scholarly journals Comparison of Multi-Gene Testing Data Between Fresh and Formalin-Fixed Specimens From Core Needle Biopsy in Patients With NSCLC

2021 ◽  
Vol 27 ◽  
Author(s):  
Zhi-Gang Yao ◽  
Zhi-Gang Wei ◽  
Xian-Kui Cheng ◽  
Guang-Hui Huang ◽  
Yuan-Yuan Zong ◽  
...  
Keyword(s):  
Core Needle Biopsy ◽  
Needle Biopsy ◽  
Testing Procedure ◽  
Core Needle ◽  
Gene Testing ◽  
Cell Counts ◽  
Significant Difference ◽  
Ffpe Tissues ◽  
Nsclc Patients ◽  
Formalin Fixed

Purpose: Currently, formalin-fixed paraffin-embedded (FFPE) tissue specimens are the conventional material for gene testing for non-small cell lung cancer (NSCLC) patients. In our study, we aimed to develop a quick gene testing procedure using fresh core needle biopsy samples from NSCLC patients.Methods: In total, 77 fresh NSCLC samples obtained from core needle biopsy were evaluated by frozen section examination. If the NSCLC diagnosis and adequate tumor cell counts were confirmed by histopathology, the fresh tissues were used to extract DNA and subsequent gene testing by ARMS-PCR. Meanwhile, the paired FFPE core needle biopsy samples from 30 NSCLC patients also underwent gene testing.Results: In total, 77 fresh samples showed an EGFR mutation rate of 61.0%, higher than the levels in the Asian. Following a comparison of gene testing results with fresh tissues and paired FFPE tissues from the 30 patients, no significant difference in the DNA concentration extracted from fresh tissues and FFPE tissues was found. However, DNA purity was significantly higher in fresh tissues than that in FFPE tissues. Gene testing detected the same gene mutations in 93.3% of cases in fresh tissues and paired FFPE tissues. The gene testing procedure using fresh biopsy samples greatly shortens the waiting time of patients.Conclusion: The multi-gene mutation testing using fresh core needle biopsy samples from NSCLC patients is a reasonable, achievable, and quick approach. Fresh tissues may serve as a potential alternative to FFPE tissues for gene testing in NSCLC patients.

The reliability of HER2-status determination from core-needle-biopsies and surgical specimens: A comparison of two established test methods (IHC, CISH)

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e22141-e22141
Author(s):  
V. Wolf ◽  
R. Groβe ◽  
J. Erggelet ◽  
H. J. Holzhausen ◽  
S. Hauptmann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Core Needle Biopsy ◽  
Needle Biopsy ◽  
In Situ Hybridisation ◽  
Test Method ◽  
Her2 Status ◽  
Surgical Specimen ◽  
Core Needle ◽  
Significant Difference ◽  
Her 2

e22141 Background: A milestone of breast-cancer therapy was the discovery of HER-2 entailing special targeted therapy with improved prognosis. The HER-2-status is routinely assessed through immunohistochemistry (IHC; HercepTest) showing protein over-expression and is double-checked with in-situ-hybridisation (ISH) demonstrating gene amplification in equivocal cases. It is questioned whether these methods achieve identical results in core-needle-biopsies and in excisional tumor specimens. Methods: We performed a retrospective comparative study in order to address these questions. From 01/03–06/08 we collected the HercepTest results from both core-needle-biopsy and surgical specimen of 109 breast cancer patients in our institute and compared these to newly evaluated chromogenic ISH (CISH) results for both specimen types in order to assess the reliability of HER-2- diagnosis of both methodological approaches and of specimen type. Results: We found no significant difference in the HER- 2-status determined from either needle-biopsies or surgical specimens irrespective of the test used. For the overall comparison (218 specimens) of HercepTest and CISH we found only slight, non-significant deviations. Four cases were CISH-negative in spite of HercepTest scoring of 3+. Vice versa, five out of the total of 38 (17.4%) CISH-positives did not correspond to the HercepTest results of 0 or 1+. Conclusions: Though not significant, there is some inconsistency in the HER-2-determination depending on the test-method, leaving these cases equivocal. In accordance with the literature, we therefore recommend to at least double-check samples with 2+ in the HercepTest as it is the current standard. Our data support the use of core-needle-biopsy as a reliable tissue sample for HER-2-diagnosis. [Table: see text]

Value of biomarkers in distinguishing indeterminate core needle biopsy samples of thyroid nodules

