Deployment of Brassica carinata A. Braun Derived Brassica juncea (L.) Czern. Lines for Improving Heterosis and Water Use Efficiency Under Water Deficit Stress Conditions

Among Brassica species, Ethiopian mustard (Brassica carinata A. Braun) is known to tolerate most abiotic stresses, including drought. Drought caused by low and erratic rainfall in semi-arid regions consistently challenges rapeseed mustard productivity. Development of B. carinata-derived lines (CDLs) in Brassica juncea (L.) Czern. nuclear background, carrying genomic segments from B. carinata, are expected to tolerate moisture deficit stress conditions. The present study was, thus, aimed to establish the phenomenon “heterosis” for drought tolerance and water use efficiency by evaluating 105 hybrids developed from intermating 15 CDLs in half diallel fashion. Data on 17 seed yield and yield contributing traits were recorded under two different environments, viz., irrigated and rainfed conditions. Traits under study were found to be governed by both additive and non-additive types of gene action. Average degree of dominance was higher (>2) for yield and yield contributing traits, viz., secondary branches/plant, point to first siliqua on main shoot, total siliquae/plant, 1,000-seed weight, seed yield/plant, biological yield, harvest index, and seed yield/hectare under rainfed conditions, clearly indicating that higher productivity under drought conditions can be realised through the development of hybrids. Out of 15, highly significant general combining ability (GCA) effects for seven CDLs were observed under rainfed condition. Furthermore, nine and six hybrids expressed highly significant specific combining ability (SCA) effects and > 50% heterobeltiosis for yield contributing traits under rainfed and irrigated conditions, respectively. Water use efficiency (WUE) of parental CDLs and hybrids varied from 2.05 to 2.57 kg m–3 under rainfed, while 1.10 to 1.28 kg m–3 under irrigated conditions. Hybrids expressed higher WUE than parental lines under both water regimes. Furthermore, selection indices such as drought tolerance index (DTI) and mean relative performance (MRP) were identified to be efficient in the selection of productive CDLs and hybrids under drought conditions. Nine hybrids, identified as highly productive in the present study, can further be exploited for improving the yield of Indian mustard in drought-prone areas. Usefulness of interspecific hybridisation in the development of B. carinata-derived B. juncea lines for improving heterosis and WUE is, thus, well demonstrated through the present study.

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Irrigation, nitrogen and sulphur fertilization response on productivity, water use efficiency and quality of Ethiopian mustard (Brassica carinata) in a semi-arid environment

Journal of Applied and Natural Science ◽

10.31018/jans.v10i2.1741 ◽

2018 ◽

Vol 10 (2) ◽

pp. 593-600

Author(s):

O.P. Verma ◽

S. Singh ◽

S. Pradhan ◽

G. Kar ◽

S.K. Rautaray

Keyword(s):

Water Use Efficiency ◽

Water Use ◽

Seed Yield ◽

Agricultural Research ◽

Arid Environment ◽

Brassica Carinata ◽

Ethiopian Mustard ◽

Use Efficiency ◽

Semi Arid

Water and nutrient deficiency are two major constraints that drastically affect rapeseed and mustard production under semi-arid regions of North-Western India. To cope with this problem, a study was undertaken to optimize irrigation, nitrogen and sulphur levels for productivity and quality of Ethiopian mustard(Brassica carinata).Field experiments were conducted during winter seasons of 2004-05 and 2005-06 at the Water Technology Centre (WTC), Indian Council of Agricultural Research (ICAR) – Indian Agricultural Research Institute (IARI), New Delhi, India. Irrigation, nitrogen and sulphur levels significantly (P<0.05) affected plant height, LAI, siliqua weight, seeds/siliqua, test weight, seed and biomass yield of Ethiopian mustard. Application of three irrigations (seedling, 50% flowering and pod development stage) to Ethiopian mustard increased seed yield by 27-28% compared to one irrigation (seedling stage). Nitrogen @ 90 kg ha-1 produced 49-54 % higher seed yield of Ethiopian mustard compared to no application. Similarly, sulphur application @ 40 kg ha-1 increased seed yield by 33-34 % compared to no application of sulphur. The oil content of Ethiopian mustard was significantly (P<0.05) affected by nitrogen and sulphur levels. Among the three treatments, irrigation treatment only significantly affected evapotranspiration of the studied crop. From the above study, it is suggested that application of 3 irrigations with 90 kg N ha-1 and 40 kg S ha-1 may be practiced for achieving higher seed yield, quality and water use efficiency of Ethiopian mustard in the semi-arid environment of Northern part of India.

