scholarly journals Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 143
Author(s):  
Marina Bottrel ◽  
Isabel Ortiz ◽  
Manuel Hidalgo ◽  
María Díaz-Jiménez ◽  
Blasa Pereira ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Reproductive Performance ◽  
Human Chorionic Gonadotropin ◽  
Recovery Rate ◽  
Ovulation Induction ◽  
Embryo Quality ◽  
The Other ◽  
Flushing Rate ◽  
Embryo Recovery ◽  
Embryo Grade

Two prostanglandins (luprostiol, LUP, and dinoprost, DIN) and two ovulation-inducing agents (human Chorionic Gonadotropin, hCG, and deslorelin, DES) were evaluated for luteolysis and estrus induction, and for ovulation induction, respectively, in embryo donor jennies. Twenty-six fertile Andalusian jennies were used. In Experiment 1, jennies (n = 112 cycles) were randomly treated with either LUP or DIN after embryo flushing. In Experiment 2, donors (n = 84 cycles) were randomly treated with either hCG or DES to induce ovulation. No differences were found between prostaglandins for all variables studied (prostaglandin–ovulation interval (POI), interovulatory interval (IOI), embryo recovery rate (ERR), positive flushing rate (PFR) and embryo grade (EG)). The ovulation rate was similar for hCG and DES (60.9% vs. 78.7%). However, the interval to ovulation (ITO) was affected (62.61 ± 7.20 vs. 48.79 ± 2.69 h). None of the other variables studied (ERR, PFR and EG) were affected (p > 0.05), except for embryo quality (p = 0.009). In short, both prostaglandins evaluated are adequate to induce luteolysis and estrus. Both ovulation-inducing agents hastened ovulation, but DES seems to be more effective than hCG. Follicular diameter affected the interval from treatment to ovulation, and high uterine edema was related to low embryo quality.

scholarly journals Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems

2008 ◽  
Vol 53 (No. 4) ◽  
pp. 145-151
Author(s):  
J.P. Ramon-Ugalde ◽  
J. Folch ◽  
M.J. Cocero ◽  
R.E. Piña-Aguilar ◽  
J.L. Alabart
Keyword(s):  
Recovery Rate ◽  
Uterine Horn ◽  
Conception Rate ◽  
The Other ◽  
Embryo Recovery ◽  
Intramuscular Injections ◽  
Other Hand

The efficiency of embryo recovery in a superovulatory treatment was studied by perfusing the oviduct or the uterine horn in 3.5 and 7 days after sponge withdrawal, respectively. Eighty-four and seventy ewes of Aragonesa breed were used as embryo donors and receptors, respectively. The donors were distributed in 3 replications. The oestrus was synchronized with the insertion of FGA intravaginal sponges (30 mg) for 13 days. Six intramuscular injections of 18 mg pFSH were applied in decreasing doses at 12 hour intervals starting 48 hours after sponge withdrawal. The recovery rate (RR) (83.2 vs. 75.8%), the viability rate (VR) (73.5 vs. 47.2%) and the number of viable embryos (VE) per donor ewe (5.9 ± 0.79 vs. 3.0 ± 0.37) were higher (<I>P</I> < 0.01) in ewes whose perfusion was done directly in oviducts. The interval from sponge withdrawal to oestrus had no influence on RR in any groups; however it had an effect on the VR in 3.5 and 7 days old embryos. Embryos from ewes showing the oestrus within 20 hours after sponge withdrawal had a higher viability. On the other hand, using morphologic criteria, 26.5% and 52.7% of 3.5 and 7 days old embryos, respectively, were discarded before transfer. The percentage of ewes in each group that became pregnant was similar in both groups. We conclude that in superovulated ewes the embryo recovery rate, viability and number of viable embryos are higher in embryos recovered from the oviduct compared to those recovered from the uterus, without decreasing the conception rate and the viability rate is higher in ewes that show the oestrus 20 hours before sponge withdrawal.

