Effects of capsaicin on micturition and associated reflexes in rats

1993 ◽  
Vol 265 (1) ◽  
pp. R132-R138 ◽  
Author(s):  
C. L. Cheng ◽  
C. P. Ma ◽  
W. C. de Groat
Keyword(s):  
Urinary Bladder ◽  
Large Dose ◽  
Urethral Catheter ◽  
Female Rats ◽  
Bladder Function ◽  
Intravesical Pressure ◽  
Pressor Responses ◽  
Bladder Activity ◽  
Micturition Volume ◽  
Stimulation Of

The effect of capsaicin on micturition and associated reflexes was studied in urethan-anesthetized female rats. Capsaicin or vehicle solution were administered 4 days before the experiment in a dose of 125 mg/kg sc or during the experiment in a dose of 50-100 mg/kg sc. Activity of the urinary bladder was recorded by measuring intravesical pressure via a urethral catheter while slowly filling (0.052 ml/min) the bladder or when the bladder was distended beyond the micturition threshold and maintained at a constant volume. Pretreatment with capsaicin did not significantly change various parameters of urinary bladder function including micturition volume threshold or the amplitude, duration, and interval between reflex bladder contractions. However, capsaicin pretreatment significantly reduced (80% decrease) the arterial pressor responses accompanying reflex bladder contractions and reduced by approximately one-half the percentage of animals in which bladder activity was inhibited by stimulation of the uterine cervix. A large dose of capsaicin (50 mg/kg sc) elicited an acute block of bladder activity that persisted for 8-15 h. This effect is attributable to an action on myelinated afferent or efferent components of the micturition reflex pathway. It is concluded that capsaicin-sensitive afferents are not essential for the performance of micturition in the rat. However, these afferents are involved in cervicovesical reflex mechanisms that modulate bladder activity and in vascular reflexes triggered by isometric bladder contractions.

scholarly journals Effect of distal esophageal irritation on the changes of cystometry parameters to esophagus and colon distentions in rats

2019 ◽  
Vol 97 (8) ◽  
pp. 766-772
Author(s):  
Ezidin G. Kaddumi
Keyword(s):  
Electrical Stimulation ◽  
Urinary Bladder ◽  
Vagus Nerve ◽  
Storage Time ◽  
Distal Colon ◽  
Bladder Function ◽  
Maximum Pressure ◽  
Pressure Amplitude ◽  
Chemical Irritation ◽  
Stimulation Of

The coexistence of different visceral pathologies in patients suffering from irritable bowel syndrome, interstitial cystitis, and other pathologies, necessitates the study of these pathologies under complicated conditions. In the present study, cystometry recordings were used to investigate the effect of distal esophageal chemical irritation on the urinary bladder interaction with distal colon distention, distal esophageal distention, and electrical stimulation of abdominal branches of vagus nerve. Distal esophageal chemical irritation significantly decreased the intercontraction time via decreasing the voiding time. Also, distal esophageal chemical irritation significantly decreased the pressure amplitude by decreasing the maximum pressure. Following distal esophageal chemical irritation, distal esophageal distention was able to significantly decrease the intercontraction time by decreasing the storage time. However, 3 mL distal colon distention significantly increased the intercontraction time by increasing the storage time. On the other hand, following distal esophageal chemical irritation, electrical stimulation of abdominal branches of vagus nerve did not have any significant effect on intercontraction time. However, electrical stimulation of abdominal branches of vagus nerve significantly increased the pressure amplitude by increasing the maximum pressure. The results of this study demonstrate that urinary bladder function and interaction of bladder with other viscera can be affected by chemical irritation of distal esophagus.

scholarly journals Oxytocin Reduces Intravesical Pressure in Anesthetized Female Rats: Action on Oxytocin Receptors of the Urinary Bladder

2020 ◽  
Vol 11 ◽  
Author(s):  
Eduardo M. Cafarchio ◽  
Luiz A. da Silva ◽  
Luciana C. Auresco ◽  
Itatiana F. Rodart ◽  
Janaina S. de Souza ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Female Rats ◽  
Intravesical Pressure ◽  
Oxytocin Receptors

Cholinergic activation of neurons in the medulla oblongata changes urinary bladder activity by plasma vasopressin release in female rats

