSB, and YMA7 (Consortia-3) to increase the growth and yield of rice plants under organic farming conditions. Greenhouse and field studies showed that the Consortia-3 had the highest plant growth-promoting activity. Consortia-3 demonstrated better agronomic performance in terms of root length (9.5 cm),number of leaflets per plant (5.3), grains per panicle (110.6), test grain weight (27.4 g), dry root weight per plant (0.73 g), and total dry biomass per plant (8.26 g).

scholarly journals Plant Growth Promotion and Biocontrol Properties of Aneurinibacillus migulanus Isolated from Maize Roots (Zea mays)

2021 ◽  
pp. 46-59
Author(s):  
Lynda Kelvin Asogwa ◽  
Frank C. Ogbo
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Plant Height ◽  
Pot Experiment ◽  
Root Weight ◽  
Dual Culture ◽  
Maize Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Aims: To isolate Plant Growth Promoting Bacillus strain from maize roots, to evaluate its biocontrol potentials and to characterize the isolate using16S rRNA sequencing. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, between February 2019 and March 2020. Methodology: The isolation of Plant Growth Promoting Rhizobacteria (PGPR) from maize roots was done using Pikovskaya (PVK) agar. Quantitative determination of phosphate was carried out using PVK broth. Evaluations of other plant growth promoting properties were carried out such as IAA, etc. Fusarium and Enterobacter plant pathogens were isolated from diseased maize plants. The in vitro antagonism effects of the PGPR isolates against the pathogens were analyzed using the dual culture plate technique. The pot experiment was carried out in a completely randomized design. Plant characteristics such as plant height, shoot  and root weight, chlorophyll content, as well as disease assessment were recorded accordingly. The organisms were identified using phenotypic and molecular methods. Results: Seven PGPR bacteria were isolated from maize (Zea mays) roots using PVK agar. Aneurinibacillus migulanus gave the highest solubilization index of 4.21 while isolate IS48 gave the lowest solubilization index of 1.47. A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide. The organism showed antagonism activity against the two tested phytopathogens. In the pot experiment, A. migulanus treated plants showed a statistically insignificant difference in maize plant height at P=0.05 but gave significant increases in shoot and root wet weights. The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment. Conclusion: A. migulanus solubilized phosphate in addition to other plant growth promoting  properties. It showed biocontrol potentials both in vitro and in vivo and thus can be used as substitute for synthetic agrochemicals.

scholarly journals Impact of Pseudomonas spp. on Plant Growth, Lytic Enzymes and Secondary Metabolites Production

2021 ◽  
Vol 3 ◽  
Author(s):  
Rajesh R. Waghunde ◽  
Ambalal N. Sabalpara
Keyword(s):  
Plant Growth ◽  
Acid Production ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Root Weight ◽  
Bacterial Strains ◽  
Lytic Enzymes ◽  
Acetic Acid Production ◽  
Metabolites Production

Seven strains of Pseudomonas spp. were isolated from the south Gujarat region of India. Antifungal and bacterial activities of bacterial strains were evaluated against important plant pathogens in vitro, among them, PaRS was found most effective. The indole acetic acid production was recorded in all isolated Pseudomonas spp. Seed treatment with PaRS at 6 g/kg was significantly superior over other treatments for plant growth promotion (germination, shoot and root length, shoot and root weight, vigor index, and both shoot and root colonization) under standard roll towel method and pot conditions. The maximum siderophore production was observed in PaRS and medium production in PfRB, PaNS, and PfNC. PaNS and PaRS strains recorded strong HCN production but moderate production recorded in PaWP, PaWS, and PfNC. The maximum phosphate solubalization zone (22 mm) was found in PaRS. PaRS recorded maximum chitinase, ß-1,3-glucanase activity, hydrogen cyanide, and salicylic acid production as compared to other strains.

scholarly journals Biocontrol of Macrophomina phaseolina (Tassi) Goid causing charcoal rot disease in Lycopersicon esculentum L. by using multi species bacterial consortia

