Biocontrol of Macrophomina phaseolina (Tassi) Goid causing charcoal rot disease in Lycopersicon esculentum L. by using multi species bacterial consortia

Plant growth-promoting bacterial strains (LEP1-LEP31) were isolated from rhizosphere of Lycopersicon esculentum L. (Tomato) and screened for their plant growth promoting (PGP) activities. On the basis of morphological, physiological, biochemical, carbon source utilization and molecular characterization, these strains were identified as Pseudomonas sp., Azotobacter sp. and Bacillus sp. For antagonistic activities all the strains were subject to the chitinase activities by the development of clear halo around the inoculated bacterial spots when loaded on chitin (0.2%) supplemented mediumBased on pot and field trial results of individual strains and consortium application, it may be concluded that all the three strains i.e. Pseudomonas sp. LEP17, Azotobacter sp. strain LEP21 and Bacillus sp. strain LEP25 showed plant growth promoting effects. The growth promotion provided by these strains was apparently related to improve shoot and root development, which resulted in better nutrient uptake capability and suppression of plant pathogen. All these three strains were superior in this regard because they provided the best and most consistent effects on growth and yield of L. esculentum. All these strains Pseudomonas sp. LEP17, Azotobacter sp. strain LEP21, Bacillus sp. strain LEP25 and their consortium seems to be suitable for use as a plant growth promoting and improvement of growth and yield

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Seed treatment with chitosan synergizes plant growth promoting ability ofPseudomonas aeruginosa-P17 in sorghum (Sorhum bicolorL.)

10.1101/601328 ◽

2019 ◽

Author(s):

G. Praveen Kumar ◽

Suseelendra Desai ◽

Bruno M. Moerschbacher ◽

Nour Eddine-El Gueddari

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Abiotic Stress Tolerance ◽

Growth And Yield ◽

Systemic Resistance ◽

Bacterial Strains ◽

Promoting Effect ◽

Plant Growth Promoting ◽

Growth Promoting

AbstractInoculation of crop plants with PGPR has in a large number of investigations resulted in increased plant growth and yield both in the greenhouse and in the field. This plant growth promoting effect of bacteria could be due to net result of synergistic effect of various pgpr traits that they exert in the rhizosphere region of the plant. Four (04) bacterial strain of fluorescent Pseudomonas spp. viz. P1, P17, P22 and P28 were identified previously for their plant growth promoting nature and abiotic stress tolerance and selected further to assess their chitinolytic activity and growth promotion on sorghum in combination with chitosans of low and high degree of acetylation. It was found that P1 has no chitin degrading nature and rest of the three strains have this property. When studied for their ability to grow in presence of chitosans of DA 1.6, 11, 35 and 56% all the strains showed growth in presence of chitosans. Seed bacterization of sorghum seeds with 04 bacterial strains in the presence and absence of chitosans (both low and high DA) and assessment of plant growth promotion after 15 days of sowing showed that P17+DA 56% chitosan combination showed higher growth of seedlings in plant growth chamber with highest root length of 25.9 cm, highest shoot length of 32.1 cm and dry mass of 132.7 mg/ plant. In P17+DA 56% chitosan treated seedlings various defence enzymes and PR-proteins were found to be present in highest quantities as compared to P1 and un-inoculated controls. Since this strain showed highest growth promotion of sorghum seedlings chitin-chitosan modifying enzyme (CCME) of this strain was partially characterized using different proteomic tools and techniques. CCME of P17 had one active polypeptide with a Pi in the range of 3.0-4.0. The digestion pattern of acetylated and deacetylated chitosans showed that P17 enzyme has endochitinase activity. Substrate specificity assay showed that the enzyme had more specificity towards highly acetylated chitosans. Two dimensional PAGE and MS analysis of the protein revealed similarities of this enzyme with protein of Pseudomonas aeruginosa chitinase PA01 strain of GenBank. In conclusion, the study established the option of opening new possibilities for developing bacterial-chitosan (P17+DA 56% chitosan) product for plant growth promotion and induced systemic resistance in sorghum.

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Simultaneous Removal of Reactive Dyes and Hexavalent Chromium by a Metal Tolerant Pseudomonas sp. WS-D/183 Harboring Plant Growth Promoting Traits

International Journal of Agriculture and Biology ◽

10.17957/ijab/15.1282 ◽

2020 ◽

Vol 23 (02) ◽

pp. 241-252

Author(s):

Sabir Hussain

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Treatment Strategies ◽

Reactive Dyes ◽

Pseudomonas Sp ◽

Bacterial Strains ◽

Acetic Acid Production ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Plant Growth Promoting Traits

