scholarly journals Lipocalin-2 Regulates Hippocampal Microglial Activation in Poststroke Depression

2021 ◽  
Vol 13 ◽  
Author(s):  
Li Wei ◽  
Yupeng Du ◽  
Yirui Xie ◽  
Xiaopeng Yu ◽  
Hui Chen ◽  
...  
Keyword(s):  
Open Field ◽  
P38 Mapk ◽  
Field Test ◽  
Protein Level ◽  
Microglial Activation ◽  
Open Field Test ◽  
Mrna Level ◽  
The Other ◽  
Poststroke Depression ◽  
Hippocampal Region

Background and Purpose: Microglia play important role in poststroke depression (PSD), however, the exact mechanism was still unclear. The purpose of the study was to study the mechanism of microglial activation in PSD.Methods: 24 rats were randomly divided into three groups: the PSD group (n = 10), the poststroke (PS) group (n = 7), and the sham group (n = 7). Primary hippocampal microglia were isolated and cultured, and recombined LCN2 protein was used to stimulate the cultured microglia. The protein expression of Iba1, P38 MAPK and PP38 MAPK was analyzed by western blotting; the LCN2 expression was measured by RT-qPCR, the serum LCN2 level and the NO level were analyzed by ELISA.Results: Open field test scores (horizontal score, vertical score, and self-grooming score) and the serum LCN2 level were significantly decreased in the PSD group compared with the other two groups (P < 0.05). The serum LCN2 level was positively correlated with the horizontal score and negatively correlated with the self-grooming score in the open field test (P < 0.05). The relative protein level of Iba1 and the LCN2 mRNA level were significantly increased in the hippocampal region compared with other brain regions (P < 0.05), while the relative protein level of Iba1 and the LCN2 mRNA level were significantly increased in the PSD group compared with the other two groups (P < 0.05). The length, supernatant NO level, phagocytic ability and migration ability of LCN2-treated microglia were significantly increased compared with those of untreated microglia (P < 0.05). The relative protein levels of P38 MAPK and the PP38 MAPK significantly increased in hippocampal region in the PSD group and LCN2-treated hippocampal microglia (P < 0.05).Conclusion: Hippocampal microglia are activated during PSD; LCN2 may regulate hippocampal microglial activation by the P38 MAPK pathway in the process of PSD.

scholarly journals 189 What’s in a step? Evaluating the interrelationship between productivity and cattle behavior during a series of temperament tests

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 65-66
Author(s):  
Courtney L Daigle ◽  
Amanda J Hubbard ◽  
Xandra Meneses ◽  
Temple Grandin
Keyword(s):  
Open Field ◽  
Field Test ◽  
Washout Period ◽  
Positive Relationship ◽  
Open Field Test ◽  
Bos Indicus ◽  
Random Order ◽  
The Other ◽  
Multiple Components ◽  
Pearson's Correlation

Abstract To better understand the relationships among productivity, behavior, and emotion, in the context of Panksepps’ emotional circuits (FEAR, RAGE, PANIC, SEEKING), Bos indicus influence yearling steer behavior (n = 32) was quantified during a series of temperament evaluations. Exit velocities (EV) and chute scores (CS) were recorded at weaning. Starting three months post-weaning, ADG was measured across a 28-d period prior to testing commencement. Pen Scores (PS) from all steers were taken at the end of the 28-d period. Steers were video recorded during an Open Field Test (OFT), an Individual Startle Test (IST), a Group Startle Test (GST), and during a Bovine Zero Maze (BZM) evaluations, and the number of steps performed during each test recorded. Steers experienced each test twice, in random order, across a 50 day period with a 5-day minimum washout period between tests. Repeatability (Pearson’s correlation; PROC CORR), validity (relative to ADG; PROC REG), and reliability (Chronbach’s alpha; PROC CORR) were evaluated for PS, EV, CS, and the number of steps performed in the OFT, IST, GST, and BZM. Correlations among ADG, PS, EV, and CS illustrated that as ADG increased, EV (FEAR; R = -0.41; P = 0.02) and PS (PANIC; R = -0.42; P = 0.02) decreased. No relationship was observed between ADG and CS (RAGE). A positive relationship (R = 0.45; P = 0.01) was observed between PS (PANIC) and EV (FEAR). No relationship was observed between CS (RAGE) and EV nor between CS (RAGE) and PS (PANIC). The data separated (PROC VARCLUS) into the three primary clusters (FEAR, RAGE, and PANIC/SEEKING). The number of steps in the GST (FEAR) clustered with ADG and EV (FEAR). The number of steps in the BZM (PANIC/SEEKING), OFT (PANIC/SEEKING), and IST (PANIC/SEEKING) clustered together. Chute score (RAGE) did not cluster with any of the other variables. A model was developed to illustrate these relationships. CS, PS, and EV may be straightforward approaches to evaluating multiple components of temperament.

