scholarly journals Cloning and Expression of the Neuropeptide F and Neuropeptide F Receptor Genes and Their Regulation of Food Intake in the Chinese White Pine Beetle Dendroctonus armandi

2021 ◽  
Vol 12 ◽  
Author(s):  
Bin Liu ◽  
Danyang Fu ◽  
Haiming Gao ◽  
Hang Ning ◽  
Yaya Sun ◽  
...  
Keyword(s):  
Food Intake ◽  
Developmental Stages ◽  
Amino Acid Sequences ◽  
Cloning And Expression ◽  
White Pine ◽  
Positive Role ◽  
Chinese White ◽  
Pine Beetle ◽  
Neuropeptide F ◽  
G Protein Coupled

Neuropeptide F (NPF) is an important signaling molecule that acts as a neuromodulator to regulate a diversity of physiological and behavioral processes from vertebrates to invertebrates by interaction with NPF receptors, which are G protein-coupled receptors (GPCR). However, nothing is known about NPF in Chinese white pine beetle, Dendroctonus armandi, a destructive pest of natural and coniferous forests in the middle Qinling Mountains of China. We have cloned and characterized cDNAs encoding one NPF precursor and two NPF receptors in D. armandi and made bioinformatics predictions according to the deduced amino acid sequences. They were highly similar to that of Dendroctonus ponderosa. The transcription levels of these genes were different between larvae and adults of sexes, and there were significant differences among the different developmental stages and tissues and between beetles under starvation and following re-feeding states. Additionally, downregulation of NPF and NPFR by injecting dsRNA into beetles reduced their food intake, caused increases of mortality and decreases of body weight, and also resulted in a decrease of glycogen and free fatty acid and an increase of trehalose. These results indicate that the NPF signaling pathway plays a significant positive role in the regulation of food intake and provides a potential target for the sustainable management of this pest.

scholarly journals Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in Dendroctonus armandi

Insects ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 844
Author(s):  
Bin Liu ◽  
Danyang Fu ◽  
Hang Ning ◽  
Ming Tang ◽  
Hui Chen
Keyword(s):  
Food Intake ◽  
Developmental Stages ◽  
Control Strategies ◽  
Amino Acid Sequences ◽  
Body Parts ◽  
Expression Levels ◽  
Reverse Transcription Pcr ◽  
Evolutionarily Conserved ◽  
Neuropeptide F ◽  
High Degree

The short neuropeptide F (sNPF) is an essential signaling molecule that is evolutionarily conserved and involved in a broad range of physiological functions in the invertebrates, by interacting with sNPF receptors, which belong to G protein-coupled receptors (GPCR). However, the function of sNPF in regulating the food intake of Dendroctonus armandi has been unclear. In this study, we cloned and characterized cDNAs encoding sNPF and sNPF receptor in the D. armandi and made bioinformatics predictions on the deduced amino acid sequences. They had a high degree of similarity to that of Dendroctonus ponderosa. Quantitative real-time reverse transcription PCR (qRT-PCR) revealed that the transcript levels of both sNPF and sNPFR varied across developmental stages and body parts. In addition, the sNPF and sNPFR expression levels were upregulated in starved beetles, and the expression levels recovered after re-feeding. Furthermore, RNAi knockdown by the injection of sNPF and sNPFR dsRNA into beetles significantly increased mortality and reduced their food intake and body weight, and also caused decrease of glycogen and free fatty acid and increase of trehalose. These results indicate that sNPF signaling pathway plays an important role in the regulation of food intake and provides a potential molecular target for the eco-friendly control strategies of this pest.

Isolation of CarE genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defense

2018 ◽  
Vol 75 (4) ◽  
pp. 986-997 ◽  
Author(s):  
Lulu Dai ◽  
Haiming Gao ◽  
Jiaqi Ye ◽  
Danyang Fu ◽  
Yaya Sun ◽  
...  
Keyword(s):  
Chemical Defense ◽  
White Pine ◽  
Chinese White ◽  
Pine Beetle

Survival physiology and sex ratio of the Chinese white pine beetle Dendroctonus armandi (Coleoptera: Scolytinae) during host colonization and overwintering

2019 ◽  
Vol 110 (1) ◽  
pp. 115-122
Author(s):  
L. Dai ◽  
J. Zheng ◽  
Y. Wang ◽  
Y. Sun ◽  
H. Chen
Keyword(s):  
Sex Ratio ◽  
Host Plant ◽  
Body Mass ◽  
The Body ◽  
Mass Energy ◽  
White Pine ◽  
Host Colonization ◽  
Chinese White ◽  
Pine Beetle ◽  
Energy Stores

AbstractThe Chinese white pine beetle Dendroctonus armandi (Coleoptera: Scolytinae) typically displays bivoltinism at altitudes below 1700 m in the Qinling Mountains, China. The periods of host colonization and larval overwintering are two important phases in the life cycle of bark beetles, as it is during these periods that they have to contend with host plant defences and periods of intense cold, respectively. Although during different seasons, the females and males of Chinese white pine beetles show varying tolerances to host plant terpenoids, the sex ratio and survival physiology condition of the two beetle generations are unknown. We investigated the sex ratio of individuals, and also examined the body mass, energy stores, and detoxication enzymes of males and females in each of the two generations in order to determine the overall population stability of each generation. We identified a female-biased sex ratio among adults in both generations. Furthermore, patterns of body mass, energy stores, and detoxication enzymes were found to differ between the two sexes and two seasons. Compared with the males, the females have a larger body mass and higher amounts of stored lipids, which are assumed to be adaptations designed to overcome host resistance and facilitate subsequent oviposition.

scholarly journals Phylogeny of Leptographium Qinlingensis Cytochrome P450 Genes and Their Expression When Grown on Different Media or Treated With Terpenoids

2021 ◽  
Author(s):  
Lulu Dai ◽  
Jie Zheng ◽  
Jiaqi Ye ◽  
Hui Chen
Keyword(s):  
Cytochrome P450 ◽  
Sole Carbon Source ◽  
Common Ancestor ◽  
Expression Profiles ◽  
White Pine ◽  
Host Trees ◽  
Chinese White ◽  
Cytochrome P450 Genes ◽  
Pine Beetle ◽  
Fungal Associate

Abstract Leptographium qinlingensis is a fungal associate of the Chinese white pine beetle (Dendroctonus armandi) and a pathogen of the Chinese white pine (Pinus armandi) that must overcome the terpenoid oleoresin defences of host trees. We identified and phylogenetically analysed the cytochrome P450 (CYP) genes in the transcriptome of L. qinlingensis. Through analyses of the growth rates on different nutritional media and inhibition by terpenoids, the expression profiles of six CYPs in the mycelium of L. qinlingensis grown on different media or treated with terpenoids were determined. The CYP evolution predicted that most of the CYPs occurred in a putative common ancestor shared between L. qinlingensis and G. clavigera. This fungus is symbiotic with D. armandi and has more similarity with G. clavigera, which can retrieve nutrition from pine wood and utilize monoterpenes as the sole carbon source. Some CYP genes might be involved in the metabolism of fatty acids and detoxification of terpenes and phenolics, as observed in other blue-stained fungi, which also indicates the pathogenic properties of L. qinlingensis in Chinese white pine.

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