Impact of Exogenous Plant Growth Regulators on Physiological Traits, Tendril and Flowering in Horsegram (Macrotyloma uniflorum Lam) under Kharif Season 

Author(s):  
R. Sivakumar

Background: Horsegram is an important pulse crop grown as Rabi crop in Tamil Nadu. Kharif sown crop could not flowers may be because of more number of tendrils produced as compare to Rabi sown crop. Hence, an attempt was made to reduce the number of tendrils and induce flowering in horsegram during Kharif season by plant growth regulators. Methods: A field experiment was conducted to study the impact of plant growth regulators viz., salicylic acid (100 ppm), chlormequat chloride (CCC - 250 ppm), mepiquat chloride (250 ppm), tri iodo benzoic acid (TIBA - 200 ppm) and nitrobenzene (0.2%) on physiological traits and tendril growth associated with flowering in horsegram during Kharif. Different treatments were applied through foliar application at 25 Days after sowing. Result: Among the PGRs, foliar application of TIBA registered the lowest number of tendrils (2.3) followed by CCC (3.0) compared to other treatments. Higher root length of 16.8 cm and RWC of 82.3% was found in CCC treatment. CCC also registered the highest photosynthetic rate (27.15 µmol m-2 s-1), transpiration rate (18.06 mmol m-2 s-1) and lowest leaf temperature (26°C) compared to other treatments. The highest soluble protein content of 13.1 mg g-1 was also estimated in CCC treatment followed by mepiquat chloride (12.51 mg g-1). Sucrose phosphate synthase (SPS) activity did not show any significant difference between the treatments. Number of flowers buds formed per plant was zero and hence the flowering did not take place in any treatments. Among the plant growth regulators used, TIBA and CCC registered its positive action on reduced the number of tendrils and leaf temperature, but not enough to induce flowering under Kharif season.

2017 ◽  
Vol 48 (1) ◽  
Author(s):  
Al-Sahaf & et al.

This research was conducted to study the impact of foliar spraying by some plant growth regulators to increase salts tolerance of date palm (Phoenix dactylifera L.) cv. Shakar. Thirty young date palm trees (seven years old) and same number of fruiting trees (18 years old) were selected and spraying with naphthalene acetic acid (NAA) solutions at the concentrations (0.50, 100 and 150 ppm), Salicylic acid (0, 0.25, 0.50, and 1.0 mM) and putrescine (0, 0.025, 0.050, 0.1 mM). Results indicated that the application of growth regulators led to reduction trunk diameter comparing with control (121.33 cm). Spraying NAA with concentration of 100 ppm, gave the highest height of the offshoots and a significant difference from the rest of the treatments (253.70 cm). Spraying putrescine with 1.0 mM concentration led to a significant increase in the number of leaves (fronds) as it increased to 24.33 leaf/offshoot, and leaf area which reached 691 cm2. Application of 0.25 mM Salisylic acid increased the number of bunches under saline conditions amounted to 12.67 bunch as well as increased the weight of the fruit to 8.82 g, which differed significantly from the control which gave 7.37 g. As for the size of the fruit, it was found that Spraying of NAA with 100 ppm led to increase the size of the fruit under saline conditions (8.67 cm). We can conclude that the spraying date palm with the above plant growth regulators under saline conditions can lead to increasing absorption of nutrients and subsequent growth and yield improvement.


Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 542
Author(s):  
Dariusz Kulus ◽  
Natalia Miler