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Overexpression of MdATG8i improves water use efficiency in transgenic apple by modulating photosynthesis, osmotic balance, and autophagic activity under moderate water deficit

Horticulture Research ◽

10.1038/s41438-021-00521-2 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Xin Jia ◽

Ke Mao ◽

Ping Wang ◽

Yu Wang ◽

Xumei Jia ◽

...

Keyword(s):

Water Use Efficiency ◽

Water Deficit ◽

Water Use ◽

Photosynthetic Capacity ◽

Critical Role ◽

Drought Conditions ◽

Moderate Drought ◽

Use Efficiency ◽

Autophagic Activity

AbstractWater deficit is one of the major limiting factors for apple (Malus domestica) production on the Loess Plateau, a major apple cultivation area in China. The identification of genes related to the regulation of water use efficiency (WUE) is a crucial aspect of crop breeding programs. As a conserved degradation and recycling mechanism in eukaryotes, autophagy has been reported to participate in various stress responses. However, the relationship between autophagy and WUE regulation has not been explored. We have shown that a crucial autophagy protein in apple, MdATG8i, plays a role in improving salt tolerance. Here, we explored its biological function in response to long-term moderate drought stress. The results showed that MdATG8i-overexpressing (MdATG8i-OE) apple plants exhibited higher WUE than wild-type (WT) plants under long-term moderate drought conditions. Plant WUE can be increased by improving photosynthetic efficiency. Osmoregulation plays a critical role in plant stress resistance and adaptation. Under long-term drought conditions, the photosynthetic capacity and accumulation of sugar and amino acids were higher in MdATG8i-OE plants than in WT plants. The increased photosynthetic capacity in the OE plants could be attributed to their ability to maintain optimal stomatal aperture, organized chloroplasts, and strong antioxidant activity. MdATG8i overexpression also promoted autophagic activity, which was likely related to the changes described above. In summary, our results demonstrate that MdATG8i-OE apple lines exhibited higher WUE than WT under long-term moderate drought conditions because they maintained robust photosynthesis, effective osmotic adjustment processes, and strong autophagic activity.

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Effects of Irrigation and Nitrogen Fertilization on Seed Yield, Yield Components, and Water Use Efficiency of Cleistogenes songorica

Agronomy ◽

10.3390/agronomy11030466 ◽

2021 ◽

Vol 11 (3) ◽

pp. 466

Author(s):

Qibo Tao ◽

Mengjie Bai ◽

Cunzhi Jia ◽

Yunhua Han ◽

Yanrong Wang

Keyword(s):

Water Use Efficiency ◽

Water Use ◽

Seed Yield ◽

Yield Components ◽

Perennial Grass ◽

Yield Component ◽

Path Coefficient ◽

Grass Seed ◽

Significant Difference ◽

Use Efficiency

Irrigation and nitrogen (N) are two crucial factors affecting perennial grass seed production. To investigate the effects of irrigation and N rate on seed yield (SY), yield components, and water use efficiency (WUE) of Cleistogenes songorica (Roshevitz) Ohwi, an ecologically significant perennial grass, a four-year (2016–2019) field trial was conducted in an arid region of northwestern China. Two irrigation regimes (I1 treatment: irrigation at tillering stage; I2 treatment: irrigation at tillering, spikelet initiation, and early flowering stages) and four N rates (0, 60, 120, 180 kg ha−1) were arranged. Increasing amounts of both irrigation and N improved SY, evapotranspiration, WUE, and related yield components like fertile tillers m−2 (FTSM) and seeds spikelet−1. Meanwhile, no significant difference was observed between 120 and 180 kg N ha−1 treatments for most variables. The highest SY and WUE was obtained with treatment combination of I2 plus 120 kg N ha−1 with four-year average values of 507.3 kg ha−1 and 1.8 kg ha−1 mm−1, respectively. Path coefficient and contribution analysis indicated that FTSM was the most important yield component for SY, with direct path coefficient and contribution coefficient of 0.626 and 0.592. Overall, we recommend I2 treatment (three irrigations) together with 120 kg N ha−1 to both increase SY and WUE, especially in arid regions. Future agronomic managements and breeding programs for seed should mainly focus on FTSM. This study will enable grass seed producers, plant breeders, and government program directors to more effectively target higher SY of C. songorica.

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