scholarly journals In vitro and in planta antagonistic effects of plant growth-promoting rhizobacteria consortium against soilborne plant pathogens of Solanum tuberosum and Solanum lycopersicum

2020 ◽  
Vol 367 (13) ◽  
Author(s):  
Marika Pellegrini ◽  
Claudia Ercole ◽  
Chiara Di Zio ◽  
Federica Matteucci ◽  
Loretta Pace ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Plant Pathogens ◽  
Solanum Tuberosum L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Dual Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Potatoes (Solanum tuberosum L.) and tomatoes (Solanum lycopersicum L.), among the main crops belonging to the Solanaceae family, are attacked by several pathogens. Among them Fusarium oxysporum f. sp. radicis-lycopersici and Rhizoctonia solani are very common and cause significant losses. Four plant growth-promoting rhizobacteria, Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae and Burkholderia ambifaria were tested against these pathogens. In vitro antagonistic activities of single strains were assessed through dual culture plates. Strains showing antagonistic activity (G. diazotrophicus, H. seropedicae and B. ambifaria) were combined and, after an in vitro confirmation, the consortium was applied on S. lycopersicum and S. tuberosum in a greenhouse pot experiment. The bioprotection was assessed in pre-emergence (infection before germination) and post-emergence (infection after germination). The consortium was able to successfully counteract the infection of both F. oxysporum and R. solani, allowing a regular development of plants. The biocontrol of the fungal pathogens was highlighted both in pre-emergence and post-emergence conditions. This selected consortium could be a valid alternative to agrochemicals and could be exploited as biocontrol agent to counteract losses due to these pathogenic fungi.

scholarly journals Whole-Genome Sequences of Three Plant Growth-Promoting Rhizobacteria Isolated from Solanum tuberosum L. Rhizosphere in Tanzania

2020 ◽  
Vol 9 (20) ◽  
Author(s):  
Becky N. Aloo ◽  
Ernest R. Mbega ◽  
Billy A. Makumba ◽  
Ines Friedrich ◽  
Robert Hertel ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
Complete Genome ◽  
Solanum Tuberosum L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

We present here the complete genome sequences of plant growth-promoting Klebsiella sp. strain MPUS7, Serratia sp. strain NGAS9, and Citrobacter sp. strain LUTT5, isolated from rhizosphere soils and tubers of potato (Solanum tuberosum L.) plants growing in the northern and southern highlands of Tanzania.

scholarly journals Pseudomonas fluorescens Pf7: A Potential Biocontrol Agent against Aspergillus flavus Induced Aflatoxin Contamination in Groundnut

2019 ◽  
pp. 1-7 ◽  
Author(s):  
M. Ravi Teja ◽  
K. Vijay Krishna Kumar ◽  
H. Sudini
Keyword(s):  
Plant Growth ◽  
Aspergillus Flavus ◽  
Plant Growth Promoting Rhizobacteria ◽  
Aflatoxin Contamination ◽  
Dual Culture ◽  
Arachis Hypogaea L ◽  
Potential Biocontrol Agent ◽  
Plant Growth Promoting ◽  
Growth Promoting

Aflatoxin contamination is a qualitative problem in groundnut (Arachis hypogaea L.) occurring at both pre-and post-harvest stages. These aflatoxins are secondary metabolites produced by Aspergillus flavus and A. parasiticus and have carcinogenic, hepatotoxic, teratogenic and immuno-suppressive effects. Use of plant growth-promoting rhizobacteria (PGPR) is a viable and sustainable option in managing aflatoxin problem in groundnut. Our present study is aimed at identifying a plant growth-promoting rhizobacteria (PGPR) strain with superior antagonistic abilities on A. flavus infection, aflatoxin contamination and to determine its mode of action. Ten native P. fluorescens isolates were isolated from groundnut rhizosphere and screened against A. flavus by dual culture and in vitro seed colonization (IVSC) assays. In dual culture and IVSC studies, Pf7 exhibited higher degree of antagonism on A. flavus (54% inhibition), inhibited its colonization and reduced aflatoxin contamination (27.8 µg kg-1) in kernels.

scholarly journals Plant Growth Promotion and Biocontrol Properties of Aneurinibacillus migulanus Isolated from Maize Roots (Zea mays)

2021 ◽  
pp. 46-59
Author(s):  
Lynda Kelvin Asogwa ◽  
Frank C. Ogbo
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Plant Height ◽  
Pot Experiment ◽  
Root Weight ◽  
Dual Culture ◽  
Maize Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Aims: To isolate Plant Growth Promoting Bacillus strain from maize roots, to evaluate its biocontrol potentials and to characterize the isolate using16S rRNA sequencing. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, between February 2019 and March 2020. Methodology: The isolation of Plant Growth Promoting Rhizobacteria (PGPR) from maize roots was done using Pikovskaya (PVK) agar. Quantitative determination of phosphate was carried out using PVK broth. Evaluations of other plant growth promoting properties were carried out such as IAA, etc. Fusarium and Enterobacter plant pathogens were isolated from diseased maize plants. The in vitro antagonism effects of the PGPR isolates against the pathogens were analyzed using the dual culture plate technique. The pot experiment was carried out in a completely randomized design. Plant characteristics such as plant height, shoot  and root weight, chlorophyll content, as well as disease assessment were recorded accordingly. The organisms were identified using phenotypic and molecular methods. Results: Seven PGPR bacteria were isolated from maize (Zea mays) roots using PVK agar. Aneurinibacillus migulanus gave the highest solubilization index of 4.21 while isolate IS48 gave the lowest solubilization index of 1.47. A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide. The organism showed antagonism activity against the two tested phytopathogens. In the pot experiment, A. migulanus treated plants showed a statistically insignificant difference in maize plant height at P=0.05 but gave significant increases in shoot and root wet weights. The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment. Conclusion: A. migulanus solubilized phosphate in addition to other plant growth promoting  properties. It showed biocontrol potentials both in vitro and in vivo and thus can be used as substitute for synthetic agrochemicals.

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