scholarly journals Eliminating Contamination in Umbilical Cord Blood Culture Sampling for Early-Onset Neonatal Sepsis

2021 ◽  
Vol 9 ◽  
Author(s):  
Vilmaris Quinones Cardona ◽  
Vanessa Lowery ◽  
David Cooperberg ◽  
Endla K. Anday ◽  
Alison J. Carey
Keyword(s):  
Quality Improvement ◽  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Peripheral Blood ◽  
Early Onset ◽  
Clinical Sepsis ◽  
Early Onset Sepsis ◽  
Antibiotic Duration

Introduction: Despite the advantages of umbilical cord blood culture (UCBC) use for diagnosis of early onset sepsis (EOS), contamination rates have deterred neonatologists from its widespread use. We aimed to implement UCBC collection in a level III neonatal intensive care unit (NICU) and apply quality improvement (QI) methods to reduce contamination in the diagnosis of early onset sepsis.Methods: Single-center implementation study utilizing quality improvement methodology to achieve 0% contamination rate in UCBC samples using the Plan-Do-Study-Act (PDSA) model for improvement. UCBC was obtained in conjunction with peripheral blood cultures (PBC) in neonates admitted to the NICU due to maternal chorioamnionitis. Maternal and neonatal characteristics between clinical sepsis and asymptomatic groups were compared. Process, outcome, and balancing measures were monitored.Results: Eighty-two UCBC samples were collected in addition to peripheral blood culture from neonates admitted due to maternal chorioamnionitis. Ten (12%) neonates had a diagnosis of clinical sepsis. All PBCs were negative and 5 UCBCs were positive in the study period. After 2 PDSA cycles, there was special cause variation with improvement in the percent of contaminated samples from 7.3 to 0%. There was no change in antibiotic duration among asymptomatic neonates.Conclusions: Implementation of UCBC for the diagnosis of EOS in term infants is feasible and contamination can be minimized with the implementation of a core team of trained providers and a proper sterile technique without increasing antibiotic duration.

scholarly journals Study of umbilical cord blood culture in diagnosis of early-onset sepsis among newborns with high-risk factors

2013 ◽  
Vol 2 (4) ◽  
pp. 169 ◽  
Author(s):  
MitulBabubhai Kalathia ◽  
IlaMitulkumar Kalathia ◽  
PrakashAshokbhai Shingala ◽  
ParinNiranjanbhai Parmar ◽  
YogeshNarenedrabhai Parikh
Keyword(s):  
Risk Factors ◽  
High Risk ◽  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
High Risk Factors ◽  
Early Onset Sepsis

Umbilical cord blood culture in neonatal early-onset sepsis: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Thomas H. Dierikx ◽  
Anton H. L. C. van Kaam ◽  
Tim G. J. de Meij ◽  
Ralph de Vries ◽  
Wes Onland ◽  
...  
Keyword(s):  
Systematic Review ◽  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
Meta Analysis ◽  
Early Onset Sepsis

Umbilical Cord Blood Culture in Diagnosis of Early Onset Neonatal Sepsis

2020 ◽  
Vol 87 (10) ◽  
pp. 793-797 ◽  
Author(s):  
Ramraj Meena ◽  
Kailash Kumar Meena ◽  
Vivek Athwani ◽  
Sunil Gothwal ◽  
Ghan Shyam Bairwa ◽  
...  
Keyword(s):  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Sepsis ◽  
Early Onset

scholarly journals The Correlation of Antibacterial Peptides Concentration in Umbilical Cord Blood and Early-Onset Sepsis in Preterm Infants

2021 ◽  
Author(s):  
Jiayu Miao ◽  
Ying Liu ◽  
Zengqing Li ◽  
Zhuxiao Ren ◽  
Zhicheng Zhong ◽  
...  
Keyword(s):  
Cord Blood ◽  
Preterm Infants ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
Sepsis Group ◽  
Control Group ◽  
Early Onset Sepsis ◽  
Prediction Probability

Abstract Background: The role of serum LL37 in systemic inflammation has been confirmed, and the influence of it in umbilical cord blood to early-onset sepsis in preterm infants is currently being investigated.Results: The level of LL37 of sepsis group was higher than those of in control group (362.13±46.71 vs 248.13±83.30 ng/ml), the levels of CRP, WBC and MPV in sepsis group were higher than those of in control group (6.25±4.19 vs 2.89±2.77 mg/L; 17.60±12.35 vs 8.24±3.55×109/L; 11.10±1.11 vs 8.93±0.68 fL), the level of PLT was lower than those of in control group (175.20±38.51 vs 245.75±49.85×109/L) (P < 0.05). The expression of LL37 was negatively correlated with PLT (r = -0.9347, P < 0.0001), and positively correlated with MPV ((r =0.9463 , P < 0.0001), the expression of PLT was negatively correlated with MPV (r = -0.9641, P < 0.0001). The area under curve of LL37 for diagnosis of early-onset sepsis was 0.875, the prediction probability was 0.7, the sensitivity was 90.0% and the specificity was 80.0%.Conclusions：The higher level of LL37 in umbilical cord blood was associated with the development of early-onset sepsis in preterm infants.

scholarly journals Use of umbilical cord blood culture in the diagnosis of early onset neonatal sepsis among high risk mothers

