scholarly journals Metabonomic Profiling Analyses Reveal ANS Upregulation to Enhance the Flavonoid Pathway of Purple-Fleshed Sweet Potato Storage Root in Response to Deep Shading

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 737
Author(s):  
Ying He ◽  
Dan Zhu ◽  
Yujun Sun ◽  
Qian Wang ◽  
Lan Zhu ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Soluble Sugars ◽  
Economic Benefits ◽  
Mass Spectrometry Analysis ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Potato Storage ◽  
Storage Root ◽  
Flavonoid Pathway ◽  
Storage Roots

Intercropping, as a common worldwide cultivation pattern, provides opportunities for sustainable agriculture with fuller use of light, temperature and land resources and greater yield per unit of land. The intercropping impact on crop quality is a current focus. This study found that shading cultivation of purple-fleshed sweet potato can improve the storage root pigment accumulation by more than 20% to increase economic benefits. We performed gas chromatography and mass spectrometry analysis of storage roots of the anthocyanin-enriched cultivar Jihei-1 under 60% shading and nonshaded treatments. A total of 224 differential metabolites were identified, among which N-acetyl-5-hydroxytryptamine, 1-monopalmitin, 4-pyridoxic acid, dodecano, arbutin, tryptophan, citrulline and phenylalanine were significantly upregulated under shading with a more than 10-fold change. Furthermore, metabolic pathway enrichment maps were based on the biological processes and stratification level selected. These metabolites mainly influenced the pathways of phenylpropanoid biosynthesis, the citrate cycle, organic acid biosynthesis and metabolism and amino acid metabolism. Through tissue-specific dynamic changes in amino acids, soluble sugars, starch and anthocyanins during storage root development, we proposed a variety-specific strategy of purple-fleshed sweet potato in response to prolonged deep shading, that is, utilizing and enhancing broad aboveground-tissue photosynthesis and transferring photosynthates into roots in advance, leading to a rapid increase in storage root anthocyanin synthesis. With comprehensive qPCR, western blot and enzyme activity analyses, we identified three key enzymes, CHS, ANS and 3GT, in purple-fleshed sweet potato storage roots in response to shading, which affect the root anthocyanin content by influencing the flavonoid metabolism pathway. This study provides a theoretical basis for revealing the regulation of anthocyanin synthesis in crops and a guidance for high-quality sweet potato cultivation and nutritional improvement using shade facilities.

scholarly journals The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam]

2021 ◽  
Vol 12 ◽  
Author(s):  
Daowei Zhang ◽  
Yongjun Tan ◽  
Fang Dong ◽  
Ya Zhang ◽  
Yanlan Huang ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Anthocyanin Biosynthesis ◽  
Expression Patterns ◽  
Anthocyanin Content ◽  
Storage Root ◽  
Storage Roots ◽  
Cultivated Species ◽  
Purple Color ◽  
And Storage

IbMYB1 was one of the major anthocyanin biosynthesis regulatory genes that has been identified and utilized in purple-fleshed sweet potato breeding. At least three members of this gene, namely, IbMYB1-1, -2a, and -2b, have been reported. We found that IbMYB1-2a and -2b are not necessary for anthocyanin accumulation in a variety of cultivated species (hexaploid) with purple shoots or purplish rings/spots of flesh. Transcriptomic and quantitative reverse transcription PCR (RT-qPCR) analyses revealed that persistent and vigorous expression of IbMYB1 is essential to maintain the purple color of leaves and storage roots in this type of cultivated species, which did not contain IbMYB1-2 gene members. Compared with IbbHLH2, IbMYB1 is an early response gene of anthocyanin biosynthesis in sweet potato. It cannot exclude the possibility that other MYBs participate in this gene regulation networks. Twenty-two MYB-like genes were identified from 156 MYBs to be highly positively or negatively correlated with the anthocyanin content in leaves or flesh. Even so, the IbMYB1 was most coordinately expressed with anthocyanin biosynthesis genes. Differences in flanking and coding sequences confirm that IbMYB2s, the highest similarity genes of IbMYB1, are not the members of IbMYB1. This phenomenon indicates that there may be more members of IbMYB1 in sweet potato, and the genetic complementation of these members is involved in the regulation of anthocyanin biosynthesis. The 3′ flanking sequence of IbMYB1-1 is homologous to the retrotransposon sequence of TNT1-94. Transposon movement is involved in the formation of multiple members of IbMYB1. This study provides critical insights into the expression patterns of IbMYB1, which are involved in the regulation of anthocyanin biosynthesis in the leaf and storage root. Notably, our study also emphasized the presence of a multiple member of IbMYB1 for genetic improvement.

