scholarly journals Interactive Effects of Biochar, Nitrogen, and Phosphorous on the Symbiotic Performance, Growth, and Nutrient Uptake of Soybean (Glycine max L.)

Agronomy ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 27
Author(s):  
Dilfuza Egamberdieva ◽  
Hua Ma ◽  
Moritz Reckling ◽  
Richard Ansong Omari ◽  
Stephan Wirth ◽  
...  
Keyword(s):  
Glycine Max ◽  
Plant Growth ◽  
Bradyrhizobium Japonicum ◽  
Dry Weight ◽  
P Uptake ◽  
Interactive Effects ◽  
Shoot Biomass ◽  
Nodule Number ◽  
Glycine Max L ◽  
P Supply

Numerous studies reported the positive effect of soil amendment with biochar on plant development. However, little is known about biochar and its interrelation with nitrogen (N) and phosphorous (P) additions and their impact on plant growth. We carried out greenhouse experiments to understand the interactive effects of nitrogen and phosphorus supply, as well as biochar amendment, on the symbiotic performance of soybean (Glycine max L.) with Bradyrhizobium japonicum, and plant growth and nutrient uptake. The biochar was produced from maize by heating at 600 °C for 30 min and used for pot experiments at an application rate of 2%. Plants were fertilized with two different concentrations of P (KH2PO4) and N (NH4NO3). Biochar application significantly increased the dry weight of soybean root and shoot biomass, by 34% and 42%, under low nitrogen and low phosphorus supply, respectively. Bradyrhizobium japonicum inoculation enhanced the dry weight of shoot biomass significantly, by 41% and 67%, in soil without biochar and with biochar addition, respectively. The nodule number was 19% higher in plants grown under low N combined with low or high P, than in high N combinations, while biochar application increased nodule number in roots. Moreover, biochar application increased N uptake of plants in all soil treatments with N or P supply, compared with B. japonicum-inoculated and uninoculated plants. A statistical difference in P uptake of plants between biochar and nutrient levels was observed with low N and high P supply in the soil. Our results show that the interactions between nitrogen, phosphorus, and biochar affect soybean growth by improving the symbiotic performance of B. japonicum and the growth and nutrition of soybean. We observed strong positive correlations between plant shoot biomass, root biomass, and N and P uptake. These data indicated that the combined use of biochar and low N, P application can be an effective approach in improving soybean growth with minimum nutrient input.

Effect of rj1rj1 (non-nodulating) soybeans on nodulation of near isogenic Rj1Rj1 plants in nutrient culture

1977 ◽  
Vol 23 (8) ◽  
pp. 988-993 ◽  
Author(s):  
D. L. Eskew ◽  
L. E. Schrader
Keyword(s):  
Glycine Max ◽  
Nitrate Uptake ◽  
Dry Weight ◽  
Near Isogenic Lines ◽  
Nodule Number ◽  
Nutrient Culture ◽  
Glycine Max L ◽  
Plant Grown ◽  
Highly Correlated ◽  
Nutrient Solutions

An earlier proposal (Can. J. Microbiol. 7: 851; 1961) that rj1rj1 (non-nodulating) soybeans (Glycine max (L.) Merr.) excrete a substance that inhibits nodulation of Rj1Rj1 (nodulating) plants was tested. Using near isogenic lines (isolines) of 'Clark' and 'Harosoy' soybeans, we consistently found nonsignificant reductions in nodule number and acetylene reduction per Rj1Rj1 plant grown in association with their rj1rj1 counterparts; these results suggest that a nodulation inhibitor is not associated with the rj1 gene. Reducing the number of plants grown in each pot produced significant (P = 0.05) reductions in nodule number per Rj1Rj1 plant, and resembled the observations of the earlier report. On this basis, we suggest that the reported inhibition of nodulation was due to a failure to detoxify or remove an inhibitor (possibly nitrate) already present in the nutrient solution. Both Clark isolines removed nitrate from their nutrient solutions at similar rates. Harosoy rj1rj1 plants removed nitrate at a significantly (P = 0.05) slower rate than Harosoy Rj1Rj1 plants, but the differences were not correlated (P = 0.05) with the small observed decreases in nodulation. These differences in nitrate uptake were highly correlated (P = 0.01) with reduced dry weight per Harosoy rj1rj1 plant.

