scholarly journals Potential Application of Human β-Defensin 4 in Dental Pulp Repair

2020 ◽  
Vol 11 ◽  
Author(s):  
Yue Zhai ◽  
Xiaojing Yuan ◽  
Yuming Zhao ◽  
Lihong Ge ◽  
Yuanyuan Wang
Keyword(s):  
Dental Pulp ◽  
Potential Application ◽  
Pulp Repair

Silver-Doped Bioactive Glass/Chitosan Hydrogel with Potential Application in Dental Pulp Repair

2019 ◽  
Vol 5 (9) ◽  
pp. 4624-4633
Author(s):  
Ningxin Zhu ◽  
Xanthippi Chatzistavrou ◽  
Petros Papagerakis ◽  
Lihong Ge ◽  
Man Qin ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Dental Pulp ◽  
Potential Application ◽  
Chitosan Hydrogel ◽  
Pulp Repair

scholarly journals Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jie Chen ◽  
Huaxing Xu ◽  
Kun Xia ◽  
Shuhua Cheng ◽  
Qi Zhang
Keyword(s):  
Dental Pulp ◽  
Tissue Repair ◽  
Pulp Tissue ◽  
Tissue Repair And Regeneration ◽  
Injury Model ◽  
Tnf Α ◽  
Pulp Inflammation ◽  
Inflammation Resolution ◽  
Pulp Repair ◽  
Dentin Regeneration

Abstract Background Unresolved inflammation and tissue destruction are considered to underlie the failure of dental pulp repair. As key mediators of the injury response, dental pulp stem cells (DPSCs) play a critical role in pulp tissue repair and regeneration. Resolvin E1 (RvE1), a major dietary omega-3 polyunsaturated fatty-acid metabolite, is effective in resolving inflammation and activating wound healing. However, whether RvE1 facilitates injured pulp-tissue repair and regeneration through timely resolution of inflammation and rapid mobilization of DPSCs is unknown. Therefore, we established a pulp injury model and investigated the effects of RvE1 on DPSC-mediated inflammation resolution and injured pulp repair. Methods A pulp injury model was established using 8-week-old Sprague-Dawley rats. Animals were sacrificed on days 1, 3, 7, 14, 21, and 28 after pulp capping with a collagen sponge immersed in PBS with RvE1 or PBS. Hematoxylin-eosin and Masson’s trichrome staining, immunohistochemistry, and immunohistofluorescence were used to evaluate the prohealing properties of RvE1. hDPSCs were incubated with lipopolysaccharide (LPS) to induce an inflammatory response, and the expression of inflammatory factors after RvE1 application was measured. Effects of RvE1 on hDPSC proliferation, chemotaxis, and odontogenic differentiation were evaluated by CCK-8 assay, transwell assay, alkaline phosphatase (ALP) staining, alizarin red staining, and quantitative PCR, and possible signaling pathways were explored using western blotting. Results In vivo, RvE1 reduced the necrosis rate of damaged pulp and preserved more vital pulps, and promoted injured pulp repair and reparative dentin formation. Further, it enhanced dentin matrix protein 1 and dentin sialoprotein expression and accelerated pulp inflammation resolution by suppressing TNF-α and IL-1β expression. RvE1 enhanced the recruitment of CD146+ and CD105+ DPSCs to the damaged molar pulp mesenchyme. Isolated primary cells exhibited the mesenchymal stem cell immunophenotype and differentiation. RvE1 promoted hDPSC proliferation and chemotaxis. RvE1 significantly attenuated pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) release and enhanced ALP activity, nodule mineralization, and especially, expression of the odontogenesis-related genes DMP1, DSPP, and BSP in LPS-stimulated DPSCs. RvE1 regulated AKT, ERK, and rS6 phosphorylation in LPS-stimulated DPSCs. Conclusions RvE1 promotes pulp inflammation resolution and dentin regeneration and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. This response is, at least partially, dependent on AKT, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 has promising application potential in regenerative endodontics.

In vitro and in vivo evaluation of a nanoparticulate bioceramic paste for dental pulp repair

2014 ◽  
Vol 10 (12) ◽  
pp. 5156-5168 ◽  
Author(s):  
Lingxin Zhu ◽  
Jingwen Yang ◽  
Jie Zhang ◽  
Dongqi Lei ◽  
Lan Xiao ◽  
...  
Keyword(s):  
Dental Pulp ◽  
In Vivo Evaluation ◽  
Pulp Repair

Effects of Alginate Hydrogels and TGF-β1 on Human Dental Pulp Repair In Vitro

2002 ◽  
Vol 43 (2) ◽  
pp. 387-390 ◽  
Author(s):  
K. Dobie ◽  
G. Smith ◽  
A. J. Sloan ◽  
A. J. Smith
Keyword(s):  
Dental Pulp ◽  
Alginate Hydrogels ◽  
Human Dental Pulp ◽  
Pulp Repair ◽  
Tgf Β1

scholarly journals The Regenerative Potential of bFGF in Dental Pulp Repair and Regeneration