157 EFFECT OF DENSITY GRADIENT SELECTION ON EMBRYO RECOVERY RATES OF FRESH AND COOLED STALLION SEMEN

2013 ◽  
Vol 25 (1) ◽  
pp. 227
Author(s):  
F. Papa ◽  
M. Carmo ◽  
P. Papa ◽  
J. Dell'Aqua ◽  
M. Alvarenga
Keyword(s):  
Plasma Membrane ◽  
Density Gradient ◽  
Recovery Rate ◽  
Ovulation Induction ◽  
Membrane Integrity ◽  
Recovery Rates ◽  
Plasma Membrane Integrity ◽  
Embryo Recovery ◽  
Fresh Semen ◽  
Sperm Recovery

The aim of this study was to improve the spermatic kinetic parameters from stallions with poor quality of fresh and chilled semen by the use of density gradient Equipure™ (Nidacon, Mölndal, Sweden). Semen from 5 Quarter Horse stallions aged 8 and 16 years with history of low embryo recovery rates were used. The kinetics sperm evaluation was performed by computerized semen analysis (CASA) and plasma membrane integrity with fluorescent probes. The average motility parameters for fresh semen before selection were total motility (MT) 60%, progressive motility (PM) 30%, and plasma membrane integrity (IMP) 60% and for cooled semen (24 h at 5°C) were: MT 50%, MP 18%, and 50% IMP. For the group of fresh and cooled semen with no density gradient selection (NS), mares were inseminated with 1 billion viable sperm diluted in skim milk extender in the uterine body, 24 h after ovulation induction with 1 mg of deslorelin. For EquipureTM selection group (SE) semen was concentrated through Spermfilter membrane™ and resuspended in 5 mL of BotuSemenTM (BotupharmaTM, Brazil). In a 15 mL conic tube 5 mL of EquipureTM was added and another part containing 5 mL of the resuspended sperm was slowly added over the EquipureTM column. The 15 mL conic tube was centrifuged at 400g for 20 min. After centrifugation, the sperm pellet was carefully aspirated and to the pellet was resuspended in 4 mL of BotucrioTM. The sperm recovery rate with EquipureTM was 40%. Deep uterine AI was performed 24 h after ovulation induction with 1 mg of deslorelin. Data were analyzed by ANOVA followed by Tukey’s test (P < 0.05). The analysis of semen after EquipureTM selection resulted in average: 75, 35, 65, and 40%, respectively, for MT, MP, and IMP index and sperm recovery. The embryo recovery rate from the 5 stallions showed the following results: Stallion 1 (fresh semen): 12 mares (NS)/4 embryos (33%) and for group SE 22 mares/16 embryos, (72%; P ≤ 0.05). Stallion 2 (fresh semen): 9 mares (NS)/4 embryos, (44%) and for group SE, 12 mares/8 embryos (66%; P > 0.1). Stallion 3 (fresh semen): 4 mares (NS)/1 embryo (25%) and 7 mares for SE/4 embryos (57%; P > 0.1). Stallion 4 (chilled semen): 4 mares (NS)/0 embryo 0% and for SE group 8 mares/6 embryos 75%; P ≤ 0.001). Stallion 5 (chilled semen): 10 mares (NS)/2 embryos (20%) and for SE 6 mares/3 embryos (50%; P > 0.1). For the overall results, 39 inseminations were performed on the no selected group and 11 embryos recovered (28%) for the selected group 55 inseminations and 37 embryos recovered (67%; P < 0.01). The results clearly showed that selection by sperm density gradient EquipureTM was a very effective technique that allowed an improvement on semen quality and fertility. The authors acknowledge support from FAPESP and Botupharma.

scholarly journals Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1967
Author(s):  
J. Dorado ◽  
M. Bottrel ◽  
I. Ortiz ◽  
M. Díaz-Jiménez ◽  
B. Pereira ◽  
...  
Keyword(s):  
Developmental Stage ◽  
Embryo Transfer ◽  
Recovery Rate ◽  
Embryo Quality ◽  
Donor Age ◽  
Successive Cycle ◽  
Assisted Reproductive Technique ◽  
Factors Affecting ◽  
Embryo Recovery ◽  
Starting Point

Embryo transfer and the vitrification of embryos could be used for the conservation and recovery of endangered donkey breeds. It is important to develop techniques that optimize recovery rates and the cryotolerance of donkey embryos. This study evaluates factors affecting the recovery rate, quality, and diameter of embryos obtained from donor jennies as a starting point for the use of vitrification and embryo transfer in the conservation of the Andalusian donkey. A total of 100 embryos were recovered out of 124 estrous cycles (80.6%). The donor jenny affected the rates of positive flushings (PFR; p = 0.040) and embryo recovery (ERR; p < 0.05) as well as embryo quality (p = 0.004). ERR was also affected by the number of flushings (p < 0.001), donor age (p < 0.05), successive cycle within donor (p < 0.001), and jacks (p < 0.05). Number of flushings (p < 0.001) and jack (p < 0.05) had a significant effect on PFR, whereas the day of flushing influenced the developmental stage (p < 0.001), embryo quality (p < 0.05), and diameter of embryos (p < 0.001). The number of flushings significantly influenced the diameter (p = 0.038) and embryo developmental stage (p = 0.001), whereas the developmental stage was statistically different between herds (p = 0.020). The factors influencing the success of this assisted reproductive technique were donor jenny, donor age, successive cycle within donor, day of flushing, number of flushings, and jack. The identification of these key points is crucial to achieve a higher efficiency of embryo transfer and vitrification processes, before considering their application in the conservation of endangered donkey breeds.