2021 ◽  
Vol 22 (3) ◽  
pp. 441-449
Author(s):  
Pradeep Kumar ◽  
Sandeep Kumar ◽  
R.C. Dubey
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Growth Promotion ◽  
Macrophomina Phaseolina ◽  
Growth And Yield ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting bacterial strains (LEP1-LEP31) were isolated from rhizosphere of Lycopersicon esculentum L. (Tomato) and screened for their plant growth promoting (PGP) activities. On the basis of morphological, physiological, biochemical, carbon source utilization and molecular characterization, these strains were identified as Pseudomonas sp., Azotobacter sp. and Bacillus sp. For antagonistic activities all the strains were subject to the chitinase activities by the development of clear halo around the inoculated bacterial spots when loaded on chitin (0.2%) supplemented mediumBased on pot and field trial results of individual strains and consortium application, it may be concluded that all the three strains i.e. Pseudomonas sp. LEP17, Azotobacter sp. strain LEP21 and Bacillus sp. strain LEP25 showed plant growth promoting effects. The growth promotion provided by these strains was apparently related to improve shoot and root development, which resulted in better nutrient uptake capability and suppression of plant pathogen. All these three strains were superior in this regard because they provided the best and most consistent effects on growth and yield of L. esculentum. All these strains Pseudomonas sp. LEP17, Azotobacter sp. strain LEP21, Bacillus sp. strain LEP25 and their consortium seems to be suitable for use as a plant growth promoting and improvement of growth and yield

scholarly journals Investigation of Arsenic-Resistant, Arsenite-Oxidizing Bacteria for Plant Growth Promoting Traits Isolated From Arsenic Contaminated Soils

2021 ◽  
Author(s):  
Aritri Laha ◽  
Somnath Bhattacharyya ◽  
Sudip Sengupta ◽  
Kallol Bhattacharyya ◽  
Sanjoy GuhaRoy
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Burkholderia Cepacia ◽  
Growth Promotion ◽  
Burkholderia Cenocepacia ◽  
Nodule Formation ◽  
Stressed Condition ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract The menace of arsenic (As) pollution being severe warrants opting for low cost microbial remediation strategies. The present study of identifying suitable bacterial strains led to isolation of eleven As-tolerant strains from the As contaminated rhizosphere soils of West Bengal, India. They were found to oxidize/reduce 55%-31.6% of 5 mM As(III) and 73%-37.6% of 5 mM As(V) within 12 hrs. The four isolates (BcAl-1, JN 73, LAR-2 and AR-30) had high level of As(III) oxidase activity along with higher level of As(V) and As(III) resistance. The presence of aoxB gene was observed in these four As(III) oxidizing isolates. Evaluation of plant growth promoting characteristics revealed that BcAl-1 (Burkholderia cepacia), JN73 (Burkholderia metallica), AR-30 (Burkholderia cenocepacia) and LAR-2 (Burkholderia sp) had enormous plant growth-promoting characteristics (PGP), including ability to solubilize phosphate, siderophore production, indole acetic acid like molecules production, ACC deaminase production and nodule formation under As stressed condition. BcAl-1 and JN73 emerged as the most promising traits in As removal as well as plant growth promotion.

scholarly journals Seed treatment with chitosan synergizes plant growth promoting ability ofPseudomonas aeruginosa-P17 in sorghum (Sorhum bicolorL.)

2019 ◽  
Author(s):  
G. Praveen Kumar ◽  
Suseelendra Desai ◽  
Bruno M. Moerschbacher ◽  
Nour Eddine-El Gueddari
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Abiotic Stress Tolerance ◽  
Growth And Yield ◽  
Systemic Resistance ◽  
Bacterial Strains ◽  
Promoting Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractInoculation of crop plants with PGPR has in a large number of investigations resulted in increased plant growth and yield both in the greenhouse and in the field. This plant growth promoting effect of bacteria could be due to net result of synergistic effect of various pgpr traits that they exert in the rhizosphere region of the plant. Four (04) bacterial strain of fluorescent Pseudomonas spp. viz. P1, P17, P22 and P28 were identified previously for their plant growth promoting nature and abiotic stress tolerance and selected further to assess their chitinolytic activity and growth promotion on sorghum in combination with chitosans of low and high degree of acetylation. It was found that P1 has no chitin degrading nature and rest of the three strains have this property. When studied for their ability to grow in presence of chitosans of DA 1.6, 11, 35 and 56% all the strains showed growth in presence of chitosans. Seed bacterization of sorghum seeds with 04 bacterial strains in the presence and absence of chitosans (both low and high DA) and assessment of plant growth promotion after 15 days of sowing showed that P17+DA 56% chitosan combination showed higher growth of seedlings in plant growth chamber with highest root length of 25.9 cm, highest shoot length of 32.1 cm and dry mass of 132.7 mg/ plant. In P17+DA 56% chitosan treated seedlings various defence enzymes and PR-proteins were found to be present in highest quantities as compared to P1 and un-inoculated controls. Since this strain showed highest growth promotion of sorghum seedlings chitin-chitosan modifying enzyme (CCME) of this strain was partially characterized using different proteomic tools and techniques. CCME of P17 had one active polypeptide with a Pi in the range of 3.0-4.0. The digestion pattern of acetylated and deacetylated chitosans showed that P17 enzyme has endochitinase activity. Substrate specificity assay showed that the enzyme had more specificity towards highly acetylated chitosans. Two dimensional PAGE and MS analysis of the protein revealed similarities of this enzyme with protein of Pseudomonas aeruginosa chitinase PA01 strain of GenBank. In conclusion, the study established the option of opening new possibilities for developing bacterial-chitosan (P17+DA 56% chitosan) product for plant growth promotion and induced systemic resistance in sorghum.

