scholarly journals A Detailed Spatial Expression Analysis of Wing Phenotypes Reveals Novel Patterns of Odorant Binding Proteins in the Soybean Aphid, Aphis glycines

2021 ◽  
Vol 12 ◽  
Author(s):  
Ling Wang ◽  
Hang Yin ◽  
Zhiguo Zhu ◽  
Shuai Yang ◽  
Jia Fan
Keyword(s):  
Binding Proteins ◽  
Expression Patterns ◽  
Gene Families ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Rapid Expansion ◽  
Odorant Binding Proteins ◽  
Spatial Expression ◽  
Wide Range ◽  
Odorant Binding

The wide range of insect niches has led to a rapid expansion of chemosensory gene families as well as their relatively independent evolution and a high variation. Previous studies have revealed some functions for odorant-binding proteins (OBPs) in processes beyond olfaction, such as gustation and reproduction. In this study, a comparative transcriptomic analysis strategy was applied for the soybean aphid, Aphis glycines, focusing on various functional tissues and organs of winged aphids, including the antenna, head, leg, wing, thorax, cauda, and cornicle. Detailed spatial OBP expression patterns in winged and wingless parthenogenetic aphids were detected by RT-qPCR. Twelve OBPs were identified, and three new OBPs in A. glycines are first reported. All OBPs showed comparatively higher expression in sensory organs and tissues, such as the antenna, head, or leg. Additionally, we found some novel expression patterns for aphid OBPs (Beckendorf et al., 2008). Five OBPs exhibited high-expression levels in the cauda and four in the cornicle (Biasio et al., 2015). Three genes (OBP2/3/15) were highly expressed in the wing (Calvello et al., 2003). Two (OBP3/15) were significantly more highly expressed in the wingless thorax than in the winged thorax with the wings removed, and these transcripts were significantly enriched in the removed wings. More details regarding OBP spatial expression were revealed under our strategy. These findings supported the existence of carrier transport functions other than for foreign chemicals and therefore broader ligand ranges of aphid OBPs. It is important for understanding how insect OBPs function in chemical perception as well as their other potential physiological functions.

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

2019 ◽  
Vol 27 (5) ◽  
pp. 1019-1030 ◽  
Author(s):  
Ling Wang ◽  
Ying‐Dong Bi ◽  
Ming Liu ◽  
Wei Li ◽  
Miao Liu ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Expression Profiles ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Odorant Binding Proteins ◽  
Odorant Binding

Expression patterns of odorant-binding proteins in antennae of the moth Manduca sexta

2003 ◽  
Vol 313 (3) ◽  
pp. 321-333 ◽  
Author(s):  
James B. Nardi ◽  
Lou Ann Miller ◽  
Kimberly K. O. Walden ◽  
Susan Rovelstad ◽  
Liping Wang ◽  
...  
Keyword(s):  
Manduca Sexta ◽  
Binding Proteins ◽  
Expression Patterns ◽  
Odorant Binding Proteins ◽  
Odorant Binding

scholarly journals Odorant-binding proteins in canine anal sac glands indicate an evolutionarily conserved role in mammalian chemical communication

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sunita Janssenswillen ◽  
Kim Roelants ◽  
Sebastien Carpentier ◽  
Hilde de Rooster ◽  
Mieke Metzemaekers ◽  
...  
Keyword(s):  
Chemical Communication ◽  
Binding Proteins ◽  
Evolutionary Relationship ◽  
Gene Clusters ◽  
Pseudoautosomal Region ◽  
Comparative Genomic ◽  
Odorant Binding Proteins ◽  
Wide Range ◽  
Anal Sac ◽  
Odorant Binding

