scholarly journals Draft genome sequences of two Streptomyces albidoflavus strains DEF1AK and DEF147AK with plant growth-promoting and biocontrol potential

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Andrea Kunova ◽  
Paolo Cortesi ◽  
Marco Saracchi ◽  
Guy Migdal ◽  
Matias Pasquali
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Draft Genome ◽  
Gc Content ◽  
Mechanisms Of Action ◽  
Genome Sequences ◽  
Fungal Plant Pathogens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Purpose Bacteria belonging to the Streptomyces genus can be exploited in environmentally friendly approaches to food safety. Genome information can help to characterize bioactive strains opening the possibility to decipher their mechanisms of action. Methods The biocontrol and plant growth-promoting activity of two Streptomyces spp. strains, DEF1AK and DEF147AK, were assessed in vitro and in planta. The genome sequences were determined using the Illumina NextSeq sequencing system and were assembled using EvoCAT (Evogene Clustering and Assembly Toolbox). Result Streptomyces spp. DEF1AK and DEF147AK were able to improve seed germination and early plant development of maize, wheat, and tomato and inhibited the mycelium growth of diverse fungal plant pathogens in vitro. The genome sequence analysis identified both strains as S. albidoflavus (99% sequence identity). Both genomes were of 7.1-Mb length with an average GC content of 73.45%. AntiSMASH and MIBiG analyses revealed strain-specific sets of secondary metabolite gene clusters in the two strains as well as differences in the number and type of duplicated genes. Conclusion The combination of the biological activity and genomic data is the basis for in-depth studies aimed at the identification of secondary metabolites involved in plant growth-promoting and biocontrol activity of Streptomyces spp. The comparison of unique genomic features of the two strains will help to explain their diverse biocontrol and plant growth-promoting activities and warrant targeted functional genomics approaches to verify their mechanisms of action.

scholarly journals Draft Genome Sequence of the Plant Growth-Promoting Bacterium Pseudomonas pseudoalcaligenes KB-10

2021 ◽  
Vol 10 (20) ◽  
Author(s):  
M. M. A. Khan ◽  
Jin Duan ◽  
Bernard R. Glick ◽  
Patrick M. Finnegan ◽  
Saleh A. Kabli ◽  
...  
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Draft Genome Sequence ◽  
Circular Chromosome ◽  
Pseudomonas Pseudoalcaligenes ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Pseudomonas pseudoalcaligenes KB-10 can enhance salinity tolerance in coriander plants. We report a draft genome sequence of P. pseudoalcaligenes KB-10, comprising a 5,241,174-bp circular chromosome containing 4,921 genes, with a GC content of 62.97%.

scholarly journals Improved Draft Genome Sequence of Pseudomonas poae A2-S9, a Strain with Plant Growth-Promoting Activity

2019 ◽  
Vol 8 (15) ◽  
Author(s):  
Ye Xia ◽  
Seth DeBolt ◽  
Qin Ma ◽  
Adam McDermaid ◽  
Cankun Wang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Growth Promotion ◽  
Draft Genome ◽  
Gc Content ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting

We report here the improved draft genome sequence of Pseudomonas poae strain A2-S9, a bacterium that was originally isolated from switchgrass plants and exhibited the capacity for plant growth promotion. Its genome has a size of 6.68 Mbp and a GC content of 61.3%.

scholarly journals De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248274
Author(s):  
Iliana Julieta Cortese ◽  
María Lorena Castrillo ◽  
Andrea Liliana Onetto ◽  
Gustavo Ángel Bich ◽  
Pedro Darío Zapata ◽  
...  
Keyword(s):  
Plant Growth ◽  
De Novo ◽  
Draft Genome ◽  
Ilex Paraguariensis ◽  
Plant Growth Promoting Bacteria ◽  
De Novo Genome Assembly ◽  
Bacillus Altitudinis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting bacteria (PGPB) are a heterogeneous group of bacteria that can exert beneficial effects on plant growth directly or indirectly by different mechanisms. PGPB-based inoculant formulation has been used to replace chemical fertilizers and pesticides. In our previous studies, two endophytic endospore-forming bacteria identified as Bacillus altitudinis were isolated from roots of Ilex paraguariensis St. Hil. seedlings and selected for their plant growth-promoting (PGP) properties shown in vitro and in vivo. The purposes of this work were to assemble the genomes of B. altitudinis 19RS3 and T5S-T4, using different assemblers available for Windows and Linux and to select the best assembly for each strain. Both genomes were also automatically annotated to detect PGP genes and compare sequences with other genomes reported. Library construction and draft genome sequencing were performed by Macrogen services. Raw reads were filtered using the Trimmomatic tool. Genomes were assembled using SPAdes, ABySS, Velvet, and SOAPdenovo2 assemblers for Linux, and Geneious and CLC Genomics Workbench assemblers for Windows. Assembly evaluation was done by the QUAST tool. The parameters evaluated were the number of contigs ≥ 500 bp and ≥ 1000 bp, the length of the longest contig, and the N50 value. For genome annotation PROKKA, RAST, and KAAS tools were used. The best assembly for both genomes was obtained using Velvet. The B. altitudinis 19RS3 genome was assembled into 15 contigs with an N50 value of 1,943,801 bp. The B. altitudinis T5S-T4 genome was assembled into 24 contigs with an N50 of 344,151 bp. Both genomes comprise several genes related to PGP mechanisms, such as those for nitrogen fixation, iron metabolism, phosphate metabolism, and auxin biosynthesis. The results obtained offer the basis for a better understanding of B. altitudinis 19RS3 and T5S-T4 and make them promissory for bioinoculant development.