2016 ◽  
Vol 776 ◽  
pp. 116-123 ◽  
Author(s):  
Eduardo M. Cafarchio ◽  
Luiz A. da Silva ◽  
Luciana C. Auresco ◽  
Cristiana A. Ogihara ◽  
Roberto L. Almeida ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Medulla Oblongata ◽  
Female Rats ◽  
Vasopressin Release ◽  
Plasma Vasopressin ◽  
Bladder Activity ◽  
Cholinergic Activation

scholarly journals Inhibitory and excitatory perigenital-to-bladder spinal reflexes in the cat

2008 ◽  
Vol 294 (3) ◽  
pp. F591-F602 ◽  
Author(s):  
Changfeng Tai ◽  
Bing Shen ◽  
Jicheng Wang ◽  
Michael B. Chancellor ◽  
James R. Roppolo ◽  
...  
Keyword(s):  
Large Amplitude ◽  
Control Volume ◽  
Spinal Reflexes ◽  
Bladder Function ◽  
Skin Area ◽  
Excitatory Effect ◽  
Noninvasive Methods ◽  
Bladder Activity ◽  
Spinal Cord Injured ◽  
Micturition Volume

This study revealed that in awake chronic spinal cord-injured (SCI) cats reflexes from perigenital skin area to the bladder can be either inhibitory or excitatory. Electrical perigenital stimulation at frequencies between 5 and 7 Hz significantly inhibited large-amplitude rhythmic reflex bladder activity, whereas frequencies between 20 and 40 Hz induced large-amplitude bladder contractions even at low bladder volumes when reflex bladder activity was absent. Both inhibitory and excitatory effects were enhanced as the stimulation intensity increased (5–30 V, 0.2-ms pulse width). During cystometrograms, the inhibitory stimulation (7 Hz) significantly increased the micturition volume threshold 35 ± 13% above the control volume, while the excitatory stimulation (30 Hz) significantly reduced the threshold 21 ± 3%. Mechanical perigenital stimulation applied by repeated light stroking of the perigenital skin with a cotton swab only induced an excitatory effect on the bladder. Both electrical and mechanical perigenital stimuli induced large-amplitude (>30 cmH2O) bladder contractions that were relatively consistent over a range of bladder volumes (10–90% of the capacity). However, the excitatory electrical stimulation only induced bladder contractions lasting on average 42.2 ± 3.9 s, but the mechanical stimulation induced bladder contractions that lasted as long as the stimulation continued (2–3 min). Excitatory electrical or mechanical perigenital stimulation also induced poststimulus voiding. The ability to either inhibit or excite the bladder by noninvasive methods could significantly transform the current clinical management of bladder function after SCI.

scholarly journals Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control

2021 ◽  
Vol 12 ◽  
Author(s):  
Gustavo B. Lamy ◽  
Eduardo M. Cafarchio ◽  
Bárbara do Vale ◽  
Bruno B. Antonio ◽  
Daniel P. Venancio ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Preoptic Area ◽  
Anterior Commissure ◽  
Intravesical Pressure ◽  
Cardiovascular Parameters ◽  
Bladder Control ◽  
Protein Expressions ◽  
Female Wistar Rats ◽  
Urine Storage ◽  
Stimulation Of

Central micturition control and urine storage involve a multisynaptic neuronal circuit for the efferent control of the urinary bladder. Electrical stimulation of the lateral preoptic area (LPA) at the level of the decussation of the anterior commissure in cats evokes relaxation of the bladder, whereas ventral stimulation of LPA evokes vigorous contraction. Endogenous Angiotensin-(1–7) [(Ang-(1–7)] synthesis depends on ACE-2, and its actions on binding to Mas receptors, which were found in LPA neurons. We aimed to investigate the Ang-(1–7) actions into the LPA on intravesical pressure (IP) and cardiovascular parameters. The gene and protein expressions of Mas receptors and ACE-2 were also evaluated in the LPA. Angiotensin-(1–7) (5 nmol/μL) or A-779 (Mas receptor antagonist, 50 nmol/μL) was injected into the LPA in anesthetized female Wistar rats; and the IP, mean arterial pressure (MAP), heart rate (HR), and renal conductance (RC) were recorded for 30 min. Unilateral injection of Ang-(1–7) into the LPA increased IP (187.46 ± 37.23%) with peak response at ∼23–25-min post-injection and yielded no changes in MAP, HR, and RC. Unilateral or bilateral injections of A-779 into the LPA decreased IP (−15.88 ± 2.76 and −27.30 ± 3.40%, respectively) and elicited no changes in MAP, HR, and RC. The genes and the protein expression of Mas receptors and ACE-2 were found in the LPA. Therefore, the LPA is an important part of the circuit involved in the urinary bladder control, in which the Ang-(1–7) synthetized into the LPA activates Mas receptors for increasing the IP independent on changes in RC and cardiovascular parameters.