Influence of volatiles from healthy and decaying sweet potato storage roots on sclerotial germination and hyphal growth of Sclerotium rolfsii

1989 ◽  
Vol 67 (1) ◽  
pp. 53-57 ◽  
Author(s):  
C. A. Clark
Keyword(s):  
Sweet Potato ◽  
Sclerotium Rolfsii ◽  
Hyphal Growth ◽  
Root Tissue ◽  
Natural Soil ◽  
Potato Storage ◽  
Storage Root ◽  
Storage Roots ◽  
Sweet Potatoes ◽  
Directional Growth

Volatiles released from sweet potato storage root tissue infected by different sweet potato storage root pathogens stimulated eruptive germination of sclerotia of Sclerotium rolfsii but did not influence the direction of hyphal growth on agarose. Volatiles from healthy sweet potato storage root tissue did not affect percent hyphal or eruptive germination of sclerotia of S. rolfsii but stimulated directional growth of hyphae toward the healthy tissue. In laboratory experiments, the frequency of infection of sweet potato stem segments by S. rolfsii on the surface of natural soil was increased when sclerotia were incubated in the presence of decaying sweet potato storage root tissue. Incidence of sclerotial blight lesions on sprouts in plant beds was increased in the presence of roots infected by Fusarium solani or Erwinia chrysanthemi. Volatiles from decaying sweet potato mother roots may predispose sweet potatoes to sclerotial blight.

Response mechanism of sweet potato storage root formation and bulking to soil compaction and its relationship with yield

2019 ◽  
Vol 45 (5) ◽  
pp. 755
Author(s):  
Wen-Qing SHI ◽  
Bin-Bin ZHANG ◽  
Hong-Juan LIU ◽  
Qing-Xin ZHAO ◽  
Chun-Yu SHI ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Soil Compaction ◽  
Root Formation ◽  
Potato Storage ◽  
Storage Root ◽  
Response Mechanism

Isolation and characterisation of invertase inhibitor from sweet potato storage roots

2008 ◽  
Vol 88 (15) ◽  
pp. 2615-2621 ◽  
Author(s):  
Guan-Jhong Huang ◽  
Ming-Jyh Sheu ◽  
Yuan-Shiun Chang ◽  
Te-Ling Lu ◽  
Heng-Yuan Chang ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Potato Storage ◽  
Storage Roots ◽  
Invertase Inhibitor

scholarly journals The use of humic acid urea fertilizer for increasing yield and utilization of nitrogen in sweet potato  

2017 ◽  
Author(s):  
Chen Xiaoguang ◽  
Kou Meng ◽  
Tang Zhonghou ◽  
Zhang Aijun ◽  
Li Hongmin
Keyword(s):  
Humic Acid ◽  
Sweet Potato ◽  
Production Efficiency ◽  
Potato Production ◽  
Utilization Rate ◽  
Nitrogen Accumulation ◽  
Storage Root ◽  
Nitrogen Absorption ◽  
Storage Roots ◽  
Urea Fertilizer

Humic acid urea fertilizer (HA-N) is a new type of slow-release nitrogenous fertilizer that can enhance utilization rate of urea, and consequently increases crop yield. However, there were few researches about the effect of HA-N on the nitrogen absorption and utilization in sweet potato production. Hence, the effect of HA-N on nitrogen accumulation and distribution, nitrogen use efficiency (NUE), and yield of sweet potato was studied in the field using the <sup>15</sup>N tracer technique. Results showed that HA-N significantly increased the number of storage roots per plant and the average fresh weight per storage root, as well as the yield increased by 29.6% compared with urea fertilizer. Furthermore, nitrogen accumulation of total plant was higher under the HA-N. In addition, HA-N significantly increased nitrogen production efficiency of fertilizer and nitrogen production efficiency. Results of a <sup>15</sup>N tracer experiment revealed that the percentage of nitrogen absorbed by plant from fertilizer increased from 31.1% to 38.7% and NUE increased from 33.5% to 44.8% with application of HA-N when compared with single N treatment, respectively. HA-N significantly increased sweet potato storage root yield, nitrogen absorption and NUE, as well as it reduced the loss of nitrogen fertilizer.  

scholarly journals Effect of cutting position and vine pruning level on yield of Sweet Potato (Ipomoea Batatas L.)

