scholarly journals Genetic Mapping and Discovery of the Candidate Gene for Black Seed Coat Color in Watermelon (Citrullus lanatus)

2020 ◽  
Vol 10 ◽  
Author(s):  
Bingbing Li ◽  
Xuqiang Lu ◽  
Haileslassie Gebremeskel ◽  
Shengjie Zhao ◽  
Nan He ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Candidate Gene ◽  
Seed Coat ◽  
Coat Color ◽  
Citrullus Lanatus ◽  
Seed Coat Color ◽  
Black Seed ◽  
Black Seed Coat

Biochemical and molecuar marker based screening of seed longevity in soybean [Glycine max ( L.) Merill ]

2018 ◽  
Author(s):  
P. V. Pawar ◽  
R. M. Naik ◽  
M. P. Deshmukh ◽  
R. D. Satbhai ◽  
S. G. Mohite
Keyword(s):  
Lipid Peroxidation ◽  
Seed Coat ◽  
Coat Color ◽  
Soybean Seed ◽  
Coat Colour ◽  
Seed Longevity ◽  
Seed Coat Color ◽  
Seed Coat Colour ◽  
Black Seed ◽  
Black Seed Coat

The soybean seed is highly susceptible to field weathering and mechanical damage which adversely affect its longevity. Mechanical injury can occur at any time during harvesting, drying and storage conditioning of seeds. The seed coat color and leachate conductivity of soybean has been correlated with seed longevity and black seed coat color has been reported to be positively correlated with better seed longevity. In order to understand the physico-chemical attributes related to soybean seed longevity, biochemical and molecular analysis of the parents exhibiting black (Birsasoya-1) and yellow seed coat colour (EC 241780) and the eleven F3 progenies of the cross exhibiting brown, yellow and black seed coat colour was carried out. The results revealed that vita-E, lignin, calcium content and activity of antioxidative enzymes appeared to be positively correlated with soybean seed longevity and levels were higher in black and brown seed coat color progenies. The lipid peroxidation rate was inversely related to membrane injury caused by ROS and comparatively much less lipid peroxidation rate was recorded in black and brown seed coat colour parents and progenies having better seed longevity. The SSR primers Satt162, Satt523 and Satt453 which are either linked with seed coat colour and seed permeability exhibited a specific size allelic fragments in soybean genotypes and crosses with better seed longevity.

scholarly journals Breeding of Black Soybean with Green Cotyledon and Four Recessive Alleles for Lipoxygenase, Kunitz Trypsin Inhibitor, Lectin, and Stachyose

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 309
Author(s):  
Sang Woo Choi ◽  
Jae Eun Kang ◽  
Seong Kyeong Lee ◽  
Sarath Ly ◽  
Jong Il Chung
Keyword(s):  
Seed Coat ◽  
Coat Color ◽  
Soybean Seed ◽  
Breeding Line ◽  
Seed Coat Color ◽  
Kunitz Trypsin Inhibitor ◽  
Black Soybean ◽  
Black Seed ◽  
Green Cotyledon ◽  
Black Seed Coat

Anthocyanins from the black soybean seed coat are known to have many pharmaceutical effects. However, black soybean seed contains antinutritional factors such as lipoxygenase, Kunitz trypsin inhibitor (KTI), lectin, and stachyose. The genetic removal of these components will improve the nutritional value of black soybean seed. The objective of this research was to breed a soybean strain with the black seed coat color, green cotyledon color, and tetra recessive allele (lox1lox2lox3/lox1lox2lox3-ti/ti-le/le-rs2/rs2) for lipoxygenase, KTI, lectin, and stachyose components. Eight parents were used to breed the tetra null strain. Analysis of lipoxygenase, KTI, lectin, and stachyose components in mature seeds was conducted by SDS-PAGE, Western blot, and HPLC. The soybean line with the black seed coat color, the green cotyledon color, a large seed size, and tetra recessive alleles has purple flowers, a determinate growth habit, and brown pods at maturity. The stem height of the breeding line was 52.3 cm. The 100-seed weight of the breeding line was 35.2 g and the yield (Ton/ha) was 2.50. The stachyose content of the breeding line was 3.30 g/kg. This is the first soybean strain with the black seed coat color, the green cotyledon color, a large seed size, and tetra null alleles (lox1lox2lox3/lox1lox2lox3-ti/ti-le/le-rs2/rs2, low content of stachyose, free of lipoxygenase, KTI, and lectin proteins).

