Biochemical and molecuar marker based screening of seed longevity in soybean [Glycine max ( L.) Merill ]

2018 ◽  
Author(s):  
P. V. Pawar ◽  
R. M. Naik ◽  
M. P. Deshmukh ◽  
R. D. Satbhai ◽  
S. G. Mohite
Keyword(s):  
Lipid Peroxidation ◽  
Seed Coat ◽  
Coat Color ◽  
Soybean Seed ◽  
Coat Colour ◽  
Seed Longevity ◽  
Seed Coat Color ◽  
Seed Coat Colour ◽  
Black Seed ◽  
Black Seed Coat

The soybean seed is highly susceptible to field weathering and mechanical damage which adversely affect its longevity. Mechanical injury can occur at any time during harvesting, drying and storage conditioning of seeds. The seed coat color and leachate conductivity of soybean has been correlated with seed longevity and black seed coat color has been reported to be positively correlated with better seed longevity. In order to understand the physico-chemical attributes related to soybean seed longevity, biochemical and molecular analysis of the parents exhibiting black (Birsasoya-1) and yellow seed coat colour (EC 241780) and the eleven F3 progenies of the cross exhibiting brown, yellow and black seed coat colour was carried out. The results revealed that vita-E, lignin, calcium content and activity of antioxidative enzymes appeared to be positively correlated with soybean seed longevity and levels were higher in black and brown seed coat color progenies. The lipid peroxidation rate was inversely related to membrane injury caused by ROS and comparatively much less lipid peroxidation rate was recorded in black and brown seed coat colour parents and progenies having better seed longevity. The SSR primers Satt162, Satt523 and Satt453 which are either linked with seed coat colour and seed permeability exhibited a specific size allelic fragments in soybean genotypes and crosses with better seed longevity.

Verification of Soybean Seed Coat Colour Specific Markers Reveals I Loci Specific Markers Capable for Distinguishing Genotypes Differing in Seed Coated Colour

2020 ◽  
Author(s):  
R. B. Shingare ◽  
V. P. Chimote ◽  
M. P. Deshmukh ◽  
T. J. Bhor ◽  
A. A. Kale
Keyword(s):  
Seed Coat ◽  
Coat Color ◽  
Soybean Seed ◽  
Coat Colour ◽  
Specific Primers ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Black Seed ◽  
Soybean Seed Coat ◽  
Yellow Seed Coat

Background: In soybean yellow seed coat is preferred in the market, however, colored ones are currently gaining attention because of their medicinal and nutritive values; besides. Hence it is essential to breed varieties with desired seed coat colour. Methods: Twelve genotypes with six each having yellow and black seed coats were screened with fourteen primers linked to seed coat colour governing loci. Result: Out of them twelve primers showed polymorphism. Monomorphism was observed with both T loci specific and two of the three R loci specific primers. However I locus specific primers i.e. SM303, SM305 and TR showed polymorphism shared by their seed coat color. SM303 amplified a 180 bp sized band in yellow seed coated genotypes and a 130 bp band in black seed coated genotypes. SM305 amplified dual bands with a 200bp band being monomorphic and an additional band (192-216 bp range) present in only yellow seed coated genotypes, of which a 208 bp band was shared by four yellow seed coated genotypes. Cold induced seed coat discoloration specific TR primer generated bands of different size ranges in yellow seed coated (336-344 bp) and black seed coated genotypes (300-320), of which a 340 bp band was shared by four yellow seed coated genotypes.

scholarly journals Breeding of Black Soybean with Green Cotyledon and Four Recessive Alleles for Lipoxygenase, Kunitz Trypsin Inhibitor, Lectin, and Stachyose

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 309
Author(s):  
Sang Woo Choi ◽  
Jae Eun Kang ◽  
Seong Kyeong Lee ◽  
Sarath Ly ◽  
Jong Il Chung
Keyword(s):  
Seed Coat ◽  
Coat Color ◽  
Soybean Seed ◽  
Breeding Line ◽  
Seed Coat Color ◽  
Kunitz Trypsin Inhibitor ◽  
Black Soybean ◽  
Black Seed ◽  
Green Cotyledon ◽  
Black Seed Coat