Textile industry is a continuous source of colored wastewater. This wastewater frequently used for irrigation purpose in many underdeveloped countries including Pakistan. In this study, we isolated the bacterial strains capable of decolorizing dyes and promote the plant growth. Hence to decolorize the reactive red 120 (RR120), the strain WS-D/183 was optimized following response surface methodology (RSM) based modeling approach. Moreover, strain WS-D/183 was also assessed for plant growth promoting characteristics. Results revealed that the strain WS-D/183 showed a good potential for decolorization of structurally diverse types of azo dyes on reaction with a mixture of heavy metal ions (Cr6+, Cd2+, Zn2+, Pb2+). This strain concurrently removed reactive dyes (100 mg L-1 ) and reduced Cr(VI). Results showed that each dye was decolorized up to 90% except reactive yellow-2 which was decolorized up to 57.4%. Furthermore, the bacterium reduced Cr(VI) by 41 to 95% along with concurrent decolorization of RR120. This bacterium was also found to carry plant growth promoting traits including inorganic phosphate solubilization (497.6 ± 14.8 µg mL-1 ) and indole-3-acetic acid production (21.07 ± 0.9 µg mL-1 ). A phytotoxicity evaluation study indicated that irrigation of mung bean [Vigna radiata (L.) Wilczek] with RR120, Cr(VI) and RR120+Cr(VI) contaminated waters treated with the strain WS-D/183 enhanced germination along with plumule and radical length of seedlings. Results suggested that Pseudomonas sp. WS-D/183 is a valuable addition to the bioresources, which can be used to devise textile wastewater treatment strategies as well as for integrated bioremediation and plant growth promotion in agricultural soils contaminated with textile wastewaters. © 2020 Friends Science Publishers

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The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽

10.3390/agronomy11050888 ◽

2021 ◽

Vol 11 (5) ◽

pp. 888

Author(s):

Giorgia Novello ◽

Patrizia Cesaro ◽

Elisa Bona ◽

Nadia Massa ◽

Fabio Gosetti ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Global Market ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Mineral Concentration ◽

Positive Effects ◽

Plant Growth Promoting ◽

Onion Cultivar ◽

Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

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Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

Plant Science Today ◽

10.14719/pst.1377 ◽

2021 ◽

Vol 8 (sp1) ◽

pp. 17-24

Author(s):

Tanvir Kaur ◽

Rubee Devi ◽

Divjot Kour ◽

Ashok Yadav ◽

Ajar Nath Yadav

Keyword(s):

Plant Growth ◽

Environmental Sustainability ◽

Growth Promotion ◽

Growth Parameters ◽

Sugar Content ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Hordeum Vulgare L ◽

Plant Growth Promoting ◽

Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

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Plant growth promoting endophyte Burkholderia contaminans NZ antagonizes phytopathogen Macrophomina phaseolina through melanin synthesis and pyrrolnitrin inhibition

PLoS ONE ◽

10.1371/journal.pone.0257863 ◽

2021 ◽

Vol 16 (9) ◽

pp. e0257863

Author(s):

Nazia R. Zaman ◽

Umar F. Chowdhury ◽

Rifath N. Reza ◽

Farhana T. Chowdhury ◽

Mrinmoy Sarker ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Genome Mining ◽

Gene Clusters ◽

Macrophomina Phaseolina ◽

Melanin Synthesis ◽

Burkholderia Contaminans ◽

Plant Growth Promoting ◽

Growth Promoting

The endophytic bacterium Burkholderia contaminans NZ was isolated from jute, which is an important fiber-producing plant. This bacterium exhibits significant growth promotion activity in in vivo pot experiments, and like other plant growth-promoting (PGP) bacteria fixes nitrogen, produces indole acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. B. contaminans NZ is considered to exert a promising growth inhibitory effect on Macrophomina phaseolina, a phytopathogen responsible for infecting hundreds of crops worldwide. This study aimed to identify the possibility of B. contaminans NZ as a safe biocontrol agent and assess its effectiveness in suppressing phytopathogenic fungi, especially M. phaseolina. Co-culture of M. phaseolina with B. contaminans NZ on both solid and liquid media revealed appreciable growth suppression of M. phaseolina and its chromogenic aberration in liquid culture. Genome mining of B. contaminans NZ using NaPDoS and antiSMASH revealed gene clusters that displayed 100% similarity for cytotoxic and antifungal substances, such as pyrrolnitrin. GC-MS analysis of B. contaminans NZ culture extracts revealed various bioactive compounds, including catechol; 9,10-dihydro-12’-hydroxy-2’-methyl-5’-(phenylmethyl)- ergotaman 3’,6’,18-trione; 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one; 1-(1,6-Dioxooctadecyl)- pyrrolidine; 9-Octadecenamide; and 2- methoxy- phenol. These compounds reportedly exhibit tyrosinase inhibitory, antifungal, and antibiotic activities. Using a more targeted approach, an RP-HPLC purified fraction was analyzed by LC-MS, confirming the existence of pyrrolnitrin in the B. contaminans NZ extract. Secondary metabolites, such as catechol and ergotaman, have been predicted to inhibit melanin synthesis in M. phaseolina. Thus, B. contaminans NZ appears to inhibit phytopathogens by apparently impairing melanin synthesis and other potential biochemical pathways, exhibiting considerable fungistatic activity.

