scholarly journals Transcriptomic Analysis of Listeria monocytogenes in Response to Bile Under Aerobic and Anaerobic Conditions

2021 ◽  
Vol 12 ◽  
Author(s):  
Damayanti Chakravarty ◽  
Gyan Sahukhal ◽  
Mark Arick ◽  
Morgan L. Davis ◽  
Janet R. Donaldson
Keyword(s):  
Listeria Monocytogenes ◽  
Stress Responses ◽  
Listeriolysin O ◽  
Gi Tract ◽  
Anaerobic Conditions ◽  
Transcript Levels ◽  
Comprehensive Information ◽  
Associated Proteins ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Listeria monocytogenes is a gram-positive facultative anaerobic bacterium that causes the foodborne illness listeriosis. The pathogenesis of this bacterium depends on its survival in anaerobic, acidic, and bile conditions encountered throughout the gastrointestinal (GI) tract. This transcriptomics study was conducted to analyze the differences in transcript levels produced under conditions mimicking the GI tract. Changes in transcript levels were analyzed using RNA isolated from L. monocytogenes strain F2365 at both aerobic and anaerobic conditions, upon exposure to 0 and 1% bile at acidic and neutral pH. Transcripts corresponding to genes responsible for pathogenesis, cell wall associated proteins, DNA repair, transcription factors, and stress responses had variations in levels under the conditions tested. Upon exposure to anaerobiosis in acidic conditions, there were variations in the transcript levels for the virulence factors internalins, listeriolysin O, etc., as well as many histidine sensory kinases. These data indicate that the response to anaerobiosis differentially influences the transcription of several genes related to the survival of L. monocytogenes under acidic and bile conditions. Though further research is needed to decipher the role of oxygen in pathogenesis of L. monocytogenes, these data provide comprehensive information on how this pathogen responds to the GI tract.

scholarly journals Influence of pH on bile sensitivity amongst various strains of Listeria monocytogenes under aerobic and anaerobic conditions

2015 ◽  
Vol 64 (11) ◽  
pp. 1287-1296 ◽  
Author(s):  
Sally J. White ◽  
Daniel M. McClung ◽  
Jessica G. Wilson ◽  
Brandy N. Roberts ◽  
Janet R. Donaldson
Keyword(s):  
Listeria Monocytogenes ◽  
Anaerobic Conditions ◽  
Influence Of Ph ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Listeriolysin O Secretion by Listeria monocytogenes in Broth Containing Salts of Organic Acids

1995 ◽  
Vol 58 (12) ◽  
pp. 1314-1319 ◽  
Author(s):  
Y. KOUASSI ◽  
L. A. SHELEF
Keyword(s):  
Listeria Monocytogenes ◽  
Organic Acids ◽  
Blocking Agent ◽  
Sodium Lactate ◽  
Potassium Sorbate ◽  
Listeriolysin O ◽  
Anaerobic Incubation ◽  
Anaerobic Conditions ◽  
Salts Of Organic Acids ◽  
Aerobic And Anaerobic

Antilisterial effects of salts of organic acids have been documented, but there is little information on listeriolysin O (LLO) secretion in the presence of these salts during aerobic and anaerobic incubation. LLO secretion and populations of Listeria monocytogenes were studied in broth containing potassium sorbate (0.05 to 4%), sodium lactate, citrate, acetate, or propionate (0.1 to 8%) after aerobic or anaerobic incubation for 24 h at 35 and 20°C. The order of the antilisterial effects of the salts was propionate > sorbate > acetate > lactate > citrate. Cell proliferation was suppressed during incubation under anaerobic conditions but LLO secretion was enhanced. Citrate, acetate, and lactate enhanced LLO secretion during incubation at 35°C, whereas sorbate suppressed it. Overall, effects of the acids at 20°C were similar to those observed at 35°C, but only acetate and citrate enhanced LLO secretion. The observation that salts of specific organic acids enhance LLO secretion may suggest increased virulence of L. monocytogenes. Combinations of sorbate, an LLO blocking agent, with propionate or lactate, which inhibit proliferation of L. monocytogenes, may prolong the shelf life and increase the safety of foods.

Oxygen concentration affects frequency and range of transconjugants for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1

2020 ◽  
Vol 85 (4) ◽  
pp. 1005-1015
Author(s):  
Kentaro Ochi ◽  
Maho Tokuda ◽  
Kosuke Yanagiya ◽  
Chiho Suzuki-Minakuchi ◽  
Hideaki Nojiri ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Anaerobic Condition ◽  
Environmental Samples ◽  
Aerobic Condition ◽  
Recipient Strain ◽  
Donor Strain ◽  
Anaerobic Conditions ◽  
Filter Mating ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the transconjugant ranges under aerobic and anaerobic conditions, conjugation was performed between the donor of pBP136 and recipient bacteria extracted from environmental samples. Several transconjugants were uniquely obtained from each aerobic or anaerobic condition. Our findings indicate that a plasmid can differently spread among bacteria depending on the oxygen concentrations of the environment.

PRODUCTION AND PROPERTIES OF 2,3-BUTANEDIOL: VII. FERMENTATION OF WHEAT BY AEROBACILLUS POLYMYXA UNDER AEROBIC AND ANAEROBIC CONDITIONS

1946 ◽  
Vol 24f (1) ◽  
pp. 1-11 ◽  
Author(s):  
G. A. Adams
Keyword(s):  
Carbon Dioxide ◽  
Anaerobic Fermentation ◽  
Anaerobic Conditions ◽  
Mechanical Agitation ◽  
Fermentation Time ◽  
Aerobic Conditions ◽  
Ethanol Formation ◽  
Time Required ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Aeration by mechanical agitation of 15% wheat mash fermented by Aerobacillus polymyxa inhibited the formation of 2,3-butanediol and particularly of ethanol. Aeration of similar mashes by passage of finely dispersed air or oxygen at the rate of 333 ml. per minute per litre of mash increased the rate of formation and yield of 2,3-butanediol but inhibited ethanol formation. However, the over-all time required for the completion of fermentation was not shortened from the usual 72 to 96 hr. required for unaerated mashes. There was no evidence of a shift from fermentative to oxidative dissimilation. Under aerobic conditions, the final butanediol–ethanol ratio was approximately 3:1. Anaerobic conditions, as produced by the passage of nitrogen or hydrogen through the mash, increased the rate of formation of both butanediol and ethanol and shortened the fermentation time to about 48 hr. Under these conditions, the butanediol–ethanol ratio was reduced to about 1.3:1.0. Carbon dioxide gave a butanediol–ethanol ratio resembling that of anaerobic fermentation but did not reduce fermentation time.

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