scholarly journals Transcriptome Profile Analysis of Arabidopsis Reveals the Drought Stress-Induced Long Non-coding RNAs Associated With Photosynthesis, Chlorophyll Synthesis, Fatty Acid Synthesis and Degradation

2021 ◽  
Vol 12 ◽  
Author(s):  
Kang Chen ◽  
Yang Huang ◽  
Chunni Liu ◽  
Yu Liang ◽  
Maoteng Li
Keyword(s):  
Fatty Acid ◽  
Drought Stress ◽  
Fatty Acid Synthesis ◽  
Oil Content ◽  
Acid Synthesis ◽  
Chlorophyll Synthesis ◽  
Short Term ◽  
Non Coding Rnas ◽  
Mutant Lines

Long non-coding RNAs (lncRNAs) play an important role in the response of plants to drought stress. The previous studies have reported that overexpression of LEA3 and VOC could enhance drought tolerance and improve the oil content in Brassica napus and Arabidopsis thaliana, and most of the efforts have been invested in the gene function analysis, there is little understanding of how genes that involved in these important pathways are regulated. In the present study, the transcriptomic results of LEA3 and VOC over-expressed (OE) lines were compared with the RNAi lines, mutant lines and control lines under long-term and short-term drought treatment, a series of differentially expressed lncRNAs were identified, and their regulation patterns in mRNA were also investigated in above mentioned materials. The regulation of the target genes of differentially expressed lncRNAs on plant biological functions was studied. It was revealed that the mutant lines had less drought-response related lncRNAs than that of the OE lines. Functional analysis demonstrated that multiple genes were involved in the carbon-fixing and chlorophyll metabolism, such as CDR1, CHLM, and CH1, were regulated by the upregulated lncRNA in OE lines. In LEA-OE, AT4G13180 that promotes the fatty acid synthesis was regulated by five lncRNAs that were upregulated under both long-term and short-term drought treatments. The key genes, including of SHM1, GOX2, and GS2, in the methylglyoxal synthesis pathway were all regulated by a number of down-regulated lncRNAs in OE lines, thereby reducing the content of such harmful compounds produced under stress in plants. This study identified a series of lncRNAs related to the pathways that affect photosynthesis, chlorophyll synthesis, fatty acid synthesis, degradation, and other important effects on drought resistance and oil content. The present study provided a series of lncRNAs for further improvement of crop varieties, especially drought resistant and oil content traits.

scholarly journals Medium-chain fatty acids as short-term regulators of hepatic lipogenesis

1994 ◽  
Vol 302 (1) ◽  
pp. 141-146 ◽  
Author(s):  
M J H Geelen
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Acetyl Coa Carboxylase ◽  
Short Term ◽  
Acetyl Coa ◽  
Carboxylase Activity ◽  
Malonyl Coa ◽  
Stimulation Of

Short-term exposure of isolated rat hepatocytes to short- and medium-chain fatty acids led to an activation of acetyl-CoA carboxylase as measured in digitonin-permeabilized hepatocytes. Up to a certain concentration, typical for each of the fatty acids used, fatty acid-dependent activation of acetyl-CoA carboxylase coincided with an increase in the rate of fatty acid synthesis in intact hepatocytes, as determined by the incorporation of 3H from 3H2O water into fatty acids. At higher concentrations loss of stimulation of fatty acid synthesis occurred, but not the enhancement of carboxylase activity. With the fatty acids tested (C8:0-C14:0), the peak in fatty acid synthesis coincided with a peak in the level of malonyl-CoA. The onset of the stimulation of carboxylase activity coincided with the start of the peak in both fatty acid synthesis and malonyl-CoA. The longer the chain length of the fatty acid added, the lower the concentration at which the rate of fatty acid synthesis and the level of malonyl-CoA reached a peak and carboxylase activity started to become elevated. In cell suspensions incubated with increasing concentrations of fatty acids, accumulation of lactate decreased progressively. The latter observation, in combination with the fact that the activity of acetyl-CoA carboxylase is not always related to the rate of fatty acid biosynthesis, suggests that under these conditions not the activity of the carboxylase but the flux through the glycolytic sequence determines, at least in part, the rate of fatty acid synthesis de novo.

scholarly journals Prenatal Rosiglitazone Administration to Neonatal Rat Pups Does Not Alter the Adult Metabolic Phenotype

PPAR Research ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Hernan Sierra ◽  
Reiko Sakurai ◽  
W. N. Paul Lee ◽  
Nghia C. Truong ◽  
John S. Torday ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Troponin I ◽  
De Novo ◽  
Acid Synthesis ◽  
Neonatal Rat ◽  
Metabolic Phenotype ◽  
Fat Tissue ◽  
Lung Maturation

