scholarly journals Automated Classification of Whole Body Plethysmography Waveforms to Quantify Breathing Patterns

2021 ◽  
Vol 12 ◽  
Author(s):  
Michael D. Sunshine ◽  
David D. Fuller
Keyword(s):  
Cluster Analysis ◽  
Ampa Receptors ◽  
Rapid Assessment ◽  
Whole Body ◽  
Automated Classification ◽  
Body Plethysmography ◽  
Sprague Dawley ◽  
Breathing Patterns ◽  
Clustering Method ◽  
Whole Body Plethysmography

Whole body plethysmography (WBP) monitors respiratory rate and depth but conventional analysis fails to capture the diversity of waveforms. Our first purpose was to develop a waveform cluster analysis method for quantifying dynamic changes in respiratory waveforms. WBP data, from adult Sprague-Dawley rats, were sorted into time domains and principle component analysis was used for hierarchical clustering. The clustering method effectively sorted waveforms into categories including sniffing, tidal breaths of varying duration, and augmented breaths (sighs). We next used this clustering method to quantify breathing after opioid (fentanyl) overdose and treatment with ampakine CX1942, an allosteric modulator of AMPA receptors. Fentanyl caused the expected decrease in breathing, but our cluster analysis revealed changes in the temporal appearance of inspiratory efforts. Ampakine CX1942 treatment shifted respiratory waveforms toward baseline values. We conclude that this method allows for rapid assessment of breathing patterns across extended data recordings. Expanding analyses to include larger portions of recorded WBP data may provide insight on how breathing is affected by disease or therapy.

scholarly journals Measuring Breathing Patterns in Mice Using Whole-body Plethysmography

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (17) ◽  
Author(s):  
Patricia Prada-Dacasa ◽  
Andrea Urpi ◽  
Laura Sánchez-Benito ◽  
Patrizia Bianchi ◽  
Albert Quintana
Keyword(s):  
Whole Body ◽  
Body Plethysmography ◽  
Breathing Patterns ◽  
Whole Body Plethysmography

scholarly journals Novel whole body plethysmography system for the continuous characterization of sleep and breathing in a mouse

2012 ◽  
Vol 112 (4) ◽  
pp. 671-680 ◽  
Author(s):  
A. B. Hernandez ◽  
J. P. Kirkness ◽  
P. L. Smith ◽  
H. Schneider ◽  
M. Polotsky ◽  
...  
Keyword(s):  
Tidal Volume ◽  
Gold Standard ◽  
Inbred Mouse ◽  
Respiratory Movement ◽  
Whole Body ◽  
Mouse Strains ◽  
Obese Mouse ◽  
Body Plethysmography ◽  
Breathing Patterns ◽  
Whole Body Plethysmography

Sleep is associated with marked alterations in ventilatory control that lead to perturbations in respiratory timing, breathing pattern, ventilation, pharyngeal collapsibility, and sleep-related breathing disorders (SRBD). Mouse models offer powerful insight into the pathogenesis of SRBD; however, methods for obtaining the full complement of continuous, high-fidelity respiratory, electroencephalographic (EEG), and electromyographic (EMG) signals in unrestrained mice during sleep and wake have not been developed. We adapted whole body plethysmography to record EEG, EMG, and respiratory signals continuously in unrestrained, unanesthetized mice. Whole body plethysmography tidal volume and airflow signals and a novel noninvasive surrogate for respiratory effort (respiratory movement signal) were validated against simultaneously measured gold standard signals. Compared with the gold standard, we validated 1) tidal volume (correlation, R2 = 0.87, P < 0.001; and agreement within 1%, P < 0.001); 2) inspiratory airflow (correlation, R2 = 0.92, P < 0.001; agreement within 4%, P < 0.001); 3) expiratory airflow (correlation, R2 = 0.83, P < 0.001); and 4) respiratory movement signal (correlation, R2 = 0.79–0.84, P < 0.001). The expiratory airflow signal, however, demonstrated a decrease in amplitude compared with the gold standard. Integrating respiratory and EEG/EMG signals, we fully characterized sleep and breathing patterns in conscious, unrestrained mice and demonstrated inspiratory flow limitation in a New Zealand Obese mouse. Our approach will facilitate studies of SRBD mechanisms in inbred mouse strains and offer a powerful platform to investigate the effects of environmental and pharmacological exposures on breathing disturbances during sleep and wakefulness.