Comparison of Urinary Bladder Function in 6 and 24 Month Male and Female Rats

1992 ◽  
Vol 148 (5 Part 1) ◽  
pp. 1615-1620 ◽  
Author(s):  
Penelope A. Longhurst ◽  
Berit Eika ◽  
Robert E. Leggett ◽  
Robert M. Levin
Keyword(s):  
Urinary Bladder ◽  
Female Rats ◽  
Bladder Function ◽  
Male And Female ◽  
Male And Female Rats

scholarly journals TRP Channel Agonists Activate Different Afferent Neuromodulatory Mechanisms in Guinea Pig Urinary Bladder

2021 ◽  
Vol 12 ◽  
Author(s):  
Stephanie L. Daugherty ◽  
Jonathan M. Beckel ◽  
Kyoungeun A. Kim ◽  
Bruce A. Freeman ◽  
Jiaxin Liu ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Guinea Pig ◽  
Trp Channels ◽  
Allyl Isothiocyanate ◽  
Inhibitory Effect ◽  
Bladder Function ◽  
Trpc Channels ◽  
Afferent Nerves ◽  
Bladder Activity ◽  
Trpv1 Antagonist

Activation of TRP channels expressed in urinary bladder afferent nerves and urothelium releases neurotransmitters that influence bladder function. Experiments were undertaken to examine the mechanisms underlying effects of TRPA1 (allyl isothiocyanate, AITC), TRPV1 (capsaicin, CAPS), and TRPC (oleoyl-2-acetyl-sn-glycerol, OAG) agonists on guinea pig bladder activity. Effects of these agonists were compared with effects of nitro-oleic acid (OA-NO2), an electrophilic nitro-fatty acid, known to activate TRPV1, TRPA1 or TRPC channels in sensory neurons. AITC (100 μM) increased (231%) area of spontaneous bladder contractions (SBCs) an effect reduced by a TRPA1 antagonist (HC3-03001, HC3, 10 μM) and reversed to inhibition by indomethacin (INDO, 500 nM) a cyclooxygenase inhibitor. The post-INDO inhibitory effect of AITC was mimicked (39% depression) by calcitonin gene-related peptide (CGRP, 100 nM) and blocked by a CGRP antagonist (BIBN, 25 μM). CAPS (1 μM) suppressed SBCs by 30% in 81% of strips, an effect blocked by a TRPV1 antagonist (diarylpiperazine, 1 μM) or BIBN. SBCs were suppressed by OA-NO2 (30 μM, 21% in 77% of strips) or by OAG (50 μM, 30%) an effect blocked by BIBN. OA-NO2 effects were not altered by HC3 or diarylpiperazine. OA-NO2 also induced excitation in 23% of bladder strips. These observations raise the possibility that guinea pig bladder is innervated by at least two types of afferent nerves: [1] Type A express TRPA1 receptors that induce the release of prostaglandins and excite the detrusor, [2] Type B express TRPV1, TRPA1 and TRPC receptors and release CGRP that inhibits the detrusor.

scholarly journals The Ameliorating Effect of Exogenous Melatonin on Urinary Bladder Function in Hyperosmolar Bladder Overactivity and its Influence on the Autonomic Nervous System Activity

2011 ◽  
Vol 54 (2) ◽  
pp. 63-68 ◽  
Author(s):  
Kajetan Juszczak ◽  
Agata Ziomber ◽  
Anna Machowska ◽  
Agata Furgała ◽  
Łukasz Dobrek ◽  
...  
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Urinary Bladder ◽  
Bladder Capacity ◽  
Bladder Function ◽  
Melatonin Treatment ◽  
Motility Index ◽  
Group Iii ◽  
Autonomic Nervous ◽  
Bladder Activity