Anthocyanins from the black soybean seed coat are known to have many pharmaceutical effects. However, black soybean seed contains antinutritional factors such as lipoxygenase, Kunitz trypsin inhibitor (KTI), lectin, and stachyose. The genetic removal of these components will improve the nutritional value of black soybean seed. The objective of this research was to breed a soybean strain with the black seed coat color, green cotyledon color, and tetra recessive allele (lox1lox2lox3/lox1lox2lox3-ti/ti-le/le-rs2/rs2) for lipoxygenase, KTI, lectin, and stachyose components. Eight parents were used to breed the tetra null strain. Analysis of lipoxygenase, KTI, lectin, and stachyose components in mature seeds was conducted by SDS-PAGE, Western blot, and HPLC. The soybean line with the black seed coat color, the green cotyledon color, a large seed size, and tetra recessive alleles has purple flowers, a determinate growth habit, and brown pods at maturity. The stem height of the breeding line was 52.3 cm. The 100-seed weight of the breeding line was 35.2 g and the yield (Ton/ha) was 2.50. The stachyose content of the breeding line was 3.30 g/kg. This is the first soybean strain with the black seed coat color, the green cotyledon color, a large seed size, and tetra null alleles (lox1lox2lox3/lox1lox2lox3-ti/ti-le/le-rs2/rs2, low content of stachyose, free of lipoxygenase, KTI, and lectin proteins).

scholarly journals Genetic Mapping and Discovery of the Candidate Gene for Black Seed Coat Color in Watermelon (Citrullus lanatus)

2020 ◽  
Vol 10 ◽  
Author(s):  
Bingbing Li ◽  
Xuqiang Lu ◽  
Haileslassie Gebremeskel ◽  
Shengjie Zhao ◽  
Nan He ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Candidate Gene ◽  
Seed Coat ◽  
Coat Color ◽  
Citrullus Lanatus ◽  
Seed Coat Color ◽  
Black Seed ◽  
Black Seed Coat

scholarly journals VaSDC1 Is Involved in Modulation of Flavonoid Metabolic Pathways in Black and Red Seed Coats in Adzuki Bean (Vigna angularis L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Liwei Chu ◽  
Pu Zhao ◽  
Kaili Wang ◽  
Bo Zhao ◽  
Yisong Li ◽  
...  
Keyword(s):  
Seed Coat ◽  
Metabolic Pathways ◽  
Nutritional Quality ◽  
Coat Colour ◽  
Myb Transcription Factor ◽  
Adzuki Bean ◽  
Vigna Angularis ◽  
Seed Coat Colour ◽  
Black Seed ◽  
Black Seed Coat

Seed coat colour is an important nutritional quality trait. Variations in anthocyanins and flavonoids induce the diversity of seed coat colour in adzuki bean (Vigna angularis L.). Red seed coat and black seed coat are important adzuki bean cultivars. Insights into the differences of flavonoid metabolic pathways between black and red adzuki bean are significant. In this study, we explored that the difference in seed coat colour between the red (Jingnong6) and the black (AG118) is caused by the accumulation of anthocyanins. The RNA-sequencing (RNA-Seq) and real-time reverse transcription (qRT)-PCR results showed that the Vigna angularis L. seed coat color (VaSDC1) gene, an R2R3-MYB transcription factor, should be the key gene to regulate the black and red seed coat colours. In three different colouring staes of seed development, VaSDC1 was specifically expressed in the black seed coat (AG118) landrace, which activates the structural genes of flavonoid metabolic pathways. As a result, this caused a substantial accumulation of anthocyanins and created a dark blue-black colour. In the red (Jingnong6) seed coat variety, low expression levels of VaSDC1 resulted in a lower accumulation of anthocyanins than in AG118. In addition, VaSDC1 was genetically mapped in the interval between simple-sequence repeat (SSR) markers Sca326-12, Sca326-4, and BAgs007 on chromosome 3 using an F4 segregating population derived from the cross between Jingnong6 and AG118. These results will facilitate the improvement of nutritional quality breeding in adzuki beans.