scholarly journals First case reported of bronchoconstriction in feline aelurostrongylosis by using barometric whole-body plethysmography

2018 ◽  
Vol 66 (2) ◽  
pp. 101
Author(s):  
L. GARCÍA-GUASCH ◽  
J. MANUBENS ◽  
M. LAPORTA ◽  
E. CARRETÓN ◽  
J. A. MONTOYA-ALONSO
Keyword(s):  
Respiratory Disease ◽  
Clinical Signs ◽  
Bronchial Tree ◽  
Whole Body ◽  
Body Plethysmography ◽  
Breathing Patterns ◽  
Non Invasive ◽  
First Case ◽  
Pulmonary Inflammatory Response ◽  
Whole Body Plethysmography

Symptomatic cats infected by Aelurostrongylus abstrusus show non-specific and respiratory clinical signs, often misdiagnosed as other diseases more prevalent among feline population, such as allergic respiratory disease or heartworm associated respiratory disease (HARD). Clinical signs are due to the pulmonary inflammatory response caused by the eggs shed by the adult females and the migration of the first-stage larvae up the bronchial tree. Barometric whole-body plethysmography (BWBP) is a non-invasive pulmonary function test that allows a dynamic study of breathing patterns by placing the patient within an unrestrained Plexiglas chamber. This is the first report that determines the degree of bronchoconstriction caused by A. abstrusus infection in a cat by using BWBP, showing an increase of baseline measurements of bronchoconstriction indexes (Enhanced pause and Pause) in response to severe bronchial reactivity, a consequence of the airway inflammation caused by the presence of A. abstrusus.

Whole-body plethysmography

2005 ◽  
pp. 15-43 ◽  
Author(s):  
M.D. Goldman ◽  
H.J. Smith ◽  
W.T. Ulmer
Keyword(s):  
Whole Body ◽  
Body Plethysmography ◽  
Whole Body Plethysmography

Maturation of baseline breathing and of hypercapnic and hypoxic ventilatory responses in newborn mice

2001 ◽  
Vol 281 (5) ◽  
pp. R1746-R1753 ◽  
Author(s):  
Sylvain Renolleau ◽  
Stéphane Dauger ◽  
Fanny Autret ◽  
Guy Vardon ◽  
Claude Gaultier ◽  
...  
Keyword(s):  
Cesarean Section ◽  
Tidal Volume ◽  
Mammalian Species ◽  
Whole Body ◽  
Body Plethysmography ◽  
Genetic Studies ◽  
Newborn Mice ◽  
Ventilatory Responses ◽  
Neuronal Mechanisms ◽  
Whole Body Plethysmography

Breathing during the first postnatal hours has not been examined in mice, the preferred mammalian species for genetic studies. We used whole body plethysmography to measure ventilation (V˙e), breath duration (TTOT), and tidal volume (Vt) in mice delivered vaginally (VD) or by cesarean section (CS). In experiment 1, 101 VD and 100 CS pups aged 1, 6, 12, 24, or 48 h were exposed to 8% CO2 or 10% O2for 90 s. In experiment 2, 31 VD pups aged 1, 12, or 24 h were exposed to 10% O2 for 5 min. Baseline breathing maturation was delayed in CS pups, but V˙eresponses to hypercapnia and hypoxia were not significantly different between VD and CS pups [at postnatal age of 1 h (H1): 48 ± 44 and 18 ± 32%, respectively, in VD and CS pups combined]. TheV˙e increase induced by hypoxia was greater at H12 (46 ± 27%) because of TTOT response maturation. At all ages, hypoxic decline was ascribable mainly to a Vtdecrease, and posthypoxic decline was ascribable to a TTOTincrease with apneas, suggesting different underlying neuronal mechanisms.

scholarly journals Early and late allergic reaction in the nose assessed by whole body plethysmography

1996 ◽  
Vol 9 (8) ◽  
pp. 1701-1706 ◽  
Author(s):  
M.S. de Bruin-Weller ◽  
F.R. Weller ◽  
A. Scholte ◽  
L.H.M. Rijssenbeek ◽  
S. van der Baan ◽  
...  
Keyword(s):  
Allergic Reaction ◽  
Whole Body ◽  
Body Plethysmography ◽  
Whole Body Plethysmography
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