scholarly journals Bioprospecting of elite plant growth-promoting bacteria for the maize crop

2020 ◽  
Vol 42 ◽  
pp. e44364 ◽  
Author(s):  
Angela Cristina Ikeda ◽  
Daiani Cristina Savi ◽  
Mariangela Hungria ◽  
Vanessa Kava ◽  
Chirlei Glienke ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Maize Crop ◽  
Agriculture Sustainability ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Biological Nitrogen

The use of plant growth-promoting bacteria (PGPB), which aims to replace chemical fertilizers and biological control, is a goal for achieving agriculture sustainability. In this scenario, our goal was to identify and evaluate the potential of bacteria isolated from maize roots to promote plant growth and be used as inoculants. We evaluated 173 bacterial strains isolated from the maize (Zea mays L.) rhizosphere for the properties of their PGPB in vitro. Twelve strains were positive for siderophores, indole acetic acid (IAA) production, biological nitrogen fixation (BNF), and phosphate solubilization. Sequence analysis of 16S rRNA identified these strains as belonging to the genera Cellulosimicrobium, Stenotrophomonas, Enterobacter, and Bacillus. The elite strains were evaluated under greenhouse conditions upon the inoculation of two maize hybrids, ATL100 and KWX628. The ability of the isolates to promote plant growth was dependent on the maize genotype; Enterobacter sp. LGMB208 showed the best ability to promote growth of hybrid ATL100, while Enterobacter sp. strains LGMB125, LGMB225, and LGMB274 and Cellulosimicrobium sp. strain LGMB239 showed the best ability to promote growth of hybrid KWX628. The results highlight the potential of bacterial genera little explored as maize PGPB but indicate the need to investigate their interactions with different plant genotypes.

scholarly journals In vitro and in planta antagonistic effects of plant growth-promoting rhizobacteria consortium against soilborne plant pathogens of Solanum tuberosum and Solanum lycopersicum

2020 ◽  
Vol 367 (13) ◽  
Author(s):  
Marika Pellegrini ◽  
Claudia Ercole ◽  
Chiara Di Zio ◽  
Federica Matteucci ◽  
Loretta Pace ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Plant Pathogens ◽  
Solanum Tuberosum L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Dual Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Potatoes (Solanum tuberosum L.) and tomatoes (Solanum lycopersicum L.), among the main crops belonging to the Solanaceae family, are attacked by several pathogens. Among them Fusarium oxysporum f. sp. radicis-lycopersici and Rhizoctonia solani are very common and cause significant losses. Four plant growth-promoting rhizobacteria, Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae and Burkholderia ambifaria were tested against these pathogens. In vitro antagonistic activities of single strains were assessed through dual culture plates. Strains showing antagonistic activity (G. diazotrophicus, H. seropedicae and B. ambifaria) were combined and, after an in vitro confirmation, the consortium was applied on S. lycopersicum and S. tuberosum in a greenhouse pot experiment. The bioprotection was assessed in pre-emergence (infection before germination) and post-emergence (infection after germination). The consortium was able to successfully counteract the infection of both F. oxysporum and R. solani, allowing a regular development of plants. The biocontrol of the fungal pathogens was highlighted both in pre-emergence and post-emergence conditions. This selected consortium could be a valid alternative to agrochemicals and could be exploited as biocontrol agent to counteract losses due to these pathogenic fungi.

scholarly journals Plant Growth-Promoting Rhizobacteria Isolated from Degraded Habitat Enhance Drought Tolerance of Acacia (Acacia abyssinica Hochst. ex Benth.) Seedlings

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Alemayehu Getahun ◽  
Diriba Muleta ◽  
Fassil Assefa ◽  
Solomon Kiros
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
In Vitro Assays ◽  
Plant Tolerance ◽  
Bacterial Strains ◽  
Root And Shoot Length ◽  
Plant Growth Promoting ◽  
Growth Promoting