scholarly journals Toxicity and Neuropharmacological Effects of Elenine

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Eduardo Navarro ◽  
S. J. Alonso ◽  
R. Navarro
Keyword(s):  
Open Field ◽  
Field Test ◽  
Chemical Structure ◽  
Open Field Test ◽  
Control Group ◽  
General Behaviour ◽  
Similar Chemical ◽  
Sedative Effects ◽  
Traction Test ◽  
Central Depressant

Elenine is the aglycone of elenoside, a cytotoxic arylnaphthalene lignan (NSC 644013-W/1) derived fromJusticia hyssopifolia. (Family: Acanthaceae). Elenoside is a β-D-glucoside, with a similar chemical structure to etoposide, exhibiting central depressant activity. In the present study, elenine was given to mice and rats at doses of 10, 20, and 40 mg/kg. Acute toxicity (24 h) and general behaviour in mice was studied as well as its effects on muscular relaxant activity, locomotor activity (Varimex test), and the open-field test and were compared with 10 mg/kg of chlorpromazine. Elenine produced a reduction in the permanence time in muscular relaxant activity (traction test). Spontaneous activity was lower in the Varimex test. The ambulation and rearing were lower compared with the control group, and an increase in boluses was observed in the open-field test. Thus, it can be concluded that elenine has central sedative effects at lower doses than those used with elenoside and has a possible application in conditions of anxiety.

scholarly journals Participation ofGABAAChloride Channels in the Anxiolytic-Like Effects of a Fatty Acid Mixture

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Juan Francisco Rodríguez-Landa ◽  
Rosa Isela García-Ríos ◽  
Jonathan Cueto-Escobedo ◽  
Blandina Bernal-Morales ◽  
Carlos M. Contreras
Keyword(s):  
Open Field ◽  
Field Test ◽  
Chloride Channels ◽  
Wistar Rats ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Acid Mixture ◽  
Plus Maze ◽  
Gaba Binding ◽  
Defensive Burying

Human amniotic fluid and a mixture of eight fatty acids (FAT-M) identified in this maternal fluid (C12:0, lauric acid, 0.9 μg%; C14:0, myristic acid, 6.9 μg%; C16:0, palmitic acid, 35.3 μg%; C16:1, palmitoleic acid, 16.4 μg%; C18:0, stearic acid, 8.5 μg%; C18:1cis, oleic acid, 18.4 μg%; C18:1trans, elaidic acid, 3.5 μg%; C18:2, linoleic acid, 10.1 μg%) produce anxiolytic-like effects that are comparable to diazepam in Wistar rats, suggesting the involvement ofγ-aminobutyric acid-A (GABAA) receptors, a possibility not yet explored. Wistar rats were subjected to the defensive burying test, elevated plus maze, and open field test. In different groups, threeGABAAreceptor antagonists were administered 30 min before FAT-M administration, including the competitive GABA binding antagonist bicuculline (1 mg/kg),GABAAbenzodiazepine antagonist flumazenil (5 mg/kg), and noncompetitiveGABAAchloride channel antagonist picrotoxin (1 mg/kg). The FAT-M exerted anxiolytic-like effects in the defensive burying test and elevated plus maze, without affecting locomotor activity in the open field test. TheGABAAantagonists alone did not produce significant changes in the behavioral tests. Picrotoxin but not bicuculline or flumazenil blocked the anxiolytic-like effect of the FAT-M. Based on the specific blocking action of picrotoxin on the effects of the FAT-M, we conclude that the FAT-M exerted its anxiolytic-like effects throughGABAAreceptor chloride channels.

