scholarly journals Optimization of Ammonium Sulfate Concentration for Purification of Colorectal Cancer Vaccine Candidate Recombinant Protein GA733-FcK Isolated from Plants

2015 ◽  
Vol 6 ◽  
Author(s):  
Se-Ra Park ◽  
Chae-Yeon Lim ◽  
Deuk-Su Kim ◽  
Kisung Ko
Keyword(s):  
Colorectal Cancer ◽  
Recombinant Protein ◽  
Ammonium Sulfate ◽  
Cancer Vaccine ◽  
Vaccine Candidate ◽  
Sulfate Concentration

Functionality of insect-cell-derived colorectal cancer vaccine candidate protein EpCAM-Fc in human dendritic cells

2015 ◽  
Vol 45 (3) ◽  
pp. 162-166 ◽  
Author(s):  
Yo Seph Park ◽  
Jeong-Hwan Lee ◽  
Jin-Yeon Park ◽  
Han Sung Hwang ◽  
Martin Zenke ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dendritic Cells ◽  
Cancer Vaccine ◽  
Insect Cell ◽  
Vaccine Candidate ◽  
Candidate Protein ◽  
Human Dendritic Cells

scholarly journals Effect of leaf position and days post-infiltration on transient expression of colorectal cancer vaccine candidate proteins GA733-Fc and GA733-FcK in Nicotiana benthamiana plant

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10851
Author(s):  
Kibum Kim ◽  
Yang Joo Kang ◽  
Se Ra Park ◽  
Do-Sun Kim ◽  
Seung-Won Lee ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Vaccine ◽  
Nicotiana Benthamiana ◽  
Vaccine Candidate ◽  
Fusion Gene ◽  
Protein A ◽  
Leaf Position ◽  
Plant Expression Vector ◽  
Promising Strategy ◽  
Expression Time

Immunization with thetumor-associated antigen GA733 glycoprotein, which is highly expressed in colorectal cancer, is considered to be a promising strategy for cancer prevention and treatment. We cloned a fusion gene of GA733 and immunoglobulin Fc fragment (GA733-Fc), and that of GA733-Fc and an endoplasmic reticulum retention motif (GA733-FcK) into the Cowpea mosaic virus (CPMV)-based transient plant expression vector, pEAQ-HT. Agrobacterium tumefaciens (LBA4404) transformed with the vectors pEAQ-HT-GA733-Fc and pEAQ-HT-GA733-FcK was infiltrated into the leaves of Nicotiana benthamiana plants. To optimize harvesting of leaf to express therapeutic glycoproteins both spatially and temporally, protein expression levels at various leaf positions (top, middle, and base) and days post-infiltration (dpi) were investigated. The GA733-Fc and GA733-FcK genes were detected in leaves at 1–10 dpi using PCR. As assessed by western blot, GA733-Fc and GA733-FcK were expressed at the highest levels in the top leaf position at 5 dpi, and GA733-FcK was expressed more than GA733-Fc. The proteins were successfully purified from infiltrated N. benthamiana leaves using protein A affinity chromatography. ELISA verified that an anti-GA733 antibody recognized both purified proteins. Thus, a functional GA733-Fc colorectal cancer vaccine protein can be transiently expressed using a CPMV virus-based vector, with an optimized expression time and leaf position post-infiltration.

scholarly journals Immunoinformatics Study on Early 4 Protein of Human Papillomavirus Type 16 for Cervical Cancer Vaccine Peptide Candidate

2019 ◽  
Vol 4 (2) ◽  
pp. 252-262
Author(s):  
Yani Suryani ◽  
Opik Taupiqurrohman ◽  
Muhammad Yusuf ◽  
Toto Subroto ◽  
Sukma Nuswantara
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Amino Acid ◽  
Cancer Vaccine ◽  
Vaccine Candidate ◽  
Peptide Vaccine ◽  
Human Papillomavirus Type 16 ◽  
Cervical Cancer Vaccine ◽  
Peptide Candidate

 The aims of this study were to carry out testing of the early 4 protein of type 16 HPV through immunoinformatics meth-ods in an effort to get the peptide vaccine candidate for cervical cancer. The software used are IEDB-AR, CABSdock and Accelrys Discovery Study 4.5. Based on the analysis that sequence of ami-no acid lysine, leucine, leucine, glycine, serine, threonine, tryp-tophan, proline and threonine (KLLGSTWPT) and the sequence of amino acid tyrosine, tyrosine, valine, leucine, histidine, leucine, cysteine, leucine, alanine, alanine, threonine, lysine, tyrosine, pro-line and leucine (YYVLHLCLAATKYPL) are peptide vaccine can-didate for cervical cancer from the early 4 protein of HPV type 16 

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