2021 ◽  
Vol 12 (12) ◽  
pp. 78-84
Author(s):  
Maitreyi Ojha ◽  
Ashish Pradhan ◽  
Sudip Dutta ◽  
Anamika Jaiswal
Keyword(s):  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Sepsis ◽  
Predictive Value ◽  
Early Onset ◽  
Venous Blood ◽  
Peripheral Vein ◽  
Peripheral Venous Blood

Background: Early onset neonatal sepsis (EONS) is one of the important causes of morbidity and mortality in neonates. Its early diagnosis and prompt treatment is essential and any delay in the diagnosis can have serious consequences including neonatal death. Blood culture is the gold standard test for diagnosis of neonatal sepsis. Umbilical cord blood culture (UCBC) is a painless procedure and technically less challenging. We conducted this study to evaluate use of UCBC for the diagnosis of EONS and compared it with the results of peripheral venous blood culture (PVBC) reports. Aims and Objectives: The aim of the study was to evaluate UCBC for the diagnosis of EONS and compared it with the results of PVBC reports. Materials and Methods: This was a hospital-based prospective cohort study consisting of 100 neonates who were at risk of EONS. The study was conducted in the Department of Pediatrics Sikkim Manipal Institute of Medical Sciences Gangtok between January 2018 and December 2019. Neonates found to be at risk of development of EONS were included in this study on the basis of a predefined inclusion and exclusion criteria. Immediately after birth blood samples were collected from both umbilical cord and peripheral vein and were sent to bacteriology lab. Sensitivity, specificity, positive predictive value, and negative predictive value of both the samples were analyzed. Results: Out of 100 neonates in 32 (32%) EONS could be confirmed with positive sepsis screening results and/or demonstration of organisms on blood culture. Among the 32 neonates with EONS, 17 were found to be premature. The mean gestational age of newborns with EONS was found to be 35.2 weeks. The umbilical blood culture was found to have sensitivity and specificity of 100% and 74.4%, respectively, whereas peripheral vein blood culture was found to have sensitivity and specificity of 77.7% and 72.5%, respectively. The most common organism grown in our study was Escherichia coli. Conclusion: UCBC is painless and technically less challenging method of blood sampling. It has been found to have a higher sensitivity as well specificity for the diagnosis of EONS as compared to peripheral venous blood sample.

scholarly journals Potential Impact of Umbilical-Cord-Blood Procalcitonin-Based Algorithm on Antibiotics Exposure in Neonates With Suspected Early-Onset Sepsis

2020 ◽  
Vol 8 ◽  
Author(s):  
Noémie Huetz ◽  
Elise Launay ◽  
Géraldine Gascoin ◽  
Bertrand Leboucher ◽  
Christophe Savagner ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
Early Onset Sepsis ◽  
Potential Impact

scholarly journals Umbilical Cord Procalcitonin to Detect Early-Onset Sepsis in Newborns: A Promising Biomarker

2021 ◽  
Vol 9 ◽  
Author(s):  
O. R. E. Dongen ◽  
L. M. van Leeuwen ◽  
P. K. de Groot ◽  
K. Vollebregt ◽  
I. Schiering ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cord Blood ◽  
Antibiotic Treatment ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
Antibiotic Administration ◽  
Reactive Protein ◽  
Early Onset Sepsis ◽  
Reliable Marker

Background: Up to 7% of neonates born in high-income countries receive antibiotics for suspected early-onset sepsis (EOS). Culture-proven neonatal sepsis has a prevalence of 0.2%, suggesting considerable overtreatment. We studied the diagnostic accuracy of umbilical cord blood and infant blood procalcitonin (PCT) in diagnosing EOS to improve antibiotic stewardship.Methods: Umbilical cord blood PCT was tested in newborns ≥ 32 weeks of gestation. Groups were defined as following: A) culture-proven or probable EOS (n = 25); B) Possible EOS, based on risk factors for which antibiotics were administered for &lt;72 h (n = 49); C) Risk factor(s) for EOS without need for antibiotic treatment (n = 181); D) Healthy controls (n = 74). Additionally, venous or capillary blood PCT and C-reactive protein (CRP) were tested if blood drawing was necessary for standard care.Results: Between June 2019 and March 2021, 329 newborns were included. Umbilical cord blood PCT was significantly higher in group A than in group C and D. No difference between venous or arterial samples was found. Sensitivity and specificity for cord blood procalcitonin were 83 and 62%, respectively (cut-off 0.1 ng/mL). Antepartum maternal antibiotic administration was associated with decreased PCT levels in both cord blood and infant blood directly postpartum in all groups combined.Conclusion: Umbilical cord blood PCT levels are increased in newborns ≥32 weeks with a proven or probable EOS and low in newborns with risk factors for infection, but PCT seems not a reliable marker after maternal antibiotic treatment. PCT could be useful to distinguish infected from healthy newborns with or without EOS risk factors.

scholarly journals PB2387 CD56 BRIGHT/CD16- NATURAL KILLER CELLS COUNTS ARE HIGHER IN THE UMBILICAL CORD BLOOD THAN IN THE PERIPHERAL BLOOD FROM ADULTS

HemaSphere ◽  
2019 ◽  
Vol 3 (S1) ◽  
pp. 1062
Author(s):  
M. Barbosa ◽  
V. Molla ◽  
A. Mendroni Junior ◽  
M. Goncalves ◽  
E. Kimura ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Cord Blood ◽  
Natural Killer ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Peripheral Blood ◽  
Killer Cells
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