The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam]

IbMYB1 was one of the major anthocyanin biosynthesis regulatory genes that has been identified and utilized in purple-fleshed sweet potato breeding. At least three members of this gene, namely, IbMYB1-1, -2a, and -2b, have been reported. We found that IbMYB1-2a and -2b are not necessary for anthocyanin accumulation in a variety of cultivated species (hexaploid) with purple shoots or purplish rings/spots of flesh. Transcriptomic and quantitative reverse transcription PCR (RT-qPCR) analyses revealed that persistent and vigorous expression of IbMYB1 is essential to maintain the purple color of leaves and storage roots in this type of cultivated species, which did not contain IbMYB1-2 gene members. Compared with IbbHLH2, IbMYB1 is an early response gene of anthocyanin biosynthesis in sweet potato. It cannot exclude the possibility that other MYBs participate in this gene regulation networks. Twenty-two MYB-like genes were identified from 156 MYBs to be highly positively or negatively correlated with the anthocyanin content in leaves or flesh. Even so, the IbMYB1 was most coordinately expressed with anthocyanin biosynthesis genes. Differences in flanking and coding sequences confirm that IbMYB2s, the highest similarity genes of IbMYB1, are not the members of IbMYB1. This phenomenon indicates that there may be more members of IbMYB1 in sweet potato, and the genetic complementation of these members is involved in the regulation of anthocyanin biosynthesis. The 3′ flanking sequence of IbMYB1-1 is homologous to the retrotransposon sequence of TNT1-94. Transposon movement is involved in the formation of multiple members of IbMYB1. This study provides critical insights into the expression patterns of IbMYB1, which are involved in the regulation of anthocyanin biosynthesis in the leaf and storage root. Notably, our study also emphasized the presence of a multiple member of IbMYB1 for genetic improvement.

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Potassium Fertilization Stimulates Sucrose-to-Starch Conversion and Root Formation in Sweet Potato (Ipomoea batatas (L.) Lam.)

International Journal of Molecular Sciences ◽

10.3390/ijms22094826 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4826

Author(s):

Yang Gao ◽

Zhonghou Tang ◽

Houqiang Xia ◽

Minfei Sheng ◽

Ming Liu ◽

...

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Intercellular Co2 Concentration ◽

Biomass Accumulation ◽

Starch Accumulation ◽

Storage Root ◽

Root Yield ◽

Starch Conversion ◽

And Storage ◽

Low K

A field experiment was established to study sweet potato growth, starch dynamic accumulation, key enzymes and gene transcription in the sucrose-to-starch conversion and their relationships under six K2O rates using Ningzishu 1 (sensitive to low-K) and Xushu 32 (tolerant to low-K). The results indicated that K application significantly improved the biomass accumulation of plant and storage root, although treatments at high levels of K, i.e., 300–375 kg K2O ha−1, significantly decreased plant biomass and storage root yield. Compared with the no-K treatment, K application enhanced the biomass accumulation of plant and storage root by 3–47% and 13–45%, respectively, through promoting the biomass accumulation rate. Additionally, K application also enhanced the photosynthetic capacity of sweet potato. In this study, low stomatal conductance and net photosynthetic rate (Pn) accompanied with decreased intercellular CO2 concentration were observed in the no-K treatment at 35 DAT, indicating that Pn was reduced mainly due to stomatal limitation; at 55 DAT, reduced Pn in the no-K treatment was caused by non-stomatal factors. Compared with the no-K treatment, the content of sucrose, amylose and amylopectin decreased by 9–34%, 9–23% and 6–19%, respectively, but starch accumulation increased by 11–21% under K supply. The activities of sucrose synthetase (SuSy), adenosine-diphosphate-glucose pyrophosphorylase (AGPase), starch synthase (SSS) and the transcription of Susy, AGP, SSS34 and SSS67 were enhanced by K application and had positive relationships with starch accumulation. Therefore, K application promoted starch accumulation and storage root yield through regulating the activities and genes transcription of SuSy, AGPase and SSS in the sucrose-to-starch conversion.

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Characterization of Anthocyanins in Sweet Potato Leaves Grown in Various Stages and Conditions

European Journal of Nutrition & Food Safety ◽

10.9734/ejnfs/2019/v10i430119 ◽

2019 ◽

pp. 253-262

Author(s):

Xiaoyu Su ◽

Zhenbao Jia ◽

Fei Tao ◽

Jiamin Shen ◽

Jingwen Xu ◽

...