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Root-specific camalexin biosynthesis controls the plant growth-promoting effects of multiple bacterial strains

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1818604116 ◽

2019 ◽

Vol 116 (31) ◽

pp. 15735-15744 ◽

Cited By ~ 25

Author(s):

Anna Koprivova ◽

Stefan Schuck ◽

Richard P. Jacoby ◽

Irene Klinkhammer ◽

Bastian Welter ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Bacterial Strains ◽

Natural Ecosystems ◽

Promoting Effect ◽

Gene Encoding ◽

A Genome ◽

Sulfatase Activity ◽

Plant Growth Promoting ◽

Growth Promoting

Plants in their natural ecosystems interact with numerous microorganisms, but how they influence their microbiota is still elusive. We observed that sulfatase activity in soil, which can be used as a measure of rhizosphere microbial activity, is differently affected by Arabidopsis accessions. Following a genome-wide association analysis of the variation in sulfatase activity we identified a candidate gene encoding an uncharacterized cytochrome P450, CYP71A27. Loss of this gene resulted in 2 different and independent microbiota-specific phenotypes: A lower sulfatase activity in the rhizosphere and a loss of plant growth-promoting effect by Pseudomonas sp. CH267. On the other hand, tolerance to leaf pathogens was not affected, which agreed with prevalent expression of CYP71A27 in the root vasculature. The phenotypes of cyp71A27 mutant were similar to those of cyp71A12 and cyp71A13, known mutants in synthesis of camalexin, a sulfur-containing indolic defense compound. Indeed, the cyp71A27 mutant accumulated less camalexin in the roots upon elicitation with silver nitrate or flagellin. Importantly, addition of camalexin complemented both the sulfatase activity and the loss of plant growth promotion by Pseudomonas sp. CH267. Two alleles of CYP71A27 were identified among Arabidopsis accessions, differing by a substitution of Glu373 by Gln, which correlated with the ability to induce camalexin synthesis and to gain fresh weight in response to Pseudomonas sp. CH267. Thus, CYP71A27 is an additional component in the camalexin synthesis pathway, contributing specifically to the control of plant microbe interactions in the root.

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Draft Genome Sequence of a Kale (Brassica oleracea L.) Root Endophyte, Pseudomonas sp. Strain C9

Genome Announcements ◽

10.1128/genomea.00163-17 ◽

2017 ◽

Vol 5 (15) ◽

Cited By ~ 2

Author(s):

Aurelie Laugraud ◽

Sandra Young ◽

Emily Gerard ◽

Maureen O’Callaghan ◽

Steven Wakelin

Keyword(s):

Biological Control ◽

Plant Growth ◽

Brassica Oleracea ◽

Growth Promotion ◽

Draft Genome ◽

Pseudomonas Sp ◽

Root Endophyte ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Brassica Oleracea L

ABSTRACT Pseudomonas sp. strain C9 is a plant growth–promoting bacterium isolated from the root tissue of Brassica oleracea L. grown in soil from Marlborough, New Zealand. Its draft genome of 6,350,161 bp contains genes associated with plant growth promotion and biological control.

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Isolation and Molecular Characterization of a Model Antagonistic Pseudomonas aeruginosa Divulging In Vitro Plant Growth Promoting Characteristics

BioMed Research International ◽

10.1155/2018/6147380 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 9

Author(s):

Bushra Uzair ◽

Rehana Kausar ◽

Syeda Asma Bano ◽

Sammer Fatima ◽

Malik Badshah ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Plant Growth ◽

Growth Promotion ◽

Gene Bank ◽

Accession Number ◽

Bacterial Strains ◽

Rhizopus Microsporus ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Gene Bank Accession

The use of microbial technologies in agriculture is currently expanding quite rapidly with the identification of new bacterial strains, which are more effective in promoting plant growth. In the present study 18 strains of Pseudomonas were isolated from soil sample of Balochistan coastline. Among isolated Pseudomonas strains four designated as SP19, SP22, PS24, and SP25 exhibited biocontrol activities against phytopathogenic fungi, that is, Rhizopus microsporus, Fusarium oxysporum, Aspergillus niger, Alternaria alternata, and Penicillium digitatum; PS24 identified as Pseudomonas aeruginosa by 16srRNA gene bank accession number EU081518 was selected on the basis of its antifungal activity to explore its potential as plant growth promotion. PS24 showed multiple plant growth promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA), siderophore, and HCN production. In order to determine the basis for antifungal properties, antibiotics were extracted from King B broth of PS24 and analyzed by TLC. Pyrrolnitrin antibiotic was detected in the culture of strain PS24. PS24 exhibited antifungal activities found to be positive for hydrogen cyanide synthase Hcn BC gene. Sequencing of gene of Hcn BC gene of strain PS24 revealed 99% homology with the Pseudomonas aeruginosa strain PA01. The sequence of PS24 had been submitted in gene bank accession number KR605499. Ps. aeruginosa PS24 with its multifunctional biocontrol possessions can be used to bioprotect the crop plants from phytopathogens.

