scholarly journals Marker-Assisted Pyramiding of Multiple Disease Resistance Genes in Coffee Genotypes (Coffea arabica)

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1763
Author(s):  
Dênia Pires de Almeida ◽  
Eveline Teixeira Caixeta ◽  
Karoliny Ferreira Moreira ◽  
Antonio Carlos Baião de Oliveira ◽  
Kátia Nogueira Pestana de Freitas ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Fragment Length Polymorphism ◽  
Caps Marker ◽  
Coffee Berry ◽  
Hemileia Vastatrix ◽  
Sequence Characterized Amplified Region ◽  
F2 Population ◽  
Coffee Leaf Rust ◽  
Multiple Disease Resistance

The use of resistant cultivars is the most effective strategy for controlling coffee leaf rust caused by the fungus Hemileia vastatrix. To assist the development of such cultivars, amplified fragment-length polymorphism (AFLP) markers linked to two loci of coffee resistance to races I and II as well as pathotype 001 of H. vastatrix were converted to sequence-characterized amplified region (SCAR) and cleaved amplified polymorphic site (CAPS) markers. In total, 2 SCAR markers and 1 CAPS marker were validated in resistant and susceptible parents as well as in 247 individuals from the F2 population. The efficiency of these markers for marker-assisted selection (MAS) was evaluated in F2:3 and backcross (BCrs2) populations genotyped with the developed markers and phenotyped with race II of H. vastatrix. The markers showed 90% efficiency in MAS. Therefore, the developed markers, together with molecular markers associated with other rust resistance genes, were used for F3:4 and BCrs3 coffee selection. The selected plants were analyzed using two markers associated with coffee berry disease (CBD) resistance, aiming for preventive breeding. MAS of F3:4 and BCrs3 individuals with all resistance loci was feasible. Our phenotypic and genotypic approaches are useful for the development of coffee genotypes with multiple genes conferring resistance to coffee leaf rust and CBD.

scholarly journals A fungus-eat-fungus world: Digitopodium, with particular reference to mycoparasites of the coffee leaf rust, Hemileia vastatrix

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Adans A. Colmán ◽  
Harry C. Evans ◽  
Sara S. Salcedo-Sarmiento ◽  
Uwe Braun ◽  
Kifle Belachew-Bekele ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Democratic Republic Of Congo ◽  
Sequence Data ◽  
Molecular Study ◽  
Classical Biological Control ◽  
Coffea Canephora ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust

AbstractDigitopodium hemileiae was described originally in 1930 as Cladosporium hemileiae; growing as a mycoparasite of the coffee leaf rust (CLR), Hemileia vastatrix, in a sample of diseased leaves of Coffea canephora collected in the Democratic Republic of Congo. No cultures from this material exist. More recently, the type material was re-examined and, based on morphological features, considered to be incorrectly placed in Cladosporium. The new genus Digitopodium was erected to accommodate this species. Interest in fungal antagonists of H. vastarix, as potential biocontrol agents of CLR, led to comprehensive surveys for mycoparasites, both in the African centre of origin of the rust, as well as in its South American exotic range. Among the rust specimens from Ethiopia, one was found to be colonized by a fungus congeneric with, and similar to, D. hemileiae. Pure cultures obtained from the Ethiopian material enabled a molecular study and for its phylogenetic position to be elucidated, based on DNA sequence data from the ITS and LSU regions. Molecular data showed that two members of the recently erected genus Hyalocladosporiella (Herpotrichiellaceae: Chaetothyriales) are congeneric with Digitopodium from Ethiopia and morphologically similar to both D. hemileiae and the two Ethiopian isolates. These isolates were found to be morphologically and genetically identical to H. tectonae, described previously from Brazil. Thus, species of Hyalocladosporiella are re-allocated to Digitopodium here; including D. tectonae, and a novel species, D. canescens, recently found in Brazil growing as a mycoparasite of Puccinia thaliae. The potential use of D. hemileiae and D. tectonae for classical biological control of CLR is discussed.

scholarly journals Structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: a functional marker based approach

2019 ◽  
Author(s):  
Geleta Dugassa Barka ◽  
Eveline Teixeira Caixeta ◽  
Sávio Siqueira Ferreira ◽  
Laércio Zambolim
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Function Analysis ◽  
Bac Library ◽  
Leaf Rust Resistance ◽  
Molecular Data ◽  
Coffee Leaf Rust ◽  
Rust Resistance Genes