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Anthocyanin Biosynthesis ◽

Total Phenolic ◽

Growth Stages ◽

Anthocyanin Content ◽

Potential Health ◽

Significant Difference ◽

Young Leaves ◽

Sweet Potato Leaves

Phytochemical-enriched edible greens, sweet potato leaves (Ipomoea batatas L.), have become popular due to potential health benefits. However, the phytochemical contents in sweet potato leaves and their subsequent change over harvest stages and growth condition are mostly unknown. In this study, the anthocyanin profile and content in leaves of four sweet potato cultivars, i.e., white-skinned and white-fleshed Bonita, red-skinned and orange-fleshed Beauregard, red-skinned and white-fleshed Murasaki and purple-skinned and purple-fleshed P40, were evaluated. Fourteen anthocyanins were isolated and identified by HPLC-MSI/MS. The most abundant was cyanidin 3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, which comprised up to 20% of the total anthocyanins. Of the young leaves (1st and 2nd slip cuttings), Bonita contained the highest anthocyanin content followed by P40. Of the mature leaves (vine stage), Beauregard had the greatest anthocyanin (592.5 ± 86.4 mg/kg DW) and total phenolic (52.2 ± 3 mg GAE/g DW). It should be noted that the lowest anthocyanin and total phenolic content of shoots were found in P40, while tubers of P40 contain the highest content of each. Furthermore, the increase in leaf anthocyanin content over the growth stages that was observed in three of the cultivars but not in P40. No significant difference of anthocyanin content was found in Beauregard leaves grown in the high tunnels when compared with that in the open field. This study demonstrated for the first time that anthocyanin levels were significantly changed in response to various growth stages but not high tunnel condition, indicating that the effect of anthocyanin biosynthesis in sweet potato leaves is highly variable and genotype specific.

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Sweetpotato Transplant Holding Duration Effects on Plant Survival and Yield

HortTechnology ◽

10.21273/horttech03808-17 ◽

2017 ◽

Vol 27 (6) ◽

pp. 818-823 ◽

Cited By ~ 1

Author(s):

William B. Thompson ◽

Jonathan R. Schultheis ◽

Sushila Chaudhari ◽

David W. Monks ◽

Katherine M. Jennings ◽

...

Keyword(s):

North Carolina ◽

Ipomoea Batatas ◽

Storage Root ◽

Storage Roots ◽

Higher Plant ◽

Root Numbers ◽

Dry Conditions ◽

Plant Stand ◽

And Storage ◽

Lower Plant

Studies were conducted in North Carolina to determine the effect of holding durations (HDs) [0, 1, 3, 5, and 7 days before planting (DBP)] of ‘Covington’ sweetpotato (Ipomoea batatas) transplants on plant stand and storage root numbers and yield in production fields. In a second field study, the effect of preplant irrigation (PI) treatments (PI and nonirrigation) were evaluated along with the transplant HD on plant stand, storage root numbers, and yield. Transplants held for 7 DBP did not survive as well as the other treatments (lower plant stands) and had lower no. 1, marketable, and total storage root numbers and yields than other holding treatments. HD of 1 or 3 DBP resulted in higher plant stands, and no. 1, marketable, and total numbers of storage roots and yields than holding for 0, 5, or 7 DBP. This study affirms the importance of soil moisture at and shortly after planting for transplant survival and yield. Holding transplants for 1–3 DBP can improve stand establishment and yields when dry conditions occur either before or soon after planting. However, holding transplants for 7 DBP can result in reduced plant stands and yields when stress/dry conditions occur soon after planting.

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Effect of cutting position and vine pruning level on yield of Sweet Potato (Ipomoea Batatas L.)

Journal of Aridland Agriculture ◽

10.25081/jaa.2019.v5.5255 ◽

1970 ◽

pp. 01-05

Author(s):

Ncube Netsai ◽

Mutetwa Moses, Mtaita Tuarira

Keyword(s):

Sweet Potato ◽

Root Length ◽

Ipomoea Batatas ◽

Block Design ◽

Root Diameter ◽

Root Weight ◽

Stem Cuttings ◽

Storage Root ◽

Storage Roots ◽

Significant Difference

There is significant variation in yield of storage roots and vines of sweet potato (Ipomoea batatas) among farmers due to use of different cutting positions and pruning of vines at different levels. This study was carried out to establish the cutting position and the vine pruning level that give the best yield of both the storage roots and vines. The study was conducted in a 3x3 factorial arrangement in Randomized Complete Block Design (RCBD) with three replications. Treatments included cutting position at three levels (apical cutting, middle cutting and basal cutting) and pruning at three levels, 0%, 25% and 50% respectively. Pruning was done. 50 days after planting. And storage root harvesting was done 100 days after planting. The two measurements were summed up to give the total vine weight. Storage root length, diameter and weight were measured at 100 DAP. Storage root length indicated significant difference (P<0.05) only among cutting positions with highest mean length (16.20 cm) obtained from apical cutting and the lowest (11.98 cm) from basal cutting. Storage root diameter, storage root weight and vine weight indicated significant interaction (P<0.05) of cutting position and vine pruning level. Highest mean root diameter and root weight were obtained from middle cutting and 25% vine pruning level, with the lowest being obtained from basal cutting and 50% vine pruning level. Highest vine weight was recorded from middle cutting and 50% vine pruning level, with the lowest being recorded from basal cutting and 0% vine pruning level. Both middle and apical stem cuttings can be recommended for higher storage root and vine yield. Vine pruning at 25% can be adopted for higher storage root yield while pruning at 50% can be suggested for higher vine yield.