scholarly journals Identification of candidate genes controlling black seed coat and pod tip color in cowpea (Vigna unguiculata L. Walp)

2018 ◽  
Author(s):  
Ira A Herniter ◽  
María Muñoz-Amatriaín ◽  
Sassoum Lo ◽  
Yi-Ning Guo ◽  
Timothy J Close
Keyword(s):  
Candidate Genes ◽  
Vigna Unguiculata ◽  
Seed Coat ◽  
Coat Color ◽  
Anthocyanin Biosynthesis ◽  
Biosynthesis Pathway ◽  
Seed Coat Color ◽  
Black Seed ◽  
Black Seed Coat ◽  
Myb Domain

ABSTRACTSeed coat color is an important part of consumer preferences for cowpea (Vigna unguiculata L. Walp). Color has been studied in numerous crop species and has often been linked to loci controlling the anthocyanin biosynthesis pathway. This study makes use of available resources, including mapping populations, a reference genome, and a high-density single nucleotide polymorphism genotyping platform, to map the black seed coat and purple pod tip color traits in cowpea. Several gene models encoding MYB domain protein 113 were identified as candidate genes. MYB domain proteins have been shown in other species to control expression of genes encoding enzymes for the final steps in the anthocyanin biosynthesis pathway. PCR analysis indicated that a presence/absence variation of one or more MYB113 genes may control the presence or absence of black pigment. A PCR marker has been developed for black seed coat color in cowpea.

scholarly journals The inhibitory effects of polyphenolic compounds on the damage caused by safflower fly (Acanthiophilus helianthi) in Carthamus spp.

2020 ◽  
Vol 13 (2) ◽  
pp. 87-93
Author(s):  
Karami Soraya ◽  
Basaki Tayebeh ◽  
Amin Mousavi Khaneghah
Keyword(s):  
Ferulic Acid ◽  
Seed Coat ◽  
Coat Color ◽  
Block Design ◽  
Negative Relationship ◽  
Polyphenolic Compounds ◽  
Seed Coat Color ◽  
Black Seed ◽  
Significant Difference ◽  
Black Seed Coat

Seed coat color is probably a determinant factor in the antibiosis mechanism of developing resistance to safflower fly (Acanthiophilus helianthi). The purpose of this study was to determine the relationship between the phytochemical content of safflower (Carthamus spp.) seed coat extract with the seed coat color and the damage caused by safflower fly. To this end, germplasm consisting of the cultivated species (C111, with a white seed coat), the wild species (Glaucus and lanatus with a black seed coat; Azar with a brown seed coat), and a breeding line (A82 with a black seed coat) was formed. After cultivating the genotypes, the seed loss (%) and its relationship with the polyphenolic compounds and Cyanidin-3-glucoside of the seed coat extract were examined. Agricultural and phytochemical trait data were analyzed through a completely random block design. With a significant difference from other samples, a minimum damage percentage was observed in Lanatus, Glaucus, and A82 genotypes with a black coat (P < 0.05). The concentration of phenolic compounds, that is, chlorogenic acid, caffeic acid, and p-coumaric acid, except for ferulic acid, was almost equal in all genotypes. However, there was an inverse and direct relationship between the concentration of four polyphenolic compounds (rutin, apigenin, quercetin, and ferulic acid) and Cyd-3-glu content with resistance safflower fly, respectively. In general, flavonoid compounds, that is, rutin, quercetin, and apigenin, affect the resistance probably through antibiosis mechanism so that there was a negative relationship between the concentration of these compounds and resistance to safflower fly.

scholarly journals Mapping and identification of yellow seed coat color genes in Brassica juncea L.