scholarly journals Identification of candidate genes controlling black seed coat and pod tip color in cowpea (Vigna unguiculata L. Walp)

2018 ◽  
Author(s):  
Ira A Herniter ◽  
María Muñoz-Amatriaín ◽  
Sassoum Lo ◽  
Yi-Ning Guo ◽  
Timothy J Close
Keyword(s):  
Candidate Genes ◽  
Vigna Unguiculata ◽  
Seed Coat ◽  
Coat Color ◽  
Anthocyanin Biosynthesis ◽  
Biosynthesis Pathway ◽  
Seed Coat Color ◽  
Black Seed ◽  
Black Seed Coat ◽  
Myb Domain

ABSTRACTSeed coat color is an important part of consumer preferences for cowpea (Vigna unguiculata L. Walp). Color has been studied in numerous crop species and has often been linked to loci controlling the anthocyanin biosynthesis pathway. This study makes use of available resources, including mapping populations, a reference genome, and a high-density single nucleotide polymorphism genotyping platform, to map the black seed coat and purple pod tip color traits in cowpea. Several gene models encoding MYB domain protein 113 were identified as candidate genes. MYB domain proteins have been shown in other species to control expression of genes encoding enzymes for the final steps in the anthocyanin biosynthesis pathway. PCR analysis indicated that a presence/absence variation of one or more MYB113 genes may control the presence or absence of black pigment. A PCR marker has been developed for black seed coat color in cowpea.

scholarly journals Inheritance of black seed coat colour in lentil

2020 ◽  
Author(s):  
G.N. Suvorova ◽  
Keyword(s):  
Seed Coat ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Black Seed ◽  
Black Seeds ◽  
Grey Colour ◽  
Black Seed Coat ◽  
Dominant Genes

The purpose of the research was to determine the inheritance of black seed coat colour in lentil variety ‘Beluga’. The seeds collected from F1 plants in cross of ‘Rauza’ (yellow seeds) × ‘Beluga’ (black seeds) were of grey colour with black mottles. F2 ratio of nonblack and black seeds was 62:6, which corresponded to 15:1 dihybrid segregation. It is concluded that the black seed coat colour of ‘Beluga’ is controlled by two dominant genes.

scholarly journals The inhibitory effects of polyphenolic compounds on the damage caused by safflower fly (Acanthiophilus helianthi) in Carthamus spp.

2020 ◽  
Vol 13 (2) ◽  
pp. 87-93
Author(s):  
Karami Soraya ◽  
Basaki Tayebeh ◽  
Amin Mousavi Khaneghah
Keyword(s):  
Ferulic Acid ◽  
Seed Coat ◽  
Coat Color ◽  
Block Design ◽  
Negative Relationship ◽  
Polyphenolic Compounds ◽  
Seed Coat Color ◽  
Black Seed ◽  
Significant Difference ◽  
Black Seed Coat

Seed coat color is probably a determinant factor in the antibiosis mechanism of developing resistance to safflower fly (Acanthiophilus helianthi). The purpose of this study was to determine the relationship between the phytochemical content of safflower (Carthamus spp.) seed coat extract with the seed coat color and the damage caused by safflower fly. To this end, germplasm consisting of the cultivated species (C111, with a white seed coat), the wild species (Glaucus and lanatus with a black seed coat; Azar with a brown seed coat), and a breeding line (A82 with a black seed coat) was formed. After cultivating the genotypes, the seed loss (%) and its relationship with the polyphenolic compounds and Cyanidin-3-glucoside of the seed coat extract were examined. Agricultural and phytochemical trait data were analyzed through a completely random block design. With a significant difference from other samples, a minimum damage percentage was observed in Lanatus, Glaucus, and A82 genotypes with a black coat (P < 0.05). The concentration of phenolic compounds, that is, chlorogenic acid, caffeic acid, and p-coumaric acid, except for ferulic acid, was almost equal in all genotypes. However, there was an inverse and direct relationship between the concentration of four polyphenolic compounds (rutin, apigenin, quercetin, and ferulic acid) and Cyd-3-glu content with resistance safflower fly, respectively. In general, flavonoid compounds, that is, rutin, quercetin, and apigenin, affect the resistance probably through antibiosis mechanism so that there was a negative relationship between the concentration of these compounds and resistance to safflower fly.