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Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.115.2.288 ◽

1990 ◽

Vol 115 (2) ◽

pp. 288-293 ◽

Cited By ~ 11

Author(s):

Ajmer S. Bhagsari ◽

Doyle A. Ashley

Keyword(s):

Sweet Potato ◽

Harvest Index ◽

Ipomoea Batatas ◽

Field Experiments ◽

Storage Root ◽

Canopy Photosynthesis ◽

Storage Roots ◽

Physiological Basis ◽

Area Index ◽

Stage Of Development

Field experiments with 15 sweet potato [Ipomoea batatas L. (Lam.)] genotypes were conducted to study the physiological basis of yield in 1981 and 1982. The leaf area index differed significantly among the sweet potato genotypes during early and late phases of growth, hut showed an inconsistent relationship with yield. Single leaf net photosynthesis ranged from 0.74 to 1.12 mg CO2/m' per sec. Canopy photosynthesis for sweet potato genotypes differed significantly in 1981, but not in 1982. It ranged from 0.81 to 1.16 mg CO2/m2 per sec in Aug. 1981. and from 0.63 to 0.88 mg CO2/m2 per sec in 1982. Four hours after “C-labeling, 14C-assimilate translocation from the treated leaf ranged from 21% to 46%, but did not differ significantly among the genotypes. At final harvest, harvest index [HI, defined as (storage root yield/total biological yield) × 100] of the genotypes varied from 43% to 77% and 31% to 75% for 1981 and 1982, respectively. Canopy photosynthesis during September was significantly correlated with storage root dry matter yield (r = 0.54*) in 1981 and with phytomass (above-ground biomass plus storage roots) (r = 0.60*) in 1982. Both phytomass and HI were significantly correlated with storage root matter yield. Canopy photosynthetic evaluation of sweet potato germplasm may be-more relevant when the storage root sinks are at an advanced stage of development. Our study suggests that yield is poorly predicted by Pn, particularly when the genotypes have different leaf sizes.

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Morphological Variability and Storage Root Productivity of Some Sweet Potato (Ipomoea Batatas (L.) Lam) Genotypes in An Ultisol

International Journal Of Scientific Advances ◽

10.51542/ijscia.v2i6.20 ◽

2021 ◽

Vol 2 (6) ◽

Author(s):

Gamaliel I. Harry ◽

Joseph I. Ulasi

Keyword(s):

Principal Component Analysis ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Principal Component ◽

Component Analysis ◽

The Other ◽

Storage Root ◽

Root Yield ◽

Cropping Seasons ◽

And Storage

Ten sweet potato (Ipomoea batatas (L.) Lam) genotypes sourced from National Root Crops Research Institute, Umudike were evaluated under rainfed condition in 2020 and 2021 cropping seasons at the Teaching and Research Farm of the University of Uyo, Uyo, Akwa Ibom State to ascertain variability among ten sweet potato genotypes and identify traits which are positively and significantly associated with yield and also identify genotypes with high yield potential for cultivation on an ultisol of Akwa Ibom State, Nigeria. The ten genotypes: TIS87/0087, Naspoy-12, Umuspo-4, Umuspo-1, Naspoy-11, Lourdes, Erica, Delvia, Ex-Igbariam and Umuspo-3 were used as treatments and the experiment was laid out in a randomized complete block design with three replications. Data collected were subjected to analysis of variance, correlation and principal component analysis. The genotype differs significantly (P≤ 0.05) for number of marketable roots, weight of marketable roots and fresh roots yield. UMUSPO-3 was superior over all the other genotypes for the following character; number of marketable roots, weight of marketable root yield and fresh root yield. Umuspo-3 produced the highest storage root yield (28.78t/ha, 27.09t/ha) in 2020 and 2021 cropping seasons, respectively. The result of the correlation analysis also revealed that vine length, number of marketable roots, weight of marketable were highly significantly and positively (P<0.01) correlated with fresh root yield. Principal component analysis (PCA) had four main principal components explaining 81.55% of the total variation with number of marketable roots, weight of marketable tuber and storage root yield contributing the most to the first PCA. Umuspo-3 outperformed the other nine sweet potato genotypes in yield and yield related characters. Therefore, Umuspo-3 been a high yielding genotype adaptable to Uyo agro-ecology, could be recommended to sweet potato growers for fresh storage root production.