2020 ◽  
Author(s):  
Zhen Huang ◽  
Yang Wang ◽  
Hong Lu ◽  
Xiang Liu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Seed Coat Color ◽  
Flavonoid Pathway ◽  
Yellow Seed ◽  
Color Formation ◽  
Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

scholarly journals Fine-Mapping And Identification of A Candidate Gene Controlling Seed Coat Color In Melon (Cucumis Melo L. Var. Chinensis Pangalo)

2021 ◽  
Author(s):  
Zhicheng Hu ◽  
Xueyin Shi ◽  
Xuemiao Chen ◽  
Jing Zheng ◽  
Aiai Zhang ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Seed Coat ◽  
Coat Color ◽  
Bulked Segregant Analysis ◽  
Dominant Gene ◽  
Seed Coat Color ◽  
Gene Encoding ◽  
Yellow Seed ◽  
Homeobox Protein ◽  
Yellow Seed Coat

Abstract Seed coat color is related to flavonoid content which is closely related to seed dormancy. According to the genetic analysis of a six-generation population derived from two parents (IC2508 with a yellow seed coat and IC2518 with a brown seed coat), we discovered that the yellow seed coat trait in melon was controlled by a single dominant gene, named CmBS-1. Bulked segregant analysis sequencing (BSA-Seq) revealed that the gene was located at 11,860,000–15,890,000 bp (4.03 Mb) on Chr 6. The F2 population was genotyped using insertion-deletions (InDels), from which cleaved amplified polymorphic sequence (dCAPS) markers were derived to construct a genetic map. The gene was then fine-mapped to a 233.98 kb region containing 12 genes. Based on gene sequence analysis with two parents, we found that the MELO3C019554 gene encoding a homeobox protein (PHD transcription factor) had a nonsynonymous single nucleotide polymorphism (SNP) mutation in the coding sequence (CDS), and the SNP mutation resulted in the conversion of an amino acid (A→T) at residue 534. In addition, MELO3C019554 exhibited lower relative expression levels in the yellow seed coat than in the brown seed coat. Furthermore, we found that MELO3C019554 was related to 12 flavonoid metabolites. Thus, we predicted that MELO3C019554 is a candidate gene controlling seed coat color in melon. The study lays a foundation for further cloning projects and functional analysis of this gene, as well as marker-assisted selection breeding.

Transcriptome Analysis of Black and White Sesame Seed Reveals Candidate Genes Associated with Black Seed Development in Sesame (Sesamum Indicum)

2020 ◽  
Author(s):  
Senouwa Segla Koffi Dossou ◽  
Linhai Wang ◽  
Xin Wei ◽  
Yanxin Zhang ◽  
Donghua Li ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Seed Development ◽  
Seed Coat ◽  
Coat Color ◽  
Sesame Seed ◽  
Sesamum Indicum ◽  
Seed Coat Color ◽  
Seed Formation ◽  
Black And White ◽  
Black Seed

Abstract Background: Seed coat color is a key agronomic characteristic in sesame (Sesamum indicum) since it is strongly linked to seed oil, proteins, and lignans content and it influences consumer preferences. Even though some QTL and candidate genes have been detected for sesame seed coat color, the mechanism and regulation of black pigmentation are not entirely understood. This study provides an overview of developing seeds transcriptome of two varieties of sesame “Zhongfengzhi No.1” (white seed) and “Zhongzhi No.33” (black seed) and shed light on genes involving in black seed formation.Results: Both black and white sesame showed similar trend expressed genes with the numbers increased at the early stages of seed development. The differentially expressed genes (DEGs) number increased with seed development in the two sesame varieties. We examined the DEGs and uncovered that the early stage, which is from 8 to 17 days post-anthesis (DPA) plays an important role in black pigment biosynthesis and accumulation. The gene expression patterns were consistent with the seed color change. Besides, we studied the shared DEGs between the black and white sesame. We figured out 17 candidate genes associated with pigments biosynthesis in black sesame seed including 2 chalcone synthase genes SIN_1018961 and SIN_1018959 which may function in the phenylpropanoid pathway. 5 of these candidate genes, SIN_1006242 and SIN_1016759/PPO, SIN_1026689 and SIN_1006025, SIN_1025056 are located on chromosomes 4, 8 and 11 respectively, in conformity with previous QTL mapping. These genes were believed to play a major role in black seed development in sesame. Conclusion: This work illuminated the different expression profiles in black and white sesames and unfolded pivotal stages and a catalog of candidate genes associated with black seed formation in sesame. These findings provide a vast transcriptome dataset and list of genes that will be targeted for functional studies related to the molecular mechanism involved in biosynthesis and regulation of seed coat color in sesame and for molecular breeding of high-quality sesame varieties.

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