Acute effects of ethanol on behavioral responses of male and female zebrafish in the open field test with the influence of a non-familiar object

2021 ◽  
pp. 104474
Author(s):  
Thiele P. Souza ◽  
Francini Franscescon ◽  
Flavia V. Stefanello ◽  
Talise E. Müller ◽  
Laura W. Santos ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Behavioral Responses ◽  
Familiar Object ◽  
Acute Effects ◽  
Male And Female ◽  
Female Zebrafish

Garcinia Camobogia Extract Alters Anxiety, Sociability, and Dopamine Turnover in Male Swiss Albino Mice

2021 ◽  
Author(s):  
Michael K. Ibrahim ◽  
Marina Aboelsaad ◽  
Fatma Tony ◽  
Moustafa Sayed
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Modern Medicine ◽  
Receptor Expression ◽  
Dopamine Turnover ◽  
Male Mice ◽  
Garcinia Cambogia ◽  
Swiss Albino Mice ◽  
Albino Mice

Abstract Background: Obesity is a global concern, closely allied with somatic and psychosomatic disorders. Herbal drugs are available in modern medicine to treat obesity. Garcinia camobogia being used by so many people trying to lose weight produces various systemic side effects. The study was conducted to assess its effect on anxiety, sociability, and dopamine turnover in male mice. Methodology: Male Swiss albino mice of either were divided into three groups with seven mice in each group. Different groups were given distilled water (0.5ml p.o.) and Garcinia cambogia extract at two different doses (100mg/kg and 500 mg/kg p.o.). Effect of test drugs on anxiety was evaluated using open field test. Sociability and social novelty were evaluated using three chambers test. Results (mean ± standard deviation) were analyzed using one-way ANOVA test followed by Tukey’s test. Result: Garcinia cambogia extract significantly increased the time spent in the corners in the open field test, significantly reduced sociability and social novelty in the three chamber test, significantly reduced dopamine turnover and increased D2 receptor expression in ventral tegmental area. Conclusion: Garcinia cambogia extract have significant anxiogenic effect along with reduced sociability and social novelty in male mice. Moreover, these effects could be related to the altered dopamine turnover and D2 receptor expression in mice brain.

Early deprivation increases high-leaning behavior, a novel anxiety-like behavior, in the open field test in rats

2017 ◽  
Vol 123 ◽  
pp. 27-35 ◽  
Author(s):  
Hiroshi Kuniishi ◽  
Satoshi Ichisaka ◽  
Miki Yamamoto ◽  
Natsuko Ikubo ◽  
Sae Matsuda ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Early Deprivation

scholarly journals A comparative analysis of anxiolytic activity of Arnica montana and alprazolam in rats using open field test

2018 ◽  
Vol 7 (4) ◽  
pp. 718
Author(s):  
Puja Jha ◽  
Seema Bhalerao ◽  
Mrunal Dhole
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Physical Dependence ◽  
Forced Swim Test ◽  
Anxiolytic Activity ◽  
Combination Group ◽  
Control Group ◽  
Arnica Montana ◽  
Group I

Background: Anxiety affects around 7.3% of the total population worldwide. Benzodiazepines are preferred anxiolytic agents and are still frequently used in spite of the side effect profile including muscle relaxation, memory disturbances, sedation, physical dependence. Arnica montana, a traditional herb is known to possess significant anxiolytic effect at the dose of 100mg/kg. In this study, Arnica montana has been compared for the first time with alprazolam, a most commonly used anxiolytic drug.Methods: Forced swim test was used to induce anxiety. Anxiolytic action of study drugs which were given orally, was evaluated using Open field test (OFT) in healthy wistar rats of either sex. Behavior of rats, locomotion and number of squares crossed was recorded. Rats were divided into four groups with eight rats in each group. Study groups were Group I Control; Group II Alprazolam 0.08mg/kg; Group III Arnica montana extract (AME) 100mg/kg; Group IV AME + Alprazolam group 100mg/kg+0.08mg/kg. Statistical analysis was done using ANOVA followed by Tukey’s test (p<0.05).Results: Increase in frequency of rearing was significant (p<0.05) in AME group and highly significant (p<0.001) in Alprazolam and combination group in comparison to control. Decrease in frequency of grooming was highly significant (p<0.001) in Alprazolam and combination group. AME also showed significant (p<0.05) decrease in grooming activity.Conclusions: Arnica montana extract showed anxiolytic activity and can be used as an add on drug after further studies and validation in the treatment of anxiety disorders.

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