Prenatally administered rosiglitazone (RGZ) is effective in enhancing lung maturity; however, its long-term safety remains unknown. This study aimed to determine the effects of prenatally administered RGZ on the metabolic phenotype of adult rats.Methods. Pregnant Sprague-Dawley rat dams were administered either placebo or RGZ at embryonic days 18 and 19. Between 12 and 20 weeks of age, the rats underwent glucose and insulin tolerance tests andde novofatty acid synthesis assays. The lungs, liver, skeletal muscle, and fat tissue were processed by Western hybridization for peroxisome proliferator-activated receptor (PPAR)γ, adipose differentiation-related protein (ADRP), and surfactant proteins B (SPB) and C (SPC). Plasma was assayed for triglycerides, cholesterol, insulin, glucagon, and troponin-I levels. Lungs were also morphometrically analyzed.Results. Insulin and glucose challenges,de novofatty acid synthesis, and all serum assays revealed no differences among all groups. Western hybridization for PPARγ, ADRP, SPB, and SPC in lung, liver, muscle, and fat tissue showed equal levels. Histologic analyses showed a similar number of alveoli and septal thickness in all experimental groups.Conclusions. When administered prenatally, RGZ does not affect long-term fetal programming and may be safe for enhancing fetal lung maturation.

scholarly journals Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bohan Ma ◽  
Aijing Zhang ◽  
Qiuzhu Zhao ◽  
Zeyuan Li ◽  
Abraham Lamboro ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Fatty Acid Biosynthesis ◽  
Expression Patterns ◽  
Lipid Synthesis ◽  
Acid Synthesis ◽  
Differentially Expressed ◽  
Fatty Acid Transport ◽  
Protein Coding ◽  
Non Coding Rnas

AbstractLong non-coding RNAs (lncRNAs) are non-coding RNAs of more than 200 nucleotides. To date, the roles of lncRNAs in soybean fatty acid synthesis have not been fully studied. Here, the low-linolenic acid mutant ‘MT72′ and the wild-type control ‘JN18′ were used as materials. The lncRNAs in young pods at 30 and 40 days (d) after flowering were systematically identified and analyzed using transcriptome sequencing technology combined with bioinformatics tools. A total of 39,324 lncRNAs and 561 differentially expressed lncRNAs were identified. A lncRNAs-miRNAs-protein-coding genes (mRNAs) network was constructed, and 46 lncRNAs, 46 miRNAs and 137 mRNAs were found to be correlated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of 12 targeted mRNAs in the competing endogenous RNA network showed that these lncRNAs may be involved in the biological processes of fatty acid transport, lipid synthesis and cell division. Finally, the expression levels of differentially expressed lncRNAs, miRNAs and mRNAs were verified using qRT-PCR. The expression patterns of most genes were consistent with the sequencing results. In conclusion, new information was provided for the study of fatty acid synthesis by lncRNAs in young soybean pods.

scholarly journals Transcriptional Profiles of Long Non-coding RNAs Involved in Fatty Acid Biosynthesis at Filling Stage of Soybean Pods

2021 ◽  
Author(s):  
Bohan Ma ◽  
Yue Li ◽  
Mohamed Khalifa ◽  
Meng Teng ◽  
Aijing Zhang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Fatty Acid Biosynthesis ◽  
Acid Synthesis ◽  
Published Data ◽  
Acid Biosynthesis ◽  
Non Coding Rnas ◽  
Low Linolenic

Abstract Background: Long non-coding RNAs (lncRNAs) are non-coding RNAs of more than 200 nucleotides. To date, the roles of lncRNAs in soybean fatty acid synthesis have not been fully studied. Here, the low-linolenic acid ‘mutant 72’ (MT72) and the wild-type control ‘JiNong 18’(JN18) were used. Based on the previously published data on lncRNAs related RNA-seq in young pods of soybean 30 d to 40 d after flowering, lncRNAs and mRNAs from soybean pods 50 d after flowering were identified using high-throughput sequencing. The possible target genes of lncRNAs were predicted, and the functions related to fatty acid synthesis were analyzed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).Results: A total of 10,500 lncRNAs and 54,370 mRNAs were identified, and 115 possible target genes of 1,805 differentially expressed lncRNAs were found to be involved in fatty acid synthesis. A network of lncRNAs and mRNAs was constructed, and a total of 604 lncRNAs and 1,484 mRNAs had regulatory relationships. Among them, 115 target genes of 77 lncRNAs were directly or indirectly involved in fatty acid biosynthesis.Conclusions: The function related to fatty acid synthesis was predicted by differential expression of the target gene mRNAs interacting with lncRNAs. In conclusion, our results provide a theoretical basis for studies on fatty acid synthesis of lncRNAs in soybean.

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