Transcriptome Analysis of Black and White Sesame Seed Reveals Candidate Genes Associated with Black Seed Development in Sesame (Sesamum Indicum)

2020 ◽  
Author(s):  
Senouwa Segla Koffi Dossou ◽  
Linhai Wang ◽  
Xin Wei ◽  
Yanxin Zhang ◽  
Donghua Li ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Seed Development ◽  
Seed Coat ◽  
Coat Color ◽  
Sesame Seed ◽  
Sesamum Indicum ◽  
Seed Coat Color ◽  
Seed Formation ◽  
Black And White ◽  
Black Seed

Abstract Background: Seed coat color is a key agronomic characteristic in sesame (Sesamum indicum) since it is strongly linked to seed oil, proteins, and lignans content and it influences consumer preferences. Even though some QTL and candidate genes have been detected for sesame seed coat color, the mechanism and regulation of black pigmentation are not entirely understood. This study provides an overview of developing seeds transcriptome of two varieties of sesame “Zhongfengzhi No.1” (white seed) and “Zhongzhi No.33” (black seed) and shed light on genes involving in black seed formation.Results: Both black and white sesame showed similar trend expressed genes with the numbers increased at the early stages of seed development. The differentially expressed genes (DEGs) number increased with seed development in the two sesame varieties. We examined the DEGs and uncovered that the early stage, which is from 8 to 17 days post-anthesis (DPA) plays an important role in black pigment biosynthesis and accumulation. The gene expression patterns were consistent with the seed color change. Besides, we studied the shared DEGs between the black and white sesame. We figured out 17 candidate genes associated with pigments biosynthesis in black sesame seed including 2 chalcone synthase genes SIN_1018961 and SIN_1018959 which may function in the phenylpropanoid pathway. 5 of these candidate genes, SIN_1006242 and SIN_1016759/PPO, SIN_1026689 and SIN_1006025, SIN_1025056 are located on chromosomes 4, 8 and 11 respectively, in conformity with previous QTL mapping. These genes were believed to play a major role in black seed development in sesame. Conclusion: This work illuminated the different expression profiles in black and white sesames and unfolded pivotal stages and a catalog of candidate genes associated with black seed formation in sesame. These findings provide a vast transcriptome dataset and list of genes that will be targeted for functional studies related to the molecular mechanism involved in biosynthesis and regulation of seed coat color in sesame and for molecular breeding of high-quality sesame varieties.

Analysis of QTLs for the micromorphology on the seed coat surface of soybean using recombinant inbred lines

2015 ◽  
Vol 25 (4) ◽  
pp. 409-415 ◽  
Author(s):  
Kazunori Otobe ◽  
Satoshi Watanabe ◽  
Kyuya Harada
Keyword(s):  
Seed Coat ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Soybean Seed ◽  
Coat Colour ◽  
Inbred Lines ◽  
Phenotypic Variance ◽  
Seed Coat Colour ◽  
Impermeable Layer ◽  
Phenotype Markers

AbstractThe seed coat of soybean (Glycine max (L.) Merrill) must protect the seed but allow water intake. Overprotection, causing impermeability, is assumed to be due to the presence of an impermeable layer in the seed coat, although validation of this assumption has relied on imbibition testing, which tends to be influenced by microfractures in the seed coat. Recent micromorphological analyses using laser-assisted topography microscopy revealed links to the surface roughness (SR) of the seed coat. To verify genetic links between hardseededness and SR, we analysed quantitative trait loci (QTLs) governing SR formation using 148 recombinant inbred lines (RILs) with a genetic linkage map covering 2663.6 cM of all 20 linkage groups of soybean, with 355 DNA markers and 5 phenotype markers. Five QTLs were detected, including previously identified hardseededness QTLs for ratio of seeds absorbing water, namely RAS1 and RAS2, which accounted for 20% of the phenotypic variance, and one near a locus inhibiting seed coat colour (I). These results indicate that the impermeability of soybean seed is genetically related to the reduction of SR.

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