scholarly journals TRP Channel Agonists Activate Different Afferent Neuromodulatory Mechanisms in Guinea Pig Urinary Bladder

2021 ◽  
Vol 12 ◽  
Author(s):  
Stephanie L. Daugherty ◽  
Jonathan M. Beckel ◽  
Kyoungeun A. Kim ◽  
Bruce A. Freeman ◽  
Jiaxin Liu ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Guinea Pig ◽  
Trp Channels ◽  
Allyl Isothiocyanate ◽  
Inhibitory Effect ◽  
Bladder Function ◽  
Trpc Channels ◽  
Afferent Nerves ◽  
Bladder Activity ◽  
Trpv1 Antagonist

Activation of TRP channels expressed in urinary bladder afferent nerves and urothelium releases neurotransmitters that influence bladder function. Experiments were undertaken to examine the mechanisms underlying effects of TRPA1 (allyl isothiocyanate, AITC), TRPV1 (capsaicin, CAPS), and TRPC (oleoyl-2-acetyl-sn-glycerol, OAG) agonists on guinea pig bladder activity. Effects of these agonists were compared with effects of nitro-oleic acid (OA-NO2), an electrophilic nitro-fatty acid, known to activate TRPV1, TRPA1 or TRPC channels in sensory neurons. AITC (100 μM) increased (231%) area of spontaneous bladder contractions (SBCs) an effect reduced by a TRPA1 antagonist (HC3-03001, HC3, 10 μM) and reversed to inhibition by indomethacin (INDO, 500 nM) a cyclooxygenase inhibitor. The post-INDO inhibitory effect of AITC was mimicked (39% depression) by calcitonin gene-related peptide (CGRP, 100 nM) and blocked by a CGRP antagonist (BIBN, 25 μM). CAPS (1 μM) suppressed SBCs by 30% in 81% of strips, an effect blocked by a TRPV1 antagonist (diarylpiperazine, 1 μM) or BIBN. SBCs were suppressed by OA-NO2 (30 μM, 21% in 77% of strips) or by OAG (50 μM, 30%) an effect blocked by BIBN. OA-NO2 effects were not altered by HC3 or diarylpiperazine. OA-NO2 also induced excitation in 23% of bladder strips. These observations raise the possibility that guinea pig bladder is innervated by at least two types of afferent nerves: [1] Type A express TRPA1 receptors that induce the release of prostaglandins and excite the detrusor, [2] Type B express TRPV1, TRPA1 and TRPC receptors and release CGRP that inhibits the detrusor.

Guinea pig as an animal model for the study of urinary bladder function in the normal and obstructed state

1994 ◽  
Vol 13 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Jacek L. Mostwin ◽  
Omer M. A. Karim ◽  
Gommert Van Koeveringe ◽  
Narihito Seki
Keyword(s):  
Animal Model ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Bladder Function

Regulation of [3H]GABA release from strips of guinea pig urinary bladder

1988 ◽  
Vol 255 (6) ◽  
pp. R888-R893
Author(s):  
J. Shirakawa ◽  
K. Taniyama ◽  
S. Iwai ◽  
C. Tanaka
Keyword(s):  
Urinary Bladder ◽  
Guinea Pig ◽  
Adrenergic Receptors ◽  
Gaba Release ◽  
Inhibitory Effect ◽  
Atropine Sulfate ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
High K ◽  
M1 Muscarinic

The presence of receptors that regulate the release of gamma-aminobutyric acid (GABA) was studied in strips of the guinea pig urinary bladder. GABA (10(-8)-10(-5) M) and muscimol (10(-8)-10(-5) M), but not baclofen (10(-5) M), reduced the Ca2+-dependent, tetrodotoxin-resistant release of [3H]GABA evoked by high K+ from the urinary bladder strips preloaded with [3H]GABA. The inhibitory effect of muscimol was antagonized by bicuculline and potentiated by diazepam, clonazepam, and pentobarbital sodium. The potentiating effect of clonazepam was antagonized by Ro 15-1788. Acetylcholine (ACh) inhibited the high K+-evoked release of [3H]GABA. The inhibitory effect of ACh was antagonized by atropine sulfate and pirenzepine but not by hexamethonium. Norepinephrine (NE) inhibited the evoked release of [3H]GABA. The inhibitory effect of NE was mimicked by clonidine, but not by phenylephrine, and was antagonized by yohimbine but not by prazosin. These results provide evidence that the release of GABA from strips of guinea pig urinary bladder is regulated via the bicuculline-sensitive GABAA receptor, M1-muscarinic, and alpha 2-adrenergic receptors.

