scholarly journals Application of Plant Extracts in Micropropagation and Cryopreservation of Bleeding Heart: An Ornamental-Medicinal Plant Species

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 542
Author(s):  
Dariusz Kulus ◽  
Natalia Miler
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Extracts ◽  
The Other ◽  
White Gold ◽  
Natural Extracts ◽  
Other Hand ◽  
The Impact

Lamprocapnos spectabilis (L.) Fukuhara (bleeding heart) is valued both in the horticultural and pharmaceutical markets. Despite its great popularity, information on the in vitro tissue culture technology in this species is limited. There is also little knowledge on the application of plant extracts in the tissue culture systems of plants other than orchids. The aim of this study is to compare the utility of traditional plant growth regulators (PGRs) and natural extracts—obtained from the coconut shreds, as well as oat, rice, and sesame seeds—in the micropropagation and cryopreservation of L. spectabilis ‘Gold Heart’ and ‘White Gold’. The biochemical analysis of extracts composition is also included. In the first experiment related to micropropagation via axillary buds activation, the single-node explants were cultured for a 10-week-long propagation cycle in the modified Murashige and Skoog medium fortified either with 1.11 µM benzyladenine (BA) and 1.23 µM indole-3-butritic acid (IBA) or with 10% (v/v) plant extracts. A PGRs- and extract-free control was also considered. In the cryopreservation experiment, the same 10% (v/v) extracts were added into the medium during a seven-day preculture in the encapsulation-vitrification cryopreservation protocol. It was found that the impact of natural additives was cultivar- and trait-specific. In the first experiment, the addition of coconut extract favoured the proliferation of shoots and propagation ratio in bleeding heart ‘Gold Heart’. Rice extract, on the other hand, promoted callus formation in ‘White Gold’ cultivar and was more effective in increasing the propagation ratio in this cultivar than the conventional plant growth regulators (4.1 and 2.6, respectively). Sesame extract suppressed the development of the explants in both cultivars analysed, probably due to the high content of polyphenols. As for the second experiment, the addition of plant extracts into the preculture medium did not increase the survival level of the cryopreserved shoot tips (sesame and oat extracts even decreased this parameter). On the other hand, coconut extract, abundant in simple sugars and endogenous cytokinins, stimulated a more intensive proliferation and growth of shoots after rewarming of samples. Analysing the synergistic effect of conventional plant growth regulators and natural extracts should be considered in future studies related to L. spectabilis.

scholarly journals Abscisin II and , some. Hormone-Regulated Plant Responses

1967 ◽  
Vol 20 (5) ◽  
pp. 869 ◽  
Author(s):  
D Aspinall ◽  
LG Paleg ◽  
FT Addicott
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Seedling Growth ◽  
Growth Regulators ◽  
The Other ◽  
Plant Responses ◽  
Acid Gas ◽  
Radicle Elongation ◽  
Other Hand

The aotivity of (�)-absoisin II' [(�)AbII] and its interaction with a number of plant growth regulators in the oontrol of lettuce germination, lettuoe hypoootyl and radiole elongation, ououmber seedling growth, radish lea ( senesoenoe, and barley vernalization were examined. The interac.tions between (� )AbII and th�;l promoting h~rmones were of two general types. In gibberellio acid (GAs)-promoted lettuoe germination and kinetin-oontrolled leaf senesoenoe, the effeots of low ooncentrations of ( � )-!\bII were oompletely overoome by high oonoentratioileof the other substanoe. In .the second type of interaction, (� )AbII was inhibitory only in the presenoe of high oonoentrations of the promoter (kinetin in lettuce germinatipn and allogibberio acid in lettuoe radicle elongation). Cuoumber radiole elongation, on the other hand, was promoted by (� )AbII in the presenoe of a mixture of GA4 and GA7.

scholarly journals Quantitative and Morphological Effect of Plant Growth Regulators on Callus Initiation from Leaves and Roots Explants of Artemisia annua L.

2020 ◽  
Vol 9 ◽  
pp. 11-17
Author(s):  
Wajeeha Iffat ◽  
Sohail Hussain
Keyword(s):  
Growth Hormone ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Artemisia Annua ◽  
The Other ◽  
Other Hand ◽  
The Media ◽  
Leaves And Roots ◽  
Artemisinin Content

To study the effect of some plant growth regulators on the in vitro initiation of callus using the leaves and roots explants of Artemisia annua. The experiment was arranged in a completely randomized design with 2 replicates. Morphologically leaf explants culture in media having the composition MS Basal + NAA (2.0mg/l) + BA(1.0 mg/l) shows best results as compared to all other media. However in roots shows no growth in all the media composition. On the other hand media having a composition (MS Basal + 2,4-D(1.0mg/l) + Kn (0.1mg/l)) with growth hormone antagonist shows no callus formation neither in leaf or roots. Our studies are also supported by the HPLC results about the artemisinin content in leaf in media having the composition MS Basal + NAA (2.0mg/l) + BA(1.0 mg/l) is 0.052% and least in media a composition (MS Basal + 2,4-D(1.0mg/l) + Kn (0.1mg/l)) have only 0.012%. On the other hand, we didn’t found any artemisinin content in the root explants in all the media. Our present study suggest that Benzyl adenine (BA) could be used as a promising growth hormone in tissue culture.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

Impact of Exogenous Plant Growth Regulators on Physiological Traits, Tendril and Flowering in Horsegram (Macrotyloma uniflorum Lam) under Kharif Season 

2021 ◽  
Author(s):  
R. Sivakumar
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Foliar Application ◽  
Leaf Temperature ◽  
Physiological Traits ◽  
Mepiquat Chloride ◽  
Kharif Season ◽  
Significant Difference ◽  
The Impact

