scholarly journals Nosema Ceranae Interactions with Nosema apis and Black Queen Cell Virus

Agriculture ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 963
Author(s):  
Anna Maria Gajda ◽  
Ewa Danuta Mazur ◽  
Andrzej Marcin Bober ◽  
Michał Czopowicz
Keyword(s):  
Apis Mellifera ◽  
Laboratory Tests ◽  
Laboratory Experiments ◽  
Genetic Material ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Black Queen Cell Virus ◽  
Queen Cell ◽  
Type C

Nosema ceranae is a relatively new pathogen of the honeybee (Apis mellifera) and the course of type C nosemosis (the disease that it causes) is not entirely known. In order to better understand the course and the consequences of this disease, laboratory experiments were performed. They aimed to compare the course of N. ceranae infection with the course of Nosema apis infection, taking its influence on the black queen cell virus (BQCV) into account. Determination of the quantity of N. ceranae and BQCV genetic material in laboratory tests was performed using real-time PCR. In mixed Nosema infections, N. ceranae “wins” the competition and manages to outnumber N. apis significantly. BQCV exacerbates the course of both A and C nosemoses, but the data shows that in the case of nosemosis C and this viral infection, the mortality rate was the highest from all examined groups. Obtained results show that N. ceranae is more pathogenic for A. mellifera than N. apis, and the course of type C nosemosis is much heavier, which results in the shortened life spans of bees, and in connection with BQCV it becomes even more dangerous to bees.

scholarly journals Brain transcriptomes of honey bees ( Apis mellifera ) experimentally infected by two pathogens: Black queen cell virus and Nosema ceranae

Genomics Data ◽  
2016 ◽  
Vol 10 ◽  
pp. 79-82 ◽  
Author(s):  
Vincent Doublet ◽  
Robert J. Paxton ◽  
Cynthia M. McDonnell ◽  
Emeric Dubois ◽  
Sabine Nidelet ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Nosema Ceranae ◽  
Black Queen Cell Virus ◽  
Queen Cell

scholarly journals Presence and Prevalence of Viruses in Local and Migratory Honeybees (Apis mellifera) in Massachusetts

2009 ◽  
Vol 75 (24) ◽  
pp. 7862-7865 ◽  
Author(s):  
Anna Welch ◽  
Francis Drummond ◽  
Sunil Tewari ◽  
Anne Averill ◽  
John P. Burand
Keyword(s):  
Apis Mellifera ◽  
Black Queen Cell Virus ◽  
Deformed Wing Virus ◽  
Sacbrood Virus ◽  
Queen Cell ◽  
The Impact

ABSTRACT Migratory and local bees in Massachusetts were analyzed for seven viruses. Three were detected: black queen cell virus (BQCV), deformed wing virus (DWV), and sacbrood virus (SBV). DWV was most common, followed closely by BQCV and then by SBV. BQCV and SBV were present at significantly higher rates in the migratory bees assayed, bringing into question the impact that these bees have on the health of local bee populations.

scholarly journals Dynamics of Nosema apis and Nosema ceranae Co-Infection Seasonally in Honey Bee (Apis mellifera L.) Colonies

2019 ◽  
Vol 63 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Asli Özkırım ◽  
Aygün Schiesser ◽  
Nevіn Keskin
Keyword(s):  
Apis Mellifera ◽  
Apis Cerana ◽  
Winter Season ◽  
Nosema Ceranae ◽  
Molecular Techniques ◽  
Winter Period ◽  
Nosema Apis ◽  
Infection Level ◽  
Seasonal Infection ◽  
Asian Honeybee