scholarly journals Peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) activates capsaicin-sensitive primary afferent nerves in guinea-pig atria and urinary bladder

1991 ◽  
Vol 102 (3) ◽  
pp. 730-734 ◽  
Author(s):  
Sandro Giuliani ◽  
Paolo Santicioli ◽  
Manuela Tramontana ◽  
Pierangelo Geppetti ◽  
Carlo Alberto Maggi
Keyword(s):  
Urinary Bladder ◽  
Guinea Pig ◽  
Primary Afferent ◽  
Afferent Nerves ◽  
Primary Afferent Nerves ◽  
Guinea Pig Atria

scholarly journals Post-Stimulation Inhibitory Effect on Reflex Bladder Activity Induced by Activation of Somatic Afferent Nerves in the Foot

2012 ◽  
Vol 187 (1) ◽  
pp. 338-343 ◽  
Author(s):  
Guoqing Chen ◽  
Jeffrey A. Larson ◽  
P. Dafe Ogagan ◽  
Bing Shen ◽  
Jicheng Wang ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Afferent Nerves ◽  
Bladder Activity

scholarly journals Unique properties of muscularis mucosae smooth muscle in guinea pig urinary bladder

2011 ◽  
Vol 301 (2) ◽  
pp. R351-R362 ◽  
Author(s):  
Thomas J. Heppner ◽  
Jeffrey J. Layne ◽  
Jessica M. Pearson ◽  
Hagop Sarkissian ◽  
Mark T. Nelson
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Purinergic Receptor ◽  
Bk Channels ◽  
Bk Channel ◽  
Cholinergic Receptors ◽  
Bladder Function ◽  
Muscularis Mucosae ◽  
Maximal Force

The muscularis mucosae, a type of smooth muscle located between the urothelium and the urinary bladder detrusor, has been described, although its properties and role in bladder function have not been characterized. Here, using mucosal tissue strips isolated from guinea pig urinary bladders, we identified spontaneous phasic contractions (SPCs) that appear to originate in the muscularis mucosae. This smooth muscle layer exhibited Ca2+ waves and flashes, but localized Ca2+ events (Ca2+ sparks, purinergic receptor-mediated transients) were not detected. Ca2+ flashes, often in bursts, occurred with a frequency (∼5.7/min) similar to that of SPCs (∼4/min), suggesting that SPCs are triggered by bursts of Ca2+ flashes. The force generated by a single mucosal SPC represented the maximal force of the strip, whereas a single detrusor SPC was ∼3% of maximal force of the detrusor strip. Electrical field stimulation (0.5–50 Hz) evoked force transients in isolated detrusor and mucosal strips. Inhibition of cholinergic receptors significantly decreased force in detrusor and mucosal strips (at higher frequencies). Concurrent inhibition of purinergic and cholinergic receptors nearly abolished evoked responses in detrusor and mucosae. Mucosal SPCs were unaffected by blocking small-conductance Ca2+-activated K+ (SK) channels with apamin and were unchanged by blocking large-conductance Ca2+-activated K+ (BK) channels with iberiotoxin (IbTX), indicating that SK and BK channels play a much smaller role in regulating muscularis mucosae SPCs than they do in regulating detrusor SPCs. Consistent with this, BK channel current density in myocytes from muscularis mucosae was ∼20% of that in detrusor myocytes. These findings indicate that the muscularis mucosae in guinea pig represents a second smooth muscle compartment that is physiologically and pharmacologically distinct from the detrusor and may contribute to the overall contractile properties of the urinary bladder.