Background: Horsegram is an important pulse crop grown as Rabi crop in Tamil Nadu. Kharif sown crop could not flowers may be because of more number of tendrils produced as compare to Rabi sown crop. Hence, an attempt was made to reduce the number of tendrils and induce flowering in horsegram during Kharif season by plant growth regulators. Methods: A field experiment was conducted to study the impact of plant growth regulators viz., salicylic acid (100 ppm), chlormequat chloride (CCC - 250 ppm), mepiquat chloride (250 ppm), tri iodo benzoic acid (TIBA - 200 ppm) and nitrobenzene (0.2%) on physiological traits and tendril growth associated with flowering in horsegram during Kharif. Different treatments were applied through foliar application at 25 Days after sowing. Result: Among the PGRs, foliar application of TIBA registered the lowest number of tendrils (2.3) followed by CCC (3.0) compared to other treatments. Higher root length of 16.8 cm and RWC of 82.3% was found in CCC treatment. CCC also registered the highest photosynthetic rate (27.15 µmol m-2 s-1), transpiration rate (18.06 mmol m-2 s-1) and lowest leaf temperature (26°C) compared to other treatments. The highest soluble protein content of 13.1 mg g-1 was also estimated in CCC treatment followed by mepiquat chloride (12.51 mg g-1). Sucrose phosphate synthase (SPS) activity did not show any significant difference between the treatments. Number of flowers buds formed per plant was zero and hence the flowering did not take place in any treatments. Among the plant growth regulators used, TIBA and CCC registered its positive action on reduced the number of tendrils and leaf temperature, but not enough to induce flowering under Kharif season.

Impact of plant growth regulators and soil properties on Miscanthus x giganteus biomass parameters and uptake of metals in military soils

2019 ◽  
Vol 34 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Diana Nebeská ◽  
Valentina Pidlisnyuk ◽  
Tatyana Stefanovska ◽  
Josef Trögl ◽  
Pavlo Shapoval ◽  
...  
Keyword(s):  
Plant Growth ◽  
Soil Properties ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Combined Treatment ◽  
Plant Roots ◽  
Biogenic Elements ◽  
Miscanthus X Giganteus ◽  
Unusual Behavior ◽  
The Impact

Abstract The impact of plant growth regulators (PGRs) “Stimpo” and “Regoplant” on Miscanthus x giganteus (Mxg) biomass parameters was investigated when the plant was grown in military soils with different properties from Dolyna, Ukraine and Hradcany, Czech Republic. The results showed that PGRs positively influenced the biomass parameters when the plant was grown in soil in Dolyna with good agricultural characteristics, the influence of “Regoplant” was higher and the best results were obtained with combined treatment: application to rhizomes before planting and spraying on the biomass during vegetation. Using of PGRs did not improve the biomass parameters when the plant was grown in poor soil in Hradcany. In parallel the peculiarities of the metals uptake process were studied for the following metals: chromium (Cr), manganese (Mn), nickel (Ni), copper (Cu), zinc (Zn), strontium (Sr) and lead (Pb). The uptake behavior of the monitored elements differed based on the soil quality. According to the bioconcentration factor uptake of the abiogenic elements, Cr and Pb, was dominant in the plant roots in both soils, whereas Ni was not detected in any plant tissues. The behavior of biogenic elements (Mn, Cu, Zn) and their analogs (Sr) was different. Those elements were more intensively taken up in shoot tissues in low-nutrient sandy Hradcany soils, while they were mainly taken up in plant roots in fertile Dolyna soils. The unusual behavior of biogenic elements in the low-nutrient soils may be explained by the effect of stress. However, more research is needed focused mainly on soil properties and nutrient availability in order to confirm or disprove this hypothesis and to explore the cause of the stress. The summarized results here show that soil properties influenced Mxg biomass parameters, affected the uptake behavior of metals significantly and tested PGRs cannot be utilized universally in the production of Mxg in the poor military soils.

scholarly journals Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings

2016 ◽  
Vol 68 (2) ◽  
pp. 399-404 ◽  
Author(s):  
Milan Dragicevic ◽  
Ana Simonovic ◽  
Milica Bogdanovic ◽  
Angelina Subotic ◽  
Nabil Ghalawenji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Expression Patterns ◽  
Nuclear Gene ◽  
Glutamate Synthase ◽  
Ammonium Assimilation ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
The Impact

Primary and secondary ammonium assimilation is catalyzed by the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway in plants. The Arabidopsis genome contains five cytosolic GS1 genes (GLN1;1 - GLN1;5), one nuclear gene for chloroplastic GS2 isoform (GLN2), two Fd-GOGAT genes (GLU1 and GLU2) and a GLT1 gene coding for NADH-GOGAT. Even though the regulation of GS and GOGAT isoforms has been extensively studied in response to various environmental and metabolic cues in many plant species, little is known about the effects of phytohormones on their regulation. The objective of this study was to investigate the impact of representative plant growth regulators, kinetin (KIN), abscisic acid (ABA), gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D), on the expression of A. thaliana GS and GOGAT genes. The obtained results indicate that GS and GOGAT genes are differentially regulated by growth regulators in shoots and roots. KIN and 2,4-D repressed GS and GOGAT expression in roots, with little effect on transcript levels in shoots. KIN affected all tested genes; 2,4-D was apparently more selective and less potent. ABA induced the expression of GLN1;1 and GLU2 in whole seedlings, while GA3 enhanced the expression of all tested genes in shoots, except GLU2. The observed expression patterns are discussed in relation to physiological roles of investigated plant growth regulators and N-assimilating enzymes.

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