2020 ◽  
Author(s):  
Yan Xiong ◽  
Li Liang ◽  
Limin Yan ◽  
Dong Li ◽  
Xin Li ◽  
...  
Keyword(s):  
Core Needle Biopsy ◽  
Predictive Value ◽  
Needle Biopsy ◽  
Thyroid Nodules ◽  
Fine Needle Biopsy ◽  
Pathogenic Mutation ◽  
Core Needle ◽  
Gene Testing ◽  
Galectin 3 ◽  
Sensitivity Specificity

Abstract Background: Core needle biopsy (CNB) is now more frequently used for the preoperative diagnosis of thyroid nodules. Based on morphology alone, 5%-20% of CNB samples cannot be determined as malignant or benign. Compared to fine-needle biopsy (FNB), samples collected by CNB are more accessible for various tests. Therefore, studying the application of biomarkers in distinguishing indeterminate CNB samples of thyroid nodules is a practical need. Methods: Patients with thyroid nodules with both CNB and matched resected specimens were reviewed. Cases classified as indeterminate lesions, follicular neoplasms and suspicious for malignancy were retrieved. All CNB samples were stained by immunohistochemistry (IHC) using antibodies against CK19, Galectin-3, HBME-1, and CD56 and detected by next-generation sequencing (NGS) using a target panel. With the help of these biomarkers, all CNB samples were reclassified. Taking the classification of resected specimens as the gold standard, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of each biomarker for discriminating malignancy from benignity were calculated. Results: The sensitivity, specificity, PPV, NPV and accuracy were 93.55%, 60.00%, 93.55%, 60.00% and 88.89% for CK19; 93.55%, 40.00%, 90.63%, 50.00% and 86.11% for Galectin-3; 77.42%, 100.00%, 100.00%, 41.67% and 80.56% for HBME-1; 66.13%, 100.00%, 100.00%, 32.26% and 70.83% for CD56; and 91.94%, 100.00%, 100.00%, 66.67% and 93.06% for pathogenic mutation. Conclusions: The application of biomarkers is very effective in distinguishing indeterminate CNB samples of thyroid nodules. Gene testing by NGS using a target panel has very high accuracy. The limitation of tumor quantity is the main reason for the weakened power of NGS. IHC plays an important role in cases with negative NGS results. The combination of NGS and IHC is a reliable “rule in” test for malignancy.

scholarly journals Relative Sensitivity of Core-Needle Biopsy and Incisional Biopsy in the Diagnosis of Musculoskeletal Sarcomas

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1393
Author(s):  
Alexander Klein ◽  
Theresa Fell ◽  
Christof Birkenmaier ◽  
Julian Fromm ◽  
Volkmar Jansson ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Core Needle Biopsy ◽  
Needle Biopsy ◽  
Histopathological Examination ◽  
Statistical Significance ◽  
Soft Tissue Sarcomas ◽  
Incisional Biopsy ◽  
Tissue Samples ◽  
Core Needle ◽  
Significant Difference

Background: There is no evidence as to the diagnostic value of the two most frequently used methods of biopsies in sarcomas: Incisional or core needle biopsy. The aim of our study was to evaluate the diagnostic sensitivity of the incisional and the core needle biopsy techniques in the diagnosis of bone and soft tissue sarcomas. Methods: We included 417 patients with a definitive diagnosis of bone or soft tissue sarcoma in whom a total of 472 biopsies had been performed. We correlated the results of the biopsies with the result of the definitive histopathological examination of the resected tumor. Dignity, entity, and grading (whenever possible) of the tissue samples were evaluated. Results: A total of 258 biopsies (55%) were performed in order to diagnose a soft tissue tumor and 351 biopsies (74.4%) were core needle biopsies. The number of repeat core needle biopsies, necessitated because of inconclusive histopathological results, was significantly higher (50 vs. 5; p = 0.003). We observed no significant difference regarding dignity, entity, and grading between the 2 different types of biopsies. Only with regards to the determination of dignity and entity of chondroid tumors, incisional biopsy was superior with statistical significance (p = 0.024). Conclusions: This study represents the largest study on biopsies for bone and soft tissue sarcomas. Based only on our results, we are unable to favor one method of biopsy and found high accuracy with both methods. Considering the potential complications, the added oncological risks of incisional biopsies and the ready availability of core needle biopsies, the latter, in our assessment, represents a valid and favourable method for bone and soft tissue sarcomas.

scholarly journals Comparison of Usefulness of Ultrasound-Guided Fine Needle Aspiration and Core Needle Biopsy in Diagnosis of Cervical Lymphadenopathy