Effects of capsaicin on micturition and associated reflexes in rats

1993 ◽  
Vol 265 (1) ◽  
pp. R132-R138 ◽  
Author(s):  
C. L. Cheng ◽  
C. P. Ma ◽  
W. C. de Groat
Keyword(s):  
Urinary Bladder ◽  
Large Dose ◽  
Urethral Catheter ◽  
Female Rats ◽  
Bladder Function ◽  
Intravesical Pressure ◽  
Pressor Responses ◽  
Bladder Activity ◽  
Micturition Volume ◽  
Stimulation Of

The effect of capsaicin on micturition and associated reflexes was studied in urethan-anesthetized female rats. Capsaicin or vehicle solution were administered 4 days before the experiment in a dose of 125 mg/kg sc or during the experiment in a dose of 50-100 mg/kg sc. Activity of the urinary bladder was recorded by measuring intravesical pressure via a urethral catheter while slowly filling (0.052 ml/min) the bladder or when the bladder was distended beyond the micturition threshold and maintained at a constant volume. Pretreatment with capsaicin did not significantly change various parameters of urinary bladder function including micturition volume threshold or the amplitude, duration, and interval between reflex bladder contractions. However, capsaicin pretreatment significantly reduced (80% decrease) the arterial pressor responses accompanying reflex bladder contractions and reduced by approximately one-half the percentage of animals in which bladder activity was inhibited by stimulation of the uterine cervix. A large dose of capsaicin (50 mg/kg sc) elicited an acute block of bladder activity that persisted for 8-15 h. This effect is attributable to an action on myelinated afferent or efferent components of the micturition reflex pathway. It is concluded that capsaicin-sensitive afferents are not essential for the performance of micturition in the rat. However, these afferents are involved in cervicovesical reflex mechanisms that modulate bladder activity and in vascular reflexes triggered by isometric bladder contractions.

Effects of Sensitization on Female Guinea Pig Urinary Bladder Function: In Vivo and in Vitro Studies

1991 ◽  
Vol 146 (2 Part 1) ◽  
pp. 454-457 ◽  
Author(s):  
Young Soo Kim ◽  
Penelope A. Longhurst ◽  
Alan J. Wein ◽  
Robert M. Levin
Keyword(s):  
Urinary Bladder ◽  
Guinea Pig ◽  
In Vitro Studies ◽  
Bladder Function

Effect of Low Threshold Versus High Threshold Genitalia Stimuli on the Cystometry Parameters in Male Rats

2021 ◽  
Author(s):  
Ezidin G. Kaddumi ◽  
Ali Al Khader ◽  
Sakher Tahaineh
Keyword(s):  
Urinary Bladder ◽  
Inhibitory Effect ◽  
Male Rats ◽  
Bladder Function ◽  
High Threshold ◽  
Pressure Amplitude ◽  
Micturition Reflex ◽  
Pathological Conditions ◽  
Significant Inhibitory Effect ◽  
Low Threshold

The cross talk between external genitalia and urinary bladder could be utilized as part of management to certain pathological conditions affecting urinary bladder. Since urinary bladder function is also affected by pathologies of other organs (e.g., colon and esophagus), the effect of genitalia stimuli on parameters of bladder function in normal or under different pathological conditions needs to be characterized. Cystometry recordings in male rats were used to examine the effect of low threshold (LT) and high threshold (HT) stimulation of the scrotum and penis on urinary bladder function. These effects were studied in intact, colon irritation (CI), and esophagus irritation (EI) groups. Although, HT penile stimulation had a significant inhibitory effect on micturition reflex in all groups, CI hypersensitized the penile-bladder inhibitory reflex. In addition, LT penile stimulation had a significant inhibitory effect on micturition, which was significant in CI group, only. On the other hand, HT penile stimulation, in CI group, significantly increased the timing parameters of cystometry. While, LT and HT penile stimuli, in EI group, had a significantly increasing effect on all pressure parameters of cystometry. The scrotal stimuli had minimal effect on bladder function in all groups except for HT scrotal stimulation in the CI group, where it had a significant inhibitory effect on micturition reflex and significantly increased the maximum pressure and pressure amplitude of micturition cycles. These results show that CI and EI exacerbate the effects of genitalia stimuli, especially penile stimuli, on urinary bladder function.

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