AbstractNosema apis is a pathogen spesific for the European honeybee, Apis mellifera L., while Nosema ceranae is specific for the Asian honeybee, Apis cerana. Turkey provides different environmental and host conditions for both Nosema species. The aim of the study is to determine the dynamic of N. cerenae and N. apis seasonal infection. A number of samples were collected from different apiaries between 2009-2016 years. The samples were kept at −20°C in the laboratory. Light microscopy was used for spore counting and molecular techniques were used to identify the Nosema species. The results showed that winter season had an impact on the type of Nosema as well as on infection rates. The number of N. ceranae spores decreases significantly at low temperatures (≤ 5°C). The winter period was found to be the main factor affecting nosema infection level and dominancy of Nosema ceranae. Furthermore, co-infection of both species is an indicator of the dynamics of N. apis and N. ceranae. This study suggests, that there is a dynamic prevalence among the Nosema species depending of the average winter temperature and not a replacement of N. apis by N. ceranae.

scholarly journals Population Genetics of Nosema apis and Nosema ceranae: One Host (Apis mellifera) and Two Different Histories

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145609 ◽  
Author(s):  
Xulio Maside ◽  
Tamara Gómez-Moracho ◽  
Laura Jara ◽  
Raquel Martín-Hernández ◽  
Pilar De la Rúa ◽  
...  
Keyword(s):  
Population Genetics ◽  
Apis Mellifera ◽  
Nosema Ceranae ◽  
Nosema Apis

scholarly journals The first report of the prevalence of Nosema ceranae in Bulgaria

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4252 ◽  
Author(s):  
Rositsa Shumkova ◽  
Ani Georgieva ◽  
Georgi Radoslavov ◽  
Daniela Sirakova ◽  
Gyulnas Dzhebir ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Molecular Detection ◽  
Ssu Rdna ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Pcr Assay ◽  
First Report ◽  
Duplex Pcr Assay ◽  
Different Parts

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in the honey bee Apis mellifera. The aim of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the area of Bulgaria. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest prevalence (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but as in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection of honey bee in Bulgaria. The results showed that N. ceranae is the main Nosema species in Bulgaria.

Comparison of the energetic stress associated with experimental Nosema ceranae and Nosema apis infection of honeybees (Apis mellifera)

2011 ◽  
Vol 109 (3) ◽  
pp. 605-612 ◽  
Author(s):  
Raquel Martín-Hernández ◽  
Cristina Botías ◽  
Laura Barrios ◽  
Amparo Martínez-Salvador ◽  
Aránzazu Meana ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Energetic Stress

scholarly journals Determination of Genetically Identical Strains of Four Honeybee Viruses in Bumblebee Positive Samples

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1310
Author(s):  
Ivan Toplak ◽  
Laura Šimenc ◽  
Metka Pislak Ocepek ◽  
Danilo Bevk
Keyword(s):  
Direct Sequencing ◽  
Rt Pcr ◽  
Black Queen Cell Virus ◽  
Queen Cell ◽  
Pcr Products ◽  
Acute Bee Paralysis Virus ◽  
Bee Virus ◽  
Paralysis Virus ◽  
High Diversity

In recent years, there has been growing evidence that certain types of honeybee viruses could be transmitted between different pollinators. Within a voluntary monitoring programme, 180 honeybee samples (Apis mellifera carnica) were collected from affected apiaries between 2007 and 2018. Also from August 2017 to August 2018, a total 148 samples of healthy bumblebees (Bombus lapidarius, B. pascuorum, B. terrestris, B. lucorum, B. hortorum, B. sylvarum, B. humilis) were collected at four different locations in Slovenia, and all samples were tested by using RT-PCR methods for six honeybee viruses. Direct sequencing of a total 158 positive samples (acute bee paralysis virus (ABPV n = 33), black queen cell virus (BQCV n = 75), sacbrood bee virus (SBV n = 25) and Lake Sinai virus (LSV n = 25)) was performed from obtained RT-PCR products. The genetic comparison of identified positive samples of bumblebees and detected honeybee field strains of ABPV, BQCV, SBV, and LSV demonstrated 98.74% to 100% nucleotide identity between both species. This study not only provides evidence that honeybees and bumblebees are infected with genetically identical or closely related viral strains of four endemically present honeybee viruses but also detected a high diversity of circulating strains in bumblebees, similar as was observed among honeybees. Important new genetic data for endemic strains circulating in honeybees and bumblebees in Slovenia are presented.

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