This study was designed to investigate the effects of melatonin on the bladder hyperactivity in hyperosmolar-induced overactive bladder (OAB) rats. Additionally, the influence of melatonin on the autonomic nervous system (ANS) using heart rate variability (HRV) analysis was assessed. 40 rats were divided into four groups: I – control (n=12), II – rats with hyperosmolar OAB (n=6), III – rats with melatonin pretreatment and hyperosmolar OAB (n=6) and IV – control with melatonin pretreatment (n=6). In group III and IV melatonin in dose of 100 mg/kg was given. HRV measurements in 10 rats, as follow: control (n=2), control after melatonin treatment (n=2), rats with hyperosmolar OAB without (n=3), and after (n=3) melatonin treatment were conducted. This study demonstrates marked influence of melatonin on urinary bladder activity in hyperosmolar-induced OAB rats. These rats showed significantly reduced the detrusor motor overactivity resulting in the improvement of cystometric parameters after melatonin treatment when compared to the control, as follow: a significant increase of intercontraction interval (70 %) and functional bladder capacity (67 %), as well as a decrease of the basal pressure, detrusor overactivity index and motility index of 96 %, 439 % and 40 %, respectively. ANS activity analysis revealed sympathetic overactivity in OAB rats, and parasympathetic superiority in melatonin treated OAB rats. Melatonin treatment in rats with hyperosmolar OAB (group III) caused significant increase of nuHF parameter (from 51.00 ± 25.29 to 76.97 ± 17.43), as well as a decrease of nuLF parameter (from 49.01 ± 25.26 to 23.03 ± 17.43) and LF/HF ratio (from 1.280 ± 0.980 to 0.350 ± 0.330). In conclusion, melatonin suppresses hyperosmolar OAB, and modulates ANS activity by inhibition of the sympathetic drive. Therefore, melatonin may become a useful agent for OAB management.

Modulation of bladder function by prostaglandin EP3 receptors in the central nervous system

2008 ◽  
Vol 295 (4) ◽  
pp. F984-F994 ◽  
Author(s):  
Xin Su ◽  
Lisa A. Leon ◽  
Charlene W. Wu ◽  
Dwight M. Morrow ◽  
Jon-Paul Jaworski ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Urinary Bladder ◽  
Fluorescent Imaging ◽  
Female Rats ◽  
Bladder Function ◽  
Intracerebroventricular Administration ◽  
Afferent Pathways ◽  
Bladder Distension ◽  
The Central Nervous System

Prostaglandin EP3 receptors in the central nervous system (CNS) may exert an excitatory effect on urinary bladder function via modulation of bladder afferent pathways. We have studied this action, using two EP3 antagonists, (2 E)-3-{1-[(2,4-dichlorophenyl)methyl]-5-fluoro-3-methyl-1 H-indol-7-yl}- N-[(4,5-dichloro-2-thienyl)sulfonyl]-2-propenamide (DG041) and (2 E)- N-{[5-bromo-2-(methyloxy)phenyl] sulfonyl}-3-[2-(2-naphthalenylmethyl)phenyl]-2-propenamide (CM9). DG041 and CM9 were proven to be selective EP3 antagonists with radioligand binding and functional fluorescent imaging plate reader (FLIPR) assays. Their effects on volume-induced rhythmic bladder contraction and the visceromotor reflex (VMR) response to urinary bladder distension (UBD) were evaluated in female rats after intrathecal or intracerebroventricular administration. Both DG041 and CM9 showed a high affinity for EP3 receptors at subnanomolar concentrations without significant selectivity for any splice variants. At the human EP3C receptor, both inhibited calcium influx produced by the nonselective agonist PGE2. After intrathecal or intracerebroventricular administration both CM9 and DG041 dose-dependently reduced the frequency, but not the amplitude, of the bladder rhythmic contraction. With intrathecal administration DG041 and CM9 produced a long-lasting and robust inhibition on the VMR response to UBD, whereas with intracerebroventricular injection both compounds elicited only a transient reduction of the VMR response to bladder distension. These data support the concept that EP3 receptors are involved in bladder micturition at supraspinal and spinal centers and in bladder nociception at the spinal cord. A centrally acting EP3 receptor antagonist may be useful in the control of detrusor overactivity and/or pain associated with bladder disorders.

Sign in / Sign up

Export Citation Format

Share Document