Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia

Background and Aim: The practice of keeping animals as pets is becoming increasingly common. The upsurge of extended-spectrum β-lactamase (ESBL)-producing organisms of animal origin is a health threat globally. This study aimed to identify the presence of extended-spectrum β-lactamase-producing Escherichia coli in companion dogs in animal clinics in Surabaya, Indonesia. Materials and Methods: A total of 85 rectal swab samples were collected from companion dogs at five animal clinics in different regions of Surabaya, Indonesia. The presence of E. coli was identified from the samples using standard methods, followed by antibiotic sensitivity testing. The resistant isolates were examined for the presence of ESBL using the double-disk synergy test method. The phenotypically identified ESBL-producing E. coli was further confirmed with an automated system using Vitek-2. Results: The rectal swab samples (n=85) tested were 100% positive for E. coli isolates. Eight (9.41%) out of the 85 E. coli obtained from rectal swabs were extended-spectrum β-lactamase producers. All eight ESBL-producing E. coli were identified by automated Vitek-2 confirmatory tests. Conclusion: This study provides insight into the prevalence of ESBL-producing organisms isolated from companion dogs in Indonesia. This work indicates the need for the general public to be more aware of the role of companion animals in disseminating pathogenic organisms, since they serve as potential reservoirs in the spread of antibiotic resistance affecting human health.

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IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG

Journal of Basic Medical Veterinary ◽

10.20473/.v8i2.20414 ◽

2020 ◽

Vol 8 (2) ◽

pp. 108

Author(s):

Akyun Rozaqi Syah Putra ◽

Mustofa Helmi Effendi ◽

Setiawan Koesdarto ◽

Suwarno Suwarno ◽

Wiwik Tyasningsih ◽

...

Keyword(s):

Escherichia Coli ◽

Dairy Cow ◽

Pathogenic Bacteria ◽

Rectal Swab ◽

Test Method ◽

Coli Isolate ◽

E Coli ◽

Extended Spectrum ◽

Vitek 2 ◽

Synergy Test

Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the study showed that from all of the samples could be isolated and indentified E. coli, based on the colony characteristics on EMBA and biochemical test. Based on the double disc synergy test method using antibiotic disc amoxicylyn-clavulanate, ceftriaxone, aztreonam, ceftazidime and cefotaxime, 10 isolates could be identify els ESBL- E. coli. furthermore 3 out of 10 isolates DDST positives were confirmed ESBL- E. coli using Vitek-2 method.

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Evaluation of detection of extended-spectrum beta-lactamases among Escherichia coli and Klebsiella spp. isolates by VITEK 2 AST-N029 compared to the agar dilution and disk diffusion methods

Scandinavian Journal of Infectious Diseases ◽

10.1080/00365540701704706 ◽

2008 ◽

Vol 40 (5) ◽

pp. 355-362 ◽

Cited By ~ 7

Author(s):

Sofia D. Nyberg ◽

Olli Meurman ◽

Jari Jalava ◽

Kaisu Rantakokko-Jalava

Keyword(s):

Escherichia Coli ◽

Disk Diffusion ◽

Beta Lactamases ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases ◽

Agar Dilution ◽

Vitek 2 ◽

Klebsiella Spp

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Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-011-1169-2 ◽

2011 ◽

Vol 30 (7) ◽

pp. 869-872 ◽

Cited By ~ 2

Author(s):

G. Valenza ◽

S. Müller ◽

C. Schmitt ◽

D. Turnwald ◽

T-T. Lam ◽

...

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Klebsiella Oxytoca ◽

Beta Lactamases ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases ◽

Vitek 2

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The accuracy of extended-spectrum beta-lactamase detection in Escherichia coli and Klebsiella pneumoniae in South African laboratories using the Vitek 2 Gram-negative susceptibility card AST-N255

Southern African Journal of Infectious Diseases ◽

10.4102/sajid.v34i1.114 ◽

2019 ◽

Vol 34 (1) ◽

Cited By ~ 1

Author(s):

Andrea L. Young ◽

Mark P. Nicol ◽

Clinton Moodley ◽

Colleen M. Bamford

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

South African ◽

Reference Standard ◽

Gram Negative ◽

E Coli ◽

Extended Spectrum ◽

Vitek 2 ◽

Negative Susceptibility ◽

Composite Reference Standard

Background: Phenotypic detection of extended-spectrum beta-lactamases (ESBLs) is based on the inhibition of ESBL enzymes by β-lactamase inhibitors and on the comparison of cephalosporin activity with or without a β-lactamase inhibitor. Many South African diagnostic laboratories rely on the Vitek 2 for automated susceptibility testing and for ESBL detection. However, the Gram-negative susceptibility card currently used locally (AST-N255) has been modified and its accuracy for ESBL detection is not known.Methods: We randomly selected 50 isolates of Klebsiella pneumoniae and Escherichia coli from a collection of clinical bloodstream isolates from Groote Schuur Hospital from 2015 to 2016, including ESBL-producing and non-ESBL-producing strains. We used standardised phenotypic (disc diffusion and broth microdilution) and genotypic (conventional polymerase chain reaction (PCR) for blaCTX-M, blaSHV and blaTEM) methods for detection of ESBLs. We compared ESBL detection by Vitek 2 to a composite reference standard comprising ESBL detection either by both phenotypic methods or by one phenotypic method together with genotypic detection.Results: The sensitivity of Vitek 2 system for detection of ESBLs was 33/36 or 92% (78% – 97%) for E. coli, and 40/40 or 100% (91% – 100%) for K. pneumoniae, whilst specificity was 10/10 or 100% (72% – 100%) and 9/10 or 90% (60% – 98%), respectively. This is comparable with previous studies.Conclusion: Using a composite reference standard of the phenotypic and genotypic methods employed in this study, no Vitek-categorised ESBL E. coli or K. pneumoniae was found to be a non-ESBL with the exception of possible misinterpretation with K. pneumoniae SHV-hyper-producing isolates.

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