The nitrate-tolerant symbiosis of Glycine max (L.) Merr. nts382 and Bradyrhizobium japonicum is also tolerant of ammonium

2000 ◽  
Vol 77 (10) ◽  
pp. 1432-1438
Author(s):  
J Kevin Vessey ◽  
Bert Luit
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Continuous Flow ◽  
Dry Weight ◽  
Hydroponic Culture ◽  
Wild Type ◽  
Whole Plant ◽  
In The Wild ◽  
Glycine Max L ◽  
Wild Type Parent

Previously, we have shown that the Glycine max (L.) Merr. -Bradyrhizobium japonicum symbiosis is very sensitive to inhibition by NH4+. The current study addresses whether the supernodulating soybean mutant, nts382, which is known to be tolerant of NO3-, is also tolerant of NH4+. The nts382 mutant and its wild-type parent, Bragg, were grown in continuous-flow hydroponic culture in the presence of 0, 0.25, 0.5, or 1.0 mM 15N-enriched NH4+. Plants were harvested at 14, 21, and 28 days after inoculation. Both cultivars had the highest dry weight (DW) at each harvest date when grown on 0.25 mM NH4+. At 0.25 mM NH4+, whole plant DW increased by 5.3- and 3.2-fold in Bragg and nts382, respectively, compared with the 0.0 mM NH4+ control by the end of the experiment. As expected, whole-plant nodulation (nodules per plant), DW-specific nodulation (nodules per gram root dry weight), and nodule DW were severely inhibited in Bragg at all levels of NH4+. However, in nts382, whole-plant nodulation was not affected by NH4+ treatment, and nodule DW increased by as much as fivefold. Whereas DW-specific nodulation decreased by 94% in Bragg, this parameter decreased by only 52% in the nts382 mutant. Likewise, while the nitrogen derived from the atmosphere decreased by approximately 40% in NH4+-supplied Bragg, it increased 2.8-fold at 0.25 and 0.5 mM NH4+ in nts382. This study demonstrates that both nodulation and N2 fixation in nts382 are more tolerant of NH4+ than in the wild-type Bragg.

scholarly journals Reaction of soybean [Glycine max (L.) Merr.] to seed inoculation with Bradyrhizobium japonicum bacteria

2020 ◽  
Vol 66 (No. 5) ◽  
pp. 242-247
Author(s):  
Wacław Jarecki
Keyword(s):  
Glycine Max ◽  
Seed Yield ◽  
Total Protein ◽  
Bradyrhizobium Japonicum ◽  
Dry Weight ◽  
Total Protein Content ◽  
Seed Inoculation ◽  
Glycine Max L ◽  
Soybean Roots ◽  
Fat Yield

The aim of the study was to assess soybean response to sowing material inoculation with HiStick® Soy preparation, containing Bradyrhizobium japonicum. Based on the obtained results, it was found that the inoculation significantly increased the number and dry weight of nodules on soybean roots compared to control. The bacterial preparation significantly increased the number of pods per plant. As a result, a significant increase in seed yield (0.58 t/ha) was obtained compared to control. HiStick® Soy increased total protein content in seeds. Protein and fat yield was higher after seed inoculation by 318 kg/ha and 101 kg/ha, respectively, compared to control.

scholarly journals Interactive Effects of Nutrients and Bradyrhizobium japonicum on the Growth and Root Architecture of Soybean (Glycine max L.)