Lamprocapnos spectabilis (L.) Fukuhara (bleeding heart) is valued both in the horticultural and pharmaceutical markets. Despite its great popularity, information on the in vitro tissue culture technology in this species is limited. There is also little knowledge on the application of plant extracts in the tissue culture systems of plants other than orchids. The aim of this study is to compare the utility of traditional plant growth regulators (PGRs) and natural extracts—obtained from the coconut shreds, as well as oat, rice, and sesame seeds—in the micropropagation and cryopreservation of L. spectabilis ‘Gold Heart’ and ‘White Gold’. The biochemical analysis of extracts composition is also included. In the first experiment related to micropropagation via axillary buds activation, the single-node explants were cultured for a 10-week-long propagation cycle in the modified Murashige and Skoog medium fortified either with 1.11 µM benzyladenine (BA) and 1.23 µM indole-3-butritic acid (IBA) or with 10% (v/v) plant extracts. A PGRs- and extract-free control was also considered. In the cryopreservation experiment, the same 10% (v/v) extracts were added into the medium during a seven-day preculture in the encapsulation-vitrification cryopreservation protocol. It was found that the impact of natural additives was cultivar- and trait-specific. In the first experiment, the addition of coconut extract favoured the proliferation of shoots and propagation ratio in bleeding heart ‘Gold Heart’. Rice extract, on the other hand, promoted callus formation in ‘White Gold’ cultivar and was more effective in increasing the propagation ratio in this cultivar than the conventional plant growth regulators (4.1 and 2.6, respectively). Sesame extract suppressed the development of the explants in both cultivars analysed, probably due to the high content of polyphenols. As for the second experiment, the addition of plant extracts into the preculture medium did not increase the survival level of the cryopreserved shoot tips (sesame and oat extracts even decreased this parameter). On the other hand, coconut extract, abundant in simple sugars and endogenous cytokinins, stimulated a more intensive proliferation and growth of shoots after rewarming of samples. Analysing the synergistic effect of conventional plant growth regulators and natural extracts should be considered in future studies related to L. spectabilis.


EUGENIA ◽  
2012 ◽  
Vol 21 (3) ◽  
Author(s):  
Stanley A.F. Walingkas ◽  
Meity Rantung

ABSTRACT This study aims to determine the effect of plant growth regulators and micro elements to cherelle wilt in cacao plants as well as finding the right business in tackling cherelle wilt in cacao plants. The research was carried out in the garden village folk Eris Eris Minahasa district for 8 months from January to August 2009. Spraying micro-nutrient elements is necessary to reduce cherelle wilt because the various treatments for the first observation is not significantly different, but the observations into two to seven observations to indicate a significant difference between treatments (percentage cherelle wilt on treatment without nutrient elements higher than sprayed). While spraying the treatment factor of plant growth regulators (NAA) is not necessary because the first observation and the two had no significant effect on the percentage of cherelle wilt affected only the third observation and further observations to a fourth, fifth, sixth and seventh again no effect. So is the treatment for fruit length and diameter showed that administration of the micro nutrients necessary for sprayed with micro nutrients result 22.10 cm higher than that is not sprayed is 19.43 cm. As for the diameter of the fruit is affected by plant growth regulators. Treatment is better than 200 ppm 100 ppm and 0 ppm (control). Keywords: NAA, cocoa, mikombi     ABSTRAK   Penelitian ini bertujuan mengetahui pengaruh pemberian zat pengatur tumbuh dan unsur mikro terhadap cherelle wilt pada tanaman kakao serta menemukan usaha yang tepat dalam menanggulangi cherelle wilt pada tanaman kakao. Penelitian ini dilaksanakan di kebun rakyat Desa Eris Kecamatan Eris Kabupaten Minahasa selama 8 bulan sejak Januari – Agustus 2009. Penyemprotan unsur hara mikro perlu dilakukan untuk mengurangi cherelle wilt sebab pada berbagai perlakuan untuk pengamatan pertama tidak berbeda nyata namun pada pengamatan ke dua sampai pengamatan ke tujuh menunjukkan adanya perbedaan nyata antar perlakuan (prosentase cherelle wilt pada perlakuan tanpa unsure hara lebih tinggi dari yang disemprot). Sementara faktor penyemprotan dengan perlakuan zat pengatur tumbuh (NAA) tidak perlu dilakukan sebab pengamatan pertama dan ke dua tidak berpengaruh nyata pada persentase cherelle wilt hanya berpengaruh pada pengamatan ke tiga dan selanjutnya pengamatan ke empat, ke lima, ke enam dan ke tujuh kembali tidak berpengaruh. Begitu juga perlakuan untuk panjang dan diameter buah menunjukkan bahwa pemberian unsur hara mikro perlu dilakukan sebab yang disemprot dengan unsur hara mikro hasilnya 22,10 cm lebih tinggi dari yang tidak disemprot yaitu 19,43 cm. Sementara untuk diameter buah dipengaruhi oleh zat pengatur tumbuh. Perlakuan 200 ppm lebih baik dari 100 ppm dan 0 ppm (kontrol). Eugenia Volume 18 No. 2  Agustus 2012 Kata kunci : NAA, kakao, mikombi


