Metabolomics Analysis of L-Arginine Induced Gastrointestinal Motility Disorder in Rats Using UPLC-MS After Magnolol Treatment

Abstract Background: Hawthorn, a commonly-used traditional Chinese medicine (TCM) for treating dyspepsia，dysmenorrhea and hyperlipidemia, etc., has been proven to improve gastrointestinal motility, avoid food retention. Due to its complex ingredients, the active fractions responsible for the treatment of improving digestion remain largely unknown. To explore the underlying material and interpret its potential mechanism, the therapeutic effect of extract from different polar parts of hawthorn on gastrointestinal motility disorder was studied based on the ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabolomics . Materials and Methods: The rat model of gastrointestinal motility disorder was established by subcutaneous injecting with atropine. The modeled rats were then treated with 4 polar parts (T1-4 in descending polarity, corresponding to water, n-butanol, ethyl acetate and petroleum ether extracts, respectively) of hawthorn for 5 consecutive days. The stomach, small intestine, plasma samples were collected and then subjected to related measurement (gastric emptying rate and small intestine propulsion rate), UPLC-MS/MS metabolic profiling and multivariate/univariate statistical analysis. Results: The results showed that T3 had the best therapeutic effect, and T1, T2 and T4 with no obvious therapeutic effect, demonstrating that the effective components of hawthorn should be compounds of medium polarity. T3 achieved good therapeutic effects due to the gastrointestinal motility promotion activity, and by rectifying the disturbed amino acid metabolism in gastrointestinal motility disorder model. Conclusion: This integrated metabolomics approach proved the validity of the therapeutic effect of extract from different polar parts of hawthorn on gastrointestinal motility disorder, providing new insights into the underlying mechanisms, and demonstrating the feasibility of metabolomics to evaluate efficacy of herbal drug, which is often difficult by traditional means.

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Mitochondrial Neuronal Gastrointestinal Encephalopathy (MNGIE): Differenzial diagnosis of gastrointestinal motility disorder, leucencephalopathy, peripheral neuropathy without mental retardation

Neuropediatrics ◽

10.1055/s-0031-1274102 ◽

2011 ◽

Vol 42 (S 01) ◽

Author(s):

S Illsinger ◽

C Schubert ◽

E Bültmann ◽

HJ Christen ◽

B Czermin ◽

...

Keyword(s):

Mental Retardation ◽

Peripheral Neuropathy ◽

Gastrointestinal Motility ◽

Motility Disorder ◽

Gastrointestinal Motility Disorder

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Melatonin Attenuates Noise Stress-induced Gastrointestinal Motility Disorder and Gastric Stress Ulcer: Role of Gastrointestinal Hormones and Oxidative Stress in Rats

Journal of Neurogastroenterology and Motility ◽

10.5056/jnm14119 ◽

2015 ◽

Vol 21 (2) ◽

pp. 189-199 ◽

Cited By ~ 15

Author(s):

Lei Zhang ◽

Ji T Gong ◽

Hu Q Zhang ◽

Quan H Song ◽

Guang H Xu ◽

...

Keyword(s):

Oxidative Stress ◽

Gastrointestinal Motility ◽

Stress Ulcer ◽

Gastrointestinal Hormones ◽

Motility Disorder ◽

Noise Stress ◽

Gastrointestinal Motility Disorder ◽

And Oxidative Stress

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Gastrointestinal motility disorder assessment in systemic sclerosis

Rheumatology ◽

10.1093/rheumatology/kes429 ◽

2013 ◽

Vol 52 (6) ◽

pp. 1095-1100 ◽

Cited By ~ 52

Author(s):

E. Savarino ◽

F. Mei ◽

A. Parodi ◽

M. Ghio ◽

M. Furnari ◽

...

Keyword(s):

Systemic Sclerosis ◽

Gastrointestinal Motility ◽

Motility Disorder ◽

Gastrointestinal Motility Disorder

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The Key Ingredient Acacetin in Weishu Decoction Alleviates Gastrointestinal Motility Disorder Based on Network Pharmacology Analysis

Mediators of Inflammation ◽

10.1155/2021/5265444 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Xuan Guo ◽

Yin Xu ◽

Hua-liang Tan ◽

Xiao-juan Wang ◽

Lin Xiao

Keyword(s):

Signaling Pathway ◽

Gastrointestinal Motility ◽

Akt Signaling ◽

Motility Disorder ◽

Flavonoid Compound ◽

Network Pharmacology ◽

High Dose ◽

Akt Signaling Pathway ◽

Motility Disorders ◽

Gastrointestinal Motility Disorder

Background. Gastrointestinal motility disorder is a common gastrointestinal disease, which seriously affects life quality. Traditional Chinese medicine (TCM) has been widely used as an alternative therapy for gastrointestinal motility disorder. Acacetin is a natural flavonoid compound that has antioxidant and anti-inflammatory, antidepressant, and anticancer properties. However, the efficacy of Acacetin in the treatment of gastrointestinal motility disorders has not been studied. Our aim was to investigate the mechanism of Acacetin-alleviated gastrointestinal motility disorder and its efficacy based on network pharmacology. Methods. We performed network pharmacology to predict the active components, match Weishu decoction (WSD) targets in gastrointestinal motility disorders, and investigate its potential pharmacological mechanisms. We performed the GO and KEGG enrichment analysis. In vivo, we investigated the effects of Acacetin in the gastrointestinal motility disorder model. Results. Based on network pharmacological method, the key active ingredient of WSD was identified as Acacetin, and the enrichment signaling pathway was the PI3K-AKT signaling pathway. Acacetin and Mosapride accelerated gastric emptying time, reduced gastric remnant rate, and increased small intestinal propulsion rate. The levels of GAS and MTL were increased after using Acacetin. These results indicated that Acacetin could improve gastrointestinal motility disorders. Among them, high-dose Acacetin showed a better effect. Acacetin could regulate protein and lipid metabolism in mice with gastrointestinal motility disorder. Furthermore, Acacetin could modulate gastrointestinal inflammation and apoptosis. The detection of the PI3K-AKT signaling pathway-related proteins showed that Acacetin improved gastrointestinal motility disorder by inhibiting the activation of the PI3K-AKT signaling pathway. Conclusion. The key ingredient Acacetin in WSD could alleviate gastrointestinal motility disorder by inhibiting the activation of the PI3K-AKT signaling pathway based on network pharmacology analysis. The efficacy and safety of Acacetin treatment provide strong experimental support for the clinical treatment of gastrointestinal motility disorder.

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