2021 ◽  
Vol 64 (8) ◽  
pp. 568-575
Author(s):  
Ju Yong Kang ◽  
Jung Jun Kim ◽  
Brian Kim ◽  
Myung-Chul Lee ◽  
Ik Joon Choi
Keyword(s):  
Malignant Lymphoma ◽  
Fine Needle Aspiration ◽  
Core Needle Biopsy ◽  
Predictive Value ◽  
Needle Biopsy ◽  
Cervical Lymphadenopathy ◽  
Needle Aspiration ◽  
Core Needle ◽  
Fine Needle ◽  
Significant Difference

Background and Objectives The purpose of this study is to compare diagnostic outcome and safety of fine needle aspiration (FNA) and core needle biopsy (CNB) in patients with cervical lymphadenopathy. Subjects and Method This retrospective studies were conducted on 164 cases with cervical lymphadenopathy that had undergone FNA, and 44 cases that had undergone CNB. The cytopathological results from FNA and CNB were compared with the histopathological results confirmed by excisional biopsy in the nodes according to different sizes and radiological levels, respectively. Diagnostic sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were evaluated according to disease entities.Results FNA is more accurate than CNB in most of the sizes and at all cervical levels. However, there is no statistically significant difference between the results of the two tests. Sensitivity and NPV for diagnosis of malignant lymphoma are higher with FNA than with CNB. However, specificity and PPV for malignant lymphoma are higher with CNB than with FNA. Also CNB showed superior sensitivity, PPV and NPV for metastatic lymphadenopathy. Conclusion Although CNB is beneficial in reducing the false negative rate, it still has several limitations. Since CNB has no statistically significant advantage over FNA, FNA can be a good alternative method with less complication and high diagnostic value for lesions where CNB is difficult to perform.

Effect of core needle biopsy number on intraductal carcinoma diagnosis in patients with metastatic castration-sensitive prostate cancer.

2020 ◽  
Vol 38 (6_suppl) ◽  
pp. 243-243
Author(s):  
Masashi Kato ◽  
Toyonori Tsuzuki ◽  
Akiyuki Yamamoto ◽  
Takashi Fujita ◽  
Momokazu Gotoh
Keyword(s):  
Prostate Cancer ◽  
Prognostic Marker ◽  
Core Needle Biopsy ◽  
Needle Biopsy ◽  
Metastatic Prostate Cancer ◽  
The Other ◽  
Intraductal Carcinoma ◽  
P Value ◽  
Core Needle ◽  
Significant Difference

243 Background: The number of core needle biopsies performed to diagnose metastatic prostate cancer are sometimes reduced in daily clinical practice. Intraductal carcinoma of the prostate (IDC-P) is associated with adverse prognostic parameters and has recently received attention as a valuable indicator for the prediction of disease severity. Currently, the relationship between IDC-P diagnosis and the number of core prostate biopsies is unclear. In the present study, we analyzed the effect of core needle biopsy number on IDC-P diagnosis in patients with metastatic castration-sensitive prostate cancer. Methods: We retrospectively evaluated data from 150 patients diagnosed with metastatic prostate cancer at our hospital between 2002 and 2012. Percentage of the core involved with cancer and the maximum cancer occupancy were 100% in median of all the patients. Subjects were allocated to three groups according to the number of core biopsies performed: ≤5, 6–9, and ≥10. The study endpoints were the cancer-specific survival (CSS) and overall survival (OS) rates. Results: Twenty-seven (18%) patients had ≤5 core biopsies, 67 (45%) patients had 6–9 core biopsies, and 56 (37%) patients had ≥10 core biopsies. For patients who underwent ≥10 core biopsies, a significant difference on CSS was detected between with or without IDC-P ( p = 0.002). On the other hand, the difference decreased as the number of core biopsies became smaller (6–9; p = 0.322 and ≤5; p = 0.815). A similar trend was identified for the OS outcome. A significant difference on OS was also found between with or without IDC-P in patients who underwent ≥10 and 6–9 core needle biopsies ( p = 0.0002 and 0.015, respectively), but not in those who underwent ≤5 core biopsies ( p = 0.3407). IDC-P served as a stronger prognostic marker for CSS and OS than did the other factors included in the multivariate analysis. ( p = 0.0024, and p = 0.0014, respectively). Conclusions: Given the IDC-P detection and its value as a prognostic marker, we recommend the performance of ≥10 core biopsy procedures in patients diagnosed with metastatic prostate cancer. Sampling error effects the IDC-P value in smaller number of core needle biopsies.