scholarly journals Isolation, Characterization, and Evaluation of Native Rhizobacterial Consortia Developed From the Rhizosphere of Rice Grown in Organic State Sikkim, India, and Their Effect on Plant Growth

2021 ◽  
Vol 12 ◽  
Author(s):  
Mingma Thundu Sherpa ◽  
Laxuman Sharma ◽  
Niladri Bag ◽  
Sayak Das
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Burkholderia Cenocepacia ◽  
Growth And Yield ◽  
Root Weight ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Large Cardamom

Eight rhizospheric bacteria were isolated from the organic paddy fields of Sikkim, India, and identified as Pseudomonas kribbensis KSB, Burkholderia cenocepacia SRD, Kosakonia oryzendophytica YMA7, Pseudomonas rhodesiae SRB, Bacillus sp. ARA, Paenibacillus polymyxa COW3, Bacillus aryabhattai PSB2, and Bacillus megaterium PSB1. They showed plant growth-promoting attributes in rice and have bio-control potential against phytopathogen Colletotrichum gloeosporioides of large cardamom (Amomum subulatum). Burkholderia cenocepacia SRD showed production of indole acetic acid and ammonia and solubilization of phosphate and potassium and also possessed nitrogen fixation potential. It showed antagonistic activity against two other plant pathogens of large cardamom, viz., Curvularia eragrostidis and Pestalotiopsis sp., under in vitro conditions. The liquid bacterial consortium was prepared using the bacterial strains SRB, PSB1, and COW3 (Consortia-1); PSB2, SRD, and COW3 (Consortia-2); and COW3, KSB, and YMA7 (Consortia-3) to increase the growth and yield of rice plants under organic farming conditions. Greenhouse and field studies showed that the Consortia-3 had the highest plant growth-promoting activity. Consortia-3 demonstrated better agronomic performance in terms of root length (9.5 cm),number of leaflets per plant (5.3), grains per panicle (110.6), test grain weight (27.4 g), dry root weight per plant (0.73 g), and total dry biomass per plant (8.26 g).

scholarly journals Improved Draft Genome Sequence of Bacillus sp. Strain YF23, Which Has Plant Growth-Promoting Activity

2019 ◽  
Vol 8 (15) ◽  
Author(s):  
Ye Xia ◽  
Seth DeBolt ◽  
Qin Ma ◽  
Adam McDermaid ◽  
Cankun Wang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Panicum Virgatum ◽  
Draft Genome ◽  
Gc Content ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting

We report here the improved draft genome sequence of Bacillus sp. strain YF23, a bacterium originally isolated from switchgrass (Panicum virgatum) plants and shown to exhibit plant growth-promoting activity. The genome comprised 5.82 Mbp, containing 5,933 genes, with 193 as RNA genes, and a GC content of 35.10%.

scholarly journals Draft Genome Sequence of Plant Growth-Promoting Rhizobacterium Burkholderia sp. Strain USMB20, Isolated from Nodules of Mucuna bracteata

2021 ◽  
Vol 10 (11) ◽  
Author(s):  
Nurohaida Ab Aziz ◽  
Salwani Shaffie ◽  
Ahmad Yamin Abdul Rahman ◽  
Yam Hokchai ◽  
Nazalan Najimudin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Cover Crop ◽  
Draft Genome ◽  
Gc Content ◽  
Draft Genome Sequence ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Burkholderia sp. strain USMB20 is a plant growth-promoting rhizobacterium that was isolated from nodules of the leguminous cover crop Mucuna bracteata. The draft genome sequence of Burkholderia sp. strain USMB20 has an assembly size of 7.7 Mbp in 26 contigs with a GC content of 66.88%.