Drought stress (DS) is the most impacting global phenomenon affecting the ecological balance of a particular habitat. The search for potential plant growth-promoting rhizobacteria (PGPR) capable of enhancing plant tolerance to drought stress is needed. Thus, this study was initiated to evaluate the effect of inoculating Acacia abyssinica seedlings with PGPR isolated from rhizosphere soil of Ethiopia to enhance DS tolerance. The strains were selected based on in vitro assays associated with tolerance to drought and other beneficial traits such as salinity, acidity, temperature, heavy metal tolerances, biofilm formation, and exopolysaccharide (EPS) production. The strains with the best DS tolerance ability were selected for the greenhouse trials with acacia plants. The results indicate that out of 73 strains, 10 (14%) were completely tolerant to 40% polyethylene glycol. Moreover, 37% of the strains were strong biofilm producers, while 66 (90.41%) were EPS producers with a better production in the medium containing sucrose at 28 ± 2°C and pH 7 ± 0.2. Strains PS-16 and RS-79 showed tolerance to 11% NaCl. All the strains were able to grow in wider ranges of pH (4–10) and temperature (15–45°C) and had high tolerance to heavy metals. The inoculated bacterial strains significantly ( p ≤ 0.05 ) increased root and shoot length and dry biomass of acacia plants. One of the strains identified as P. fluorescens strain FB-49 was outstanding in enhancing DS tolerance compared to the single inoculants and comparable to consortia. Stress-tolerant PGPR could be used to enhance acacia DS tolerance after testing other phytobeneficial traits.

scholarly journals Draft genome sequences of two Streptomyces albidoflavus strains DEF1AK and DEF147AK with plant growth-promoting and biocontrol potential

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Andrea Kunova ◽  
Paolo Cortesi ◽  
Marco Saracchi ◽  
Guy Migdal ◽  
Matias Pasquali
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Draft Genome ◽  
Gc Content ◽  
Mechanisms Of Action ◽  
Genome Sequences ◽  
Fungal Plant Pathogens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Purpose Bacteria belonging to the Streptomyces genus can be exploited in environmentally friendly approaches to food safety. Genome information can help to characterize bioactive strains opening the possibility to decipher their mechanisms of action. Methods The biocontrol and plant growth-promoting activity of two Streptomyces spp. strains, DEF1AK and DEF147AK, were assessed in vitro and in planta. The genome sequences were determined using the Illumina NextSeq sequencing system and were assembled using EvoCAT (Evogene Clustering and Assembly Toolbox). Result Streptomyces spp. DEF1AK and DEF147AK were able to improve seed germination and early plant development of maize, wheat, and tomato and inhibited the mycelium growth of diverse fungal plant pathogens in vitro. The genome sequence analysis identified both strains as S. albidoflavus (99% sequence identity). Both genomes were of 7.1-Mb length with an average GC content of 73.45%. AntiSMASH and MIBiG analyses revealed strain-specific sets of secondary metabolite gene clusters in the two strains as well as differences in the number and type of duplicated genes. Conclusion The combination of the biological activity and genomic data is the basis for in-depth studies aimed at the identification of secondary metabolites involved in plant growth-promoting and biocontrol activity of Streptomyces spp. The comparison of unique genomic features of the two strains will help to explain their diverse biocontrol and plant growth-promoting activities and warrant targeted functional genomics approaches to verify their mechanisms of action.

scholarly journals Impact of plant growth-promoting rhizobacteria on yield and disease control of Nicotiana tabacum

2018 ◽  
Vol 70 (4) ◽  
pp. 717-725
Author(s):  
Sohail Khan ◽  
Fazli Subhan ◽  
Kashif Haleem ◽  
Muhammad Khattak ◽  
Ibrar Khan ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Vitro Assay ◽  
Soil Microbial ◽  
Auxin Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

An unexplored soil microbial community associated with the root system of Nicotiana tabacum was isolated to analyze its impact on growth and yield of the crop. A total of nine isolates out of 180 were biochemically screened and characterized as potential plant growth-promoting rhizobacteria due to the expression of growth-promoting traits. All isolates were positive for ammonia production, 8 were positive for phosphate solubilization but none for auxin production. The majority of the isolates were also found positive for hydrogen cyanide, siderophore and hydrolytic/degradative enzymes production, enabling them to restrict the growth of Fusarium oxysporum in an in vitro assay. Although all tested isolates enhanced tobacco growth significantly, Baj-ER-01 and CD-RS-03 were found to be the most promising in enhancing all aspects of growth. This study provides evidence for the enhancement of growth and yield of inoculated tobacco plants through an adequate supply of nutrients and/or controlling phytopathogens.

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