2018 ◽  
Vol 9 ◽  
Author(s):  
Dilfuza Egamberdieva ◽  
Dilfuza Jabborova ◽  
Stephan J. Wirth ◽  
Pravej Alam ◽  
Mohammed N. Alyemeni ◽  
...  
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Root Architecture ◽  
Interactive Effects ◽  
Glycine Max L

scholarly journals Changes in micronutrients, dry weight and plant growth of soybean (Glycine max L. Merrill) cultivars under salt stress

2008 ◽  
Vol 7 (11) ◽  
pp. 1650-1654 ◽  
Author(s):  
Tunccedil turk Murat ◽  
Tunccedil turk Ruveyde ◽  
Yasar Fikret
Keyword(s):  
Salt Stress ◽  
Glycine Max ◽  
Plant Growth ◽  
Dry Weight ◽  
Glycine Max L

Nodule Physiology of a Supernodulating Soybean (Glyine max) Mutant

1987 ◽  
Vol 14 (5) ◽  
pp. 527 ◽  
Author(s):  
AD Day ◽  
GD Price ◽  
KA Schuller ◽  
PM Gresshoff
Keyword(s):  
Plant Growth ◽  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Xylem Sap ◽  
Dry Weight ◽  
Acetylene Reduction Activity ◽  
Nodule Number ◽  
Reduction Activity ◽  
Inoculum Dose ◽  
Glycine Max L

The nodule physiology of a supernodulating, nitrate tolerant symbiosis soybean (Glycine max (L.) Merr.) mutant (nts382) was compared to that of its wild-type parent, cv. Bragg. Nodule number and mass were greater in nts382 than cv. Bragg and individual nodule mass, bacteroid and haem content, and acetylene reduction activity per g nodule were less. Acetylene reduction activity expressed per mg bacteroid protein was the same in the two genotypes. In median sections, the ratio of infected to total nodule area was smaller in nts382, infected cell size was smaller and there were fewer bacteroids per peribacteroid envelope. When inoculum dose was decreased from 109 to 103 viable cells per pot, nodule number on nts382 decreased approximately to that on cv. Bragg; nodule size, bacteroid and haem contents increased as did nodule acetylene reduction activity. Application of moderate levels of nitrate, which did not significantly affect symbiotic parameters of cv. Bragg or high inoculum nts382, stimulated nodule growth and nitrogenase activity of low inoculum nts382. A combination of nitrate and low inoculum levels enhanced nodule parameters of nts382 to the level usually seen with cv. Bragg. When supernodulated, plant dry weight of nts382 was less than that of Bragg; decreasing inoculum dose had no significant effect but nitrate application increased plant growth; nitrate plus low inoculum induced similar plant growth to that of cv. Bragg. Nodule carbohydrate content was similar in both genotypes but nodule and xylem sap ureide contents were higher in nts382. In general, nodules of supernodulated nts382 resembled under-developed cv. Bragg nodules; when supernodulation was avoided by using low inoculum doses, ,nts382 nodules resembled those of cv. Bragg. Nitrogen metabolism in the mutant seems to be disturbed, resulting in ureide accumulation.

Seed size influences the promoting activity of rhizobia on plant growth, nodulation and N fixation in lima bean

2021 ◽  
Vol 51 (3) ◽  
Author(s):  
Claudyanne do Nascimento Costa ◽  
Jadson Emanuel Lopes Antunes ◽  
João Pedro Alves de Aquino ◽  
Ingrid Sthephanie da Costa Silva ◽  
Angela Celis de Almeida Lopes ◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Size ◽  
Reference Strain ◽  
Dry Weight ◽  
Lima Bean ◽  
N Fixation ◽  
Nodule Number ◽  
Root Dry Weight ◽  
Biological N Fixation

ABSTRACT: This study evaluated the activity of rhizobia isolates inoculated in large (18 mm) and small (11 mm) seeds on lima bean growth, nodulation and N fixation. Selected rhizobia isolates were compared with a reference strain CIAT899 and two controls without inoculation. Large seeds contributed for highest plant growth, nodulation and N fixation than small seeds. The isolates UFPI-59, UFPI-18 and UFPI-38 promoted the highest values of shoot and root dry weight, respectively. The isolates UFPI-32 promoted the highest values of nodule number, while UFPI-59 promoted the highest values of nodule dry weight. The isolates UFPI-38 and UFPI-59 promoted the highest accumulation of N. This study showed that seed size really influences lima bean growth, nodulation and BNF. Considering rhizobia isolates, UFPI-59, UFPI-38, and UFPI-18 contributed for plant growth, promoted better nodulation and effectiveness on biological N fixation.

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