2021 ◽  
Vol 12 ◽  
Author(s):  
Keyue Liu ◽  
Sijing Yu ◽  
Ling Ye ◽  
Bo Gao
Keyword(s):  
Growth Factors ◽  
Dental Pulp ◽  
Spinal Cord Injuries ◽  
Healing Process ◽  
Fibroblast Growth Factors ◽  
Wound Healing Process ◽  
Fibroblast Growth ◽  
Pulp Tissue ◽  
Tissue Repair And Regeneration ◽  
Pulp Repair

Regenerative endodontic therapy intends to induce the host’s natural wound-healing process, which can restore the vitality, immunity, and sensitivity of the inflammatory or necrotic pulp tissue destroyed by infection or trauma. Myriads of growth factors are critical in the processes of pulp repair and regeneration. Among the key regulatory factors are the fibroblast growth factors, which have turned out to be the master regulators of both organogenesis and tissue homeostasis. Fibroblast growth factors, a family composed of 22 polypeptides, have been used in tissue repair and regeneration settings, in conditions as diverse as burns, ulcers, bone-related diseases, and spinal cord injuries. Meanwhile, in dentistry, the basic fibroblast growth factor is the most frequently investigated. Thereby, the aim of this review is 2-fold: 1) foremost, to explore the underlying mechanisms of the bFGF in dental pulp repair and regeneration and 2) in addition, to shed light on the potential therapeutic strategies of the bFGF in dental pulp–related clinical applications.

Promotion of the Differentiation of Dental Pulp Stem Cells into Oligodendrocytes by Knockdown of Heat-Shock Protein 27

2022 ◽  
Author(s):  
Shu-Lin Guo ◽  
Chih-Hui Chin ◽  
Chi-Jung Huang ◽  
Chih-Cheng Chien ◽  
Yih-Jing Lee
Keyword(s):  
Stem Cells ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Potential Application ◽  
Neural Differentiation ◽  
Heat Shock Protein 27 ◽  
Dental Pulp Stem Cells ◽  
Immunofluorescent Staining

Stem cell-based therapy has been evaluated in many different clinical trials for various diseases. This capability was applied in various neurodegenerative diseases, such as Alzheimer’s disease, which is characterized by synaptic damage accompanied by neuronal loss. Dental pulp stem cells (DPSCs) are mesenchymal stem cells from the oral cavity and have been studied with potential application for regeneration of different tissues. Heat shock protein 27 (HSP27) is known to regulate neurogenesis in the process of neural differentiation of placenta-multipotent stem cells. Here, we hypothesize that HSP27 expression is also critical in neural differentiation of DPSCs. An evaluation of the possible role of HSP27 in differentiation of DPSCs was per-formed by gene knockdown and neural immunofluorescent staining. We found that HSP27 has a role in the differentiation of DPSCs and that knockdown of HSP27 in DPSCs renders cells to oligodendrocyte progenitors. In other words, shHSP27-DPSCs showed NG2-positive immunoreactivity and gave rise to oligodendrocytes or type-2 astrocytes. This neural differentiation of DPSCs may have clinical significance for treatment of patients with neurodegenerative diseases. In conclusion, our data provide an example of oligodendrocyte differentiation of a DPSCs model that may have potential application in human regenerative medicine.

Evaluation of dental pulp repair using low level laser therapy (688 nm and 785 nm) morphologic study in capuchin monkeys

2009 ◽  
Vol 6 (2) ◽  
pp. 149-158 ◽  
Author(s):  
H. Pretel ◽  
J.A. Oliveira ◽  
R.F.Z. Lizarelli ◽  
L.T.O. Ramalho
Keyword(s):  
Laser Therapy ◽  
Dental Pulp ◽  
Low Level Laser Therapy ◽  
Capuchin Monkeys ◽  
Morphologic Study ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Pulp Repair

Detection of bone marrow-derived fibrocytes in human dental pulp repair

2018 ◽  
Vol 51 (11) ◽  
pp. 1187-1195 ◽  
Author(s):  
N. Yoshiba ◽  
N. Edanami ◽  
A. Tohma ◽  
R. Takeuchi ◽  
N. Ohkura ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dental Pulp ◽  
Human Dental Pulp ◽  
Pulp Repair

An in vitro Model of Human Dental Pulp Repair

1996 ◽  
Vol 75 (12) ◽  
pp. 1971-1978 ◽  
Author(s):  
H. Magloire ◽  
A. Joffre ◽  
F. Bleicher
Keyword(s):  
Dental Pulp ◽  
In Vitro Model ◽  
Human Dental Pulp ◽  
Vitro Model ◽  
Pulp Repair
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