1970 ◽  
pp. 01-05
Author(s):  
Ncube Netsai ◽  
Mutetwa Moses, Mtaita Tuarira
Keyword(s):  
Sweet Potato ◽  
Root Length ◽  
Ipomoea Batatas ◽  
Block Design ◽  
Root Diameter ◽  
Root Weight ◽  
Stem Cuttings ◽  
Storage Root ◽  
Storage Roots ◽  
Significant Difference

There is significant variation in yield of storage roots and vines of sweet potato (Ipomoea batatas) among farmers due to use of different cutting positions and pruning of vines at different levels. This study was carried out to establish the cutting position and the vine pruning level that give the best yield of both the storage roots and vines. The study was conducted in a 3x3 factorial arrangement in Randomized Complete Block Design (RCBD) with three replications. Treatments included cutting position at three levels (apical cutting, middle cutting and basal cutting) and pruning at three levels, 0%, 25% and 50% respectively. Pruning was done. 50 days after planting. And storage root harvesting was done 100 days after planting. The two measurements were summed up to give the total vine weight. Storage root length, diameter and weight were measured at 100 DAP. Storage root length indicated significant difference (P<0.05) only among cutting positions with highest mean length (16.20 cm) obtained from apical cutting and the lowest (11.98 cm) from basal cutting. Storage root diameter, storage root weight and vine weight indicated significant interaction (P<0.05) of cutting position and vine pruning level. Highest mean root diameter and root weight were obtained from middle cutting and 25% vine pruning level, with the lowest being obtained from basal cutting and 50% vine pruning level. Highest vine weight was recorded from middle cutting and 50% vine pruning level, with the lowest being recorded from basal cutting and 0% vine pruning level. Both middle and apical stem cuttings can be recommended for higher storage root and vine yield. Vine pruning at 25% can be adopted for higher storage root yield while pruning at 50% can be suggested for higher vine yield.

Effects of Previous Insect Feeding Injury to Sweet Potato on Resistance to Sweet Potato Weevil (Coleoptera: Curculionidae) and Storage Root Chemistry

2003 ◽  
Vol 38 (1) ◽  
pp. 72-83
Author(s):  
Lixin Mao ◽  
Richard N. Story ◽  
Abner M. Hammond ◽  
Joseph K. Peterson ◽  
Don R. Labonte
Keyword(s):  
Caffeic Acid ◽  
Sweet Potato ◽  
Larval Survival ◽  
Pupal Weight ◽  
Storage Root ◽  
Storage Roots ◽  
Resin Glycoside ◽  
Adult Feeding ◽  
Sweet Potato Weevil ◽  
Potato Resistance

The effects of root and foliage feeding on sweet potato resistance to sweet potato weevil, Cylas formicarius (F.), and on the levels of resin glycoside and caffeic acid in sweet potato storage root periderm tissues were studied. Genotypes (“Beauregard,” “Excel,” “W-244,” “W250,” and “Sumor”) with varying levels of sweet potato weevil resistance were evaluated. Adult banded cucumber beetle, Diabrotica balteata LeConte, and larval Spodoptera latifascia (Walker) were introduced onto caged sweet potato plants in the field to elicit root feeding and defoliation on plants. Storage roots were evaluated for sweet potato weevil resistance by quantifying sweet potato weevil adult feeding, oviposition, larval survival, and pupal weight. Both root and foliage injuries were associated with an increase in oviposition (significant in 1998 but not in 1997), but there was no association with adult feeding, larval survival and pupal weight. Genotype had a significant effect on adult feeding, oviposition, and larval survival but not on pupal weight. Root and foliage injuries did not have a significant effect on the levels of resin glycoside and caffeic acid in storage roots. The levels of these compounds differed significantly among genotypes, but there was no apparent relationship between sweet potato weevil resistance (antibiosis) and the levels of these compounds.

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