AbstractPhysiology-based differentiation of SH genes and Hemileia vastatrix races is the principal method employed for the characterization of coffee leaf rust resistance. Based on the gene-for-gene theory, nine major rust resistance genes (SH1-9) have been proposed. However, these genes have not been characterized at the molecular level. Consequently, the lack of molecular data regarding rust resistance genes or candidates is a major bottleneck in coffee breeding. To address this issue, we screened a BAC library with resistance gene analogs (RGAs), identified RGAs, characterized and explored for any SH related candidate genes. Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. Furthermore, comparative analysis of the two RGAs belonging to CC-NBS-LRR revealed more intense diversifying selection in tomato and grape genomes than in coffee. For the first time, the present study has unveiled novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding. The characterized candidate RGA is of particular importance for further biological function analysis in coffee.

scholarly journals Stability of Arabica coffee genotype (Coffea arabica L.) against leaf rust (Hemileia vastatrix)

2021 ◽  
Vol 748 (1) ◽  
pp. 012002
Author(s):  
Sabam Malau ◽  
Albiner Siagian ◽  
Maria Rumondang Sihotang
Keyword(s):  
Leaf Rust ◽  
Block Design ◽  
Research Result ◽  
Dry Season ◽  
Environment Interaction ◽  
Rust Disease ◽  
Hemileia Vastatrix ◽  
Arabica Coffee ◽  
Coffee Leaf Rust ◽  
Rust Severity

Abstract Coffee is now experiencing a serious threat from fungus Hemileia vastatrix which caused epidemic of rust disease in America, Africa, and Asia. As solution, the use of resistant cultivars is the best way. However, interaction between genotype and environment can change the rank of genotypes that shows instability of these genotypes against leaf rust. Purpose of this research was to study stability of genotypes of Arabica coffee against coffee leaf rust. A field experiment was arranged as factorial randomized complete block design with 2 factors (genotypes and climate zones) with three replication. The observed parameters were branch rust incidence, leaf rust incidence, and leaf rust severity. This research result showed significant genotype x environment interaction in all variables. Length of dry season is the most important factor affecting coffee leaf rust because it had the highest correlation coefficient with leaf rust severity (r = 0.662**). Less length of dry season should be the first criteria for selection of coffee farms. The most desired genotype was G7 which performed low leaf rust severity (7.71%) and had a stable resistance indicated by the same leaf rust severity in all environments and 6 SMg. Due to the significant interaction between genotypes and the environment, the genotype to be planted in a region must be tested in that region first.

scholarly journals Seasonal and altitudinal differences in coffee leaf rust epidemics on coffee berry disease-resistant varieties in Southwest Ethiopia

2019 ◽  
Vol 44 (3) ◽  
pp. 244-250 ◽  
Author(s):  
Gerba Daba ◽  
Kenny Helsen ◽  
Gezahegn Berecha ◽  
Bart Lievens ◽  
Adugna Debela ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Coffee Berry ◽  
Southwest Ethiopia ◽  
Coffee Berry Disease ◽  
Disease Resistant ◽  
Resistant Varieties ◽  
Coffee Leaf Rust

scholarly journals Responses of Compact Coffee Clones Against Coffee Berry and Coffee Leaf Rust Diseases in Tanzania

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Deusdedit L. Kilambo ◽  
Shazia O. W. M. Reuben ◽  
Delphina P. Mamiro
Keyword(s):  
Leaf Rust ◽  
Coffee Berry ◽  
Rust Diseases ◽  
Coffee Leaf Rust

Non-host resistance responses of Arabidopsis thaliana to the coffee leaf rust fungus (Hemileia vastatrix)

Botany ◽  
2010 ◽  
Vol 88 (7) ◽  
pp. 621-629 ◽  
Author(s):  
Helena Gil Azinheira ◽  
Maria do Céu Silva ◽  
Pedro Talhinhas ◽  
Clara Medeira ◽  
Isabel Maia ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Rust ◽  
Rust Fungus ◽  
Hemileia Vastatrix ◽  
Callose Deposition ◽  
Mesophyll Cells ◽  
Model Plant ◽  
Heterologous System ◽  
Coffee Leaf Rust ◽  
Model Plant Arabidopsis Thaliana