2019 ◽  
Vol 34 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Diana Nebeská ◽  
Valentina Pidlisnyuk ◽  
Tatyana Stefanovska ◽  
Josef Trögl ◽  
Pavlo Shapoval ◽  
...  

Abstract The impact of plant growth regulators (PGRs) “Stimpo” and “Regoplant” on Miscanthus x giganteus (Mxg) biomass parameters was investigated when the plant was grown in military soils with different properties from Dolyna, Ukraine and Hradcany, Czech Republic. The results showed that PGRs positively influenced the biomass parameters when the plant was grown in soil in Dolyna with good agricultural characteristics, the influence of “Regoplant” was higher and the best results were obtained with combined treatment: application to rhizomes before planting and spraying on the biomass during vegetation. Using of PGRs did not improve the biomass parameters when the plant was grown in poor soil in Hradcany. In parallel the peculiarities of the metals uptake process were studied for the following metals: chromium (Cr), manganese (Mn), nickel (Ni), copper (Cu), zinc (Zn), strontium (Sr) and lead (Pb). The uptake behavior of the monitored elements differed based on the soil quality. According to the bioconcentration factor uptake of the abiogenic elements, Cr and Pb, was dominant in the plant roots in both soils, whereas Ni was not detected in any plant tissues. The behavior of biogenic elements (Mn, Cu, Zn) and their analogs (Sr) was different. Those elements were more intensively taken up in shoot tissues in low-nutrient sandy Hradcany soils, while they were mainly taken up in plant roots in fertile Dolyna soils. The unusual behavior of biogenic elements in the low-nutrient soils may be explained by the effect of stress. However, more research is needed focused mainly on soil properties and nutrient availability in order to confirm or disprove this hypothesis and to explore the cause of the stress. The summarized results here show that soil properties influenced Mxg biomass parameters, affected the uptake behavior of metals significantly and tested PGRs cannot be utilized universally in the production of Mxg in the poor military soils.


Weed Science ◽  
1975 ◽  
Vol 23 (5) ◽  
pp. 428-432
Author(s):  
T. J. Allen ◽  
C. L. Leinweber ◽  
D. K. Prince ◽  
D. F. Bouchard

CBC (2,5-dichlorobenzoyl chloride) and MCB (methyl-2,5-dichlorobenzoate) function as plant growth regulators in a gaseous phase or in an aqueous solution. Sorghum [Sorghum bicolor (L.) Moench. ‘Martin’] and soybean [Glycine bicolor (L.) Merr. ‘Lee’] seeds were temporarily inhibited or delayed from germinating, but overcame the inhibition in time so there was no significant reduction in total germination. Seedlings produced from sorghum and soybean seed germinated and grown in aqueous solution of 35 μg/ml CBC or MCB were shorter and weighed less than control seedlings. Soybean seed treated with the vapors of CBC and MCB produced seedlings significantly reduced in dry weight as compared with that of controls. Foliar application of 500 or 1000 μg/ml of CBC significantly reduced the growth of soybean but not sorghum seedlings. Soybean seedlings treated with the vapors or an aqueous solution produced leaflets more narrow and thicker than control seedlings. A lethal response attributable to CBC or MCB was not recorded.


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