Abstract Background Chemical communication is an important aspect of the behavioural ecology of a wide range of mammals. In dogs and other carnivores, anal sac glands are thought to convey information to conspecifics by secreting a pallet of small volatile molecules produced by symbiotic bacteria. Because these glands are unique to carnivores, it is unclear how their secretions relate to those of other placental mammals that make use of different tissues and secretions for chemical communication. Here we analyse the anal sac glands of domestic dogs to verify the secretion of proteins and infer their evolutionary relationship to those involved in the chemical communication of non-carnivoran mammals. Results Proteomic analysis of anal sac gland secretions of 17 dogs revealed the consistently abundant presence of three related proteins. Homology searches against online databases indicate that these proteins are evolutionary related to ‘odorant binding proteins’ (OBPs) found in a wide range of mammalian secretions and known to contribute to chemical communication. Screening of the dog’s genome sequence show that the newly discovered OBPs are encoded by a single cluster of three genes in the pseudoautosomal region of the X-chromosome. Comparative genomic screening indicates that the same locus is shared by a wide range of placental mammals and that it originated at least before the radiation of extant placental orders. Phylogenetic analyses suggest a dynamic evolution of gene duplication and loss, resulting in large gene clusters in some placental taxa and recurrent loss of this locus in others. The homology of OBPs in canid anal sac glands and those found in other mammalian secretions implies that these proteins maintained a function in chemical communication throughout mammalian evolutionary history by multiple shifts in expression between secretory tissues involved in signal release and nasal mucosa involved in signal reception. Conclusions Our study elucidates a poorly understood part of the biology of a species that lives in close association with humans. In addition, it shows that the protein repertoire underlying chemical communication in mammals is more evolutionarily stable than the variation of involved glands and tissues would suggest.

scholarly journals Odorant-binding proteins expression patterns in recently diverged species of Anastrepha fruit flies

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Emeline Boni Campanini ◽  
Carlos Congrains ◽  
Felipe Rafael Torres ◽  
Reinaldo Alves de Brito
Keyword(s):  
Binding Proteins ◽  
Expression Patterns ◽  
Fruit Flies ◽  
Odorant Binding Proteins ◽  
Odorant Binding

scholarly journals Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7054 ◽  
Author(s):  
Angélica González-González ◽  
María E. Rubio-Meléndez ◽  
Gabriel I. Ballesteros ◽  
Claudio C. Ramírez ◽  
Rubén Palma-Millanao
Keyword(s):  
Binding Proteins ◽  
Expression Patterns ◽  
Scarab Beetle ◽  
Control Methods ◽  
Specific Expression ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Qrt Pcr ◽  
Odorant Binding ◽  
Pest Control Methods

In this study, we addressed the sex- and tissue-specific expression patterns of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in Hylamorpha elegans (Burmeister), an important native scarab beetle pest species from Chile. Similar to other members of its family, this scarab beetle exhibit habits that make difficult to control the pest by conventional methods. Hence, alternative ways to manage the pest populations based on chemical communication and signaling (such as disrupting mating or host finding process) are highly desirable. However, developing pest-control methods based on chemical communication requires to understand the molecular basis for pheromone recognition/chemical perception in this species. Thus, with the aim of discovering olfaction-related genes, we obtained the first reference transcriptome assembly of H. elegans. We used different tissues of adult beetles from males and females: antennae and maxillary palps, which are well known for embedded sensory organs. Then, the expression of predicted odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) was analyzed by qRT-PCR. In total, 165 transcripts related to chemoperception were predicted. Of these, 16 OBPs, including one pheromone-binding protein (PBP), and four CSPs were successfully amplified by qRT-PCR. All of these genes were differentially expressed in the sensory tissues with respect to the tibial tissue that was used as a control. The single predicted PBP found was highly expressed in the antennal tissues, particularly in males, while several OBPs and one CSP showed male-biased expression patterns, suggesting that these proteins may participate in sexual recognition process. In addition, a single CSP was expressed at higher levels in female palps than in any other studied condition, suggesting that this CSP would participate in oviposition process. Finally, all four CSPs exhibited palp-biased expression while mixed results were obtained for the expression of the OBPs, which were more abundant in the palps than in the antennae. These results suggest that these chemoperception proteins would be interesting novel targets for control of H. elegans, thus providing a theoretical basis for further studies involving new pest control methods.

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