Leaf rust, caused by Hemileia vastatrix Berk & Broome, is the most destructive fungal disease of coffee. In the absence of a suitable gene validation system in coffee, the objective of this study was to investigate whether the model plant Arabidopsis thaliana (L.) Heynh. may be used as a heterologous system for the molecular dissection of coffee responses to leaf rust. Histological examination of A. thaliana (Col-0) leaves inoculated with H. vastatrix (race II) showed that by 24 h after inoculation (hai), H. vastatrix uredospores differentiated appressoria and penetrated the stomata, but failed to form haustoria. Arabidopsis thaliana cellular resistance responses included hypersensitive-like response (HR) of stomata guard cells together with accumulation of phenolic compounds and callose deposition in walls of epidermal and mesophyll cells. Results indicate that H. vastatrix infection triggered the induction of a set of defence-related genes peaking at 18 and 42 hai. The non-host HR triggered by H. vastatrix in the model plant A. thaliana makes it usable to infer the function of coffee genes involved in pre-haustorial rust resistance.

Evaluation of Different Botanicals for the Control of Coffee Leaf Rust (Hemileia vastatrix Berkeley and Broome)

2017 ◽  
Vol 14 (6) ◽  
pp. 1-8
Author(s):  
R Mudyiwa ◽  
N Mwatsiya ◽  
B Manenji ◽  
P Chidoko ◽  
C Mahoya
Keyword(s):  
Leaf Rust ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust

Sensitization to the fungus Hemileia vastatrix (coffee leaf rust)

Allergy ◽  
2001 ◽  
Vol 56 (7) ◽  
pp. 684-687 ◽  
Author(s):  
M. A. Croce Portocarrero ◽  
E. Rosa da Costa Manso ◽  
W. Gambale ◽  
L. Takayama ◽  
C. E. Oliveira Andrade ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust

scholarly journals Current status on coffee leaf rust (hemileia vastatrix) in sidama and gedeo zone, southern Ethiopia

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Ano Wariyo ◽  
Habtamu Gebreselassie ◽  
Wondmagegnehu Gerbatsedik ◽  
Kifle Belachew
Keyword(s):  
Leaf Rust ◽  
Disease Incidence ◽  
Regression Equation ◽  
Current Status ◽  
Southern Ethiopia ◽  
Hemileia Vastatrix ◽  
Resistant Varieties ◽  
Coffee Leaf Rust ◽  
Control Disease ◽  
Extent Of Damage

Coffee is the most important and second traded commodity next to oil in the world. In Ethiopia, coffee leaf rust caused by Hemileia vastatrix is one of the third most economically important diseases of Coffee Arabica. The current status of coffee leaf rust was intensively assessed and examined in 189 sample coffee farms from six districts across major coffee growing areas of the Southern Ethiopia. At each farm, ten randomly selected trees on a diagonal transect across the farm were assessed for disease incidence and severity. The survey data showed that coffee leaf rust was present in all assessed district varying in magnitude and extent of damage. The highest overall mean of rust incidence (38.6%) and severity (13.80%) was recorded at Dilla zuria while the lowest incidence (10.52%) and severity (1.38%) was at Yergachaffee district. The highest altitude range was obtained at Yergachaffee (1838-2056) while the lowest was at Dilla zuria (1434-1825) district. Overall mean severity and incidence result indicate that, there was statistical (p = 0.058 for severity, p = 0.044 for incidence) and negative correlation between intensity and altitude (severity, r= -0.80 and incidence, r= -0.82). The linear regression equation of severity and incidence obtained from the data was Y= -0.036X+73.16 and Y = -0.074X +158.9 (where Y= percentage CLR severity and incidence, respectively and X= altitude). This regression equation implies that, in higher elevation areas the rust intensity was low as compare to the lower elevation. Finally, the disease was remarkably increasing from time to time in all assessed coffee farms. Therefore, appropriate measurement like farmers’ awareness creation and training on how to control disease as well as appropriate agronomic managements has to be seriously undertaken. In addition, development of coffee leaf rust resistant varieties to southern Ethiopia through resistant breeding has to be set high priority.

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