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Effect of planting methods on growth and yield of sweet potato (Ipomoea batatas L.) varieties at Luyengo, midlevel of Eswatini

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2021.11.1.0281 ◽

2021 ◽

Vol 11 (1) ◽

pp. 013-021

Author(s):

Sakhile Sipho Dlamini ◽

Mzwandile Petros Mabuza ◽

Bonginkhosi Edward Dlamini

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Growth And Yield ◽

Storage Root ◽

Root Yield ◽

Storage Roots ◽

Potato Varieties ◽

Planting Methods ◽

Method Of Planting ◽

Planting Method

Sweet potato (Ipomoea batatas L.) is the most grown storage root crop in Eswatini. However, its storage root yield is low among smallholder farmers partly due to use of inappropriate varieties and agronomic practices such as planting method. Thus, a field experiment was conducted at the University of Eswatini, Faculty of Agriculture, Luyengo, during 2019/2020 cropping season to determine the effects of planting method on growth and yield of the three sweet potato varieties. Two planting methods, namely horizontal and vertical; and three sweet potato varieties, namely Kenya-white, Ligwalagwala and Lamngititi were evaluated in a factorial arrangement in randomized complete block design in three replications. Results showed non-significant difference between the planting methods in most growth and yield parameters recorded for the sweet potato varieties. However, the vertical method of planting had relatively higher vine length, number of branches, mass of storage roots and storage root yield than the horizontal method. On the other hand, there were significant (P<0.05) differences among the sweet potato varieties for most of parameters recorded. The sweet potato variety Ligwalagwala had the highest vine length, number of storage roots per plant (6.47), mass of storage roots per plant (1137 g) and storage root yield (12.01 tonnes/ha). Thus, either horizontal or vertical method of planting and variety Ligwalagwala can be used to increase the productivity of sweet potato in the study area.

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EFFECT OF GYPSUM ON EARLY GROWTH AND YIELD OF SWEET POTATO.

HortScience ◽

10.21273/hortsci.25.9.1078a ◽

1990 ◽

Vol 25 (9) ◽

pp. 1078a-1078

Author(s):

E. Niyonsaba ◽

E. G. Rhoden ◽

P. K. Biswas ◽

G.W. Carver

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Dry Matter ◽

Block Design ◽

Early Growth ◽

Growth And Yield ◽

Root Weight ◽

Storage Root ◽

Root Yield ◽

And Storage

A study was conducted to assess the effects of gypsum on the early growth and storage root yield of sweet potato (Ipomoea batatas) cvs `Jewel', `Goergia Jet' and `TI-155'. Three rates of gypsum were applied (1.03, 2.06 and 3.09 tons/acre). These represented half, recommended and 1.5 recommended levels. The experiment was a randomized complete block design with a split plot arrangement of treatment. Leaf area, total dry matter, leaf dry matter and stat-age root weight were recorded at 30-day intervals. Plants receiving half the recommended levels of gypsum produced the highest total storage root dry matter (0.306 t/a) and the highest leaf dry matter (0.116 t/a). Although a positive relationship exists between leaf dry matter and storage root yield between 90 and 120 days, there was no such relationship between those parameters either at 30 and 60 days or 60 and 90 days after transplanting.

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Sweetpotato `Beauregard' Mericlones Vary in Yield, Vine Characteristics, and Storage Root Size and Shape Attributes

HortScience ◽

10.21273/hortsci.38.6.1089 ◽

2003 ◽

Vol 38 (6) ◽

pp. 1089-1092 ◽

Cited By ~ 2

Author(s):

A.Q. Villordon ◽

J.M. Cannon ◽

H.L. Carroll ◽

J.W. Franklin ◽

C.A. Clark ◽

...

Keyword(s):

Ipomoea Batatas ◽

Maximum Yield ◽

Storage Root ◽

Storage Roots ◽

Marketable Yield ◽

Methods Of Evaluation ◽

Foundation Seed ◽

Axis Length ◽

And Storage ◽

Selection Of

Yield tests and evaluation of selected storage root and vine characters were conducted among 12 `Beauregard' sweetpotato [Ipomoea batatas (L.) Lam.] mericlones. Maximum yield differences were 43%, 48%, 79%, and 40% for U.S. #1, canners, jumbos, and total marketable yield, respectively. Additive main effect and multiplicative interaction (AMMI) biplot analysis was useful in graphically presenting the yield differences and stability patterns of mericlones. Differences were also detected in vine length, internode diameter, and internode length. Digital image analysis of U.S. #1 storage roots also revealed differences in storage root minor axis length, roundness, and elongation attributes. The results provide valuable information for enhancing current methods of evaluation and selection of mericlones for inclusion in sweetpotato foundation seed programs.

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