scholarly journals Draft Genome Sequences of Pseudomonas koreensis Strain UASWS1668, Bacillus megaterium Strain UASWS1667, and Paenibacillus sp. Strain UASWS1643, Considered Potential Plant Growth-Promoting Rhizobacteria

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Julien Crovadore ◽  
Bastien Cochard ◽  
Romain Chablais ◽  
Martine Haenzi ◽  
François Raffini ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Megaterium ◽  
Draft Genome ◽  
Plant Growth Promoting Rhizobacteria ◽  
Horse Chestnut ◽  
Genome Sequences ◽  
Content Type ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Plant growth-promoting rhizobacteria (PGPR) include species in the genera Bacillus, Paenibacillus, and Pseudomonas. We report here the draft genome sequences of the strains Pseudomonas koreensis UASWS1668 and Bacillus megaterium UASWS1667, isolated from a horse chestnut tree, and Paenibacillus sp. strain UASWS1643, isolated from a tomato stem. Auxin production and phosphate solubilization were biochemically confirmed.

scholarly journals In vitro and in planta antagonistic effects of plant growth-promoting rhizobacteria consortium against soilborne plant pathogens of Solanum tuberosum and Solanum lycopersicum

2020 ◽  
Vol 367 (13) ◽  
Author(s):  
Marika Pellegrini ◽  
Claudia Ercole ◽  
Chiara Di Zio ◽  
Federica Matteucci ◽  
Loretta Pace ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Plant Pathogens ◽  
Solanum Tuberosum L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Dual Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Potatoes (Solanum tuberosum L.) and tomatoes (Solanum lycopersicum L.), among the main crops belonging to the Solanaceae family, are attacked by several pathogens. Among them Fusarium oxysporum f. sp. radicis-lycopersici and Rhizoctonia solani are very common and cause significant losses. Four plant growth-promoting rhizobacteria, Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae and Burkholderia ambifaria were tested against these pathogens. In vitro antagonistic activities of single strains were assessed through dual culture plates. Strains showing antagonistic activity (G. diazotrophicus, H. seropedicae and B. ambifaria) were combined and, after an in vitro confirmation, the consortium was applied on S. lycopersicum and S. tuberosum in a greenhouse pot experiment. The bioprotection was assessed in pre-emergence (infection before germination) and post-emergence (infection after germination). The consortium was able to successfully counteract the infection of both F. oxysporum and R. solani, allowing a regular development of plants. The biocontrol of the fungal pathogens was highlighted both in pre-emergence and post-emergence conditions. This selected consortium could be a valid alternative to agrochemicals and could be exploited as biocontrol agent to counteract losses due to these pathogenic fungi.

scholarly journals KARAKTERISASI DAN UJI AKTIVITAS BAKTERI RIZOSFER LAHAN ULTISOL SEBAGAI PEMACU PERTUMBUHAN TANAMAN DAN AGENSIA HAYATI CENDAWAN PATOGEN TULAR TANAH SECARA IN VITRO

2010 ◽  
Vol 10 (2) ◽  
pp. 123-130
Author(s):  
Andi Khaeruni ◽  
Gusti Ayu Kade Sutariati ◽  
Sri Wahyuni
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Plant Pathogens ◽  
Nitrogenase Activity ◽  
In Vitro Test ◽  
Biological Control Agents ◽  
Plant Growth Promoting ◽  
Vitro Test ◽  
Growth Promoting

Characterization and activities assay of rhizosphere bacteria from ultisol land for plant-growth promoting and biocontrol agents of soil-borne fungus pathogens under in vitro test.  Although many studies have been conducted to identify the specific traits of the plant growth-promoting and bioprotecting rhizobacteria (PGPBR), they were limited to studying specific PGPBR isolates from ultisol lands.  We selected 273 isolates from bulk soil and plant rhizosphere and examined them for a wide array traits that might inhibit the growth of plant pathogens and increase early cucumber growth in ultisol soil.  A subsample of 25 isolates, all positively produce chitinase and sellulose enzymes, 18 positively produce protease and 7 were fluorescens on KB medium under UV lighting.  All isolates could produce IAA and be able to solubilize phosphor in vitro test, 10 exhibited low level of nitrogenase activity. Futher test showed that out of 25 isolates, 12  inhibited F. oxysporum, P. capsici, R. solani and S. rolfsii in vitro.  All isolates increased seed germination, but only 5 isolates significanty increased early cucumber growth in ultisol soil. The results suggest that rhizobacteria be able to produce extracelluler enzymes, siderophore, ACC deasiminase, and IAA or those which are able to solubilize phosphor in vitro may be potential to be uses as biofertilizer and biological control agents in ultisol land.

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