Core needle biopsy wash as a tool for acquiring additional diagnostic material for laboratory testing

2021 ◽  
pp. jclinpath-2020-207318
Author(s):  
Wilfrido Mojica ◽  
Katherine Cwiklinski ◽  
Xiaobing Jin ◽  
Weiguo Liu ◽  
Donald Yergeau
Keyword(s):  
Core Needle Biopsy ◽  
Needle Biopsy ◽  
Laboratory Testing ◽  
Common Procedure ◽  
Core Needle ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Tissue Procurement ◽  
Diagnostic Capabilities ◽  
Formalin Fixed

AimsTo determine if a simple prewash step added to the processing workflow of tissue procurement by a core needle biopsy device will recover enough cells to expand the laboratory testing armamentarium.MethodsTissue was obtained from unfixed resection specimens using a core needle device and washed in a buffered solution before fixation. This creates a liquid aliquot from which dislodged cells can be kept and separated from the tissue specimen, the latter of which can then undergo traditional formalin-fixed, paraffin-embedded processing.ResultsCells dislodged from the tissue during the biopsy procedure are recoverable, are representative of the tissue section and of sufficient quantities for additional laboratory testing.ConclusionsThe core needle biopsy wash is an under-recognised and underutilised approach to extending the diagnostic capabilities of the limited amount of targeted material obtained during this common procedure. The ability to recover supplemental amounts of diagnostic material yields great potential as a substrate for a multitude of current and developing laboratory assays.

scholarly journals Evaluation of Ki-67 Index in Core Needle Biopsies and Matched Breast Cancer Surgical Specimens

2017 ◽  
Vol 142 (3) ◽  
pp. 364-368 ◽  
Author(s):  
Soomin Ahn ◽  
Junghye Lee ◽  
Min-Sun Cho ◽  
Sanghui Park ◽  
Sun Hee Sung
Keyword(s):  
Breast Cancer ◽  
Core Needle Biopsy ◽  
Needle Biopsy ◽  
Absolute Difference ◽  
Image Analyzer ◽  
Luminal A ◽  
Ki 67 ◽  
Surgical Specimen ◽  
Core Needle ◽  
Significant Difference

Context.— The Ki-67 index is strongly prognostic and is used as a surrogate marker to distinguish luminal A from luminal B breast cancer types. Objective.— To investigate differences in Ki-67 index between core needle biopsy samples and matched surgical samples in breast cancer. Design.— We included patients with invasive breast cancer who did not receive neoadjuvant therapy. A total of 89 pairs of core needle biopsies and surgical specimens were collected, and the Ki-67 index was assessed in hot spot areas using an image analyzer. We applied a 14% Ki-67 index to define low versus high groups. Results.— The Ki-67 index was significantly higher in core needle biopsies than in surgical specimens (P < .001), with a median absolute difference of 3.5%. When we applied 14% as a cutoff, 16 of 89 cases (18%) showed discrepancy. Thirteen cases showed a high Ki-67 index in core needle biopsies but a low Ki-67 index in surgical samples. There were 10 cases (11.2%) that showed discordant luminal A/B types between core needle biopsy and the matched surgical specimen. The reasons for the discordance were poor staining of MIB1 accompanied by fixation issues and intratumoral heterogeneity of the Ki-67 index. Conclusions.— A significant difference in the Ki-67 index between core biopsy and surgical specimens was observed. Our findings indicate that it may be better to perform the Ki-67 assay on the core needle biopsy and the surgical specimen than on only one sample.

Percutaneous Imaging-Guided versus Open Musculoskeletal Biopsy: Concepts and Controversies

2020 ◽  
Vol 24 (06) ◽  
pp. 667-675
Author(s):  
Violeta Vasilevska Nikodinovska ◽  
Slavcho Ivanoski ◽  
Milan Samardziski ◽  
Vesna Janevska
Keyword(s):  
Soft Tissue ◽  
Complication Rate ◽  
Core Needle Biopsy ◽  
Multidisciplinary Team ◽  
Gold Standard ◽  
Needle Biopsy ◽  
Soft Tissue Tumors ◽  
Open Biopsy ◽  
Core Needle ◽  
Similar Accuracy

AbstractBone and soft tissue tumors are a largely heterogeneous group of tumors. Biopsy of musculoskeletal (MSK) tumors is sometimes a challenging procedure. Although the open biopsy is still considered the gold standard for the biopsy of MSK lesions, core needle biopsy can replace it in most cases, with similar accuracy and a low complication rate. The biopsy should be performed in a tertiary sarcoma center where the multidisciplinary team consists of at minimum a tumor surgeon, an MSK pathologist, and an MSK radiologist who can assess all steps of the procedure. Several factors can influence the success of the biopsy including the lesion characteristics, the equipment, and the method used for the procedure. This review highlights some of the important aspects regarding the biopsy of the MSK tumors, with special attention to imaging a guided core needle biopsy and highlighting some of the recent advancements and controversies in the field.

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