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Enhancement of verticillium wilt resistance in tomato transplants by in vitro co-culture of seedlings with a plant growth promoting rhizobacterium (Pseudomonas sp. strain PsJN)

Canadian Journal of Microbiology ◽

10.1139/w98-017 ◽

1998 ◽

Vol 44 (6) ◽

pp. 528-536 ◽

Cited By ~ 67

Author(s):

V K Sharma ◽

J Nowak

Keyword(s):

Plant Growth ◽

Verticillium Wilt ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Disease Suppression ◽

Pseudomonas Sp ◽

Plant Growth Promoting ◽

Growth Promoting

The potential utilization of a plant growth promoting rhizobacterium, Pseudomonas sp. strain PsJN, to enhance the resistance of tomato transplants to verticillium wilt was investigated. Plant growth and disease development were tested on the disease-susceptible cultivar Bonny Best after Verticillium dahliae infection of tissue culture plantlets bacterized in vitro (by co-culturing with the bacterium) and seedlings bacterized in vivo (after 3 weeks growth in the greenhouse). Significant differences in both disease suppression and plant growth were obtained between in vitro bacterized and nonbacterized (control) plants. The degree of protection afforded by in vitro bacterization depended on the inoculum density of V. dahliae; the best and worst protection occurred at the lowest (103 conidia ·mL-1) and highest (106 conidia ·mL-1) levels, respectively. In contrast, the in vivo bacterized tomatoes did not show plant growth promotion when compared to the nonbacterized control plants. When challenged with Verticillium, significant growth differences between in vivo bacterized plants (26.8% for shoot height) and nonbacterized controls were only seen at the 3rd week after inoculation. Compared with the in vitro inoculation, there was no delay in the verticillium wilt symptom expression, even at the lowest concentration of V. dahliae, by in vivo PsJN inoculation. These results suggest that endophytic colonization of tomato tissues is required for the Verticillium-resistance responses. Plant growth promotion preceeds the disease-resistance responses and may depend on the colonization thresholds and subsequent sensitization of hosts.Key words: Pseudomonas sp., plant growth promoting rhizobacterium, Verticillium dahliae, tomato, colonization, plant growth promotion, disease suppression.

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Effect of Indigenous Pseudomonas sp. and Bacillus sp. Strains on Yield and Main Chemical Growth Parameters of Radicchio

Contemporary Agriculture ◽

10.2478/contagri-2018-0003 ◽

2018 ◽

Vol 67 (1) ◽

pp. 20-26

Author(s):

Aleksandra Stanojković-Sebić ◽

Radmila Pivić ◽

Zoran Dinić ◽

Renata Iličić ◽

Dragana Latković ◽

...

Keyword(s):

Plant Growth ◽

Growth Parameters ◽

Plant Growth Promoting Rhizobacteria ◽

Pseudomonas Sp ◽

Bacillus Sp ◽

Aerial Parts ◽

Dry Biomass ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Calcium And Magnesium

Summary Pseudomonas sp. and Bacillus sp. belong to plant growth promoting rhizobacteria which are able to colonize the plants roots and stimulate growth. In this study, the effect of two indigenous plant growth promoting rhizobacterial strains Pseudomonas sp. Q4 and Bacillus sp. Q10 and their mixture (mix Q4+Q10) on content of the main chemical growth parameters (nitrogen, phosphorus, potassium, calcium and magnesium) and the yield of dry biomass of radicchio (Cichorium spp. var. rossa di treviso) aerial parts and root, was investigated. The study was carried out with stagnosol type of soil in pot experiments under semi-controlled conditions in the Institute of Soil Science (Belgrade), in the period from July to October in 2013. Phosphorus was determined by spectrophotometer, potassium - by flame emission photometry and total nitrogen and carbon - using elemental CNS analyzer, while calcium and magnesium were determined by AAS. The data on yield of both aerial parts and root dry biomass of radicchio showed that its treatment with Q4 and Q10 strains, as well as with their mixture, caused noticeably increase in this parameter in relation to the control, whereby the strain Q4 was more effective for aerial parts, while mix Q4+Q10 - for roots. The obtained data on the studied chemical parameters of radicchio root and aerial parts were in total accordance with their yield. Concluding, studied strains have a potential in promoting the biomass yield and main chemical growth parameters of both aerial parts and root of radicchio.

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