Evaluation of neoadjuvant therapy in patients with nongerminomatous malignant germ cell tumors

2011 ◽  
Vol 7 (4) ◽  
pp. 431-438 ◽  
Author(s):  
Hideo Nakamura ◽  
Keishi Makino ◽  
Masato Kochi ◽  
Yukitaka Ushio ◽  
Jun-ichi Kuratsu
Keyword(s):  
Germ Cell ◽  
Neoadjuvant Therapy ◽  
Tumor Markers ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Tumor Recurrence ◽  
Germ Cell Tumors ◽  
Residual Tumor ◽  
The Other ◽  
Malignant Germ Cell Tumors

Object The authors evaluated the effectiveness of a neoadjuvant therapy (NAT) consisting of combined chemoand radiotherapy followed by complete resection of the residual tumor in patients with nongerminomatous malignant germ cell tumors (NGMGCTs). Methods The authors treated 14 consecutive patients in whom NGMGCTs were diagnosed based on elevated levels of the tumor markers α-fetoprotein, human chorionic gonadotropin, and the β-subunit of human chorionic gonadotropin (β-HCG). Chemotherapy and radiotherapy were performed, and after the serum tumor markers level was in the normal or near-normal range, the residual tumors were completely resected. Results Residual tumors were confirmed in 11 of the 14 patients after NAT, and total removal was successful in 10 of the 11 patients. In the other patient the residual tumor could not be completely excised because it was attached to a deep vein. The follow-up duration ranged from 1.2 to 22.2 years. The 5-year event-free and total survival rates were 86% and 93%, respectively. Although 3 patients died, 2 of tumor recurrence and 1 of a radiation-induced secondary tumor (glioblastoma), the other 11 are alive and without evidence of tumor recurrence. Conclusions The authors consider their NAT protocol for NGMGCT to be highly effective in relation to survival for the patients with NGMGCT, but there are several quality of life issues that need to be resolved.

scholarly journals Effect of pituitary extract, ovaprim and combination of human chorionic gonadotropin and metoclopramide on reproductive performance of Caspian shemaya, Alburnus chalcoides (Guldenstadt, 1772)

2019 ◽  
Vol 66 (1) ◽  
Author(s):  
Majid Nosrati ◽  
Hossein Khara ◽  
Habib Vahabzadeh ◽  
Sayeed Fakhredin Mirhashemi Nasab
Keyword(s):  
Chorionic Gonadotropin ◽  
Reproductive Performance ◽  
Human Chorionic Gonadotropin ◽  
Latency Period ◽  
Hormonal Treatment ◽  
Control Group ◽  
Pituitary Extract ◽  
Final Maturation ◽  
Experimental Treatments ◽  
Egg Diameter

The present study was conducted to examine the effects of three different hormonal treatment types i.e., pituitary extract (Pt), ovaprim (Ov) and human chorionic gonadotropin (HCG) + Metoclopramide (Met) on reproductive performance of Caspian shemaya, Alburnus chalcoides (Guldenstadt, 1772). To this end, three experimental treatments and one control group were considered. The experimental groups were administrated with different doses of Pt, Ov, HCG + Met as follows: Pt [2 mg kg bw (body weight)-1], Pt (3 mg kg bw -1), Pt (4 mg kg bw -1), Ov (10 μg kg bw -1), Ov (20 μg kg bw -1), Ov (30 μg kg bw -1), HCG + Met (1000 IU kg bw -1), HCG + Met (2000 IU kg bw -1), HCG + Met (3000 IU kg bw-1) and also a control group without any hormonal treatment. The highest values of oocyte weight and egg diameter were observed in groups administrated with Ov (10 μg kg bw-1) and HCG + Met (2000 IU kg bw-1) respectively. The highest values of absolute fecundity and relative fecundity were recorded for fish administrated with Ov (20 μg kg bw-1). The latency period and hour-degree for final maturation were lower in fish administrated with Ov (10 μg kg bw-1) compared to other experimental groups (p<0.05). There were no significant differences between experimental groups in terms of other assayed parameters. The results of the present study demonstrated higher efficiency of ovaprim in improving the reproductive performance of Caspian shemaya.

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